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All Journal Prosiding Seminar Biologi AGRIVITA, Journal of Agricultural Science Indonesian Journal of Biotechnology Biosaintifika: Journal of Biology & Biology Education Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Sukarti Moeljopawiro
Faculty of Biology, Gadjah Mada University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology Materials Science & Nanotechnology

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Cytotoxicity of Buah Merah (Pandanus conoideus Lamk.) Extract on Breast Cancer Cell Line (T47D) Nuringtyas, Tri R; Pratama, Yoga; G, Galih; Wahyuono, Subagus; Moeljopawiro, Sukarti
Indonesian Journal of Biotechnology Vol 19, No 1 (2014)
Publisher : Universitas Gadjah Mada

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Abstract

Buah Merah (Pandanus conoideus Lamk.) has been extensively used to treat various diseases includingcancer. There are many varieties of buah merah and there was no scientifi c study comparing cytotoxicity ofdifferent varieties. The objective of this study was to investigate the cytotoxicity of three varieties of buah merahknown as Barugum, Maler and Yanggiru on breast cancer cell line (T47D). All samples were collected fromPapua, Indonesia. Each sample was extracted consecutively using three solvents chloroform, methanol andwater resulted to nine crude extracts. The cytotoxic activities were determined using MTT assay. The crudeextract showed the lowest IC50 was selected for further bioassay-guided fractionation. Fractionation was doneusing vacuum liquid chromatography coupled with preparative TLC to fi nd the active compounds. Severaldetection reagents were applied to TLC for identifi cation of the class of the potent compounds. The resultshowed that the potent extracts was obtained from Barugum methanol extract followed by Maler chloroformextract with IC50 value of 132.83 μg/ml and 139.72 μg/ml, respectively. All Yanggiru extracts did not showactivity. The bioassay-guided fractionation of Barugum and Maler extracts showed that the most potent fractioneluted by a mixture of hexane:ethyl acetate (75:25), was in Maler variety with IC50 value of 25,7 μg/ml, fourtimes higher than the most potent fraction of Barugum with IC50 value of 104,61 μg/ml. TLC analysis of themost potent fraction showed that the active compounds was class of terpene. Result of this study supportedthe utilization of buah merah Maler variety for breast cancer treatment.
Cloning of cDNA Encoding GRA1 Protein of Tachyzoite Toxoplasma Gondii Local Isolate Sulistyaningsih, Erma; Moeljopawiro, Sukarti; Subandono, Jarot; Artama, Wayan T.
Indonesian Journal of Biotechnology Vol 10, No 1 (2005)
Publisher : Universitas Gadjah Mada

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Gene encoding GRA1 protein is potent DNA-vaccine candidate against toxoplasmosis. The aim of the researchwas to clone the gene encoding GRA1 protein of tachyzoite Toxoplasma gondii local isolate by DNA recombinanttechnology. Tachyzoite was grown in Balb/c mice in vivo. Messenger RNA was isolated from total RNA and itwas used to synthesis cDNA. Complementary DNA encoding GRA1 protein of tachyzoite Toxoplasma gondii localisolate was amplified and cloned in a prokaryote cloning vector. The recombinant GRA1-encoding gene was thendigesting using EcoRI restriction endonuclease and sequencing. The result showed that the recombinant GRA1-encoding gene consisted of DNA sequences encoding all signal peptide and mature peptide of GRA1 protein.Alignment of recombinant GRA1 sequence to gene encoding GRA1 protein of Toxoplasma gondii RH isolate showed100% homologous.Keywords: GRA1 protein, Toxoplasma gondii, tachyzoite, cloning, cDNA
Flavonoid Production in Callus Cultures from Mesocarp of Stelechocarpus burahol Habibah, Noor Aini; Moeljopawiro, Sukarti; Dewi, Kumala; Indrianto, Ari
Biosaintifika: Journal of Biology & Biology Education Vol 8, No 2 (2016): September 2016
Publisher : Department of Biology, Faculty of Mathematics and Sciences, Semarang State University . Ro

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/biosaintifika.v8i2.6632

Abstract

Stelechocarpus burahol is one of the medicinal plants that contains flavonoids. The study was carried out to know flavonoid production of cultures in vitro S. burahol from mesocarp explants. Mesocarp explants were cultured on MS medium containing different combination and concentration of plant growth regulators i.e. picloram (5, 7.5 and 10 mg/L) and 2, 4-D (10, 15 and 20 mg/L) under dark condition. Induction of callus formation started on the 20.29th to the 29.86th days. Medium supplemented with Picloram and dark state proved to be the best condition for optimum callus induction from mesocarp explants of S. burahol. Callus grown on medium with the addition of 7.5 mg/l Picloram produces the highest flavonoid. The maximum production of the secondary metabolite was obtained from 8 weeks old callus. However, by the time of callus ageing, its output has declined. It could be concluded that callus cultures from mesocarp S. burahol can be used for flavonoid production.How to CiteHabibah, N. A., Moeljopawiro, S. Dewi, K. & Indrianto, A. (2016). Flavonoid Production in Callus Cultures from Mesocarp ofStelechocarpus burahol. Biosaintifika: Journal of Biology & Biology Education, 8(2), 214-221.
Micropropagation of Dendrobium phalaenopsis Orchid Through Overexpression of Embryo Gene AtRKD4 Setiari, Nintya; Purwantoro, Aziz; Moeljopawiro, Sukarti; Semiarti, Endang
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.17503/agrivita.v40i2.1690

Abstract

To increase the efficiency of crop production from Dendrobium phalaenopsis orchids, mass propagation has been performed by inducing somatic embryogenesis through Agrobacterium-mediated transformation of the Arabidopsis embryo gene AtRKD4 into orchid protocorm (developing orchid embryo). The three-week-old protocorms of D. phalaenopsis were genetically transformed with T-DNA carrying 35S :: GAL4 :: AtRKD4 :: GR through A. tumefaciens strain EHA 105. The cultures were maintained in VW medium with 10 mg L-1 Hygromycin. Due to the existence of glucocorticoid response element (GR) in the T-DNA construct, the transformed protocorms were transferred into VW medium with the addition of 15 μM Dexamethasone in 6 weeks after transformation to activate the transgene. A total of 12% protocorms has been confirmed for Hyg + by using PCR. The expression of embryo gene AtRKD4 was confirmed by cDNA analysis using AtRKD4 specific primers and Actin primers as a positive control experiment. The expression level of AtRKD4 in 2.5-month-old D. phalaenopsis transformant shoots was 7 times higher than non-transformant plants, and increased to 86 times higher in 8-months, that much higher than that of non-transformant. These results provide an improved method for genetic transformation of D. Phalaenopsis and will (eventually) increase production efficiency in the future.
OPTIMASI KONDISI FERMENTASI UNTUK PRODUKSI SELULOSA BAKTERI OLEH STRAIN SLK-1 DALAM MEDIA DASAR AIR KELAPA (OPTIMIZATION OF FERMENTATION CONDITIONS FOR THE PRODUCTION OF BACTERIAL CELLULOSE BY SLK-1 STRAIN IN COCONUT WATER BASED MEDIUM) Sarkono, Sarkono; Moeljopawiro, Sukarti; Setiaji, Bambang; Sembiring, Langkah
Prosiding Seminar Biologi Vol 9, No 1 (2012): Seminar Nasional IX Pendidikan Biologi
Publisher : Prodi Pendidikan Biologi FKIP UNS

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Abstract

ABSTRAK   Tujuan penelitian ini adalah mengoptimasi kondisi fermentasi terbaik strain Bakteri Asam Asetat penghasil selulosa yaitu isolat SLK-1.  Strain ini diisolasi dari buah salak pada penelitian sebelumnya.  Hasil optimasi menunjukkan bahwa kondisi fermentasi optimum untuk pertumbuhan dan produksi selulosa pada isolat SLK-1  dicapai dengan sumber karbon gula pasir, sumber nitrogen ammonium sulfat, pH 7, suhu inkubasi 25°C dan metode fermentasi statis. Karakter struktur permukaan selulosa hasil fermentasi isolat SLK-1 dipengaruhi oleh metode fermentasi yang digunakan.  Metode fermentasi goyangan berpengaruh menurunkan produksi selulosa pada  isolat SLK-1 dan merubah struktur permukaan yaitu susunan mikrofibril lebih renggang dan membentuk gelembung.   Kata Kunci: bakteri asam asetat, optimasi, fermentasi, selulosa bakteri, penggoyangan
Isolasi dan Uji Aktivitas Antibakteri Actinomycetes dari Rhizosfer Bakau di Hutan Bakau Torosiaje Gorontalo Retnowati, Yuliana; Sembiring, Langkah; Moeljopawiro, Sukarti; Djohan, Tjut S.; Soetarto, Endang S.
Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek) 2017: Prosiding SNPBS (Seminar Nasional Pendidikan Biologi dan Saintek)
Publisher : Universitas Muhammadiyah Surakarta

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Abstract

Actinomycetes penghasil antibiotik telah dieksplorasi dari berbagai sumber di lingkungan, terutama lingkungan ekstrim.Hutan bakau Torosiaje di Provinsi Gorontalo memiliki kondisi geomorfologi yang unik berupa ekosistem hutan bakau karsdengan dua tipe area yaitu tipe fringe dan overwash mangrove yang tersusun oleh jenis bakau yang bervariasi. Penelitian inidi desain untuk mendapatkan isolat Actinomycetes dari rhizosfer berbagai jenis bakau di hutan bakau Torosiaje Gorontalodan menganalisis aktifitas antibakteri melawan bakteri patogen. Sampel tanah dikoleksi dari rizosfer tujuh jenis pohon bakauyaitu Rhizophora mucronata dan Bruguiera gymnorhiza pada tipe hutan overwash, Rhizophora apiculata, Bruguieragymnorhiza pada zona middle tipe hutan Fringe, Avicenia marina, Xylocarpus sp, Ceriops tagal dan Soneratia alba padazona upper tipe hutan fringe. Pre-treatmen sampel tanah berdasarkan metode panas basah pada suhu 60oC selama 15 menit.Isolasi selektif Actinomycetes menggunakan medium Starch Casein Agar yang disuplementasi dengan cyclohexamide dannystatin. Seleksi isolat penghasil antibiotik berdasarkan metode agar blok menggunakan bakteri uji Eschericia coli,Staphylococcus aureus dan Bacillus subtillis. Aktifitas antibakteri ditandai dengan pembentukan zona hambat disekitarpertumbuhan actinomyctes. Diameter zona hambat dan diameter koloni Actinomycetes diukur untuk menentukan indekszona hambat. Hasil penelitian diperoleh sebanyak 167 isolat Actinomycetes yang terdistribusi pada rizosfer 7 jenis bakau. 77isolat Actinomycetes menunjukkan aktifitas antibakteri melawan bakteri patogen, terdiri dari 52 isolat melawan bakteriGram-positif (narraw spectrum) dan 25 isolat melawan bakteri Gram-positif dan Gram-negatif (broad spectrum). IsolatActinomycetes penghasil antibiotik memiliki karakter morfologi yang bervariasi yang didominasi oleh koloni berwana putihdan pigmen terdifusi berwarna kekuningan sampai coklat dan dikelompokkan kedalam 15 grup.
Co-Authors . HERNAYANTI Ahmad Hamim Sadewa Ari Indrianto Aziz Purwantoro Bambang Hariono Bambang Setiaji ENDANG S. SOETARTO Endang Semiarti Erma Sulistyaningsih Galih G, Galih Ida Ayu Putu Sri Widnyani Jarot Subandono Kumala Dewi LANGKAH SEMBIRING Nintya Setiari Noor Aini Habibah Subagus Wahyuono Tjut S. Djohan Tri R Nuringtyas, Tri R Wayan T. Artama Yuliana Retnowati