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Articles 14 Documents
Search results for , issue "Vol 1, No 4 (2012)" : 14 Documents clear
T Cell Donor Type Regulator Required to Prevent Failure of Bone Marrow Transplantation of Alogenic System on Model C57BL / 6 → BALB / c Muhaimin Rifa’i
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (104.651 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.11

Abstract

In solid organ and bone marrow transplantation, rejection of donor tissue by recipient and graft versus host disease (GVHD) reactions are the most important issues in the health field. Furthermore, it is known that the tolerance between donor and recipient cannot be maintained for long term without manipulation. To determine the role of regulatory T cells, in this experiment we conducted infusion of donor type CD4+CD25+ T cells. Before transplantation was performed, recipients were irradiated with lethal doses of 850 rad. In this study, we demonstrated that the infusion of donor type CD4+CD25+ regulatory T cells in transplantation model of C57BL/6 → BALB/c promoted the tolerance of recipient against donor. Incontras, conventional T cells CD4+CD25- cannot help the success of transplantation at three months post-transplantation. Rejection of donor tissue by the recipient and GVHD reaction are caused by the activation of T cells and lack of regulatory T cells, CD4+CD25+. These results suggest that CD4+CD25+ regulatory T cells play an important role to homeostasis maintaining and keeping the number and function of lymphocyte T cells in normal circumstances. Furthermore, this study demonstrated that donor CD4+CD25+ regulatory T cellsexpress high levels of Foxp3 molecules.
Study on the Preparation and Liquid Smoke Fluids Characterization as the Results of Pyrolysis from Sawdust Sri Sugiarti; M. Nurhuda
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (130.857 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.12

Abstract

There is a huge amount of biomass resources but it has not been utilized properly. For instance, converting it into liquid smoke. Liquid smoke is produced by pyrolysis process from four differences sawdust i.e. teak (Tectona grandis sp), mahagony (Switenia mahagoni jacq), jackfruit (Artocarpus heterophyllus lamk), and red sandalwood (Pterocarpus indicus). Pyrolysis process required 750-gram sawdust and performed for approximately 40 minutes.Percentage of liquid smoke production ranged from 26.51 ± 0.009% (mahagony) – 32.12 ± 0.009% (jackfruit). While, the charcoal produced ranged from 23.54 ± 0.008% (mahagony) – 34.76 ± 0.009% (jackfruit). The density value of liquid smoke produced ranged between 1014.996 ± 0.346 kg/m3 (mahagony) – 1019.408 ± 0.037 kg/m3 (teak).
Study of Package Fraction Fraction Sensitivity TRISO in Design of HTR Pebble Bed Criticality Zuhair Zuhair; Suwoto Suwoto
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (145.502 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.13

Abstract

HTR is a high temperature reactor used for electricity production and process heat applications such as hydrogen production, desalination of sea water, enhanced oil recovery and so on. HTR is designed based on the utilization of TRISO fuel particles that can prevent strongly the escape of fission products even at temperatures above 1600 oC. TRISO particles packing fraction is one of four key parameters that are essential in HTR design besides radius of the kernel, kernel density and fuel enrichment. This paper discusses the sensitivity of TRISO particles packing fraction that impacts to the loading of uranium in the fuel pebble, the long cycle of reactor operation and achievable maximum fuel burn-up. With the capability of Monte Carlo transport code MCNP5, all components of the reactor, starting from TRISO particles, were modeled in detail and explicit and calculated using the continuous energy nuclear data library ENDF/B-VI. The results show that the value of effective multiplication factor (keff) has a tendency to increase with decreasing particle TRISO packing fraction and to decrease with increasing fuel burn-up. Keff values decrease with increasing TRISO particle packing fraction both at the beginning of cycle (BOC) and at the end of cycle (EOC). Reactivity swing is also very sensitive on the TRISO particles packing fraction. From the analysis, it can be concluded that TRISO particles packing fraction greatly affects the neutronics performance of HTR pebble bed design. Packing fraction can change the effective multiplication factor (keff) and the swing reactivity with similar behavior.
Allergenity Test of Porang Bulb Variant (Amorphophallus oncophyllus Hook.) By ELISA Ig-E Method Sofy Permana; Sri Widyarti; Serafinah Indriyani; Dian Siswanto; Aris Soewondo
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (70.475 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.14

Abstract

Porang (Amorphophalus oncophyllus Hook.), a tuber plant, is intensively explored for export commodities from East Java and as a raw food stuff material for domestic industry. The prospects of Porang tubers as the healthy food is interesting, however some studies are needed for their allergy effects. It was thought that the allergy effects of Porang are caused by the composition of Calcium Oxalate crystal that leads the itching and irritation in the mouth. On the other hands, the food allergy is usually caused by unchanged protein during food digesting.  Some proteins have been known that play a role in Calcium Oxalate crystal (Matrix proteins) formation, they are: Asp-rich and Ser-rich acidic proteins that are tightly bound to Calcium. So, substantially the allergen of Porang tuber is from the matrix protein, not from its crystal. The allergen in the food is usually a protein that is not change during the food digestion. In this study, it has been done the allergic test of some Porang tuber variants, namely: Sumber Baru-Jember; Klangon, Saradan-Madiun and Sumber Bendo, Saradan-Madiun, by ELISA-IgE from rat’s blood serum that have been orally treated by raw Porang tubers powder before.  The SDS-PAGE analysis shows that the proteins bands profiles are similar between raw and steamed Porang tubers (appearance Molecular Weight: 18 and 15 kDa). But, those raw Porang tubers bands are thicker than the steamed ones. The thickness of those raw Porang tubers decrease from 25 to 50 %, compare to those steamed ones. It means that the steaming of Porang is effective to decrease the potential allergy of Porang for consumptions. The allergic test analysis by ELISA-IgE revealed that the potential allergy of Porang are: Sumber Baru (Jember) > Porang var. Klangon - Saradan (Madiun) > Porang var. Sumber Bendo - Saradan (Madiun), respectively.
Applications of Cellulolytic Microorganisms and Watering Frequency in Early Seedling of Palm Oil in Peatlands Gusmawartati Gusmawartati
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (99.647 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.1

Abstract

This research aimed to determine optimum water necessity by using cellulolytic microorganism to enhance growth of oil palm on pre-nursery in peat soil. The research was conducted at an area with peat soil as growth media was taken from desa Rimbo Panjang Kabupaten Kampar Province of Riau by Factorial Completely Randomized Design with three replication. The first factor consists of: 0, 10, 20 and 30 (mL/polybag) of cellulolytic microorganism. The other one consists of: 2, 3 and 4 (times/day) watering. The result  showed that  cellulolytic microorganism with frequency of watering could improve peat soil fertility and increased growth of oil palm in pre-nursery. Use of 30 ml cellulolytic microorganism with 2 times watering/day decreased  C/N ratio till 26% and increased 1–1.5 of soil pH and created the best growth of oil palm seed, is equal to standart wich is recominded by the  central  oil palm research of Indonesia.
Design of Cell Construction for Immunosensor Based Quartz Crystal Microbalance (QCM) Farida Wahyuni; Setyawan Purnomo Sakti; Unggul P. Juswono; Fenny Irawati; Nur Chabibah
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.764 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.2

Abstract

The biosensor is a sensor device that combines biological compounds with a transducer. One type of biosensor that uses mass change detection techniques are QCM (Quartz Crystal Microbalance). QCM is a sensor that works with the principle of quartz crystal frequency shift due to mass deposition on the surface of the crystal. QCM can be used to detect the reaction between the molecules, so that the QCM can serve as biosensors that can be used for the diagnosis of a disease. In the development of QCM immunosensor for there are many problems, one of which is the construction of the cell. Construction cells can be used as a reaction between biomolecules. Construction of cells made of white Teflon. To keep QCM sensors are not experiencing physical stress that can lead to rupture due to pressure from the Teflon o-ring is used as an insulating silicon. The results of this study indicate that the construction of the cells created can be used as a medium for immobilization of Bovine Serum Albumin observations (BSA) on the surface of the sensor.
Optimal Control Design of Eco-Friendly Power Generators Using Wind Power Ahmad Nadhir; Agus Naba
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (59.557 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.3

Abstract

Two optimal control methods based on fuzzy inference system (FIS) for maximizing extraction of energy in wind energy conversion system (WECS) is already presented. An MPPTFIS is a first optimal control method using maximum power point tracking approach and fuzzy system. The objective of MPPTFIS is to make zero value change rate of power and rotor speed. A control system will drive an actuator to increasing or decreasing  the generator speed depend on the measurement rate of power and rotor speed. An optimal of WECS can be achieved by carried through the rate of power and rotor speed that operating near optimal point. The second optimal control method is proposed by using adaptive neuro fuzzy inference system (ANFIS) to finding model of power curve that will be applied for design of linear control feedback (LCANFIS). The advantage of LCANFIS than MPPTFIS is only one parameter measusrement needed: wind speed. MPPTFIS and LCANFIS could maximize extraction of the wind energy that verified by a power coefficient Cp stay at its maximum almost all the time and an actual power line close to a maximum power extraction (MPE) line reference during simulation process using a same of wind profile.  
Patchouli Oil Characteristics by Optimization Result of Distillation Time of Patchouli Leaf Dewaxing and Fermentation Sentot Joko Raharjo; Rurini Retnowati; Soebiantoro Soebiantoro
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (14.669 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.5

Abstract

The characteristic of patchouli oil of dewaxing, fermentation and time distillation toward patchouli leaves used GC-MS have been done.   The aim of the study was to characterization of patchouli oil on distillation time of patchouli leaves of dewaxing and fermentation.  The characteristics of patchouli oil on the distillation time for 12 hours, then the distillate collected every 2 hours showed that of the best result was the 3rd fraction collected distillate time of 12 hours with a yield of 0.56 %, light yellow color, specific gravity 0.9685 g/ mL and a refractive index of 1.5095 and patchouli alcohol of 69.56 %. Characteristics of patchouli oil on distillation time (2, 4, 6, 8, 10, 12) hours showed that of the best result was time distillation for 12 hours with a yield of 6.61 %, light yellow color, specific gravity of 0.9672 g/ mL, refractive index of 1.5082 and patchouli alcohol of 45.69 %. The other components of patchouli oil detected were alpha-gurjunene, cis-thujosene, beta-patchoulene, alpha-patchoulene, beta-caryophyllene, alpha-guaiene, seychellen, aromadendrene, beta-gurjunene, alpha-humulene, alpha-bulnesene, gemacrene-D, dehidroaromadendrene, gemacrene-A, gamma-patchoulene, valencene, viridiflorene, selina-3,7-(11)-dien, nor-patchoulenol, pogostol, illudol, globulol, beta-caryophyllen oksida, viridiflorol and ledol. Patchouli oil quality to meet requirements SNI 06-2385-2006 and ISO 3757:2002.
Efficacy of Mycoparasite Fecal Fungi Lecanicillium lecanii against Rust Disease (Phakopsora pachyrhizi) at Soybean (Glycine max L. Merril) Bintan R; Amin S. Leksono; Yusmani Prayogo
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (206.448 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.4

Abstract

The obstacle of efforts to increase the production of soybean is rust disease caused by obligate the parasite fungus Phakopsora pachyrhizi. One type of biological agent used to control rust disease is a mycoparasite fungus Lecanicillium lecanii. The aim of this study was to determine the effective density of conidia L. lecanii fungus for rust disease control and its impact on soybean yields. The study using complete randomized block design, three replication. The  treatment was the density of conidia L. lecanii i.e 104/mL, 105/mL, 106/mL, 107/mL, 108/mL and control. Isolates of the fungus L. lecanii was propagated on potato dextrose medium agar (PDA) in petri dishes. At the age of 21 days after inoculation conidia was taken one gram, then it was diluted with 10 ml sterile water and counted with a haemocytometer to obtain conidia density appropriate with the treatment. Furthermore, any suspension of conidia L. lecanii was applied to soybean at age 49-70 days after planting that was attacked by rust disease. Data were analyzed by ANOVA with SPSS 16.0 for windows. The analysis showed that treatment with a density of conidia 108/ml have better efficacy, as shown by the low intensity of the attacks by 15.9% compared to controls reached 27.15%. These results are also followed by a high number of fill-pods on the treatment 108/mL as much as 54.4 pods, dry seed weight of 9.85 grams, dry weight of 100 seeds 8.73 grams. Therefore that  L. lecanii fungus with density of conidia 108/mL can suppress the development of rust disease, can be used as a biological agent for substitute of chemical fungicides. 
Magnetic Levitation for Separation of Plastic Polyethylene Terephthalate (PET) and Polyvinyl Chloride (PVC) Gancang Saroja; Suyatman Suyatman; Nugraha Nugraha
Natural B, Journal of Health and Environmental Sciences Vol 1, No 4 (2012)
Publisher : Natural B, Journal of Health and Environmental Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (62.911 KB) | DOI: 10.21776/ub.natural-b.2012.001.04.6

Abstract

In the recycling of plastic waste, the process of plastic separation is often faced with the problem of the difficulty of separating PET from PVC plastics. The main objective of this research was to separate a mixed PET and PVC plastics using magnetic levitation. In the experiment, the samples were PET and PVC plastics from used bottles packaging found in the market. The magnetic field was derived from arrangement of the permanent magnets made from Neodymium with a cylinder shape with an orientation of magnetic moment parallel to its axis. Magnets were arranged so as to produce a magnetic field gradient in the vertical direction. The magnetic inductions at the respective surface of each magnet were 0.244, 0.349, 0.412, 0.443, 0.463, and 0.476 T. The paramagnetic fluid used was solution of MnCl2 with a concentration of 1, 1.5, 2, 2.5, and 3 M. The results showed that at Bo of 0,476 T, the fluid with the concentration of 3 M produced the highest levitation and the best separation of PET from PVC plastics.

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