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Samsul Kamal
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Prodi Pendidikan Biologi Gedung B Lantai 1 Fakultas arbiyah dan Keguruan UIN Ar-Raniry, Jl. Syeh Abdul Rauf Kopelma Darussalam, Kode pos 23111
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BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan
Published by Universitas Islam Negeri Ar-Raniry Banda Aceh
ISSN : 23379812     EISSN : 25491768     DOI : http://dx.doi.org/10.22373/biotik
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Education
BIOTIK: Jurnal Ilmiah Biologi Teknologi dan Kependidikan publishes scientific articles in the field of biology education and technology. Furthermore, this journal bridges the gap between research and practice, providing information, ideas and insights, in addition to critical examinations of biology research and teaching. Through the coverage of policy and curriculum developments, the latest results of research into the teaching, learning and assessment of biology are brought to the fore. This journal is published by the Department of Biology Education in cooperation with the Center for Research and Community Service (LP2M) Universitas Islam Negeri Ar-Raniry Banda Aceh, Aceh, Indonesia.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 322 Documents
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Development of Herbal Booster Product “A5I (Katuk (S. androgynus (L.) Merr.), Moringa (M. oleifera), Fenugreek (T. foenum-graecum L.), Torch Ginger (Etlingera elatior), Cinnamon (Cinnamomum burmannii))” as a Lactagogue Based on Clinical Observational Study Eva Kusumahati; Kintoro Kintoro; Sagiran Sagiran
Biotik Vol 14 No 1 (2026): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v14i1.34463

Abstract

Inadequate exclusive A5I provision is a risk factor for stunting, which remains a major health problem in Indonesia. A polyherbal tea product A5I containing katuk (Sauropus androgynus), moringa (Moringa oleifera), klabet (Trigonella foenum-graecum), torch ginger (Etlingera elatior), and cinnamon (Cinnamomum burmannii) was developed as a natural lactagogum. This study aimed to evaluate the safety of the product through histopathological analysis of vital organs and mammary tissue in female mice after repeated administration. A 28-day subchronic toxicity test was conducted on female mice divided into control and treatment groups. The heart, lungs, kidneys, and mammary organs were harvested for histopathological examination using hematoxylin-eosin staining with a standardized scoring system. Myocardial histopathology showed minimal bleeding without necrosis. The lungs exhibited alveolar septal destruction and mild inflammatory cell infiltration without pulmonary edema, indicating a functional alveocapillary barrier. The kidneys exhibited only mild tubular degeneration without necrosis or inflammatory infiltration. Mammary tissue exhibited extensive ductal dilation and abundant intraluminal secretion distribution, consistent with active lactation. Subchronic administration of A5I polyherbal tea did not cause significant histopathological damage to vital organs, and the mammary findings support its potential lactagogic effect. These findings provide preliminary evidence of the safety of this polyherbal product as a breast milk booster, although further studies with larger sample sizes are needed to confirm its safety and clinical effectiveness. Keyword: Breastfeeding Booster; Histopathology; Lactagogue; Mammary Gland; Mice; Polyherbal
Experimental Study on Delayed Cell Death After Thawing and the Effect of Cryoprotective Base Solutions Ho, YongIl; Mun, JongHyong; Pyo, KumChon; Pak, NamIl; Choi, SongGuk; Kang, Chol; Li, Chol; Lim , GyongMan; Choi, YongDo
Biotik Vol 14 No 1 (2026): JURNAL BIOTIK
Publisher : Universitas Islam Negeri Ar-Raniry

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/biotik.v14i1.33377

Abstract

BACKGROUND: Cryopreservation is a cornerstone technology in regenerative medicine, cell therapy, and biobanking; however, delayed cell death (DCD) occurring 24–48 hours post-thaw remains a critical barrier to maintaining high-quality viable cells—even when immediate post-thaw viability exceeds 95%.  OBJECTIVE:   To investigate the occurrence of delayed cell death (DCD) in cells after thawing following cryopreservation, and to evaluate the regulatory effect of different cryoprotective base solutions, with a focus on establishing a UW solution-based cryopreservation protocol.  MATERIALS AND METHODS: Six cell lines (PESUN, Vero, RD, RK13, VeroE6, L20B) were used to compare two freezing methods (rapid freezing vs. slow freezing) and two cryoprotective base solutions (DMEM vs. UW solution). Cell viability was detected by trypan blue staining, apoptotic cells by Giemsa staining, and cell biological characteristics (proliferation, viral susceptibility) were assessed.  RESULTS: 1) DCD peaked at 24 h post-thaw: the mortality rate was 42.2% and apoptotic rate was 28.9% in DMEM-based groups. 2) UW solution (intracellular-type, low Na⁺/high K⁺) significantly reduced DCD: 24 h post-thaw mortality was 21.8% (vs. 43.2% in DMEM) and apoptotic rate was 13.9% (vs. 28.9% in DMEM, both P<0.05). 3) The optimal UW-based protocol was confirmed as: 20% dimethyl sulfoxide (DMSO), 1 min room temperature equilibration, rapid freezing, 37°C water bath thawing, 2.5% glucose in dilution medium, and ≥4-fold dilution. 4) Cells preserved by this protocol maintained pre-cryopreservation properties (proliferation index: 3.27 vs. 3.23; doubling time: 41.8 h vs. 42.2 h; Echovirus B1 log₁₀ TCID₅₀/mL: 7.50 vs. 7.50).  CONCLUSION: Post-thaw DCD is closely associated with apoptosis. The UW solution-based cryopreservation protocol effectively reduces DCD while preserving cell function, providing a reliable strategy for clinical cell cryopreservation.

Page 33 of 33 | Total Record : 322

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