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All Journal HAYATI Journal of Biosciences Jurnal Ilmiah Farmasi Media Eksakta Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Biocelebes KOVALEN: Jurnal Riset Kimia Journal of Islamic Pharmacy Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) Indonesian Journal of Chemistry GHIDZA: Jurnal Gizi dan Kesehatan Indonesian Fisheries Research Journal Jurnal Ilmu Kefarmasian Indonesia Jurnal Mandala Pengabdian Masyarakat Jurnal DiMas (Jurnal Pengabdian Kepada Masyarakat) Pharmaceutical Reports Jurnal Pengabdian Farmasi dan Sains Jurnal Dedikatif Kesehatan Masyarakat Jurnal Farmasi Sains dan Terapan (Journal of Pharmacy Science and Practice) Poltekita: Jurnal Pengabdian Masyarakat
Agustinus Widodo
Jurusan Farmasi, Fakultas MIPA, Universitas Tadulako, Palu, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Earth & Planetary Sciences Education Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Other

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Prediksi Farmakokinetik, Toksisitas, dan Aktivitas Enzim Protease HIV-1 Inhibitor dari Daun J. gendarussa Widodo, Agustinus
Jurnal Farmasi Sains dan Terapan Vol 6, No 1 (2019)
Publisher : Jurnal Farmasi Sains dan Terapan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (353.098 KB) | DOI: 10.33508/jfst.v6i1.2005

Abstract

Justicia gendarussa Burm.f. (Acanthaceae) has been known to have anti-HIV activity. This study was conducted to determine the interaction of flavonoids (gendarusin A, B, C, D, and E) on the J. gendarussa leaves against HIV-1 protease receptor. It is expected that this research will provide scientific information on the development of J. gendarussa leaves as an anti-HIV drug. This study used in silico testing methods with FAF-Drugs4 to predict the ADMET (absorption, distribution, metabolism, and excretion–toxicity), and Molegro Virtual Docker (MVD) to predict the activity of five gendarusin compounds to the receptor of HIV-1 protease (PDB ID: 4HLA). The activity prediction was reflected by hydrogen bond and steric bond visible in MVD program with amino acid residue of the receptor of HIV-1 protease. Gendarusin compounds had good oral bioavailability and were not toxic, and from molecular docking test, it was found that gendarusin of J. gendarussa leaves could inhibit the activity of HIV protease enzyme. Gendarusin of J. gendarussa has potential as an anti-HIV.
PHYTOCHEMICAL SCREENING, TOTAL FLAVONOID, ANTIOXIDANT ACTIVITY, AND TOXICITY OF ETHANOL EXTRACT Cleome gynandra L. HERB Widodo, Agustinus; Pratiwi, Ritha
Journal of Islamic Pharmacy Vol 3, No 2 (2018): J. Islamic Pharm.
Publisher : Universitas Islam Negeri Maulana Malik Ibrahim Malang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18860/jip.v3i2.6101

Abstract

Cleome gynandra L. is one of the plants that the people of Palu, Central Sulawesi use as food ingredients. This plant is empirically used in traditional medicine. This study aims to determine phytochemical constituents, total flavonoid, antioxidant activity, and toxicity of 96% ethanol extract of C. gynandra herb. C. gynandra herb extract was obtained by maceration. Phytochemical screening of the ethanol extract was carried out qualitatively according to the standard methods. Determination of total flavonoid using AlCl3 then determined by Spectrophotometric UV-Vis. Antioxidant activity using the DPPH method and determined IC50 value. Toxicity test was assessed using shrimp lethality as an indicator of toxicity. Phytochemical screening showed 96% ethanol extract containing alkaloid compounds, flavonoids, saponins, steroids, and tannins. Total flavonoid of the 96% ethanol extract was 4,778 ± 0,522 mg QE/g extract. Antioxidant activity (IC50) of the ethanol 96% extract was 189,455 µg/ml. Lethal concentration 50% of the 96% ethanol extract was 472,648 mg/L (toxic). The results of this study indicate that 96% ethanol extract of C. gynandra herb has antioxidant activity and has the potential to be further tested as an anticancer activity.
Pengaruh Infus Herba Meniran (Phyllanthus niruri L) Terhadap Daya Larut Kalsium Batu Kandung Kemih Widodo, Agustinus
Media Eksakta Vol 7, No 1 (2011)
Publisher : Media Eksakta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (12.331 KB)

Abstract

The aim of this research is to determine the most effective concentration of “meniran” herb infuse in drawn–out the calcium of bladder stone.  The concentration of infuse was varied in 2 %, 6 %, 10 %, 14 % and 18 % w/v, then they were added with 100 mg of the bladder stone.  The calcium analysis was done by Atomic Absorption Spectrophotometer and this study was formatted in the Completely Randomized Design.  The F test indicate that “meniran” herb infuse increase the calcium solubility of bladder stone significantly and the Duncan’s multiple range test also show that the most effective concentration is 10 % w/v.
Isolation of endophytic fungi from benalu batu (Begonia medicinalis) and their toxicity on Artemia Salina Syariful Anam; Armini Syamsidi; Musyahidah Musyahidah; Nurul Ambianti; Agustinus Widodo; Muhammad Sulaiman Zubair
Jurnal Ilmiah Farmasi 2022: Special Issue
Publisher : Universitas Islam Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/jif.specialissue2022.art3

Abstract

Abstract   Background: Endophytic fungi are microorganisms found in plant tissues that are mutually beneficial to the host plant. They are known as sources of bioactive metabolites which possess various biological activities such as antimicrobial, anticancer, antiviral, antifungal, and antioxidant.  Benalu batu (Begonia medicinalis) has been used empirically as a medicinal plant and has shown anticancer activity in breast cancer cells (T47D cells) and cervical cancer cells (HeLa cells).Objective: This study aimed to isolate the endophytic fungi from benalu batu and determine the toxicity activity and Lethal Concentration 50% (LC50) value of isolated endophytic fungi using shrimp larvae (Artemia salina). Method: Endophytic fungi were isolated from fresh parts; leaves, stems, roots of Benalu Batu. The selected parts were sterilized with ethanol 70% and sodium hypochlorite 1% subsequently placed into Potato Dextrose Agar Chloramphenicol (PDAC) then incubated at 28°C for 7 days. Isolated endophytic fungi were extracted by sonication method using ethyl acetate. The toxicity activity of isolated endophytic fungi was determined using the Brine Shrimp Lethality Test (BSLT).Results: Isolation of the endophytic fungi from benalu batu obtained 10 isolates, which the leaves part obtained 6 isolates, the stem obtained 3 isolates and the root obtained 1 isolate. The results of screening for toxicity test of 10 isolates at concentrations of 500 µg/mL shown that isolate D3, D5, and D6 gave 100% mortality. The LC50 values of isolate D3, D5, and D6 were obtained respectively in 448.03, 8.784, and 9.13 µg/mL.Conclusion: These results indicated that isolate D5 exhibits the greatest toxicity and needs to be investigated the activity for testing on cancer cells.Keywords: endophytic fungi, Begonia medicinalis, toxicity, Artemia salina Intisari  Latar belakang: Jamur endofit merupakan mikroorganisme yang terdapat dalam jaringan tumbuhan yang bersifat mutualisme terhadap tumbuhan inang. Jamur endofit telah diketahui sebagai sumber metabolit yang memiliki beberapa aktivitas biologi seperti antimikroba, antikanker, antivirus, antijamur, dan antioksidan. Tumbuhan benalu batu (B. medicinalis) telah digunakan secara empiris dan terbukti memiliki aktivitas antikanker pada sel kanker payudara (Sel T47D) dan sel kanker leher rahim (Sel HeLa). Tujuan: Studi ini bertujuan untuk melakukan isolasi jamur endofit dari benalu batu dan mengetahui toksisitas isolat jamur endofit serta menentukan nilai Lethal Concentration 50% (LC50) terhadap larva udang (Artemia salina)Metode: Jamur endofit diisolasi dari bagian segar; daun, batang, akar benalu batu. Bagian-bagian tersebut disterilisasi dengan etanol 70% dan natrium hipoklorit 1% selanjutnya diletakkan pada media Potato Dextrose Agar Chloramphenicol (PDAC) kemudian diinkubasi pada suhu 28°C selama 7 hari. Isolat jamur endofit diekstraksi dengan metode sonikasi menggunakan etil asetat. Aktivitas toksisitas isolat jamur endofit ditentukan menggunakan metode Brine Shrimp Lethality Test (BSLT).Hasil: Isolasi jamur endofit dari benalu batu diperoleh 10 isolat, bagian daun diperoleh 6 isolat, bagian batang diperoleh 3 isolat dan bagian akar diperoleh 1 isolat. Hasil penapisan uji toksisitas 10 isolat pada konsentrasi 500 µg/mL menunjukkan bahwa isolat D3, D5, dan D6 memberikan persentase mortalitas sebesar 100%. Nilai LC50 isolat D3, D5, dan D6 diperoleh masing-masing sebesar 448,03, 8,784, dan 9,13 µg/mL.Kesimpulan: Hasil ini menunjukkan isolat D5 memiliki toksisitas paling besar dan perlu dilanjutkan untuk pengujian pada sel kanker.Kata kunci : jamur endofit, Begonia medicinalis, toksisitas, Artemia salina
PENGARUH PENAMBAHAN AKTIVATOR DALAM PEMBUATAN KARBON AKTIF AMPAS TAHU SEBAGAI ADSORBEN MINYAK JELANTAH Ma'rifah Ma'rifah; Jamaluddin Jamaluddin; Yonelian Yuyun; Agustinus Widodo
KOVALEN: Jurnal Riset Kimia Vol. 4 No. 1 (2018): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (612.489 KB) | DOI: 10.22487/kovalen.2018.v4.i1.9363

Abstract

Minyak goreng yang telah digunakan berulang kali akan mengalami kerusakan seperti peningkatan bilangan peroksida dan asam lemak bebas yang dapat menimbulkan bahaya bagi kesehatan. Penelitian ini memvariasikan aktivator pada proses pembuatan karbon aktif ampas tahu yaitu NaCl 10%, Na2CO3 5% dan H3PO4 1M untuk mengetahui pengaruhnya terhadap kadar asam lemak bebas dan bilangan peroksida dalam minyak jelantah. Minyak yang digunakan adalah minyak goreng kemasan yang digunakan untuk menggoreng ikan kering, ikan basah, tahu-tempe dan daging ayam. Tahapan pemurnian minyak yaitu proses despising, netralisasi dan bleaching. Pengujian mengacu pada SNI (Standar Nasional Indonesia) 7709:2012 sebagai syarat mutu minyak goreng. Hasil penelitian menunjukkan kadar asam lemak bebas tertinggi dan terendah yang diperoleh yaitu 0,1925% dan 0,1473%, sedangkan bilangan peroksida tertinggi dan terendah yaitu 9,3203 meq O2/kg dan 4,3526 meq O2/kg. Aktivator yang paling berpengaruh pada penurunan kadar asam lemak bebas dan bilangan peroksida adalah Na2CO3 dimana diperoleh kadar rata – rata asam lemak bebas 0,1473% dengan persentase penurunan sebesar 57,68% dan kadar  rata – rata penurunan bilangan peroksida yaitu 4,3526 meq O2/kg dengan persentase penurunan sebesar 59,04%. Jadi, aktivator Na2CO3 merupakan aktivator yang paling baik digunakan untuk aktivasi karbon ampas tahu sebagai adsorben minyak jelantah.Kata Kunci:    Aktivator, Karbon Aktif, Ampas Tahu, Adsorben, Minyak Jelantah. 
Analisis Kualitatif dan Kuantitatif Metabolit Sekunder Ekstrak Etanol Buah Okra (Abelmoschus esculentus L. Moench) dengan Metode Spektrofotometri UV-Vis: Qualitative and Quantitative Analysis of Secondary Metabolites in Ethanol Extract of Okra (Abelmoschus esculentus L. Moench) Fruit with UV-Vis Spectrophotometry Method Joni Tandi; Bella Melinda; Anita Purwantari; Agustinus Widodo
KOVALEN: Jurnal Riset Kimia Vol. 6 No. 1 (2020): Edisi April
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2020.v6.i1.15044

Abstract

This study aims to determine the content of secondary metabolites and the levels of total secondary metabolites in ethanol extract of Okra (Abelmoschus esculentus L. Moench) fruit with UV-Vis Spectrophotometry method. Okra (Abelmoschus esculentus L. Moench) fruit extract was prepared by maceration method with 96% ethanol. The extract was concentrated using a rotary evaporator that followed by a qualitative test for alkaloids, flavonoids, saponins, and tannins using suitable reagents with the test parameters, while for the qualitative assay using a UV-Vis Spectrophotometry. Qualitative analysis of alkaloids using test parameters of total alkaloids equivalent to quinine, flavonoid using parameter of total flavonoids equivalent to quercetin, saponin using parameters of standard saponins total from the Quillaja Bark, and tannin using test parameters of total tannins equivalent to tannic acid. Qualitative test results indicated that the ethanol extract of Okra fruit tested positive for alkaloids content wich characterized by the orange deposition, flavonoids characterized by the formation of an orange-yellow color, a stable foam for saponins and tannins marked in black. The quantitative analysis resulted alkaloids of 2228.06 mg/gram, flavonoids of 2.79 mg/gram, saponins of 10.03 mg/gram, and tannins of 1973.27 mg/gram. Keywords: Okra fruit, secondary metabolites, UV- Vis spectrophotometry, qualitative, quantitative.
Analisis Metabolit Sekunder dan Aktivitas Ekstrak Etanol Kulit Buah Salak (Salacca zalacca (Gaertn.) Voss) Terhadap Kadar Glukosa dan Ureum Kreatinin Tikus Putih Jantan (Rattus norvegicus): Analysis of Secondary Metabolites and Activity of Ethanol Extract of Snake Fruit Peel (Salacca zalacca (Gaertn.) Voss) on Glucose and Ureum Creatinine Levels of Male White Rats (Rattus norvegicus) Tien Wahyu Handayani; Agustinus Widodo; Risna Yanti; Erdy Prasetyo; Zulfaidah; Joni Tandi
KOVALEN: Jurnal Riset Kimia Vol. 7 No. 3 (2021): Edisi Desember
Publisher : Chemistry Department, Mathematics and Natural Science Faculty, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/kovalen.2021.v7.i3.15567

Abstract

The snake fruit peel (Salacca zalacca (Gaertn.) Voss) traditionally has several health benefits, one of which is to treat diabetes mellitus. This study aimed to determine the content of secondary metabolites in the ethanolic extract of snake fruit peel and its activity on glucose and urea creatinine levels in male white rats (Rattus norvegicus). Phytochemical screening of extracts was carried out qualitatively and quantitatively using UV-Vis Spectrophotometry. This study is a laboratory experimental study using 30 test animals divided into 6 treatment groups (normal control, negative control, positive control, 70 mg/kg BW, 140 mg/kg BW, and 280 mg/kg BW). The results showed that the ethanol extract of the bark of the salak fruit contained 7.61 %w/w alkaloids, flavonoids 0.041% w/w, tannins 1.18% w/w, and saponins 2% w/w. Ethanol extract of salak fruit peel dose of 140 mg/kg BW affected decreasing blood glucose and urea creatinine levels. The skin of the salak fruit has the potential to be further investigated as an antidiabetic.
Vitamin C Ikan Sidat (Anguilla Marmorata) Asal Sungai Palu dan Danau Poso Jamaluddin Jamaluddin; Agustinus Widodo; Anita Tri Damayanti
Ghidza: Jurnal Gizi dan Kesehatan Vol 2 No 1 (2018): July
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/ghidza.v2i1.2

Abstract

Pendahuluan & Tujuan: Ikan sidat (Anguilla marmorata (Q.) Gaimard) memiliki keunggulan gizi atau nutrisi yang tinggi seperti vitamin A, vitamin B, vitamin C, vitamin D, vitamin E, protein, mineral, dan asam lemak yang baik bagi kesehatan. Penelitian ini bertujuan untuk menentukan perbedaan kadar vitamin C ikan sidat (Anguilla marmorata (Q.) Gaimard) fase Glass eel, Yellow eel, dan Silver eel asal danau Poso dan muara sungai Palu. Bahan dan Metode: Pengujian kadar vitamin C menggunakan bahan berupa daging ikan sidat (Anguilla marmorata (Q.) Gaimard) fase Glass eel, Yellow eel, dan Silver eel dengan metode HPLC (High Performance Liquid Chromatography) dengan teknik Purposive Sampling yang berdasarkan ukuran, tempat dan fase ikan sebagai syarat untuk pengambilan sampel. Hasil: Ikan sidat (Anguilla marmorata (Q.) Gaimard) pada fase Glass eel, Yellow eel, dan Silver eel asal danau Poso dan muara sungai Palu tidak terdeteksi adanya kandungan vitamin C. Kesimpulan: Ikan sidat (Anguilla marmorata (Q.) Gaimard) tidak mengandung vitamin C
Vitamin A Ikan Sidat (Anguilla Marmorata) Asal Sungai Palu Dan Danau Poso Jamaluddin Jamaluddin; Agustinus Widodo; Nurul Mufliha
Ghidza: Jurnal Gizi dan Kesehatan Vol 2 No 1 (2018): July
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/ghidza.v2i1.4

Abstract

Pendahuluan & Tujuan: Ikan sidat (Anguilla marmorata) memiliki keunggulan gizi atau nutrisi yang tinggi seperti protein, mineral, asam lemak dan salah satunya yaitu vitamin A. Penelitian ini bertujuan untuk membandingkan jumlah kadar vitamin A ikan sidat (Anguilla marmorata) fase glass eel, yellow eel , silver eel asal Sungai Palu dan Danau Poso. Bahan dan Metode: Bahan yang digunakan dalam penelitian yaitu Ikan sidat (Anguilla marmorata) berukuran Glass eel, Yellow eel, Silver eel yang berasal dari Sungai Palu dan Danau Poso, Ethanol, KOH, n-Hexane, BHT (Butil Hidroksil Toluen), Methanol. Teknik pengambilan sampel menggunakan purposive sampling melihat berdasarkan jenis berat, ukuran dan lokasi pengambilan. Metode yang digunakan yaitu Kromatografi Cair Kinerja Tinggi (KCKT) dimana sebelumnya dilakukan uji saponifikasi (penyabunan) pada sampel ikan sidat. Hasil: Vitamin A pada ikan sidat asal Sungai Palu fase glass eel sebesar 4,01 mg/kg (1336,67 IU/100 gram), yellow eel sebesar 7,26 mg/kg (2420 IU/100 gram), silver eel sebesar 9,73 mg/kg (3243,34 IU/100 gram). Vitamin A pada ikan sidat asal Danau Poso fase glass eel sebesar 0,22 mg/kg (73,34 IU/100 gram), yellow eel 7,16 mg/kg (2386,67 IU/100 gram), silver eel sebesar 11,3 mg/kg (3766,67 IU/100 gram). Kesimpulan: Kadar vitamin A ikan sidat fase glass eel asal Sungai Palu dan Danau Poso berbeda signifikan.
Analisis Kandungan Vitamin B Pada Ikan Sidat (Anguilla Marmorata (Q.) Gaimard) Fase Elver Asal Danau Poso Fenti Fenti; Agustinus Widodo; Jamaluddin Jamaluddin
Ghidza: Jurnal Gizi dan Kesehatan Vol 2 No 2 (2018): December
Publisher : Universitas Tadulako

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/ghidza.v2i2.8

Abstract

Latar Belakang & Tujuan : Vitamin merupakan zat-zat organik kompleks yang diperlukan tubuh dalam jumlah yang kecil, umumnya tidak dapat disintetis oleh tubuh tetapi penting dalam proses metabolik salah satunya adalah vitamin B. Vitamin B terdiri dari vitamin B1, B2, B3, B6, B9 danB12. Sidat merupakan salah satu ikan yang memiliki kandungan nutrisi yang tinggi salah satunya vitamin B. Penelitian ini dilakukan untuk menentukan kadar vitamin B (B1, B2, B3, B6, B9, B12) pada ikan sidat (Anguilla marmorata (Q.) Gaimard) fase elver asal danau Poso . Bahan dan Metode : Penentuan kadar vitamin B1, B2, B3, B6, dan B9 menggunakan metode KCKT (Kromatografi Cair Kinerja Tinggi), dan pada vitamin B12 menggunakan metode LC-MS (Liquid Chromatography–Mass Spectrometry). Hasil : Vitamin B2 dengan kadar 0,133 mg/100g, vitamin B3 dengan kadar 1,895 mg/100g, dan vitamin B12 dengan kadar 0,017mg/100g sedangkan pada vitamin B1, B6, dan B9 tidak terdeteksi adanya kadar. Kesimpulan : Ikan sidat (Anguilla marmorata (Q.) Gaimard) fase elver asal danau Poso dapat dijadikan sumber vitamin B2, B3, dan B12.
Co-Authors A. Lasongke, Putri Faradila Abd. Rahman Razak Ade Fazliana Mantika Adrianton Adrianton Akhmad Khumaidi Amalia Purnama Zainal Amalia Purnamasari Zainal Amelia, Putri Anita Purwantari Anita Tri Damayanti Anugraini, Annisa Putri Arisca, Windhy Nur Armini Syamsidi Asty Ivon Samaliwu Bella Melinda Craig Proctor Djaleha, Helena Fransisca Dytha Okvhyanitha Ediati Sasmito Erdy Prasetyo Fayakun Satria Fenti Fenti Gugun Gunawan Ignatius T Hargiyatno Iklima Indriani, Meri Feki Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jamaluddin Jessica Jessica Joni Tandi Joni Tandi, Joni Khildah Khaerati Lilis Sadiyah M. Sulaiman Zubair Ma'rifah Ma'rifah Maskur, Risda Meita Meita Muhamad Rinaldhi Tandah Muhammad Sulaiman Zubair Musyahidah Musyahidah Nanang Fakhrudin, Nanang Natasha Setiawan Nur Pita Sari Nurlina Ibrahim Nurul Ambianti Nurul Mufliha Padhlun A Jerni Pitriani Rasida Risna Yanti Ritha Pratiwi Satriani Siti Nurhafsah Syaputra, Gunawan Syariful Anam Syariful Anam Syariful Anam Tien Wahyu Handayani Tunreng, Muh. Fadil Usman Wa Ode Sitti Musnina Wa Ode Sitti Musnina Wudianto Wudianto Yonelian Yuyun Yuliet Yuliet Yusriadi Yusriadi Zulfaidah