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Biopropal Industri

biopropal
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Published by Kementerian Perindustrian Republik Indonesia

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Core Subject : Agriculture, Engineering,

Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

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Articles 208 Documents

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Pertumbuhan Bakteri Laut Shewanella indica LBF-1-0076 dalam Naftalena dan Deteksi Gen Naftalena Dioksigenase - (The Growth of Marine Bacteria Shewanella indica LBF-1-0076 in Naphthalene and Naphthalene dioxygenase Gene Detection) Nuzul Farini; Ahmad Thontowi; Elvi Yetti; Suryani Suryani; Yopi Yopi
Biopropal Industri Vol 8, No 1 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Crude oil exploitation which often occured offshore can cause water pollution in the sea since its contains naphthalene which is a hazardous compounds. This research used marine bacteria LBF-1-0076 that have ability in naphthalene degradation. This research aimed to study the parameter effect of naphthalene and cell concentration toward marine bacteria LBF-1-0076. This research also identified isolate LBF-1-0076 and detected the encode gene of naphthalene dioxygenase. Based on growth test result, the optimum naphthalene degradationby isolate LBF-1-0076 occured in 75 ppm naphthalene concentration with 15cell concentration. The result of 16S rDNA gene analysis showed that LBF-1-0076 was identified as Shewanella indica strain 0102 with identical value 99%. The result of naphthalene dioxygenase gene detection using Polymerase Chain Reaction (PCR) showed that the isolate contained naphthalene dioxygenase gene with size ±377 bp. Therefore, LBF-1-0076 potential as bioremediation agent to solve crude oil contamination in the sea.Keywords: crude oil, marine bacteria, naphthalene, naphthalene dioxygenase, Shewanella indicaABSTRAKEksploitasi minyak bumi yang sering terjadi di laut mengakibatkan adanya pencemaran minyak di laut. Naftalena merupakan salah satu senyawa dominan berbahaya yang terkandung dalam minyak bumi dan dapat mengakibatkan pencemaran perairan. Penelitian ini menggunakan bakteri laut LBF-1-0076 yang memiliki kemampuan untuk mendegradasi naftalena. Tujuan dari penelitian ini adalah mempelajari pengaruh parameter konsentrasi naftalena dan konsentrasi sel terhadap bakteri laut pendegradasi naftalena LBF-1-0076. Penelitian ini juga bertujuan untuk mengidentifikasi isolat LBF-1-0076 dan mendeteksi gen pengkode naftalena dioksigenase. Berdasarkan hasil uji pertumbuhan, degradasi naftalena yang optimal oleh isolat LBF-1-0076 terjadi pada konsentrasi naftalena 75 ppm dengan konsentrasi sel 15. Hasil analisis gen 16S rDNA menunjukkan isolat LBF-1-0076 teridentifikasi sebagai Shewanella indica strain 0102 dengan nilai keidentikan 99%. Hasil deteksi gen naftalena dioksigenase dengan menggunakan Polymerase Chain Reaction (PCR) menunjukkan bahwa isolat tersebut mempunyai gen naftalena dioksigenase dengan ukuran ±377 bp. Oleh karena itu, isolat LBF-1-076 berpotensi sebagai agen bioremediasi untuk mengatasi masalah pencemaran minyak bumi di laut.Kata kunci: bakteri laut, minyak bumi, naftalena, naftalena dioksigenase, Shewanella indica

Characteristics of Tanning Leather Using Gambir on pH 4 and 8 Ardinal Ardinal; Anwar Kasim; Sri Mutiar
Biopropal Industri Vol 4, No 2 (2013)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Tannery production process in Indonesia is still using chromium sulfate or mimosa in general. Gambier (Uncaria gambier Roxb.) extract could be used as material for tanners because it contains tannin. This study aimed to investigate the characteristics of tanned leather using gambier solution at pH 4 and 8. Standard tanning method was used which includes the processing of goat leather with salt, acid and then tanned with gambier extracts. Observation of tanned leather refers to the SNI-06-0463-1989-A and ISO 0234:2009. The results showed that characteristics of tanned leather with solution of gambier at pH 4 and 8 respectively i.e.: degrees of tanning 29.87% and 39.55%, tensile strength 279.94 kg/cm2 and 433.85 kg/cm2, leather conditions was limp, light gray colour and yellow, thickness of 0.12 mm and 0.11 mm, and density 0.74 g/cm3 and 0.74 g/cm3. Tanned leather from different pH conditions generally has the same characteristic and meet standards.

The Effect of Filler on the Quality of Snack Food Extruded from Solid Matter of Industrial Soybean Tofu Farid Salahudin; Syamsixman Syamsixman
Biopropal Industri Vol 1, No 2 (2010)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Tofu filter cake is the waste of tofu processing which is used for animal feed. In the other hand tofu filter cake can be used at extruded food processing. The purpose of this study is to look for the formulas and filler materials which could produce extruded food. The first variable in this research is the kind of corn that is pop corn and maize corn. The second variable is formulas of filler material that is composition of corn: rice: tofu filter cake = 1:1:0.5; 1:1:0.25; 1:2:0.5 and 1:2:0.25. The result is the formula which can produce best extruded food is pop corn: rice: tofu filter cake = 1:2:0.25 with Expansion Ratio (ER) 2.22 and protein content 8.83%.

Low Lactose Milk Production of Soybean by Fermentation Technique Using Rhizopus oligosporus Farid Salahudin; Hidayati Hidayati
Biopropal Industri Vol 4, No 1 (2013)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Milk is an important food for baby that contains lactose. Normally, a baby could produce lactase enzyme that digest lactose, but in the diarrhea case lactose could not be digested. So, Low Lactose Milk is needed. Low Lactose Milk usually produced from rice or almonds that have low protein. Soybean (Glycine max) is the commodity with rich of protein and also contains raffinose and stachyose, which can lead flatulence. Raffinose and stachyose could be reduced by Rhizopus oryzae at tempe process from lamtoro beans. So the aim in this research is to know the optimum time of soybean fermentation with R. oryzae to reduce stachyiose and raffinose. The research was done with innoculation of R. oryzae isolate in the soybeans fermentation for 72 hours. N index, raffinose and stachyose level was tested. The result shows that optimum fermentation time is 48 hour and using 5% skim milk as filler.

EVALUASI KERAGAMAN GENETIK SEMBILAN VARIETAS RAMBUTAN (Nephelium lappaceum) DENGAN MARKA RAPD - (Evaluation of Nine Rambutan (Nephelium lappaceum) Varieties Genetic Diversity Using RAPD Markers) Yuliana Galih Dyan Anggraheni; Enung Sri Mulyaningsih
Biopropal Industri Vol 9, No 1 (2018)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

High diversity of rambutan varieties is potensial to develop new improved rambutan varieties. Due to limitation of rambutan genetic diversity information, the usage of RAPD (Random Amplified Polymorphic DNA) markers becomes an efficient option. This research aimed to study the diversity of rambutan from the collection of LIPI germplasm garden genotypically. Nine rambutan varieties were analyzed using six RAPD markers which generated 45 bands. Polymorphism analysis showed variation between varieties 56,6% and PIC (Polymorphic Information Content) value 0,2-0,5. UPGMA (Unweighted Pair Group Method Arithmetic) dendogram divided nine rambutan varieties in two groups with similarity index of 85-95%. First group was Si Nyonya varieties and the second group consist of Rapiah, Aceh Lebak, Unidentified, Binjai, Parakan, Si Macan, Aceh Pagar and Lebak Bulus varieties. The group differences allegedly related to the stickiness of the flesh to the seed. Aceh Lebak varieties has the closest genetic relationship to unidentified varieties of 95%.Keywords: genetic diversity, molecular marker, phylogenetic tree, PIC value, rambutanABSTRAKTingginya keragaman varietas rambutan menjadi salah satu potensi untuk mengembangkan varietas unggul baru. Akan tetapi, ketersediaan informasi keragaman genetik rambutan masih terbatas. Penggunaan teknik marka RAPD (Random Amplified Polymorphic DNA) menjadi pilihan untuk analisis keragaman genetik tanaman karena cepat, mudah dan efisien. Tujuan penelitian ini adalah untuk mempelajari keragaman rambutan koleksi KPN (Kebun Plasma Nutfah) LIPI secara genotipik. Sembilan varietas rambutan digunakan dalam penelitian ini. Hasil analisis dengan menggunakan enam marka RAPD menghasilkan total pita 45. Hasil analisis polimorfisme menunjukkan keragaman antar varietas sebesar 56,6% dan nilai PIC (Polymorphic Information Content) sebesar 0,2-0,5. Dendogram UPGMA (Unweighted Pair Group Methode Arithmetic) membagi sembilan varietas rambutan dalam dua kelompok dengan indeks kesamaan sebesar 85-95%. Kelompok satu merupakan jenis varietas Si Nyonya sedangkan kelompok kedua terdiri dari varietas Rapiah, Aceh Lebak, Unidentified, Binjai, Parakan, Si Macan, Aceh Pagar dan Lebak Bulus. Perbedaan kelompok ini diduga berkaitan dengan sifat kelekatan daging buah terhadap biji. Varietas Aceh Lebak memiliki kekerabatan paling dekat dengan varietas unidentified sebesar 95%.Kata kunci: keragaman genetik, nilai PIC, penanda molekuler, pohon filogenetik, rambutan

Pengolahan Limbah Perendaman Karet Rakyat dengan Metode Koagulasi dan Flokulasi Menggunakan Aluminium SUlfat, Ferri Klorida, dan Poli Aluminium Klorida (PAC) Riskawanti Suwandi; Chairul Irawan; Andri Taruna Rachmadi
Biopropal Industri Vol 7, No 1 (2016)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Rubber is one of important commodities in Central Kalimantan with more than 220.000 tons productions in 2013. However, the rubber immersion wastewater which has been discharged into the water body becomes problem. This research aims to know the effect of Al2(SO4)3, FeCl3 and PAC as coagulant in doses 2 g/L, 4 g/L and 8 g/L to reduce COD, BOD5, TSS, TDS and color concentration of the wastewater. Jar-Test was used with rapid mixing 200 rpm for 3 minute and slow mixing 50 rpm for 10 minute then settlled for 1 hour. The results showed that PAC with the dose of 4 g/L was better in reducing the COD, BOD5 and TSS than FeCl3 and Al2(SO4)3. After coagulation-flocculation, COD, BOD5 and TSS are 37,07 mg/L, 14,29 mg/L and 24,00 mg/L, respectively. On the other hand, TDS and color value showed higher concentration than standard with 514,00 mg/L and 106,00 PtCo, respectively.Keywords: Al2(SO4)3, coagulation-flocculation, FeCl3, PAC, rubber immersion wastewater ABSTRAKKaret merupakan salah satu komoditas penting di Provinsi Kalimantan Tengah dengan total produksi lebih dari 220.000 ton di tahun 2013. Permasalahan dalam produksi karet rakyat adalah limbah cair dari perendaman karet yang biasanya dibuang langsung ke badan air. Salah satu metode pengolahan limbah cair perendaman karet adalah dengan proses koagulasi-flokulasi. Penelitian ini dilakukan untuk mengetahui pengaruh jenis dan dosis koagulan Al2(SO4)3, FeCl3 dan PAC dengan dosis masing-masing sebesar 2 g/L, 4 g/L dan 8 g/L terhadap proses pengolahan limbah perendaman karet dengan metode koagulasi-flokulasi berdasarkan parameter COD, BOD5, TSS, TDS dan warna sebelum dan sesudah pengolahan. Penelitian ini dilakukan dengan menggunakan Jar-Test pada pengadukan cepat 200 rpm selama 3 menit, dilanjutkan pada pengadukan lambat 50 rpm selama 10 menit dan pengendapan selama 1 jam. Berdasarkan hasil penelitian diperoleh hasil bahwa PAC lebih baik dalam mereduksi konsentrasi COD, BOD5, TSS, TDS dan warna. Dosis yang disarankan berdasarkan penelitian ini sebesar 4 g/L. Konsentrasi COD, BOD5, TSS, TDS dan warna limbah perendaman karet sebelum proses koagulasi dan flokulasi belum memenuhi baku mutu Keputusan Menteri Negara Lingkungan Hidup No. KEP-51/MENLH/10/1995. Setelah diolah, didapatkan konsentrasi COD, BOD5 dan TSS berturut-turut sebesar 37,07 mg/L, 14,29 mg/L dan 24,00 mg/L yang telah memenuhi standar baku mutu limbah cair karet tetapi pada konsentrasi TDS 514,00 mg/L dan warna 106,00 PtCO masih belum memenuhi standar baku mutu limbah cair karet sehingga perlu pengolahan lebih lanjut sebelum dibuang.Kata kunci: Al2(SO4)3, FeCl3, koagulasi-flokulasi, limbah perendaman karet, PAC

The Application of Encapsulated Gluten on Tapioca Wet Noodle Making Processing Husniati Husniati; Siti Nurdjanah; Ryan Prakasa
Biopropal Industri Vol 6, No 1 (2015)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Gluten is a specific protein found in wheat and functionally needed to improve its elasticity and texture of carbohydrate-based dough. Gluten, isolated from wheat, can be used in the free-form or encapsulation. The aim of this research was to investigate the effects of encapsulated gluten addition on properties of tapioca for wet noodle production. The parameters studied were texture, sensory, and pasting properties of the noodles. The ratios of encapsulated gluten to tapioca were 97%:3%, 96%:4% and 95%:5%. The results showed that the use of encapsulated gluten as much as 3% w/w gave the best noodle with the characteristics of setback viscosity, low cooking loss and organoleptically preferred by panelists.

The Ability of Kayambang (Salvinia molesta D.S Mitchell) and EcEng Gondok (Eichornis crassipies (Mart.) Solms) to Decrease the Nitrogen Degree of liquid Waste from PTPN XIII Palm Oil factory in Ngabang-West Kalimantan Agus Sri Mulyono; Dessy Sudilawati
Biopropal Industri Vol 1, No 1 (2010)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

The wastewater effluent of Ngabang Palm Oil Industry in one of several industrial waste, that contains high organic substances. One of them is nitrogen (N-total). Waste water treatment of palm oil industry used ponding system today, and one of solution to treat that wastewater effluent is using aquatic weeds as biofilter action. Water hyacinth (Eichornia crassipes (Mart.) Solms) and water fern (Salvinia molesta D.S.Mitchell) known as aquatic weeds that capable to decrease in a large amount the organic and inorganic substances. The aim of this research is to study the capacity of water hyacinth and water fern in decreasing nitrogen compounds (N-total, ammonia, nitrate, nitrite, N-organic) in wastewater effluent palm oil industry based in variation of treatment and difference of exposure time. This research was carried out in green house and Biology Laboratory of Biology Programme Study, Mathematic and Science Faculty of Tanjungpura University and Testing Laboratory of Institute for Industrial Research And Standardization Pontianak, from July 1st – October 31th, 2006. This experiment was designed in Completely Randomized Factorial Design with three treatments and three replication times. The collected data analized by Annava Test and least Significance Difference (LSD) test. The result show that the treatment with combination of water hyacinth and water fern were able to decrease the N-total (92,64%); ammonia (96,47%); and nitrite (90,14%).

SEPARATION OF FERMENTED INULIN FIBER BY Lactobacillus acidophillus USING Aspergillus clavatus-CBS5 THROUGH MICROFILTRATION MEMBRANE - (PEMISAHAN SERAT INULIN TERFERMENTASI OLEH L. acidophillus MENGGUNAKAN A.. clavatus-CBS5 MELALUI MIKROFILTRASI MEMBRAN) Aspiyanto Aspiyanto; Agustine Susilowati; Yati Maryati; Hakiki Melanie
Biopropal Industri Vol 8, No 2 (2017)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

The condition of Stirred Filtration Cell (SFC) was used as reference to a large-scale process conditions (modules) on the separation offermented inulin fibers by Lactobacillus acidophillus. Inulin hydrolyzate as biomass was produced from inulinase hydrolysis stage by inulinase enzyme from Aspergillus clavatus-CBS5. Separation of inulinfiber aims to obtain inulin fiber through a microfiltration (MF) membrane 0.45 μm at room temperature, 400 rpm stirrer cycle and 40 psia for 0, 30, 60, 90 and 120 min. The results showed that best separation time was 120 minutes based on optimal CBC (cholesterol binding capacity) which fermented inulin fiber concentrate was produced with a total sugar concentration of 105.21 mg/mL, total solids 2.11%, total fiber 23.36%, total acid 6.66% (dry weight), 4.05 mg of dissolved protein/mL and CBC 13.781 mg/g. MF membrane increased the CBC by 23.4% compared to no separation process.Keywords: cholesterol binding capacity, inulin fiber, microfiltration membrane, permeate, retentateABSTRAKKondisi Sel Filtrasi Berpengaduk (SFB) digunakan sebagai acuan menuju kondisi proses skala besar (modul) terhadap pemisahan serat inulin terfermentasi oleh Lactobacillus acidophillus. Hidrolisat inulin yang digunakan sebagai biomassa dihasilkan dari tahapan hidrolisa inulin oleh enzim inulinase dari kapang Aspergillus clavatus-CBS5. Pemisahan serat inulin bertujuan untuk mendapatkan serat inulin melalui membran mikrofiltrasi (MF) 0,45 µm pada temperatur ruang, kecepatan putaran pengaduk 400 rpm dan tekanan 40 psia selama 0, 30, 60, 90 dan 120 menit. Hasil penelitian menunjukkan bahwa waktu pemisahan yang lama dapat menahan dan meningkatkan padatan total, serat total, asam total, protein terlarut dan kemampuan pengikat kolesterol (KPK) tetapi menurunkan gula total dalam retentat. Membran mikrofiltrasi melewatkan dan menurunkan gula total, protein terlarut dan KPK tetapi meningkatkan padatan total, serat total, asam total dalam permeat. Berdasarkan KPK optimal, waktu pemisahan terbaik dicapai setelah 120 menit. Pada kondisi ini dihasilkan konsentrat serat inulin terfermentasi dengan konsentrasi gula total 105,21 mg/mL, padatan total 2,11%, serat total 23,36%, asam total 6,66% (berat kering), protein terlarut 4,05 mg/mL dan KPK 13,781 mg/g. Membran MF mampu meningkatkan KPK 23,4% dibandingkan tanpa menggunakan proses pemisahan.Kata kunci: kemampuan pengikat kolesterol, membran mikrofiltrasi, permeat, retentat, serat inulin

Zinc Adsorption Kinetics Using Pseudomonas as Biomass Hidayati Hidayat; Yoyon Suyono
Biopropal Industri Vol 5, No 1 (2014)
Publisher : Balai Riset dan Standardisasi Industri Pontianak

Zinc adsorption kinetics using Pseudomonas aeruginosa and Pseudomonas sp. was conducted to determine the ability of biomass to adsorb Zinc, and also to get the biosorption equilibrium and kinetics. Adsorption process conducted in this study was using batch method with variables on the amount of biomass (0.01 g, 0.02 g, 0.03 g, 0.04 g and 0.05 g), adsorption time (30, 60 and 120 min) and pH (4 and 6). Zinc metal biosorption kinetics following the pseudo-second-order equation. The highest correlation coefficient obtained on the condition of 0.04 g biomass for both pH 4 and pH 6 was 0.999. The best Zinc metal adsorption equilibrium models obtained at pH 4 with contact time at 60 minutes. This heavy metal adsorption equilibrium models comply the Freundlich isothermal with a correlation coefficient (R2) of 0.973.

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