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Journal of Mathematical and Fundamental Sciences
Published by Institut Teknologi Bandung
ISSN : 23375760     EISSN : 23385510     DOI : https://doi.org/10.5614/j.math.fund.sci.
Core Subject : Science, Education,
Astronomy Chemistry Earth & Planetary Sciences Mathematics Physics
Journal of Mathematical and Fundamental Sciences welcomes full research articles in the area of Mathematics and Natural Sciences from the following subject areas: Astronomy, Chemistry, Earth Sciences (Geodesy, Geology, Geophysics, Oceanography, Meteorology), Life Sciences (Agriculture, Biochemistry, Biology, Health, Medical, Pharmacy), Mathematics, Physics, and Statistics.
Arjuna Subject : Matematika - Matematika (Lain-Lain)
Articles 5 Documents
 1 
Search results for , issue "Vol. 54 No. 3 (2023)" : 5 Documents clear
Synthesis of Human Antibodies Against HBsAg in Newly Established Chinese Hamster Lung (CHL-YN) Cell Line Meutia Diva Hakim; Noriko Yamano- Adachi; Takeshi Omasa; Marselina Irasonia Tan; Lia Dewi Juliawaty; Ernawati Arifin Giri-Rachman
Journal of Mathematical and Fundamental Sciences Vol. 54 No. 3 (2023)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2023.54.3.1

Abstract

Hepatitis B immunoglobulin (HBIG) is an effective treatment for hepatitis B, including postexposure prophylaxis of HBV infection, prevention of HBV reinfection in liver transplant patients, and reducing sexual transmission. This study investigated the synthesis of human IgG antibodies that specifically target HBsAg subtype adr in CHL-YN cells, a newly established cell line that grows faster than CHO-K1. To achieve the synthesis of human IgG antibodies, a plasmid vector encoding DNA sequences for human IgG antibodies against HBsAg was constructed and then transiently transfected into CHL-YN cells. The expression and antigen-binding capacity of the recombinant human IgG antibodies were analyzed using western blot and ELISA. The results showed successful expression and secretion of human IgG antibodies that recognize HBsAg subtype adr in CHL-YN cells. The ELISA test confirmed the specificity of the human IgG antibodies towards HBsAg subtype adr. Thus, this study concluded that human IgG antibodies that target HBsAg subtype adr were transiently expressed in CHL-YN cells.
Evaluation NO2 Detection Using Low-Cost Folded-Path Photometer Januar Arif Fatkhurrahman; Puji Lestari
Journal of Mathematical and Fundamental Sciences Vol. 54 No. 3 (2023)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2023.54.3.5

Abstract

The measurement of nitrogen dioxide (NO2) in industrial and residential areas needs comprehensive and reliable instrumentation providing long-interference-free operation and minimum maintenance and recalibration. Differential optical absorption spectroscopy can be used as a direct measurement technique based on the specific absorption characteristics of NO2 following the Berr-Lambert law. This paper proposes a low-cost folded-path photometer to measure NO2 in the air. Cheap tubular acrylic was used as a detection cell with a 3D printed framework, making it compact, modular, and flexible. Evaluation of this differential optical absorption spectroscope (DOAS) was conducted by instrument test responses using NO2 gas. The estimated LOD was ~1263 ppb using a 2-nm resolution of the spectrometer and a 6-meter detection cell length. Deviation of the DOAS was estimated to be 0.8% at high concentration and 2.85% at low concentration based on the calibrated DOAS. Intercomparison of the results was conducted using two different instruments to evaluate the DOAS’s performance by measuring NO2 from motorcycle emissions, which indicated that there was a good correlation between the results. The coefficient correlation (R) was 0.649 for the DOAS- ASTM D1607 Griesz Saltzmann method pairing and 0.846 for the DOAS- electrochemical gas analyzer pairing.
Applying Fluorescence Dissolved Organic Matter Spectra and Phytoplankton Biology Index for Assessing Urban River Quality Okik Hendriyanto Cahyonugroho; Sucipto Hariyanto; Ganden Supriyanto
Journal of Mathematical and Fundamental Sciences Vol. 54 No. 3 (2023)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2023.54.3.3

Abstract

The relationship between phytoplankton dynamics and dissolved organic matter (DOM) in aquatic systems based on fluorophore organic matter properties results in poor implementation of methods for water quality monitoring. This study aimed to investigate the relation between fluorophore organic matter and phytoplankton index and to estimate the phytoplankton index by fluorescent organic components for water quality assessment. The research was conducted by sampling urban river water, followed by measuring the biological phytoplankton index and dissolved organic matter during the wet and dry seasons. Fluorescence Excitation Emission Matrices (FEEMs) analysis combined with Parallel Factor (PARAFAC) analysis identified three main components of the fluorescence organic matter, i.e., a fulvic acid-like component (C1) and humic acid-like components (C2 and C3), in the aquatic system, whether in the dry season or the wet season. C1 was mainly generated by phytoplankton species, i.e., Nitzschia sp., Plectonema sp., Navicula sp., and Pinnularia sp., and C2 and C3 had a less significant relationship with phytoplankton species. The diversity of Bacillariophyta had a stronger contribution to the release of DOM than the diversity of Cyanobacteria. The abundance index of a particular phytoplankton species can be used to determine the organic components of FEEM-derived fluorescence organic matter.
Havanensin-type Limonoids from the Stem Bark of Chisocheton pentandrus (Meliaceae) Dudi Runadi; Purnama Purnama; Supriatno Salam; Nulelasari Nurlelasari; Desi Harneti; Tri Mayanti; Rani Maharani; Unang Supratman; Yoshihito Shiono
Journal of Mathematical and Fundamental Sciences Vol. 54 No. 3 (2023)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2023.54.3.2

Abstract

A new limonoid, belonging to the havanensin-type, called pentandricine E (1), alongside four other known types, i.e., trichilenone acetate (2), toonaciliatones C (3), 11α-acetoxyazadirone (4) and ceramicine I (5), were found in Chisocheton pentandrus stem bark. The structures of the isolated 1-5 were identified by NMR spectroscopy and HRTOFMS. Pentandricine E (1) displayed the highest cytotoxicity toward the MCF-7 breast cancer line with an IC50 value of 63.27 ± 0.21 mM.
Shallow S-wave Velocity Profile Estimation using Surface Velocity and Microtremor HVSR with a Linear Velocity Increase Approach Andi Muhamad Pramatadie; Hiroaki Yamanaka; Afnimar Afnimar
Journal of Mathematical and Fundamental Sciences Vol. 54 No. 3 (2023)
Publisher : Directorate for Research and Community Services (LPPM) ITB

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.5614/j.math.fund.sci.2023.54.3.4

Abstract

We propose a simple method for 1D S-wave velocity (Vs) profile estimation using a measured surface S-wave velocity (V1) and peak frequency of the observed microtremor horizontal-to-vertical spectral ratio (HVSR). In this method, the S-wave velocity profile is presented as linear velocity increase with depth in sediments over a bedrock layer that has a given constant S-wave velocity. Thus, the profile can be parameterized with the measured surface S-wave velocity and the velocity gradient. The gradient can be estimated based on the agreement of the peak frequencies of the observed microtremor HVSR and the theoretical ellipticity of the fundamental mode of the Rayleigh wave. We examined the applicability of the proposed method using numerical tests as well as application to actual data at five sites in the Bandung Basin, Indonesia, where observed Rayleigh wave phase velocities from microtremor array surveys were available. The applicability was confirmed in numerical tests using sample models of soil profiles in the basin. Actual application indicated the appropriateness of the estimated S-wave velocity profiles due to the similarity of their theoretical Rayleigh wave phase velocities with the observed Rayleigh wave phase velocities. Since the proposed method needs prior confirmation of the linear increase of the S-wave velocity, it is suitable for use in spatial interpolation of shallow S-wave velocity profiles with simple data acquisition.

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