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Penelitian Pendahuluan Pengaruh Daun Manggis sebagai Rodentisida Nabati pada Mencit Mus musculus Strain Balepsi
Agus Kardinan;
Momo Iskandar;
Mukhasim Mukhasim
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada
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DOI: 10.22146/jpti.12409
Research has been conducted at Pest and Disease Laboratory, Research Institute for Spices and Medicinal Crops, Bogor, during 1997-1998. The objective was to evaluate the possibility of Mangosteen (Garcinia mangostana) leaves to be used as botanical rodenticide which is tested to reproduction ability of mice Mus musculus. Eight to ten weeks old mice with the average weight of 25 gram were obtained from Research Institute for Livestock, Ciawi. Research was arranged at randomized block design, 8 treatments and 4 replications. Data was analyzed by determining the average value and their standard deviation values. Observations were done to the weight of testis, weight of embryo, number of embryo, volume of extracts drank by mice and mice behavior during experiment. Result revealed that there was no indication that the leaves extract affected the weight of testis, but it affected the number and weight of embryo. There was indication that mangosteen leaves acted as antifertility on mice. Giving leaves extract increased the total volume of extract drink. There was no abnormality at the activity of mice during experiment, included sex activity, poisonous symptoms and others.
Pengendalian Diaphorina citri (Vektor Penyakit CVPD) dengan Metarrhizium anisopliae
Kardi Raharjo;
Susamto Somowiyarjo;
F. X. Wagiman
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada
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DOI: 10.22146/jpti.12404
CVPD is transmitted by Diaphorina citri. Measures to control D. citri by using biological controlling agents have opportunity to reduce insecticide application. The objectives of the research are: to measure effectiveness of Metarrhiziurn anisopliae (Metch.) Sorok. in controlling D. citri, effect fructose and time application namely before and after insect infestation. The first phase of the research phase has been conducted in Temanggung, Completely Randomize Design (CRD) factorial with three time replication. Factor I: sterile water without fructose, concentration 10^6 conidia/ml without fructose, concentration 10^8 conidia/ml without fructose, concentration 10^10 conidia/ml: without fructose, sterile water + fructose 5 mg/ml, concentration 10^6 conidia/ml without fructose 5 mg/ml, concentration 10^8 conidia/ml + fructose (fungi application before insect infestation) and W1 (fungi application after insect infestation). Research phase II was carried out with the best treatment combination compare with control treatment in Temanggung and Bantul. The results of experiment showed that the initial die of D. citri caused by M. anisopliae infection are on 4-6 days after application. The application of M. anisopliae at concentration 10^10 conidia/ml without fructose, applied after insect infestation was most effective. The application after insect infestation was more effective compare with application before insect infestation especially on 4 days after application, but on 35th days after application there was no significant difference. Fructose has no effect to mortality of D. citri.
Beberapa Catatan Mengenai Hama Baru: Penggulung Daun Teh Siput Tanpa Cangkang, Parmarion pupillaris
Bambang Rahayu T. P.;
Siwi Indarti;
Tri Harjaka
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada
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DOI: 10.22146/jpti.12410
In the past tea plantation at Pagilaran estate is attacked by leaf roller pests. These are Homona coffearia, Cydia leucostoma, and Gracilaria theifora. At present a slug Parmarion pupillaris is found and considered as a new leaf roller pest, because they live inside of the rolled leaf. This symptom is not known before. The population of Parmarion slug is high 3-4 slugs per bush of tea at the sub-division of Pagilaran estate of Pagilaran, Binorong and Garjito. This condition maybe supported by the weather that Pagilaran estate have a high rainfall of 4,842-6,067 mm per year with only 1-2 months of dry season.
The Role of Extracellular Protein on the Pathogenicity of Xanthomonas campestris pv. citri
Tri Joko;
Siti Subandiyah;
Susamto Somowiyarjo
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada
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DOI: 10.22146/jpti.12405
A research on the pathogenicity of Xanthomonas campestris pv. citri, the causal agent of citrus canker, has been carried out to study the growth characteristics of the pathogen on some media, physiological characteristics, and the role of extracellular protein on the bacterial pathogenicity. Extracellular protein of X. campestris pv. citri was extracted using ammonium sulfate precipitation. The extracted protein samples were electrophoresed on 10% polyacrilamide gel in the presence of sodium dodecyl sulfate at 15 mA/150 V for 1.5-2 hrs. Pathogenicity assay was conducted by infiltration of bacterial cell and extracellular protein suspension into citrus leaf tissues. The results showed that X. campestris pv. citri was able to grow well on all media. It possess specific protein with molecular weight of 25.71 KDa. Bacterial cell and extracellular protein of X. campestris pv. citri were able to produce typical symptoms of canker, while other closely related Xanthomonas campestris pathovars were only able to produce hypersensitive reaction on citrus leaves.
Analisis Runutan Nukleotida Gen Protein Selubung Dua Strain Peanut Stripe Virus
Hasriadi Mat Akin
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada
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DOI: 10.22146/jpti.12406
Nucleotide sequence analysis of coat protein gene from two biological strains of peanut stripe virus (PStV), severe blotch (PStV-Isb) and stripe (PStV-Is), has been done to study molecular variation of these strains. Comparison of predicted amino acid sequence of PStV coat protein with other members of bean common mosaic virus (BCMV) subgroup was also carried out to study the taxonomic: relationship between PStV and BCMV subgroup. The CP gene from these strains contained 861 nucleotides (nts) and encoded 287 amino acids. There were seven nucleotides of the CP gene and one nucleotide of NIb cistron that were different between PStV-Is and PStV-Isb. However, only one nucleotide at position 9309 of the CP gene caused differential amino acid residue at position 49 of the coat protein, methionine (M) for PStV-Isb and isoleucine (I) for PStV-Is. Comparison of the ammo acid sequences revealed a sequence similarity of 89,9-90,9% of PStV and other BCMV subgroup. These results provide evidence that PStV and other BCMV subgroup are in the same virus with different strain.
Pelembagaan Konsep Pengendalian Hama Terpadu di Indonesia
Kasumbogo Untung
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada
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DOI: 10.22146/jpti.12392
Indonesia for a period of ten years (1989-1999) has trained more than one million farmers through a national programme of IPM Farmers' Field School especially on riceand other food crops. The success of farmers training based on participatory approach in Indonesia has been recognized globally as one of the pioneer of the Ecological Integrated Pest Management paradigm. The follow-up activities after the National IPM Programme which need to be done is to institutionalize IPM concept among government, industries and community institutions. Due to the presence of conflicts between ecological IPM paradigm and technological IPM paradigm which formally and legally supported by the government and the industry, processes of institutionalization of IPM in Indonesia will require a large efforts and funds, and take a long time.
Pemanfaatan Enzyme-Linked Immunosorbent Assay untuk Mengukur Titer Virus dalam Bawang Putih
Susamto Somowiyarjo;
Endang Mugiastuti;
Y. M. Sugi Maryudani
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada
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DOI: 10.22146/jpti.12407
Non-precoated Indirect ELISA had been developed by employing monoclonal antibodies against virus isolated from Sangga variety of garlic. The ELISA was used to measure the titer of virus in the plant. In comparation with biological assay using Chenopodium amaranticolor, ELISA was able to measure the virus titer faster and was more simpler. The highest titer of virus was obtained using the first leaf of garlic at age of 29-36 days after planting. Application of nitrogen at high dose and high temperature of garlic cultivation trends to increase the virus titer. The results of this experiment may be used to improve the method of sampling to detect virus in garlic tissues.
Injuries on Seedlings Caused by Potential Weed in Tropical Rain Forest Regeneration Areas
Sumardi Sumardi;
S. M. Widyastuti
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada
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DOI: 10.22146/jpti.12393
The experiment aimed to assess the injuries on Shorea seedlings caused by weed in artificial regeneration of tropical rain forest in Jambi. Four planting systems, strip nurse planting (using Acacia mangium, Paraserianthes falcataria and Gmelina arborea), line planting, gap planting and natural regeneration, were used. Seedling injuries were assessed based on part of seedling suppressed. Results indicated that Shorea seedling suffered from varying degrees of injuries, depending on weed species and part of the seedling suppressed. The dominance of weed and damage intensity were determined by the level of canopy opening on the planting systems. Ground cover dominated rapidly in open canopy, causing up to 55.27% injuries on the seedlings in the strips of G. arborea and P. falcataria. Whereas creepers and vines became dominant in moderate canopy opening. The injury of Shorea seedling planted under nurse tree was determined by the species and planting density of nurse tree used. Light canopy nurse tree such as P. falcataria failed to suppress ground weed, but in the contrary, A. mangium with heavy canopy still allowed creepers and vines to grow.
Evaluasi Potensi Parasitoid Penggerek Pucuk Tebu, di Kabupaten Bantul
Eddy Mahrub;
Sri Ambarwati Amini;
Nugrahaning Rahayu
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada
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DOI: 10.22146/jpti.12403
The objective of the research is to evaluate the potency of sugar cane top borer parasitoids whether the parasitoids are available from the released individuals produced previously in the laboratory or other species naturally exist in the field. The result showed that Trichogramma sp. which have been released did not attack sugar cane top borer eggs. Tetrastichus schoenobii and Telenomus rowani observed in the field were seemingly replacing the role of Trichogramma sp. parasitoid previously released in the area. Both the T. schoenobii and T. rowani attacked the sugar cane top borer eggs with parasitism of 34.59% and 24.07% respectively. Larval parasitoids were Elasmus zehntneri, Rhaconotus scirpophagae, Stenobracon nicevillei and Allorhogas sp. while pupal parasitoid was Isotima javensis. Larval parasitism was 0-41.00% averagely. The most dominant parasitoid was R. scirpophagae with parasitism of 18.75% followed by E. zehntneri with parasitism of 17.25% respectively. Total parasitism by all species were 37.59% in young plant and 23.90% in old plant respectively. Pupal parasitism on young and old plants were 13.66% and 8.26% respectively. Those parasitoids were available in the field naturally and performed important potentiality in the natural control of sugar cane top borer. Those above mentioned parasitoids need to be conserved by preventing the ecosystem suffering from any hazardous changes, which may afflict negative effects to the natural enemies.