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Biocelebes
Published by Universitas Tadulako
ISSN : -     EISSN : 25805991     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 303 Documents
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Test Of The Effectiveness Of Root Alelopathic Extract (Cyperus rotundus L.) and Bandotan (Ageratum conyzoides L.) On The Growth Of Tomato Plants (Solanum lycopersicum L.) Dewi Arini; Wahyu Harso; Asri Pirade Paserang
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.796 KB) | DOI: 10.22487/bioceb.v13i3.14970

Abstract

Cyperus rotundus L. and Ageratum conyzoides L. are commonly found in tomato field as weeds species. Weed compete with the crop for nutrient, water and light. In addition, weed released allelopathy that inhibited the growth of crop. The aim of this study was to compare the ability of allelopathy from C. rotundus L. and Ageratum conyzoides L. root extract to inhibit tomato plant growth. The study was conducted in Completey Randomized Design with two factors. The first factor was root extract from either C. rotundus L. and A. conyzoides L. as allelopathy. The second factor was concentration of root extract from both weeds (100, 300 and 500 g/ml). Each treatment combination was repeated four times. The results showed that A. conyzoides L. root extract had higher inhibition to growth of tomato plant than C. rotundus L. root extract. Increasing root extract concentration from both weeds increased inhibition of tomato plant growth.
The Growth Of White Oyster Mushroom Mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) From Liquid And Solid Inoculum Nisfaun Safitriana; Umrah; Orryani Lambui
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (211.187 KB) | DOI: 10.22487/bioceb.v13i3.14971

Abstract

The study about the growth of white oyster mushroom mycelium (Pleurotus ostreatus) (Jacq) (P. Kumm) from liquid and solid inoculum conducted on January until July 2018 in Biotechnology laboratory, Biology Department, Faculty of Math and Science, Tadulako University. This study was aimed to find out the mycelium growth of source of stem inoculum and hood in liquid and solid media. This study was designed by Complete Randomized Design (CRD) which consisted of four treatments and five replications. The treatment were M1 (source of stem inoculum in liquid medium), M2 (source of stem inoculum in solid medium), M3 (source of hood inoculum in liquid medium), and M4 (source of hood inoculum in solid medium). The parameters were (a) incubation time till mycelium fills medium, (b) CFU, and (c) the viability of inoculum on producing medium. The result showed that the faster incubation were M1 and M3 (for 2 days, higher CFU was M3 with an average number 8,2 x 10 10 CFU /ml. The faster viability of mycelium growth were M3 with an average growth of mycelium 6,97 cm/days.
Formulation of Inoculum a white oyster Mushroom (Pleurotus ostreatus) on powder Khadijah; Umrah; Orryani Lambui
Biocelebes Vol. 13 No. 3 (2019)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (294.956 KB) | DOI: 10.22487/bioceb.v13i3.14972

Abstract

The study about the formulation of inoculum a white oyster mushroom (Pleurotus ostreatus) on flour. was conducted at Biotechnology laboratory of Departement , Faculty of Mathematic and Natural Science, Tadulako University. This study were aimed to oyster mushroom on production. on and to abridge the cycle of oyster mushroom production process of white.peplication used Compeletely Randomized Design (CRD) consist of six treatments and tree by comparing between (sawduts,rice,bran, and comflour) P1(50% : 27% : 23% , P2 (50% : 30% : 20%), P3 (50% : 33% : 17%), P4 (50% : 36% : 14% ), P5 (50% : 39% : 11%), P6 (50% : 42% : 8%). There are four parameters used in this study (1) .The growth of white oyster mushroom inoculum (2) the incubation time. and, (3) coloni forming unit (CFU), (4) Viability test .The resulth showed that the best growth mycelium was 1,3cm/days with incubation period was 26 days, the highest total of CFU in P5 was 12,7x 108 CFU/ml and was in P3 was 3,3 x 108 CFU/ml .The inoculum viability test of production media in P5, was faster in incubation period is 18 days.
PENINGKATAN PERTUMBUHAN TANAMAN JAGUNG ( Zea mays L.) MENGGUNAKAN JAMUR MIKORIZA ARBUSKULAR DARI JENIS YANG BERBEDA PADA KONDISI CEKAMAN AIR isna isna; Wahyu Harso; Yusran Yusran
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15081

Abstract

Arbsucular mycorrhizal fungi promote plant growth by enhancing mineral uptake. Contribution degree of arbuscular mycorrhizal fungi to promote plant growth depend on species of plant-fungus association. The aim of this study was to compare the ability of three species of Glomus to promote maize plant growth. Maize plants were inoculated with 20 g inoculum of either Glomus deserticola, Glomus etunicatum, or Glomus clorum. Inoculum was soil containing spore, hyphae and infected root. Maize plants without addition inoculum were also used as a control. Water availability in the soil as growing medium was maintained on 40% field capacity. The results showed that addition of inoculum from three species of Glomus increased average of maize plant shoot dry weight although there was no statisticaly significant differences. Maize plant inoculated with G. clorum had higher shoot dry weight than maize plant inoculated either with G. etunicatum or G. deserticola while root colonization by G. clorum was lowest.
PROFIL TOKSIKOLOGIS EKSTRAK DAUN TUMBUHAN BAKA-BAKA (Hyptis capitata Jacq.) PADA HATI TIKUS PUTIH (Rattus norvegicus) Sitti Ayu; Wahyu Harso; Maghfirahtul Jannah
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15082

Abstract

Hyptis capitata Jacq. plant has been using for traditional medicine. Utilization of medicinal plant must always consider to given dose because of toxic effect when too much medicine is taken. The aim of this study was to measure the liver damage in rats caused by H. capitata Jacq. leave extract. Either 300 (P1), 600 (P2) or 900 (P3) µg/kgBW H. capitata Jacq. leave extract was given orally to rats every 24 hours during 14 days. Zero point five ml ethanol 96% was given daily (K-) and without given anything (K+) was also conducted as a control. Both macroscopic and microscopic of liver damage were assessed. The result showed that rats given P3 treatment had the highest liver damage. The liver damage in rats was not statistically significant difference between P3 and K- treatments. The lowest liver damage was in rats given K+ treatment. There was no significant difference between P1 and P2 treatments on rats liver damage. Utilization of medicinal plant as traditional medicine should always be consider to doses.
UJI KEEFEKTIFAN EKSTRAK ALELOPATI AKAR TEKI (Cyperus rotundus L.) DAN BANDOTAN (Ageratum conyzoides L.) TERHADAP PERTUMBUHAN SAWI HIJAU (Brassica rapa L.) Sri Rahayu; Asri Pirade Paserang; Wahyu Harso
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15083

Abstract

Weeds such as Cyperus rotundus L. dan Ageratum conyzoides L. release allelopatic compounds which can inhibit the growth of crops. The aim of this study was to compare the inhibition of allelopatic compounds contained C. rotundus L. and A. conyzoides root exctract to Brassica rapa L. growth. This study was conducted in a greenhouse and was based on Completely Randomized Design with two factors. The first factor was type of root extracts (extract from either C. rotundus root or A. conyzoides root) and the second factor was concentration of root extract. Root extract concentrations added to growth medium of B. rapa L. 10 days after planting were 0, 100, 300 and 500 g/L respectifely. The addition of root extract was repeated every week until crop harvested. The results showed that there was not significantly different between C. rotundus and A. conyzoides roots extract to inhibit B. rapa growth. Increassing of root extract concentrations from both roots increased inhibition of B. rapa growth.
PENGARUH INTENSITAS CAHAYA MATAHARI DANKETERSEDIAAN AIR TERHADAP PERTUMBUHANTANAMAN CABAI RAWIT (Capsicum frutescens L.) Ajis Ajis; Wahyu Harso
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15084

Abstract

Light intensity is one the most important factor to the growth of Capsicum frutescens L, however high light intensity can cause high evaporation which causes plant suffering from drought. The aim of this study was to determine the effect of different sunlight intensities and soilwater availabity to C. frutescens growth. The study was conducted by a completely randomized design method with two factors. The first factor was light entensity which consisted of 100 and 50% sunlight intensity (4287 and 2587 lux). The second factor was soil water avaibility which consisted of 75, 50 and 25% fied capacity. The results showed that plants grown at 100% light intensity had higher growth than plants grown at 50% light intensity on every soil water availability treatments. Soil water availability was not significantly affecting plant growth. It might be caused by plant received short period of sunlight.
FORMULASI MEDIA PERTUMBUHAN MISELIUM JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq) P. Kumm) DENGAN SUPLEMENTASI LIMBAH SABUT KELAPA Sitti Masyitah; Umrah Umrah
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15085

Abstract

The research entitled Formulation of media for growth of white oyster mushroom mycelium ((Pleurotus ostreatus (Jacq) P. Kumm) with waste of coconut fiber supplementation was carried out out in Biotechnology Laboratory unit, Department of Biology, Faculty of Mathematics and Natural Sciences. This research purposes; (a) To find out the formulation of coconut fiber waste as a supplementary medium for growing white oyster mushrooms (P.ostreatus); (b) to find out the formulation at the maximum dose of coconut husk supplementation in the medium for good growth of white oyster mushrooms (P.ostreatus). This study was designed based on a Completely Randomized Design (CRD) consisting of 7 settings and 4 replications. The help arrangement is given between Sawdust: Rice Bran: Lime: Coconut Fiber Waste. Namely M0 (Basic media 100%, without Supplements), M1 Basic media 95% + Supplements 5%, M2 (Basic media 90% + Supplements 10%), M3 (Basic media 85% + Supplements 15%), M4 (Basic media 80% + Supplement 20%), M5 (Basic media 75% + Supplement 25%), M6 (Basic media 70% + Supplement 30%). Observation parameters; (a) prohibiting mycelium; (B) Incubation time; (c) Number of colonies (Colony Forming Units); (d) Macroscopic and microscopic observations of mycelium. The results showed that the fastest mycelium growth in M6 treatment was 1.193 cm and the lowest was M0 treatment which was 1.123 cm. The lowest incubation time at M6 is 28 days and the lowest at M0 is 44 days. The highest number of colonies (CFU) in M6 treatment was 1.96 x 10 -12 CFU / g and the lowest was maintenance of M3 1.03 x 10-12 CFU / g.
UJI BEBERAPA FORMULA INOKULUM TERHADAP PRODUKSI TUBUH BUAH JAMUR TIRAM PUTIH (Pleurotus ostreatus (Jacq.) P.Kummer.) Dana Sulistia; Umrah Umrah; I Nengah Suwastika
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15086

Abstract

The study of test for several inoculum formulas on the production of white oyster mushroom body (Pleurotus ostreatus (Jacq.) P.Kummer.) conducted from April to September 2018 at Biotechnology laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Tadulako University. This study were aimed to determine whether the treatment of the inoculum formula tested on the production medium can form the white oyster mushroom fruit body and to find out which inoculum formula can form the optimum fruit body in the production medium. This study was conducted by Randomized Complete Design (RAL) consisted of 4 treatments and four replications, I1 (100% corn seeds), I2 (50% : 23%: 27%), I3 (50% : 17% : 33%) dan I4 (95% : 5%). The results showed that I 3 was the best inoculum for the growth of the white oyster mushroom fruit body compared to the other inoculums which were characterized by a wide mushroom hood, the length was long and the weight of fresh mushroom was high. The inoculum formula and the best dosage for body production of white oyster mushroom are found in treatment I3 (50% sawdust + 17% corn flour + 33% bran).
FORMULASI MEDIA PRODUKSI JAMUR TIRAM (Pleurotus ostreatus) DENGAN SUPLEMENTASI AMPAS SAGU Sri Utami; Umrah umrah; I Nengah Suwastika
Biocelebes Vol. 14 No. 1 (2020)
Publisher : Biology Department, Mathematics and natural science, Tadulako University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22487/bioceb.v14i1.15087

Abstract

This study aims to know the basic media with sago pulp supplementation which can be formulated into a medium for producing white oyster mushrooms and also to know the comparison of the right media and supplements in the formulation to give maximum oyster mushrooms production. In this study using a comparison with sawdust substrate and sago pulp to see how effective the sago pulp substrate as a supplement media for the oyster mushroom production. the program used is experimental designed in Completely Randomized Design (CRD), consists of three replications so that twelve experiments were obtained. The treatment arrangement is M1: Base media without supplements (controls), M2: 90% base media + 10% sago pulp, M3: 80% basic media + 20% sago pulp, M4: base media 70% + sago pulp 30%. The observation parameters used in the study are mycelium growth, fruit body growth, biological efficiency and measurement of protein levels in the fruit body of the oyster mushroom. The results showed that the addition of sago pulp in the media of oyster mushroom production had no effect on the growth of oyster mushrooms. This can be seen from the media of production of M1 (without the addition of sago pulp) experiencing a higher growth rate of mycelium compared to other treatments. The addition of sago pulp has an effect on the levels of oyster mushroom protein. This can be seen from the protein content of oyster mushrooms in M2 production media which have high protein content. Normal levels of white oyster mushrooms in sawdust media range from 18% -20%. This is due to the mixing of wood powder as a production medium and 10% sago pulp as supplementation to produce more nutrient levels.

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