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All Journal Journal of Degraded and Mining Lands Management Research Journal of Life Science SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan
Syib'li, Muhammad Akhid
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Control & Systems Engineering Economics, Econometrics & Finance Education Engineering Environmental Science Health Professions Languange, Linguistic, Communication & Media Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Land use changes effect by slash and burn cultivation to understory diversity composition and soil degradation Setiawan, Adi; Pamungkas, Bayu Aji; Ito, Satoshi; Ramadhan, Varotama Putra; Lestariningsih, Iva Dewi; Khumairoh, Uma; Arifin, Syamsul; Wicaksono, Karuniawan Puji; Udayana, Cicik; Kurniawan, Yani; Tyasmoro, Setyono Yudo; Saitama, Akbar; Permanasari, Paramyta Nila; Syib'li, Muhammad Akhid; Aini, Luqman Qurata
Journal of Degraded and Mining Lands Management Vol. 11 No. 4 (2024)
Publisher : Brawijaya University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15243/jdmlm.2024.114.6153

Abstract

This study contributes to understanding ecological changes in land use following slash-and-burn land clearing. This study aimed to compare the richness and density of plant species and soil fertility in farmland cultivated for one year (1 Y), three years (3 Y), and five years (5 Y) after slash-and-burn, with the conditions in the secondary forest (SF) in Berau Regency of East Kalimantan. Understory plant taxon types, numbers, and soil organic matter were measured in each region using a series of nested plots. The size of each observation plot was 20 x 100 m. Data on understory vegetation composition was collected using 1 x 1 m mini plots at 20 sampling points and divided into five blocks, with a 10 m spacing between sampling points on each property. Understory vegetation species and cover percentage, litter cover percentage, open soil percentage, and soil fertility were measured for each observation plot. The results showed that the land cover percentage increased each year during the 5 years transition from secondary forest to farmland. Furthermore, within the 5 years, there was a change in understory species, particularly the presence of grasses and sedges at 3 and 5 years after the land use change. However, the transition from secondary forest to farmland within 5 years did not alter soil organic matter and total nitrogen.
Comparison of Primer Types to Produce Fungal DNA Fragment from Garlic Plant Root Samples Dewi, Fibrianti Shinta; Abadi, Abdul Latief; Syib'li, Muhammad Akhid
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.3

Abstract

Fusarium wilt disease caused by Fusarium oxysporum f.sp cepae is a widespread and dangerous diseases in garlic commodities. The combination of AMF and Bacillus amyloliquefaciens can be an effective solution to reduce losses due to attacks by these pathogens. The PCR technique is initial method that has proven to be significant in detecting fungal target DNA so that the effectiveness of biological agents can be determined. The aim of this study is to compare the types of primers that are effective for use in detecting fungal groups in PCR. This research was conducted in October 2023 - November 2024. The types of primers compared were ITS1-F/ITS4-R and LSU200A-F/LSU476-R with spectophotometry and fluometry quantification. The results show that ITS1-F/ITS4-R forms DNA band with a size of 700 bp, while LSU200A-F/LSU476-R had a size of 290-300 bp. The electropherogram also showed differences in DNA distribution which confirmed the results on agarose more specifically in the range 19-12,999 bp. In general, these results prove that the two types of primers have different abilities in fragmenting target DNA. This knowledge can help in detecting DNA at an early stage which can be used in various analyzes that can help in integrated plant disease management.
Pathogenicity Test and Molecular Characterization of Colletotrichum spp. Fungi Causing Apple Fruit Rot Disease in Malang Regency Maknunin, Lu'lu'il; Abadi, Abdul Latief; Syib'li, Muhammad Akhid
Research Journal of Life Science Vol 10, No 3 (2023)
Publisher : Direktorat Riset dan Pengabdian Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2023.010.03.4

Abstract

Apple (Malus sylvestris Mill) is a key commodity in Malang Regency, Indonesia, because of its role in promoting the agrotourism sector. But fruit rot caused by Colletotrichum spp. poses significant threats to production and its existence. This study aims to identify the causal agents of fruit rot in apple orchards in Pujon District, Malang, using morphological and molecular approaches. Four isolates suspected to be Colletotrichum spp. were obtained from symptomatic apple fruits and characterized through macroscopic, microscopic, and molecular methods, including ITS sequencing. Pathogenicity tests revealed that all isolates induced symptoms on healthy apples, with Colletotrichum siamense confirmed as the primary pathogen via BLAST analysis. These findings emphasize the need for accurate morphological and molecular identification to develop effective management strategies. Further research is recommended to examine the diversity of Colletotrichum species in Malang Regency to inform broader disease control strategies.
Penguatan sistem irigasi pertanian dan pengelolaan sampah melalui penataan kelembagaan berbasis resiprositas di Desa Kedungrejo, Kabupaten Malang Rozuli, Ahmad Imron; Pranowo, Dodyk; Nurdiani, Rahmi; Syib'Li, Muhammad Akhid; Purba, Dano; Renwarin, Ryan; Kusuma, Adhinugraha Wirayudha
SELAPARANG: Jurnal Pengabdian Masyarakat Berkemajuan Vol 9, No 4 (2025): Juli
Publisher : Universitas Muhammadiyah Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31764/jpmb.v9i4.31773

Abstract

AbstrakSistem irigasi dan pengelolaan sampah di Desa Kedungrejo menghadapi sejumlah tantangan serius, seperti praktik sabotase aliran air, penyumbatan saluran irigasi akibat sampah, serta rendahnya kesadaran warga terhadap kebersihan lingkungan. Kegiatan pengabdian melalui program Penataan Kelembagaan Terpadu Berbasis Resiprositas (P-KTR) dilakukan untuk mengatasi tantangan tersebut. Kegiatan ini melibatkan Pemerintah Desa, Paguyuban Pengelola Sampah, Kelompok Tani, Kuwowo, Waker, dan masyarakat sebagai aktor utama dalam sistem kelembagaan. Pemerintah desa berperan sebagai regulator dan fasilitator, sementara kelompok masyarakat bertanggung jawab atas implementasi teknis di lapangan. Hasil kegiatan menunjukkan peningkatan pemahaman dan keterlibatan masyarakat dalam pengelolaan irigasi dan sampah secara berkelanjutan. Keunggulan model ini terletak pada integrasi lintas sektor, partisipasi kolektif, dan insentif warga berbasis kontribusi. Tantangan yang tersisa mencakup rendahnya kedisiplinan iuran sampah serta perlunya penguatan mekanisme koordinasi antaraktor. Pengembangan kelembagaan ke depan perlu memprioritaskan peningkatan kapasitas teknis, digitalisasi sistem pemantauan, dan penguatan kolaborasi berkelanjutan dengan institusi akademik dan pemerintah daerah. Penerapan model ini secara konsisten diharapkan mampu mendorong pertanian yang lestari, lingkungan desa yang lebih bersih, dan kesejahteraan masyarakat yang berkelanjutan. Kata kunci: irigasi; pengelolaan sampah; kelembagaan; resiprositas. AbstractThe irrigation and waste management systems in Kedungrejo Village face several serious challenges, including the sabotage of water flow, blockage of irrigation channels due to waste, and low public awareness regarding environmental cleanliness. The community service activities through the Reciprocity-Based Integrated Institutional Arrangement (P-KTR) program were conducted to address these challenges. The initiative engages key stakeholders in the institutional system, including the Village Government, Waste Management Association, Farmer Groups, Kuwowo (village irrigation managers), Waker (water gate operators), and local community members as primary actors. The village government acts as a regulator and facilitator, while community groups are responsible for technical implementation in the field. The results of the program indicate an increase in community understanding and involvement in sustainable irrigation and waste management. The strength of this model lies in its cross-sectoral integration, collective participation, and citizen incentives based on contribution. Remaining challenges include low discipline in waste fee payments and the need to strengthen coordination mechanisms among actors. Future institutional development should prioritize technical capacity building, digital transformation of monitoring systems, and strengthening sustainable collaboration with academic institutions and local governments.Consistent implementation of this model is expected to promote sustainable agriculture, a cleaner village environment, and long- term community well-being. Keywords: irrigation; waste management; institutional arrangement; reciprocity.
Co-Authors Abdul Latief Abadi Achmad Noerkhaerin Putra Ahmad Imron Rozuli Dewi, Fibrianti Shinta Dodyk Pranowo Ito, Satoshi Iva Dewi Lestariningsih, Iva Dewi Karuniawan Puji Wicaksono Kusuma, Adhinugraha Wirayudha Luqman Qurata Aini Maknunin, Lu'lu'il Pamungkas, Bayu Aji Paramyta Nila Permanasari, Paramyta Nila Purba, Dano Rahmi Nurdiani Ramadhan, Varotama Putra Renwarin, Ryan Saitama, Akbar Setyono Yudo Tyasmoro Syamsul Arifin Udayana, Cicik Uma Khumairoh Yani Kurniawan, Yani