AGRIVITA, Journal of Agricultural Science
AGRIVITA Journal of Agricultural Science is a peer-reviewed, scientific journal published by Faculty of Agriculture Universitas Brawijaya Indonesia in collaboration with Indonesian Agronomy Association (PERAGI). The aims of the journal are to publish and disseminate high quality, original research papers and article review in plant science i.e. agronomy, horticulture, plant breeding, soil sciences, plant protection and other pertinent field related to plant production. AGRIVITA is published three times per year. The Journal has been indexed in SCOPUS, Scimago Journal Ranks (SJR), Emerging Source Citation Index ( ESCI-Web of Science), EBSCO, ProQuest, Google Scholar and others international indexing. AGRIVITA is accredited first grade (Sinta 1/S1) for five years (2018-2023) based on Decree No: 30/E/KPT/2018 by Ministry of Research, Technology and Higher Education (Ristek Dikti), The Republic of Indonesia. We accept submission from all over the world. All submitted articles shall never been published elsewhere, original and not under consideration for other publication.
Articles
934 Documents
Analysis of Variance, Heritability, Correlation and Selection Character of M1 V3 Generation Cassava (Manihot esculenta Crantz) Mutants
Rahmi Henda Yani;
Nurul Khumaida;
Sintho Wahyuning Ardie;
Muhamad Syukur
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v40i1.844
Information about genetic variability and correlation between qualitative character and yield are important to support a selection program. The objective of this research was to determine genetic variability, heritability, and path analysis of M1 V3 cassava mutants’ characters. This research was conducted at Bogor Agricultural University Experimental Field Research from May 2014 to May 2015. This research used 32 mutants from five cassava parent lines which were Malang-4 and Adira-4 (national varieties), UJ-5 (Introduction variety from Thailand), and two local genotypes from Halmahera which were Jame-jame and Ratim. The results showed that gamma ray irradiation increased variability from five cassava genotypes. Characters that had high heritability were length of leaf lobe, lengthof petiole, stem diameter, and the height of plant. The path correlation analysis showed that number of tubers, number of economic tuber (> 20 cm), height to first branchingand stem diameter had direct correlation with tuber mass per plant. The characters can be used for the selection of M1 V4 generation.
Development and Survivorship of Scirtothrips dorsalis Hood (Thysanoptera: Thripidae) in Different Growth Stages of Mango and Selected Weeds
Affandi, Affandi;
dela Rosa Medina, Celia;
Velasco, Luis Rey Ibanez;
Javier, Pio Arestado;
Depositario, Dinah Pura Tonelete
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v40i0.1650
The research objective was to quantify the development and survivorship rate of S. dorsalis in different phenological stages of mango and selected weeds. The research was conducted in the laboratory of PT. Trigatra Rajasa, Mango plantation in Ketowan, Arjasa, Situbondo, East Java, Indonesia from February to September 2015. The development and survivorship rate were done through observation of life span of S. dorsalis from egg to pupa. Analysis of Variance and Duncan Multiple Range Test (p = 0.05) with 5 replications were applied to ensure the significant differences among the treatments. The result showed that development and survivorship of Scirtothrips dorsalis were supported by mango flushes and flower as well as some weeds such as Leucania leucochepala, Ipomoea triloba, Achalypha indica, Desmanthus leptophyllus and Azadirachta indica as source of food. Achalypha indica was the most suitable host with development time (12.82 ± 0.21 days) and survivorship (33 %). Weed Tridax procumbent, Momordica charantia and Mimosa pudica were unable to provide the living requirement for immature developmental stage of S. dorsalis.
Genetic Variability, Standardized Multiple Linear Regression and Principal Component Analysis to Determine Some Important Sesame Yield Components
Vina Eka Aristya;
Taryono Taryono;
Rani Agustina Wulandari
AGRIVITA, Journal of Agricultural Science Vol 39, No 1 (2017): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v39i1.843
Sesame is an important commodity in supporting various industries such as low saturated fat oil producing and are often able to adapt under stressed grown conditions. Breeding sesame is undertaken to increase production and is possible by radiation induced polygenic characteristic changes with a gamma rays source. The study aims to identify the effectiveness of genetic variability, standardized multiple linear regression, and principal component analysis to determine some important sesame yield components for indirect selection. Eighteen sesame mutant lines (black and white types) were studied for eleven quantitative traits. Two sesame types were irradiated with eight doses (100-800 Gy) of gamma rays individually. Variability studies on seed yield and yield components are important raw material of high productivity for all studied traits. Standardized multiple linear regression analysis is the most effective way to provide information of relationship between seed yield and yield components in sesame mutant lines for indirect selection.
Genotypic Variability in Carbon Isotope Discrimination and Water Use Efficiency among Recombinant Inbred Lines of Sunflower (Helianthus annuus L.)
Afifuddin Latif Adiredjo;
Philippe Grieu
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v40i0.1728
To evaluate genotypic variability in carbon isotope discrimination or CID and water use efficiency or WUE, recombinant inbred lines (RILs) population of sunflower (Helianthus annuus L.) was used. Seventy eight sunflower RILs were grown in greenhouse and 100 sunflower RILs were grown under field condition, and measured some morphological and physiological traits including leaf area at flowering (LAf), net CO2 assimilation rate (A) and transpiration per day at flowering (Ef). WUE, called “potential” WUE (WUEp), was calculated as the ratio of assimilation potential (Ap) to transpiration per day at flowering (Ef) where Ap was derived from the multiplication of A with LAf. The CID was significantly varied among RILs and there was significant negative genetic correlation between CID and WUEp. Heritability of the CID was higher rather than the WUEp which reflected wide genetic variability of CID. The genetic correlation between CID and WUEp and the wide genotypic variability of CID indicated that CID can be proposed as an indicator to determine WUE in sunflower and open a way in understanding the genetic diversity of the RILs which could be used as a basic consideration before applying selection program in sunflower breeding.
Selection Strategy of Drought Tolerance of Red Rice Mutant Lines
Eries D. Mustikarini;
Noer Rahmi Ardiarini;
Nur Basuki;
Kuswanto Kuswanto
AGRIVITA, Journal of Agricultural Science Vol 39, No 1 (2017): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v39i1.648
Most of Indonesian red rice is not grown in dry land. New varieties could be breeded through mutation breeding. This research objective was to evaluate the selection strategy of drought tolerant on red rice. The study was conducted on ultisol soil in Bangka Belitung Province, Indonesia, in February 2012-February 2015. It consisted of three activities, selection M5, evaluation of selected M5 and evaluation of drought critical period on selected M6. Mutant of M5 was selected and evaluated with polyethylene glycol (PEG) -0.5 MPa and less of soil moisture. Selected M6 was evaluated to observe a critical period of drought stress. Evaluation with PEG produces five superior mutants that more vigor than the elders. Selection on low humidity shows that M5-GR150-1-9 produces higher filled grain and weight filled grain than other mutants and elders. The selected red rice line M5-GR150-1-9-13, has a better tolerance towards drought than its control. It could be obtained such mutant lines which have a high yield, early maturing and drought tolerant from the result of six generations gamma irradiation mutant selection.
Gaseous, Physicochemical and Microbial Performances of Silicon Foliar Spraying Techniques on Cherry Tomatoes
Mohammad Zahirul Islam;
Mahmuda Akter Mele;
Ho-Min Kang
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v40i2.1792
Silicon (Si) foliar spraying techniques (17 mM Si leaf and whole plant) were applied to determine the effect of gaseous, physicochemical and microbial activities on cherry tomatoes. Whole treated tomato plant showed significantly (p ≤ 0.05) the lowest respiration and ethylene production occurred during harvest time and after storage. The lowest fresh weight loss, fungal incidence and microbial activity were observed in whole plant treated tomatoes. In addition, the longest shelf life 24 days and the highest firmness were maintained by whole plant Si treated tomatoes. A higher vitamin C content was found in the whole Si treated tomato plants compared to Si leaf or no treated tomatoes. In addition, in the whole Si treated tomato plant showed the lowest soluble solid content by suppressing the color development. Based on the above results, whole plant Si treatment may be a useful technique to maintain respiration, ethylene production, firmness, shelf life and microbial activity of cherry tomatoes.
Genetic Variabilities of Stevia rebaudiana Bertoni Cultivated in Malaysia as Revealed by Morphological, Chemical and Molecular Characterisations
Halimaton Saadiah Othman;
Mohamad Osman;
Zarina Zainuddin
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v40i2.1365
Stevia rebaudiana Bertoni originally from Paraguay belongs in Asteraceae family. It is an alternative source of non-caloric sweetener due to the sweet steviol glycosides contained in the leaves. As an introduced species in Malaysia, it is important to elucidate the genetic variabilities and relatedness among stevia accessions in order to broaden the genetic basis for future stevia breeding. This study described morphological and chemical variations and investigates genetic relationships among stevia accessions derived from across Malaysia and Paraguay using inter simple sequence repeats (ISSR) markers. HPLC (high-performance liquid chromatography) analysis also revealed high variability with stevioside content between 4.54 % (Taman Pertanian) to 20.36 % (Bangi) and rebaudioside A content varied between 0.3 % (Nilai) to 2.04 % (MNQ). From 32 ISSR markers, a total of 332 bands were scored, of which 264 (78 %) were polymorphic. The dendrogram from UPGMA (Unweighted Pair Group Method with Arithmetic Mean) cluster analysis separated 17 stevia accessions into 3 main groups. Rawang and Nilai were found to be closely related. The wide genetic variabilities among stevia accessions are a promising indicator towards the development of new stevia varieties. This valuable information will be able to assist parental selection in future stevia breeding programmes.
Genetics Analysis and Heritability of Fruit Characters in Muskmelon (Cucumis melo L.) Using Extreme Parental Differences
Sakulphrom, Sunisa;
Chankaew, Sompong;
Sanitchon, Jirawat
AGRIVITA, Journal of Agricultural Science Vol 40, No 1 (2018): FEBRUARY
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v40i1.1133
Taeng-Thai is the Thai name for oriental pickling melon or muskmelon (Cucumis melo L.) which is a Cucumis species. Based on consumption as Thai traditional dessert, fruit characters at ripening stage are very important to improve the muskmelon cultivar. Understanding the genetic control of fruit traits is the key to a successful for crop improvement. Research aimed to determine the genetic effects, number of genes and heritability of fruit characters in muskmelon. The results have shown that both additive and dominant effects governed for fruit weight, fruit length, fruit width and fruit thickness with the number of gene as 40.26, 1.55, 10.70 and 11.10 genes, respectively. Simple phenotypic correlation coefficients shown, had a significant positive correlation among the four fruit traits. Due to the quantitative inheritance of fruit characters, quantitative traits loci (QTL) mapping of those traits were necessary to identify all controlling genes of fruit traits in further muskmelon improvement.
Transient Transformation of Potato Plant (Solanum tuberosum L.) Granola Cultivar Using Syringe Agroinfiltration
Yesy John Mba’u;
Iriawati Iriawati;
Ahmad Faizal
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v40i2.1467
Genetic transformation has been used as an alternative approach to improve the quality and the productivity of potato plant. In this study, different conditions have been set up to optimize transient GFP (Green Fluorescence Protein) expression in potato cv. Granola. Leaves of potato were infiltrated with Agrobacterium tumefaciens strain C58C1 harboring pK7FWGF2 vector with a nuclear-targeted GFP by simple pressure. GFP signals allowed simple evaluation of transformation efficiency which were indicated by GFP expression in nucleus of leaf cells in infiltratedareas. The results showed that leaf position, co-cultivation time, optical density and the presence of acetosyringone significantly affected the transformation efficiency. The fourth terminal leaves from four-week old plants were the optimum age for transformation. Furthermore, the highest transient transformation efficiency was obtained upon 48 h post infiltration at an OD600 = 0.8, and the presence of 200 μM acetosyringone. In conclusion, the developed protocol will be useful to study gene function as well as to generate stable transformation of this potato cultivar.
Protoplast Fusion between Indonesian Citrus maxima (Burm.) Merr. and Citrus reticulata L.: A Preliminary Report
Dyah Retno Wulandari;
Agus Purwito;
Slamet Susanto;
Ali Husni;
Tri Muji Ermayanti
AGRIVITA, Journal of Agricultural Science Vol 40, No 2 (2018): JUNE
Publisher : Faculty of Agriculture University of Brawijaya in collaboration with PERAGI
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DOI: 10.17503/agrivita.v40i0.950
Protoplast fusion is a useful technique for citrus genetic improvement. Establishment of protoplast technology could produce triploid seedles citrus and new citrus genetic combination. The aim of this research was to establish protoplast isolation, protoplast fusion between pummelo ‘Nambangan’ (Citrus maxima) and keprok ‘Garut’ (C. reticulata), and its protoplast culture. Protoplasts were isolated from leaves of in vitro seedlings of pummelo ‘Nambangan’ and embryogenic callus of keprok ‘Garut’ with two different compositions of enzymes. The results showed that optimum enzymes compositions for cells mesophyl wall degradation were consisted of 0.5 % Onozuka cellulase RS10, 0.5 % Macerozyme R10 and 0.1 % Pectolyase Y23. Optimum enzymes composition for callus embryogenic cells wall degradation were 0.5 % Onozuka cellulase RS10 added with 0.5 % Macerozyme R10. Protoplast fusion was conducted with PEG-mediated. Protoplast mixture were incubated in 40 % PEG for 8-10 min and after that could be observed 40.6 % of unfused protoplasts, 4.6 % of homofused mesophyl protoplasts, 28.2 % of homofused callus protoplasts, 7.8 % of heterofused and 9.6 % of multifused protoplasts from mesophyl and callus. Cells divided at the first week and began to form cell colonies afterwards in liquid culture.