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AGRIVITA, Journal of Agricultural Science
Published by Universitas Brawijaya
ISSN : 01260537     EISSN : 24778516     DOI : https://doi.org/10.17503
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research papers and article review in plant science i.e. agronomy, horticulture, plant breeding, soil sciences, plant protection and other pertinent field related to plant production. AGRIVITA is published three times per year. The Journal has been indexed in SCOPUS, Scimago Journal Ranks (SJR), Emerging Source Citation Index ( ESCI-Web of Science), EBSCO, ProQuest, Google Scholar and others international indexing. AGRIVITA is accredited first grade (Sinta 1/S1) for five years (2018-2023) based on Decree No: 30/E/KPT/2018 by Ministry of Research, Technology and Higher Education (Ristek Dikti), The Republic of Indonesia. We accept submission from all over the world. All submitted articles shall never been published elsewhere, original and not under consideration for other publication.
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Articles 20 Documents
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Search results for , issue "Vol 40, No 2 (2018): JUNE" : 20 Documents clear
Internal Versus Edge Row Comparison in Jajar Legowo 4:1 Rice Planting Pattern at Different Frequency of Fertilizer Applications Kartika, Kartika; Lakitan, Benyamin; Sanjaya, Nanda; Wijaya, Andi; Kadir, Sabaruddin; Kurnianingsih, Astuti; Widuri, Laily Ilman; Siaga, Erna; Meihana, Mei
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i0.1715

Abstract

Jajar legowo 4:1 cropping pattern has been adopted by rice farmers; however, there has been limited information on the comparison between internal and edge rows. In addition, the effects of timing and frequency of fertilizer applications on rice cultivated at riparian wetland also have to be understood. In this research, both single and split applications of fertilizer were employed. The single fertilizer applications were applied at 15 days after transplanting (DAT) (T1), 30 DAT (T2), 45 DAT (T3); and the split applications were 15+30 DAT (T4), 15+45 DAT (T5), 30+45 DAT (T6), and 15+30+45 DAT (T7). Results of this research indicated that crops in the edge rows produced higher leaf area index but those at internal rows produced higher dry weight biomass. Split fertilizer application to three times (T7) increased the weight of grains and number of filled spikelet but did not affect other shoot and root growth traits. Overall, fertilizer application increased leaf chlorophyll and nitrogen content. Jajar legowo 4:1 planting pattern and split fertilizer application to three times are recommended for increasing yield in rice cultivated at riparian wetlands.
Soil Biochemical Properties and Nutrient Leaching from Smallholder Oil Palm Plantations, Sumatra-Indonesia Kurniawan, Syahrul; Corre, Marife D.; Utami, Sri Rahayu; Veldkamp, Edzo
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1723

Abstract

The study aimed to assess soil biochemical properties and nutrient leaching in palm oil plantation. The research was conducted in smallholder oil palm plantations which were located in Jambi Province - Indonesia. Nutrient leaching was determined by measuring nutrient concentration in soil solution bi-weekly and monthly in the frond stacked and fertilized areas; soil water samples were collected by using suction cup lysimeter. The result showed that the application of mineral fertilizer (e.g. NPK) and dolomite resulted higher base saturation, exchangeable Ca, and available P in the fertilized than frond stacked and inter row areas (p ≤ 0.05). Stacking palm oil frond increased the soil macro-porosity, hence decreased leaching of K, Mg, Na, P, and total Al in the frond stacked than in the fertilized areas. The lower leaching losses and the higher soil macroporosity in the frond stacked than in the fertilized areas indicated that either the water did not dilute nutrient in the soil due to bypass flow, or the nutrient release from mineralization did not surpass nutrient demand which is quickly uptaken by palm root. Proper soil management through synchronizing rate of fertilizer application with nutrient output or frequency of fertilizer application may potentially minimize leaching losses.
Effects of Slope Position on Soil Physico-chemical Characteristics Under Oil Palm Plantation in Wet Tropical Area, West Sumatra Indonesia Yasin, Syafrimen; Yulnafatmawita, Yulnafatmawita
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.880

Abstract

This research was aimed to study soil physico-chemical properties at four slope positions under oil palm plantation in Dharmasraya, West Sumatra, Indonesia. Soils were sampled at 0-20 cm soil depth from 4 different slope positions (upper, middle, lower slope, and the bottom or flat area). The parameters analyzed were soil texture, SOM, bulk density, total pore, hydraulic conductivity, soil water potential (physical characteristics) as well as soil pH, CEC, Al-exchangeable, basic cations (Ca, Mg, K), N, and P (chemical characteristics). The results showed that the bottom area had better soil physicochemical properties than the others. SOM increased by 33%, total pore by 19%, void ratio by 47%, plant available water (PAW) by 28%, soil pH-H2O by 41%, CEC by 171 %, total-N by 170 %, and P-potential by 114 %, in contrast, soil BD and exchangeable-Al were lower (20 % and 96 %, respectively) in the bottom than in the sloping land. The middle slope had the poorest soil physico-chemical properties after 26 years of forest conversion into oil palm plantation. All sites had clay texture, the clay content increased (R2=0.93) by lowering slope position, and so did SOM content (R2=0.86), soil CEC (R2=0.93), and soil total-N values (R2=0.76).
Potential of Wood Vinegar for Enhancing Seed Germination of Three Upland Rice Varieties by Suppressing Malondialdehyde Production Atchima Dissatian; Jirawat Sanitchon; Paweena Pongdontri; Nuntawoot Jongrungklang; Darunee Jothityangkoon
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1332

Abstract

Upland rice usually gives poor germination under rainfed upland environement. To improve the seed germination, seed priming technique was assessed in this study. Seed of three upland rice (Oryza sativa L.) varieties (Leum Pua, ULR038 and Sakon Nakhon1, SKN) were primed with three different seed priming agents; distilled water, CaCl2 and 300-fold diluted wood vinegar. Compared to untreated dry seeds, wood vinegar improved field emergence and improved drought tolerance of the rice seeds better than other priming agents, which 50 % for Leum Pua, 20 % for ULR038 and 16% for SKN, when watering was delayed for 7 days. All three priming agents increased the field emergence percentage by similar amounts when watering was delayed for 14 days. To understand how priming helped improvement of germination, antioxidation mechanism and sugar metabolism were examined. Level of malondialdehyde was markedly reduced in all three rice varieties in response to priming, which were associated with increased activity of antioxidant enzymes, guaiacol peroxidase and ascorbate peroxidase. Wood vinegar also accelerated amylase activity in ULR038 and SKN, but not the sugar content. The results suggest that wood vinegar is a potent priming agent for achieving rapid and uniform seed germination in upland rice.
Gaseous, Physicochemical and Microbial Performances of Silicon Foliar Spraying Techniques on Cherry Tomatoes Mohammad Zahirul Islam; Mahmuda Akter Mele; Ho-Min Kang
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1792

Abstract

Silicon (Si) foliar spraying techniques (17 mM Si leaf and whole plant) were applied to determine the effect of gaseous, physicochemical and microbial activities on cherry tomatoes. Whole treated tomato plant showed significantly (p ≤ 0.05) the lowest respiration and ethylene production occurred during harvest time and after storage. The lowest fresh weight loss, fungal incidence and microbial activity were observed in whole plant treated tomatoes. In addition, the longest shelf life 24 days and the highest firmness were maintained by whole plant Si treated tomatoes. A higher vitamin C content was found in the whole Si treated tomato plants compared to Si leaf or no treated tomatoes. In addition, in the whole Si treated tomato plant showed the lowest soluble solid content by suppressing the color development. Based on the above results, whole plant Si treatment may be a useful technique to maintain respiration, ethylene production, firmness, shelf life and microbial activity of cherry tomatoes.
Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations Halimaton Saadiah Othman; Mohamad Osman; Zarina Zainuddin
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1365

Abstract

Stevia rebaudiana Bertoni originally from Paraguay belongs in Asteraceae family. It is an alternative source of non-caloric sweetener due to the sweet steviol glycosides contained in the leaves. As an introduced species in Malaysia, it is important to elucidate the genetic variabilities and relatedness among stevia accessions in order to broaden the genetic basis for future stevia breeding. This study described morphological and chemical variations and investigates genetic relationships among stevia accessions derived from across Malaysia and Paraguay using inter simple sequence repeats (ISSR) markers. HPLC (high-performance liquid chromatography) analysis also revealed high variability with stevioside content between 4.54 % (Taman Pertanian) to 20.36 % (Bangi) and rebaudioside A content varied between 0.3 % (Nilai) to 2.04 % (MNQ). From 32 ISSR markers, a total of 332 bands were scored, of which 264 (78 %) were polymorphic. The dendrogram from UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis separated 17 stevia accessions into 3 main groups. Rawang and Nilai were found to be closely related. The wide genetic variabilities among stevia accessions are a promising indicator towards the development of new stevia varieties. This valuable information will be able to assist parental selection in future stevia breeding programmes.
Transient Transformation of Potato Plant (Solanum tuberosum L.) Granola Cultivar Using Syringe Agroinfiltration Yesy John Mba’u; Iriawati Iriawati; Ahmad Faizal
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1467

Abstract

Genetic transformation has been used as an alternative approach to improve the quality and the productivity of potato plant. In this study, different conditions have been set up to optimize transient GFP (Green Fluorescence Protein) expression in potato cv. Granola. Leaves of potato were infiltrated with Agrobacterium tumefaciens strain C58C1 harboring pK7FWGF2 vector with a nuclear-targeted GFP by simple pressure. GFP signals allowed simple evaluation of transformation efficiency which were indicated by GFP expression in nucleus of leaf cells in infiltratedareas. The results showed that leaf position, co-cultivation time, optical density and the presence of acetosyringone significantly affected the transformation efficiency. The fourth terminal leaves from four-week old plants were the optimum age for transformation. Furthermore, the highest transient transformation efficiency was obtained upon 48 h post infiltration at an OD600 = 0.8, and the presence of 200 μM acetosyringone. In conclusion, the developed protocol will be useful to study gene function as well as to generate stable transformation of this potato cultivar.
Protoplast Fusion between Indonesian Citrus maxima (Burm.) Merr. and Citrus reticulata L.: A Preliminary Report Dyah Retno Wulandari; Agus Purwito; Slamet Susanto; Ali Husni; Tri Muji Ermayanti
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i0.950

Abstract

Protoplast fusion is a useful technique for citrus genetic improvement. Establishment of protoplast technology could produce triploid seedles citrus and new citrus genetic combination. The aim of this research was to establish protoplast isolation, protoplast fusion between pummelo ‘Nambangan’ (Citrus maxima) and keprok ‘Garut’ (C. reticulata), and its protoplast culture. Protoplasts were isolated from leaves of in vitro seedlings of pummelo ‘Nambangan’ and embryogenic callus of keprok ‘Garut’ with two different compositions of enzymes. The results showed that optimum enzymes compositions for cells mesophyl wall degradation were consisted of 0.5 % Onozuka cellulase RS10, 0.5 % Macerozyme R10 and 0.1 % Pectolyase Y23. Optimum enzymes composition for callus embryogenic cells wall degradation were 0.5 % Onozuka cellulase RS10 added with 0.5 % Macerozyme R10. Protoplast fusion was conducted with PEG-mediated. Protoplast mixture were incubated in 40 % PEG for 8-10 min and after that could be observed 40.6 % of unfused protoplasts, 4.6 % of homofused mesophyl protoplasts, 28.2 % of homofused callus protoplasts, 7.8 % of heterofused and 9.6 % of multifused protoplasts from mesophyl and callus. Cells divided at the first week and began to form cell colonies afterwards in liquid culture.
Effects of Sucrose and Plant Hormone on the Pigmentation of Mesocarp of White- and Red-Fleshed Peach Fruits Inna Martha Rumainum; Kanjana Worarad; Yoshikazu Yamaki; Kenji Yamane
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1094

Abstract

Three cultivars of peach (Prunuspersica L. Batsch): ‘Ikeda’, ‘Akatsuki’ (white-fleshed) and ‘Tenshin Suimitsuto’ (red-fleshed) were used to study the effect of sucrose and auxin on the pigmentation of mesocarp. In the first experiment, mesocarp discs of ‘Ikeda’ and ‘Tenshin Suimitsuto’ were incubated on solidified MS medium containing sucrose, 1-naphtalene acetic acid (NAA), and their combination. A treatment of 10μM NAA increased the total of anthocyanin content in ‘Ikeda’. In ‘Tenshin Suimitsuto’, a treatment of 100 mM sucrose increased the total of anthocyanin content. In the second experiment, mesocarp discs of ‘Akatsuki’ and ‘Tenshin Suimitsuto’ were employed to examine the effect of 2,3,5-triiodobenzoic acetic acid (TIBA). The mesocarp discs were incubated on solidified MS medium containing NAA or combination of NAA and TIBA. A single TIBA treatment was applied in ‘Tenshin Suimitsuto’. TIBA treatment significantly (P<0.05) reduced the total anthocyanin content in both cultivars. The total phenolic content was decreased by TIBA treatment in both cultivars. A treatment of single TIBA (200 mM) significantly (P<0.05) inhibited anthocyanin, flavonoid and phenolic accumulation in ‘Tenshin Suimitsuto’. Present study shows that sucrose and auxin might regulate anthocyanin synthesis, as well as phenolic compounds, in the flesh of peach fruit.
Micropropagation of Dendrobium phalaenopsis Orchid Through Overexpression of Embryo Gene AtRKD4 Setiari, Nintya; Purwantoro, Aziz; Moeljopawiro, Sukarti; Semiarti, Endang
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1690

Abstract

To increase the efficiency of crop production from Dendrobium phalaenopsis orchids, mass propagation has been performed by inducing somatic embryogenesis through Agrobacterium-mediated transformation of the Arabidopsis embryo gene AtRKD4 into orchid protocorm (developing orchid embryo). The three-week-old protocorms of D. phalaenopsis were genetically transformed with T-DNA carrying 35S :: GAL4 :: AtRKD4 :: GR through A. tumefaciens strain EHA 105. The cultures were maintained in VW medium with 10 mg L-1 Hygromycin. Due to the existence of glucocorticoid response element (GR) in the T-DNA construct, the transformed protocorms were transferred into VW medium with the addition of 15 μM Dexamethasone in 6 weeks after transformation to activate the transgene. A total of 12% protocorms has been confirmed for Hyg + by using PCR. The expression of embryo gene AtRKD4 was confirmed by cDNA analysis using AtRKD4 specific primers and Actin primers as a positive control experiment. The expression level of AtRKD4 in 2.5-month-old D. phalaenopsis transformant shoots was 7 times higher than non-transformant plants, and increased to 86 times higher in 8-months, that much higher than that of non-transformant. These results provide an improved method for genetic transformation of D. Phalaenopsis and will (eventually) increase production efficiency in the future.

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