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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 7 Documents

Search results for , issue "Vol 5, No 2 (2018)" : 7 Documents clear

Effect of Endurance Treadmill Training on mIGF-1 Expression and PAX 7 Satellite Cell in Rat Muscle Tissues Mustofa Mustofa; Karyono Mintaroem; Agustina Endharti
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

This study examined the effects of endurance exercise training on muscle Insulin Growth Factor-1 (mIGF-1) and PAX7 transcription factor of satellite cell activation in a tibialis anterior muscle of rat. Rat were subjected to treadmill, divided into three groups, each containing 6 rats. Groups of high, moderate and low doses of exercise received 28, 17 and 12 m/s of treadmill speeds respectively. Treadmill exercise training conducted in 5 days a week within 9 weeks. At the end of 9 weeks of the experiment, rat were sacrificed, tibialis anterior muscle tissues was removed and then subjected to immunohistochemistry examination. There were significant differences in intensity of training in mIGF-1 and PAX7 in muscle tissues (P < 0.05). These results suggested that endurance exercise training which high, moderate and low intensity able to increase mIGF-1 and PAX7 that associates with satellite cell development of muscle tissues.

Turmeric (Curcuma longa) Decoctum Had no Effect on Vascular Endothelial Growth Factor (VEGF) and Vascular Endothelial Cadherin (VE-cadherin) Expression in the Chick Embryo Sri Winarsih; I Wayan Arsana Wiyasa; Sri Andarini; Sumarno Reto Prawiro
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Turmeric (Curcuma longa) as a medicinal plant has widely consumed by pregnant women. The absence of dose standardization and the regulation of turmeric consumption in pregnancy raise concerns, especially if taken in early pregnancy. Angiogenesis is the process of new blood vessel formation from previously existing blood vessels, and plays an important role in embryogenesis and placentation during pregnancy. Angiogenesis is regulated by angiogenic molecules, such as VEGF and VE-cadherin. Turmeric has antiangiogenic effects in which the crude extract is pharmacologically more potent compared to the pure curcumin form. This study aimed to determine whether a turmeric decoctum affects the expression of VEGF and VE-cadherin in chick embryos. Turmeric was extracted by the decoction and freeze dried methods to obtain turmeric decoctum powder. This was tested on embryonated chicken eggs, which were divided into four groups; control group (2% DMSO) and treatment groups receiving various doses of the turmeric decoctum (200 ppm, 300 ppm and 400 ppm). The eggs used were less than 7 days after oviposition and incubated for 16 hours prior to injection of the treatment solution in ovo to the center of the yolk, followed by reincubation for up to 48 hours. Intraembryonic VEGF and VE-cadherin expression were assessed by whole-mount immunohistochemistry and quantified using image analysis techniques. This study showed administration of turmeric decoctum up to 400 ppm had no effect on the expression of VEGF and VE-cadherin in chick embryos.

The Refugia Attract Arthropods in a Paddy Field in Malang, East Java, Indonesia Amin Setyo Leksono; Jati Batoro; Anisa Zairina
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

A study on the effect of refugia areas to attract on Arthropods has been done in a semi-organic paddy field in Malang, from March to June 2017. The arthropod was measured by abundance, diversity and composition variables. Arthropod observations were performed on 6 plots of 1 m2 each, consisting of 3 plots on the side near the Refugia area (treatment) and 3 plots on the opposite side (control). The refugia areas consisted of plants as follows chili (Capsicum frutescens), the wild cosmos (Cosmos caudatus), the long beans (Vigna unguiculata), and Marigold (Tagetes erecta). The visual encounter surveys method was conducted for 15 minutes on each plot. There were 2249 individuals of Arthropod observed visually in the study sites. This study showed that of the abundance (mean ± SE) of Arthropod visitors was lower in plots adjacent to refugia areas (treatment) (33.7± 2.63), than that far from Refugia area (control) (38.33 ± 2.54); while the taxa richness and diversity was higher found in treatment plots. The species richness in the treatment plots (12.2 ± 0.70) was higher than that in control (9.70 ± 0.51). The diversity of Arthropod visitors in the treatment plots (2.10 ± 0.07) was also higher than that in control (1.71 ± 0.07). Proportion of predator in the treatment plots was almost twice higher (33.14) that in the control plots (17.65); while that of herbivore was vice versa. This meant the refugia areas have attracted more predators. The composition of Arthropod visitors was remarkable affected by treatment. The abundance of common predator families such as Coccinellidae, Coenagrionidae and Oxyopidae were higher in treatment plots.

Allele Frequencies of STR CODIS 13 of Madura Ethnic from Bangkalan and Probolinggo Wening Prastowo; Diana Lyrawati; Sri Andarini; Karyono Mintaroem
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

One of forensic identification that can be done is through DNA testing. FBI recommends to use 13 short tandem repeat loci known as CODIS 13 for forensic DNA testing. We tested such system to characterize and determine whether individuals with Madura ethnic background (Madurese) resides in different region differ in CODIS 13 pattern. Employing standard PCR-RFLP for STR CODIS 13 our results showed that indeed the CODIS 13 pattern between the two regions were distinct. Most frequent alleles appeared in Madura ethnic from Bangkalan were at locus D3S1358: allele 16; VWA: 16; FGA: 24 and 26; TH01: 8; TPOX: 9; CSF1PO: 10; D5S818: 11 and 13; D13S317: 14; D7S820: 9; D8S1179: 15; D21S11: 27, 28 and 30; D18S51: 16; and D16S539: 15. Most frequent alleles appeared in each locus of Madura ethnic from Probolinggo were locus D3S1358: allele 17; VWA: 17; FGA: 23 and 26; TH01: 7; TPOX: 10; CSF1PO: 10, 11 and 13; D5S818: 11 and 12; D13S317: 11; D7S820: 11; D8S1179: 11 and 12; D21S11: 30; D18S51: 14; and D16S539: 8. The heterozygosity levels of Madura ethnic from Bangkalan’s CODIS 13 varied from 0.4180 (D5S818) to 0.9102 (FGA). The power of exclusion was between 0.1569 (D5S818) to 0.7667 (FGA). The power of discrimination was between 0.2816 (D5S818) to 0.9811 (FGA). The paternity index was between 0.8591 (D5S818) to 5.5653 (FGA). The heterozygosity index of Madura ethnic from Probolinggo varied between 0.63021 (VWA) to 0.94445 (FGA), power of exclusion was between 0.33418 (VWA) to 0.84779 (FGA), and power of discrimination ranged from 0.60501 (TPOX) to 0.99305 (FGA).

Hematological Analysis of Nile Tilapia (Oreochromis niloticus) and Striped Catfish (Pangasius hypophthalmus) using Hematology Analyzer Tool Software at Fish Breeding Center Jojogan, Tuban, East Java Maftuch Maftuch
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Nile tilapia and Striped Catfish can be classified as freshwater fish species that have great cultivation potential. Inadequate cultivation management leads to the occurrence of disease attacks on fish. Hematological examination was applied to determine the level of fish health. This research was aimed to know the difference between erythrocyte cell and leucocyte cell count by using Hematology Analyzer Tool Software (computer based) and hand tally counter (human based), and to know the description of hematocrit component, hemoglobin and leucocytes differential on tilapia fish and Striped Catfish. The methods employed in this research were experimental method and descriptive method. The results showed that the calculation of erythrocyte cells and leucocyte cells by using Hematology Analyzer Tool Software was similar to the calculation by using hand tally counter. It was because the result of t value was smaller than t of the 5% table. Based on the description of the hematocrit component, hemoglobin and leucocyte differentials, it could be said that nile tilapia and Striped Catfish were within the range of abnormalities.

Analysis of The Secondary Metabolite of Kersen Leaf Extracts (Muntingia calabura L.) and Its Potential as Anti-Bacteria to Inhibit Aeromonas hydrophila Gede Angga Krishna Fariestha; Sri Andayani; Uun Yanuhar
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Aeromonas hydrophila is a gram-negative bacterium that massively attack aquatic organisms in fresh water, briny water and sea waters. Infection due to this bacterium caused septicemia in the host's body to the point of damaging the body's organs. Hence, alternative material is required in order to cope with this bacterium, which was by using natural material such as kersen leaf (Muntingia calabura L). The purpose of the research is to determine the effect of secondary metabolite contained in kersen leaf as anti-bacterial against Aeromonas hydrophila. This research used a method of MIC (Minimum Inhibitory Concentration) as an anti-bacterial test. In order to examine the secondary metabolite content, phytochemical screening and FTIR (Fourier Transform Infrared Spectroscopy) methods were used. The results obtained through the MIC test of 125 ppm is the minimum concentration capable of inhibiting the growth of Aeromonas hydrophila. Kersen leaf extracts is bacteriostatic anti-bacteria which only inhibit bacterial growth up to 24 hours. Phytochemical screening reported that kersen leaf extracts were positive of flavonoids, tannins, saponins, alkaloids and triterpenoids. Through FTIR test, it is known that phenol compounds were contained in kersen leaf extracts. This phenol is one of the metabolites with the function of anti-bacterial on this study.

Influence of Light Intensity on Lipid Productivity and Fatty Acids Profile of Choricystis sp. LBB13-AL045 for Biodiesel Production Swastika Praharyawan; Delicia Yunita Rahman; Dwi Susilaningsih
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The influence of various light intensity on the lipid productivity, fatty acids profile, and biodiesel properties of Choricystis sp. AL045 were examined. Choricystis sp. LBB13-AL045 was a high lipid content microalgal strain (up to 42.49 ± 0.41% per dry weight basis) with great performance of its growth (specific growth rate and biomass productivity were up to 0.802 ± 0.013 day-1 and 108.57 ± 8.07 mg L-1 day-1, respectively). Such results indicated its high lipid productivity and its potency to be used for biodiesel production. The treatment of various light intensities on the microalgal culture resulted obvious differences in lipid productivity and fatty acids composition. Maximum lipid productivity (46.13 ± 3.43 mg L-1 day-1) was at 405 µmol m-2 s-1 of light intensity. The fatty acids profiles of four light intensities treatments were quite similar; the major components of fatty acid obtained from Choricystis sp. LBB13-AL045 oil were oleic acid (C18:1) and palmitic acid (C16:0), which provided a strong indication of high-quality biodiesel. Biodiesel properties were determined by empirical equations and found to be within the limits of biodiesel standard SNI 7182:2015, ASTMD6751-08 and EN14214.

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Kuswanto

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Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

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Kota malang,

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Contact Name Kuswanto

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Journal Mail Official rjls@ub.ac.id

Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Kuswanto

Contact Email
rjls@ub.ac.id

Phone
+62341 575825

Journal Mail Official
rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA