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INDONESIA
ODONTO Dental Journal
Published by Universitas Islam Sultan Agung
ISSN : -     EISSN : -     DOI : -
Core Subject : Health,
Medicine & Pharmacology
Arjuna Subject : -
Articles 12 Documents
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Search results for , issue "Vol 4, No 2 (2017): December 2017" : 12 Documents clear
UJI AKTIVITAS ANTIOKSIDAN EKSTRAK BAWANG PUTIH (Allium sativum) Djuned Prasonto; Eriska Riyanti; Meirina Gartika
ODONTO : Dental Journal Vol 4, No 2 (2017): December 2017
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.4.2.122-128

Abstract

Background: Organosulfur compound, allicin and phenolic are the main compound in garlic responsible for antioxidant activity. Correlation between free radicals and some dental diseases, such as free radicals and Reactive Oxygen Species (ROS) responsible for periodontal inflammation, caries, lesions and oral cancers. The research objective is to get scientifc data about the antioxidant activity from garlic extract (Allium sativum).Method: The research was conducted using three different varieties of garlic:local garlic varieties Ciwidey, single local garlic’s clove, and import garlic which is found at the market in Bandung. Garlic extract is made by maceration using ethanol 96% and antioxidant activity test using DPPH method (1,1-diphenyl-2- picrylhydrazyl). Statistical analysis using One Way ANOVA followed by post hoc analysisResult: three varieties of garlic have a strong antioxidant activity. Value of IC50 = 13.61 mg / ml for local garlic varieties Ciwidey, IC50 = 10.61 mg/ml for single local garlic’s clove and IC50 = 11.32 mg/ml for imports garlic.Conclusion: Three varieties of garlic have different antioxidant strength and the best antioxidant strength is single local garlic’s clove.
STIMULASI EKSTRAK PROPOLIS PADA ODONTOBLAST LIKE CELLS YANG DIINDUKSI LACTOBACILLUS ACIDOPHILUS INAKTIF TERHADAP EKSPRESI TLR2 DAN TNFα Ira Widjiastuti; Nadia Irnatari; Mandojo Rukmo
ODONTO : Dental Journal Vol 4, No 2 (2017): December 2017
Publisher : Faculty of Dentistry, Universitas Islam Sultan Agung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30659/odj.4.2.85-93

Abstract

Background: Lactobacillus acidophilus, Gram-positive bacterias that enterthe dentinal tissue during the carious process are suspected to influence the immune response in human dental pulp. Odontoblasts situated at the pulp dentin interface are the frst cells encountered by these bacteria and have an important role in this response. Lipoteichoic acid (LTA), a wall component of Gram-positive bacteria, triggered the activation of the odontoblasts. LTA upregulated the expression of its own receptor TLR2, as well as the production of proinflammatory cytokineTNFα. Propolis is a resinous material that holds a great potential as an antiinflammatory agent. Present studies have shown that propolis has a reduction effect towards the proinflammatory cytokines expression and favor pulp healing.Purpose: To reveal the moleculer mechanism of propolis stimulation onodontoblast like cells, induced by inactive Lactobacillus acidophilus.Methods: This review was presented in odontoblasts like cells culture induced by inactive Lactobacillus acidophilus and exposed to propolis extract. Pulp cell culture isolated from human impacted thirds molar that has been extracted. Observation and measurement the expression of TLR2 and TNFα was processed by using immunocytochemistry (ICC) technic.Result: Data analysis with ANOVA test, a signifcant difference in every group(p<0,05) was present. The expression of TLR2 and TNFα were shown at lowlevel percentation on odontoblast like cells, induced by Lactobacillus acidophilus and propolis extract stimulation.Conclusion: Propolis extract stimulations lower the TLR2 and TNFα expression on odontoblast like cells, induced by inactive Lactobacillus acidophilus

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