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agriTECH

agritech
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Published by Universitas Gadjah Mada

ISSN : 02160455 EISSN : 25273825 DOI : 10.22146/agritech

Core Subject : Agriculture,

Agriculture, Biological Sciences & Forestry

Agritech with registered number ISSN 0216-0455 (print) and ISSN 2527-3825 (online) is a scientific journal that publishes the results of research in the field of food and agricultural product technology, agricultural and bio-system engineering, and agroindustrial technology. This journal is published by Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta in colaboration with Indonesian Association of Food Technologies (PATPI).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 1,392 Documents

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Pediococcus acidilactici F-11 Penghasil Bakteriosin sebagai Agensia Biokontrol Escherichia coli dan Staphylococcus aureus pada Sayuran Segar Simpan Dingin Endang Sri Rahayu; Eni Harmayani; Tyas Utami; Kejora Handarini
agriTECH Vol 24, No 3 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Our survey indicated that fresh vegetables contained. high population of microorganisms including pathogenic Staphylococcus aureus. Washing procedures, including the addition of sanitizes to the wash water have not been effectively in reduction of the number of microorganisms. Currently, there is interest in possible use bacteriocin producer of lactic acid bacteria as biocontrol agents to ensure safety of minimally processed, refrigerated (MPR) foods which are not acidified, including fruits and vegetables. Our previous result, indicated that Pediococcus acidilactici F-11 (PAF-11) produced bacteriocin with wide spectrum activity. Objectives of this research was to study the potency of PAF-I 1 as biocontrol agent to inhibit the growth of pathogenic bacteria occurred in ready to eat fresh vegetables, i.e., paprika, lettuce, carrot. The results showed that PAF-11 was able to inhibit the growth of naturally present coliform and Staphylococcus significantly, as well as that of tested bacteria of E. coil and S. aureus which were inoculated into paprika and carrot. PAF-11 was able to grow at these two vegetables, and their population were increased about I log cycle. Bacteriocin activity produced by PAF-11 was positively detected from these two vegetables inoculated with these bacteria. However, there was no inhibition activity of PAF-11 against naturally present coliform and Staphylococcus, as well as inoculated E. coil and S. aureus on lettuce. Bacteriocin activity produce by PAF-11 was also not detected in this vegetables. Conclusion of this study, Pediococcus acidilactici F-11 could be used as biocontrol agents in paprica and carrot.

Ekstrak Air Jahe (Zingiber officinale Roscoe) Menghambat Akumulasi Kolesterol pada Makrofag Aisyah Tri Septiana
agriTECH Vol 24, No 3 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Water extract of ginger rhizomes has been reported to protect low density lipoprotein (LDL) oxidation. We investigated the effect of these extract enrichment on the prevention of cholesterol accumuation in macrophage. Water extract of ginger rhizomes was suplemented on LDL isolate (2150 idg/m1 LDL), oxidized with CuSO4 and incubated on macrophage culture. These extract also was suplemented on macrophage culture before incubated with LDL and FeCl2. The corresponding esterified cholesterol accumulation was analized from macrophage culture. Result showed that water extract of ginger rhizomes suplementation on LDL isolated before CuSO4 oxidation and on medium macrophage culture before incubation with LDL and FeCl2 reduced 69,07 % and 43,21 % of esterified cholesterol accumulation from macrophage, respectively. This research has shown that water extract of ginger rhizomes is capable of prevention cholesterol accumulation in macrophage.

Simulation of Root-Matted Cutting by Use of Distinct Elemet Method Bambang Purwantana
agriTECH Vol 24, No 3 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

A numerical technique, the distinct element method (DEM.l, was used to simulate the mechanical behavior of root-matted soil. The conventional DEM was modified with introducing a tensile parameter on the mechanical relationship between the DEM elements. The validity of the modified model was investigated by conducting an experiment in which root-matted soil is cut by a blade. The simulation results indicate the appropriateness of the modified DEM The effects of tension from the experimental results could be simulated by the DEM. This study has provided new and valuable information on the behavior of soil and root at cutting process.

Aspergillus Proteolitik Indigenous dari Koji dan Kemampuannya Mendegradasi Aflatoksin B1 Sardjono Sardjono; Endang Sri Rahayu; Sri Raharjo; Kapti Rahayu Kuswanto
agriTECH Vol 24, No 3 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Legume and cereals are always exposed to the danger of fungal contamination. Among such fungi, some species of the genus Aspergillus are potential of aflatoxins producer. Aflatoxin BI (AFB1) which is the most carcinogenic mycotoxins , known very stable under cooking condition and other processing factors. The removal of AFB1 by degradation or detoxification is critical to reduce risk to human health. Microbiological degradation is a promising method for AFB1 degradation compared to others. The aim of this research was to isolate the proteolytic Aspergillus strain from "koji" and to determine its ability to degrade AFB). Out of 18 strains of Aspergillus, 16 strains were found proteolytic and only 5 strains had no afiatoxigenic properties, but all of them were able to degrade AFB1. There were no spesific pattern of the rate of AFBI degradation. Strain of KKB4 was identified as Aspergillus oryzae, that possess the highest ability to degrade AFB1. Two kind of substances were formed after degradation which were more polar than AFB1. The rate of AFB) uptake by Aspergillus oryzae KKB4 was similar with that of mycelia! growth. Aflatoxin BI inhibits mycelium growth, vesicle and conidial head formation.

Sebaran Kinerja Daerah Irigasi Pasca Penyerahan Pengelolaan Irigasi Murtiningrum Murtiningrum; Wisnu Wardana; Ni Nyoman Sulastri
agriTECH Vol 24, No 3 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

One among the policies in Irrigation Management Policy Reform (IMPR) is the Irrigation Management Transfer (IMT). Through the IMT, farmers in Water Users' Association (WUA) manage their own irrigation systems. The IMT let farmers to diversify their farming system to achieve farmers' welfare. The diversification results in in the variation of planting pattern in an irrigation system. The variation is bigger if market plays a role in crop choice. Therefore, the assumption that an irrigation system has a uniform performance throughout the system is no longer valid. This paper aims to examine the distribution of an irrigation system managed by farmers. The system chosen as sample was Mejing Irrigation System in Banjul, Yogyakarta. The performance was assessed by using adequacy and water delivery performance. The spatial distribution of Mejing Irrigation System is mainly affected by irrigation operation pattern as well as condition and serviceability of irrigation network Spatially, the tertiary blocks located in the head receive more water than other tertiary. blocks in the tail.

Kajian Perilaku Pengeringan Sari Buah dengan Pengering Semprot Searah Ansar Ansar; Suhargo Suhargo; Budi Raharjo
agriTECH Vol 24, No 3 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

This study aimed to examine the behavior of concurrent spray drying of fruit juice on various inlet temperature of the drying air and atomization air pressure, The temperature of inlet drying air ranged approximately from 115 °C and 135 °C. The atomization air pressures were varied from 1,8593; 1,8746; and 1,9245 Pa. The data of this study consist of predicted and observed data. Observed data obtained from laboratory experiment which included the distribution of drying air temperature as along the axial distances and the average moisture content of final product. Predicted data obtained to simulate models based on three mathematical models consists of mass, momentum, and energy balance equation. The mathematical models were employed to predict the temperature of drying air distribution and the average of moisture content offinal product. The results of the study showed thatj the mathematical models represent of the behavior of fruit juice drying using concurrent spray dryer. On the same material bait; number, size, and droplet distribution were changed on the change of atomization air pressures and air temperature drying. The higher was the atomization air pressure and the air temperature which were used, the greater was the droplet which were product.

Perancangan Tata Letak Vertikal Pembuatan Emping Melinjo Guntarti Tatik Mulyati; Wagiman Wagiman; A.M. Madyana
agriTECH Vol 24, No 2 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Melinjo crackers small-scale industries are among the industries which still adopt manual and traditional production processes. The research is aimed at improving layouts of a melinjo cracker small scale industry using workers' vertically anthropometrical data. The research was initiated by measuring the workers, productivity and observing the horizontal layouts before the modification. Anthropometrical data were collected by measuring the dimension of woman's body. Such data would be used to design the new layouts by considering then heights of equipment and of the workers 'sitting position. The results show that a better layout arrangement could be achieved. The workers worked by sitting on a chair with the heights of the table and the chair tailored to the worker's anthropometrical data. The position of the equipment was within the workers range of movements, the pealing and the crushing tools were within the normal working area, while the frying and the raw material were on the maximum working area. The workers felt more comfortable when doing their works.

Pengaruh Penambahan NaCl sebagai Stress Agent dalam Kultivasi Sel Mikroalga Dunaliella tertiolecta ATCC 30929 terhadap Akulumasi Lipid Intrasel Karseno Karseno; M. Takagi; T. Yoshida
agriTECH Vol 24, No 2 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Lipid composition of microalgae cell was affected by saline medium. Increasing of initial NaCI in modified NORO medium from 0.5 M to 1.0 M resulted in an increase of intracellular lipid content of Dunaliella tertiolecta ATCC 30929 cell from 60% to 67% (db). Loading of 0.5 M NaCI at a middle of logarithmic phase or 1.0 M at the end of logarithmic phase during the growth on the same medium increased intracellular lipid content to 70% (db). Addition of NaCI up to 2.0 M during the culture gave a high intracellular lipid content up to 77% (db) , although cell concentration was decreased to a half The temperature, light intensity, and aeration was adjusted at 30 Â°C, 150 mo1.11.m-2 (10.000 lux) and 250 ml/min CO2 enriched air (CO2 3%).

Produksi Asam Asetat oleh Sel Acetobacter pasteurianus INT-7 Amobil pada Variasi Konsentrasi Etanol Sri Luwihana; Endang Sri Rahayu; Slamet Sudarmadji; Kapti Rahayu
agriTECH Vol 24, No 2 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Acetic acid fermentation by Acetobacter pasteurianus 1NT-7 immobilized cells with alginate gel on various ethanol concentration was investigated. Immobilized acetic acid bacteria cells were prepared with alginate gel 3% concentration with ratio cell suspension and alginat gel 1:3 (v/v) and conditioning time 24 hours. Fermentation using 10% immobilized cells (beads), medium of fermentation is YEPE (yeast extract pepton ethanol) with three ethanol concentration 5%, 7,5% and 10% respectively. Fermentation is going on shaker incubator with condition 100 rpm, 30" C for 10 days. The acetic acid production, pH and cells number were monitored for eveï€´y day. The immobilized cell fermentation was compared with free cells fermentation. The yield of free cells fermentation on 5% ethanol concentration is higher (84,6%) than immobilized cells yield (64,25 %), but on 7,5% and 10% ethanol concentration the immobilised cells yield is higher (62,77% and 45,44% respectively) than free cells yield ( 61,23% and 2,54% respectively).So the acetic acid fermentation using immobilized cells is effective on 7,5% ethanol concentration, while fermentation using free cells is effective on 5% ethanol concentration

Skrining Bakteri Asam Laktat Penghasil Bakteriosin dari Daging dan Produk Olahannya Endang Sri Rahayu; A.K. Wardani; S. Margino
agriTECH Vol 24, No 2 (2004)
Publisher : Faculty of Agricultural Technology, Universitas Gadjah Mada, Yogyakarta, Indonesia

Lactic acid bacteria (LAB) which naturally occur in meat and meat products have been isolated and screened for their ability to produce bacteriocin. The objective of this research was to obtain the potential bacteriocin producer of lactic acid bacteria which could be used as food bio-preservative. Source of lactic acid bacteria used in this study were beef chicken flesh, 'vacuum packaged' sausage and sliced meat obtained from traditional market or department store. Ten grams of each samples was put onto five different enrichment media, i.e., TGE (tryptoneglucose-yeast extract) pH 5 plus 3% NaCl; MRS (deMan Rogose Sharpe) pH 5,5; TGE broth pH 5,5; TGE buffer broth pH 5,5; and TGE broth plus Tween 80 & 1% Naazida pH 6,0, incubated for 24-71 hours to stimulate the growth of lactic acid bacteria. Different enrichment media were used to stimulate the growth of strains belong to each genus, since the nutritional and environmental requirement for optimum growth were suggested to be genera-dependent. Screening of LAB bacteriocin producer was carried out by dilution -pour plate methods (culture from each enrichment medium) followed by overlay using the indicator strains. Indicator strains used in this study were Lactobacillus plantarum NCDO 955, Pediococcus acidilactici LB-42, Leuconostoc mesenteroides LY, and Enterococcus faecalis MI. Colonies showing growth inhibition to indicator (indicated by clear zone) were isolated and purified. Isolates were then characterized based on Gram, catalase, shape and arrangement of cell, type of fermentation, effect of temperature to the growth and acid production from several carbon sources. From the primary screening (dilution - pour plate â€“overlay), 30 strains belong to Lactobacillus, Leuconostoc, Streptococcus and Enterococcus which suspected to produce antimicrobial substance were obtained. However, based on the confirmation lest (dUsion method), only three (3) strains were identified to produce bacteriocin. i. e. Leuconostoc mesenteroides SM 22, SM 32, and SM 46. In this study, Leuconostoc mesenteroides SM 22 was selected for food application. Bacteriocin of Leuconostoc mesenteroides SM-22 was able to inhibit the growth of psychrophilic bacteria naturally occur in meat and shrimp kept at refrigerator. Microbial population of raw meal with the initial number of about 3x104 CFU/g decreased one log cycle after treated with bacteriocin, and this number maintained less than 105 CFU/g after storage raw meat at refrigerator for five days. On the other side, microbial population of raw meat with no bacteriocin treatment increased to 106 CFU/g after 4 days kept at refrigerator. In the case of shrimp, washing raw shrimp with cold water could reduce the population of bacteria about one log cycle, followed treatment with bacteriocin, this populationincreased very slowly and still less than 105 CFU/g after 5 days storage at refrigerator. While without any treatment, microbial population of raw shrimp which initially about 3x105 CFU/g rapidly increased to 106 CFU/g after 3 days. This data showed that Leuconostoc mesenteroides SM-22 was a potential bacteriosin producer and can be applied as bio-preservative for cold storage fbod.

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Contact Name
Fiametta Ayu Purwandari

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fiametta@ugm.ac.id

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+6285712601130

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agritech@ugm.ac.id

Editorial Address
Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA

Abstract

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Abstract

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Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Fiametta Ayu Purwandari

Contact Email fiametta@ugm.ac.id

Phone +6285712601130

Journal Mail Official agritech@ugm.ac.id

Editorial Address Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia

Location Kab. sleman, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Fiametta Ayu Purwandari

Contact Email
fiametta@ugm.ac.id

Phone
+6285712601130

Journal Mail Official
agritech@ugm.ac.id

Editorial Address
Faculty of Agricultural Technology, Universitas Gadjah Mada, Jl. Flora No. 1, Bulaksumur, Yogyakarta 55281, Indonesia

Location
Kab. sleman,

Daerah istimewa yogyakarta

INDONESIA