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Biocelebes
Published by Universitas Tadulako
ISSN : -     EISSN : 25805991     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 7 Documents
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Search results for , issue "Vol. 16 No. 2 (2022)" : 7 Documents clear
FITOKIMIA, DAN AKTIFITAS ANTIBAKTERI DARI Etlingera sublimata Poulsen (ZINGIBERACEAE), TUMBUHAN ENDEMIK SULAWESI: Phytochemical and Antibacterial Activity of Etlingera sublimata Poulsen (Zingiberaceae), Endemic Plant to Sulawesi Ramadanil Pitopang; Endang Lestari; PUTI ANDALUSIA SARIGANDO Banilai; Wahyu Harso
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.15957

Abstract

The research entitled "Phytochemical Screening and antibacterial activity of Etlingera sublimata Poulsen (Zingiberaceae), an Endemic Sulawesi of Plants was conducted from January 2020 to June 2020, at the Plant Biosystematics Laboratory and Biotechnology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. Samples of E. sublimata were collected from the mountane forests of the Lore Lindu National Park (LLNP), arround Sedoa village, Lore Utara District, Poso Regency The objectives of the research were to analyze the secondary metabolite compounds in E. sublimata plants and to determine the antibacterial activity of E. sublimata leaves extract in againts Salmonella thypii bacteria. Plant samples were extracted by maceration methods with 96% ethanol as a solvent. Actibacterial activity was tested by using agar well diffusion methods. The experiment was designed by Completely Randomized Design (CRD) with seven different concentration of E. sublimata extract (15, 25, 50, 75 and 85%). Extract and standard drugs were prepared in double-distilated water using Nutrient Agar tubes. 2% Chloramphenicol as standard drugs was used as a positive control and sterile aquadest was used as a negative control. The inhibition zone of bacteria and yeast growth around the disk was measured after 18 to 24 h incubation at 37°C. The results showed that the stem contains alkaloids, flavonoids, terpenoids, tannins and saponins. The rhizome containts alkaloid, flavonoids, terpenoids, tannins and saponins, the flowers containts alkaloid, terpenoids and tannins, while the leaves containts tannin and saponin compounds. Leaves extract of E.sublimata has inhibition activity on the cell growth a pathogenic Salmonella thypi bacteria that the effective extract concentration was 50% with an average inhibition zone ​​2.67 mm.
TINGKAT SERANGAN DAN PADAT POPULASI WALANG SANGIT (LEPTOCORISA ORATORIUS) DI DISTRIK TANAH MIRING KABUPATEN MERAUKE: TINGKAT SERANGAN DAN PADAT POPULASI WALANG SANGIT (LEPTOCORISA ORATORIUS) DI DISTRIK TANAH MIRING KABUPATEN MERAUKE jefri sembiring
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.15987

Abstract

This study aims to determine the attack rate and population density of the paddy bug (Leptocorisa sp). The method used in this research is purposive sampling. The results showed that the intensity of damage caused by paddy bugs in Tanah Miring District was 13.78%. The highest average population density was in Waninggap Miraf 46.6 individuals, Yasamulya and Isanombias villages 44.33, Yabamaru 38.6, Bersehati 38.3 and the lowest Ammunay 30. While the average population in each observation was the second observation or 55 DAP as many as 46.4 tails followed by observations 1 or 45 DAP as many as 37.4 and the last observation or 65 DAP at 29.8 individuals. The type of variety that was most favored by the paddy bug was impari 19.75% and the one that pandanus did not like. Keywords: Attack rate, population density, paddy bug, paddy
COMPOSITION AND AMOUNT OF MARINE DEBRIS DISTRIBUTED TO MANGROVE ECOSYSTEMS IN KUPANG CITY, EAST NUSA TENGGARA, INDONESIA lumban nauli lumban toruan; Selviana Mboro; Lady Cindy Soewarlan
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.16061

Abstract

The unique mangrove root system is a gathering place for small and large waste. The density of the population results in varied activities so that the waste produced in various types, quantities and sizes can be distributed to the mangrove area either through rivers or carried by the wind. The purpose of this study was to determine the type and amount of waste in the mangrove ecosystem in Kupang City. The method used is observation. Sampling was carried out by installing a transect perpendicular to the sea along 70 m at the place with the highest abundance of waste and the thickness of the mangrove reached 50-100 meters. There are 6 transects installed at the study site and each transect is divided into three zones consisting of three quadrants with a size of 30 x 10 m2. The distance between quadrants is 20 m with the first quadrant being at the first point where mangroves are found. The results of the study found that the composition of the types of waste scattered in the mangrove ecosystem of Kupang City were 88,91% plastik with a total of 15,628 pieces, cloth (4,21%) with a total of 640 pieces, other waste (3,97%) with a total of 604 pieces, cork/foam (1,18%) with a total of 148 pieces, rubber (0,57%) with a total of 86 pieces, metal (0,29%) with a total of 44 pieces, glass and ceramics (0,15%) with a total of 11 pieces, then paper and cardboard (0,05%) with a total of 7 pieces. Types of plastic waste, fabrics, others, rubber and metal have a greater amount in the first zone, cork / foam is the most in the second zone then paper and cardboard are most found in the first and third zones, while glass and ceramics are the most in zone three.
STUDI TUMBUHAN BERKHASIAT OBAT PADA SUKU DONDO DI KECAMATAN OGODEIDE, KABUPATEN TOLITOLI, PROVINSI SULAWESI : Study of Drug Effective Plants on The Dondo Tribe in Ogodeide District, Tolitoli Regency, Central Sulawesi Province Syariful Anam; Agustinus Widodo; Muhamad Rinaldhi Tandah
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.16063

Abstract

The Dondo Tribe is an indigenous tribe in Ogodeide District. The Dondo Tribe still uses traditional medicine to treat various diseases. The high trust of the Dondo people towards the use of traditional medicine makes people prefer to use traditional medicine. This study aimed to determine the types, parts, and methods of use of plants that have been used as traditional medicine by the bolrians of the Dondo tribe, Ogodeide District, Tolitoli Regency, Central Sulawesi Province. This type of research was descriptive qualitative and the sampling technique was purposive sampling, with structured interview techniques on 11 informants by filling out questionnaires with supporting documentation. The results of the study found that as many as 40 species of plants used as traditional medicine by the bolrians of the Dondo tribe in Ogodeide District which are still preserved today. The percentages of the most widely used plant parts were leaves 55%, stems 11%, rhizomes 11%, roots 5%, fruit 5%, herbs 5%, bark 2%, twigs 2%, tubers 2% and flowers 2%. The percentage of how to use plants as traditional medicine is 65% taken, 26% affixed, 3% seen, 3% washing and 3% smeared.
INOVASI UBI KAYU TRANSGENIK MENGGUNAKAN TEKNOLOGI CRISPR-CAS9 SEBAGAI UPAYA PEMENUHAN KEBUTUHAN PANGAN MASA DEPAN May Ningsih Wigati; Nurul Fajar Amini; Esna Dilli Novianto
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.16112

Abstract

Jumlah penduduk yang kian meningkat menyebabkan kebutuhan pangan terus meningkat. Hal ini menyebabkan angka ketergantungan akan beras juga meningkat. Padahal hingga saat ini, untuk memenuhi kebetuhan bahan pangan beras, pemerintah masih harus melakukan impor. Untuk itu sebagai upaya menurunkan ketergantungan konsumsi beras masyarakat Indonesia, pemerintah menggalakkan program diverfisikasi pangan. Salah-satu komoditas yang difokuskan dalam program ini yaitu ketela pohon atau ubi kayu. Namun, dalam pemenuhan kebutuhan gizi, ketela pohon masih memiliki kandungan protein yang rendah. Untuk itu dilakukan genome editing dengan teknik CRISPR-Cas9. CRISPR-Cas9 ini adalah teknologi yang bekerja untuk pengeditan genom dengan kemampuan menemukan tempat tertentu di DNA, untuk memotong sebagian kecil DNA di tempat itu, dan mengedit DNA tersebut. Penerapan teknik CRISPR-Cas9 terhadap ketela pohon menghasilkan tanaman yang mengandung protein yang lebih tinggi.
ANALISIS KADAR TIMBAL (Pb) PADA BERBAGAI TUMBUHAN DI KAWASAN STASIUN PENGISIAN BAHAN BAKAR UMUM (SPBU) KOTA PALU Muhammad Fajri Ramadhan Muslim Saleh; Miswan Miswan; Riska Septiana
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.16132

Abstract

Exhaust gas produced by motorized vehicles releases lead (Pb) into the environment and can be absorbed into plant tissues. This study aimed to examine the content of lead (Pb) accumulated in leaves. Sampling used purposive sampling as a sampling method at four gas stations in Palu City. Pb levels were determined by atomic absorption spectrophotometry (AAS) at the Laboratory of Analytical and Environmental Chemistry, Department of Chemistry, Faculty of Mathematics and Natural Sciences, Tadulako University. The results showed that the highest to lowest lead absorption in the leaves was Syzygium myrtifolium Walp; 0.75 mg/l from Tawaeli gas station, Bouganvillea spectabilis Willd; 0.67 mg/l from Maluku gas station, Polyalthia longifolia var. pendula; 0.67 mg/l at Yos Sudarso gas stations, and Dracaena reflexa Lam; 0.58 mg/l from Diponegoro gas station. The concentration of lead (Pb) found in each plant from gas stations is still below the maximum threshold set by SNI 01-7387:2009, which is 0.5-3 ppm. The ambient air content around the gas station is still below the air quality standard for measuring the lead (Pb) 2 g/m3 parameter.
PERTUMBUHAN TANAMAN BAYAM AKAR MERAH (AMARANTHUS RETROFLEXUS) YANG DIINOKULASI DENGAN BERBAGAI JENIS JAMUR MIKORIZA ARBUSKULA YANG DITUMBUHKAN PADA TANAH TERCEMAR MERKURI Wahyu Harso; Devan Prima
Biocelebes Vol. 16 No. 2 (2022)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v16i2.16249

Abstract

Bayam akar merah (Amaranthus retroflexus) mampu tumbuh pada tanah tercemar logam berat tanpa menunjukkan symptom. Jamur mikoriza dapat meningkatkan pertumbuhan tanaman pada tanah tercemar logam berat. Tujuan penelitian ini yaitu mengetahui pengaruh pemberian jamur mikoriza arbuskular dari jenis yang berbeda terhadap pertumbuhan tanaman bayam pada tanah tercemar merkuri. Pada penelitian ini tanaman bayam diinokulasi dengan inokulum tunggal dari jenis Glomus sp., Acaulospora tuberculata, dan inokulum campuran dari jenis Acaulospora, Glomus, Gigaspora, dan Scutelospora dan tanpa pemberian inokulum sebagai kontrol. Tanaman bayam ditumbuhkan pada tanah yang tidak dicemari dan dicemari dengan logam merkuri sebanyak 8 mg HgCl/kg tanah. Hasil penelitian menunjukkan bahwa pertumbuhan tanaman bayam tidak dipengaruhi oleh tanah yang tercemar merkuri. Pemberian inokulum jamur mikoriza arbuskula cenderung menurunkan pertumbuhan tanaman. Tidak ada perbedaan yang nyata pada jenis jamur yang diberikan dalam menurunkan pertumbuhan tanaman bayam.

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