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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 8 Documents

Search results for , issue "Vol 3, No 3 (2016)" : 8 Documents clear

Financial Analysis Of Citrus “Keprok 55” In Coventional Way and Using Sprinkler Irrigation Bambang Suharto; Liliyan Dewi Susanawati
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Batu-Malang Government area is one of central production of citrus ”Keprok 55” in East Java. In dry season, citrus produce low production level, because there is unsufficient water inside the soil. Based on this facts, some efforts is needed in order to increase the citrus production especially to face the dry season, through sprinkle irrigation system to increase citrus production. Sprinkle irrigation system has been installed on citrus plantation in Selorejo Village, Batu.Citrus is one of the horticultural commodities which have priority to be developed. One type of citrus that favored consumers are mandarin (tangerine). During the period 2005-2009 tangerine imports reached 504,063 tonnes, or about 100,813 tonnes per year with a value of US $ 80,569,300. Management of commodity Citrus 55 in Selorejo village, subdistrict Dau, Malang not get maximum benefit due to the limitations of the use of technological innovation as well as the lack of capital. The experiment calculated HPP, BEP, NPV, R/C ratio, IRR and PP. This study was to compare the effect of management changes Citrus 55 between conventional and sprinkler irrigation. The financial viability of commodity management Citrus 55 in Selorejo Village declared eligible to run either conventionally or with sprinkler irrigation. Age commodity management business is ten years. Conventional management NVP value is IDR 1,234,468,408 and the value of NVP management with the use of sprinkler irrigation is IDR 5,200,599,957. PP value of conventional management is 5 years 2 months and sprinkler irrigation is 2 years and 5 months. Conventional management IRR value is 59.47% and value of management use sprinkle is 68.08%. R/C Ratio with conventional management is 1.4 and value R/C Ratio with sprinkle is 5.7. BEP value with management conventional is 5,719 kilograms and with sprinkle is 16,059 Kilograms. HPP value with management conventional is IDR 784 and with sprinkle is IDR 191.

Extraction of Glycosaminoglycans Containing Glucosamine and Chondroitin Sulfate from Chicken Claw Cartilage Tri Dewanti Widyaningsih; Widya Dwi Rukmi; Erni Sofia; Sudarma Dita Wijayanti; Novita Wijayanti; Rika Ersalia; Nia Rochmawati; Debora Nangin
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Chicken cartilage (claw) is a waste of chicken cuts which are widely available in Indonesia. Cartilage part of chicken claw becomes a potential source of chondroitin sulfate (CS) and glucosamine (GS). This study aims to determine the most optimal extraction methods of CS and GS from cartilage of chicken claw. Various types of extraction methods used in this study are taken from the extraction by using boiling water (2 and 2.5 hours), acetic acid (7 and 17 hours), as well as proteolysis by papain (24 and 48 hours). Parameters observed include chemical characteristics of powdered cartilage of chicken claw as well as CS and GS levels in powdered cartilage of chicken claw extract. The results of this research show that the levels of CS and GS of chicken claw cartilage powder were 2.17% and 13%. Meanwhile, the highest GS level was obtained from the extraction with water treatment for 2.5 hours which was 8.1%. The treatment and duration of extraction will significantly affect the number of GS which was produced. The highest content of CS was obtained from the extraction with the enzyme treatment for 48 hours which was 2.47%. The best treatment is the extraction with water treatment for 2.5 hours which were the extracts with GS levels of 8.1% and 2.03% CS was selected through the analysis of multiple attribute.

Identifying and assessing Spice and herbs resources for tourism attractions: A case study from Banyuwangi, East Java Luchman Hakim; Soemarno Soemarno; Bagyo Yanuwiyadi
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The aims of the study was to analyze the potentiality of spices and herbs in agrofotestry garden in Banyuwangi, Indonesia, for further rural tourism development. Fields study was set up at villages in Banyuwangi. Floristic survey, ethnobitanical studies and focus group discussion were performed to evaluate the value of spices and herbs in agroforestry gardens an its potentiality to develop as tourism product. This study found that at least 60 species of spice and herbs with its special value, in which these species has opportunities to developed as tourism attraction in agroforestry garden. There are something to see, something to buy and something to do has been described by group discussion participant. It is raging from farm tourism product, culinary tourism product and heath-wellness tourism product.

Immunomodulation Effects of Bryophyllum Pinnatum on Pregnant Pristane-Induced Lupus Mice Model Nurdiana Nurdiana; Elvira Sari Dewi; Yuni Sari; Rahmawati Wahyuni; Aminah Maya; Nursari Abdul Syukur; Mirza Zaka Pratama; Wisnu Barlianto; Umi Kalsum; Kusworini Handono
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Objective: To determine the effect of Bryophyllum pinnatum treatment in modulating immune response and the pregnancy outcomes of pregnant pristane-induced lupus mice model.Methods: Sixteen Balb/c mice were intraperitoneally injected with single 0.5 cc pristane to induce lupus manifestations. After 12 weeks of injection, mice were mated and considered as gestational day 0 (GD0). Mice were divided into 4 groups based on the dosages of Bryophyllum pinnatum: control (no treatment), B1 (10.5 mg/kg), B2 (21 mg/kg), and B3 (42 mg/kg). The treatment was given orally every day started from GD9 until 9 days. At the end of the study, blood pressure and fetal size were measured. Serum anti-dsDNA and urine albumin levels were measured by ELISA. Spleen T helper (Th) and mature B cells percentages were measured by flow cytometry.Results: Administration of Bryophyllum pinnatum reduced the percentages of Th1 (p=0.006), Th2 (p=0.005), Th17 (p=0.000), and mature B cells (p=0.007) in dose-dependent manner. B1 and B2 had significantly lower of systolic blood pressure compared to control (p=0.026 and p=0.022 respectively). Significantly lower of anti-dsDNA levels were found in B1 group compared to control (p=0.014). However, no significantly different of urine albumin levels were found between groups. Bryophyllum pinnatum also significantly increased the fetus body weight in dose-dependent manner (p=0.000).Conclusion: Treatment of Bryophyllum pinnatum could improve the pregnancy outcome and modulate the immune response in pregnant pristane-induced lupus mice. Therefore, Bryophyllum pinnatum is a potential herb which can be developed as an immunosuppressive agent in the future.

Prebiotics Activity of Laminaran Derived From Sargassum crassifolium Anies Chamidah; Hardoko Hardoko; Asep Awaludin Prihanto
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The objectives of this study were to evaluate the prebiotic activity based on the change in cell biomass of the probiotic strain after 24-h growth in the presence of Laminaran from Sargassum crassifolium with the methods of laminaran acid extract (LAE) and laminaran modified extract (LME), inulin, or glucose-relative against the change in cell biomass of Escherichia coli FNCC 0091 grown under the same condition. Prebiotic activity was calculated for L. plantarum FNCC 0051 and Bifidobacterium longum FNCC 1081. The results showed that the increasing cell number of L. plantarum was higher in both substrates LAE and LME (0,58 and 2,03log cycle), whereas that of B. longum was lower. The higher prebiotic activity score obtained for L. plantarum and B. longum grown on LME were positive (0,26 and 0,96 log cycle), whereas the lowest score was for L. plantarum and B. longum grown on LAE, which were negative (-0,35 and -0,31 log cycle), but the higher prebiotic activity score was obtained for inulin (4,08 and 4,78 log cycle). It could be concluded that Laminaran Modified Extract (LME) has potential as prebiotic source, but its potential was lower than inulin.

The Effect of Photoperiod to Break Dormancy of Porang’s (Amorphophallus muelleri Blume) Tuber and Growth Serafinah Indriyani; Wahyu Widoretno
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The aim of the research was to obtain the method of porang’s tuber dormancy breaking. The source of porang’s tuber were obtained from Rejosari Village, Bantur Subdistrict, Malang Regency. The diameter and weight of porang’s tuber were 5-7 cm and 100-150 g respectively. The research design was Completely Randomized Design. Porang’s tubers were storaged in the photoperiod cabinet during one month. The levels of photoperiod were 0, 8, 12, 16, 20, and 24 hours/day. The light intensity of TL lamp was 400 lux. As a control it was used porang’s tuber that it was placed in the dark cabinet without light. Repetition was four times. The success of dormancy breaking was observed through the tuber capasity to grow that it was identified by bud emerged. Data were analyzed by ANOVA that it was continued by Duncan test (α = 0.05). The results showed that photoperiod 8, 12, 16, 20, and 24 hours/day during one month could stimulate porang’s tuber dormancy breaking. Photoperiod 16 hours/day emerged the height of buds 4.97 ± 1.20 mm and the diameter of buds 7.28 ± 1.22 mm at photoperiod 24 hours/day. The growth of porang’s tuber which is treated by photoperiod during one month were significantly different with control. Photoperiod 16 hours/day emerged the height of plants and the diameter of petiole were 106.38 ± 15.11 cm and 2.90 ± 0.29 mm respectively, while the width of canopy was 72.50 ± 22.17 cm at photoperiod 24 hours/day. Besides, the diameter and weight of harvest tuber were 7.93 ± 2.20 cm and 383.20 ± 23.58 g. The weight of harvest tuber increased 255% from the early weight of tuber. Photoperiod treatment promote breaking of porang’s tuber dormancy.

Stock Identification Sardinella Lemuru, Bleeker 1853 In Madura Strait, Bali Strait, and Southern-East Java Water Setyohadi, Daduk; Iranawati, Feni; Hikmah, Syarifah
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Balinese Sardinella (Sardinella lemuru) is the main commodity in Indonesia. Despite, more research show that lemuru got the overfishing condition. In East Java region, likely Bali Strait, Southern-East Java water, and Madura Strait are the region which the most production of lemuru catch. So it needs identification stock of Sardinella lemuru on the third fisheries management east java sub-region, as it to be used as a reference to managing the sustainability of the catch sardinella lemuru for the future. Research carried out in January to May 2016 with the methods is the sample of random sampling on three different locations water representing Madura Strait, Bali Strait, and southern-East Java water. The each sample implemented were in four times during a period of months old different. Every the sample researchers get as 50-200 tail. Sardinella lemuru in accordance with the season fish. The sample which has been obtained taken to the laboratory of Marine Science University of Brawijaya to data take. The analysis used in this research was Truss morphometric and Factor analysis of the condition allometric. Based on the results of the analysis in the Madura Strait, Bali Strait, and Southern-East Java water are having a distinction of morphometric character amount 44 % with the character being very affected is PAL_FL, DFB_HL , PVL_FL, UPCL_FL, OD_HL, and PPL_FL. Around 64 % of the whole morphometric character that is 14 characters. Next, if it is seen from Allometric condition in the third these waters having b value different from each aquatic and after B-test that lemuru in the three the waters might otherwise dissimilar stock so management of Sardinella lemuru in the three waters should have been undertaken in separated.

Saxitoxin Level Comparation in Bali Sardine (Sardinella Lemuru) in Bali Strait in Different Monsoons Endang Yuli Herawati; Mohammad Mahmudi; Fani Fariedah; Ruly Isfatul Khasanah
Research Journal of Life Science Vol 3, No 3 (2016)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Sardinella fish (Sardinella lemuru) is a species of important pelagic fish from the family of Clupeidae found in Bali Strait. The dynamic condition of Bali Strait makes it fertile which is identical with plankton blooming. The objective of this research was to understand the potential of saxitoxin in sardinella fish because of dinoflagellates consumption in Bali Strait. The research was conducted in Southeast Monsoon (June - August 2015) and Northwest Monsoon (December 2015 – February 2016). The method used was in situ plankton sampling, counting and ELISA test to determine the level of saxitoxin in the fish. Hydro-oceanographic parameters measured were temperatures, salinity, DO (dissolved oxygen), pH level, phosphate level, nitrate level and transparency. They showed average optimum value for phytoplankton growth especially during Southeast Monsoon which its temperature lower and the nutrients (phosphate nitrate) higher than the Northwest Monsoon. This condition caused high richness of phytoplankton in the water and followed with dinoflagellates richness so they accumulated in sardinella fish. It was supported by the analysis of the fish gastric which showed positive correlation between dinoflagellates richness in the water and the fish gastric. ELISA test also showed that saxitoxin level of fish caught in Southeast Monsoon was higher than the one caught in Northwest Monsoon even though the level of saxitoxin was still in the safe range (less than 80 μg STXeq. per 100 g) but the danger of saxitoxin accumulation should be watched out for.

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Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location Kota malang, Jawa timur INDONESIA

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Kuswanto

Contact Email
rjls@ub.ac.id

Phone
+62341 575825

Journal Mail Official
rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA