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All Journal Buletin Hama dan Penyakit Tumbuhan Jurnal Perlindungan Tanaman Indonesia JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA Jurnal Penelitian Pertanian Terapan Jurnal Agrotek Tropika Jurnal Agrotropika Jurnal Pengabdian Fakultas Pertanian Universitas Lampung Jurnal Proteksi Agrikultura

Hasriadi Mat Akin

Jurusan Proteksi Tanaman, Fakultas Pertanian Universitas Lampung, Bandar Lampung

Author-ID : 278269

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Earth & Planetary Sciences Environmental Science

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Pola SegregasiKarakter KetahananTanaman Kedelai(Glycine max [L]. Merrill)Terhadap Infeksi Soybean Mosaic Virus Populasi F2 Keturunan Taichung X Tanggamus Wanda, Nidya; Barmawi, Maimun; Akin, Hasriadi Mat; Sa?diyah, Nyimas
Jurnal Penelitian Pertanian Terapan Vol 15, No 1 (2015)
Publisher : Politeknik Negeri Lampung.

This study aims to determine the pattern of segregation character soybean plant resistance to infection SMV and other agronomic characters as well as many genes that play a role in the inheritance of these traits in the F2 populations from crosses Taichung x Tanggamus follow of Mendel ratio or a modification of expectations and knowing the number of crosses. This research was conducted at the Laboratory LapanganTerpadu Faculty of Agriculture, University of Lampung from September 2013 until January 2014, and then continued in the laboratory observations Seed and Plant Breeding, University of Lampung. Propagation of virus carried in Kampung Baru, Bandar Lampung. This research uses experimental design without repetition with a single treatment design nested structured data is analyzed using the chi-square test for the suitability of the normal distribution and chi-squared test to test the of Mendel ratio and modifications . The results of this study indicate the character frequency distribution of plant height, grain weight per plant soybeans F2 generation from crosses Taichung x Tanggamus normal spread, while the frequency distribution for character flowering age, harvesting, healthy seed number per plant, number of productive branches, number of pods per plants and severity of disease spread is not normal. Estimates of the number of genes that control the character of harvesting is controlled by two genes are recessive epistasis duplicate the ratio 9: 7, days to flowering follow the ratio 1: 2: 1 which is controlled by a single gene that reacts not perfect, healthy character of the number of seeds per plant followed the ratio 3: 1 and for the total character of pods per plant, number of productive branches as well as the severity of the disease follows the ratio of 13: 3. the character dikendalikanolehdua genes dominant-recessive epistasis react. There are 21 numbers belonging expectations resistant to SMV. Keywords: Soybean, segregation, resistance characteristics, and SMV.

UJI KETAHANAN TERHADAP COWPEA MILD MOTTLE VIRUS PADA SEMBILAN BELAS POPULASI F1 TANAMAN KEDELAI (GLYCINE MAX [L.] MERRIL) HASIL PERSILANGAN DIALEL Barmawi, Maimun; Utomo, Setyo Dwi; Akin, Hasriadi Mat; Ramli, Sulastri
JURNAL AGROTROPIKA Vol 14, No 2 (2009): Agrotropika Vol.14 No.2 2009
Publisher : JURNAL AGROTROPIKA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (147.696 KB)

The objective of this study was to evaluate the resistance to cowpea mild motlle virus (CPMMV) of 19 F1 population of soybean showing good agronomic characters. The F1 population were generated by complete diallel crosses involving a resistant parent (Mlg 2521), a moderately resistant parent (B 3570), and three susceptible parents (Orba, Wilis, and GKUL-1). The experiment was arranged in a completely randomized block design consisted of two replications. The results indicated that F1 population B 3570 x Mlg 2521 was resistant to CPMMV and superior in seed weight per plant, number of branches, plant height, total number of pods, total number of seeds, and the number of healthy seeds. F1 population Wilis x B 3570 and B 3570 x Wilis were moderately resistant and less superior in agronomic characters than B 3570 x Mlg 2521.Key words: CPMMV, diallel, Glycine max,viral resistance

Biological and molecular variability among PStV (peanut stripe virus) strains isolated from various peanut growing areas in Indonesia was studied based on their pathogenicity and coat protein genes. Six strains of PStV causing different types of symptoms on peanut leaves were used to study biological and molecular variability among these strains. Biological variability of the PStV strains was evaluated based on their pathogenicity on peanut cultivars, Gajah and Landak. While analysis of the coat Hasriadi Mat Akin; Sudarsono Sudarsono; Rusmilah Suseno
Buletin Hama dan Penyakit Tumbuhan Vol. 11 No. 2 (1999): Buletin Hama dan Penyakit Tanaman
Publisher : Buletin Hama dan Penyakit Tumbuhan

Show Abstract | Download Original | Original Source | Check in Google Scholar

Biological and molecular variability among PStV (peanut stripe virus) strains isolated from various peanut growing areas in Indonesia was studied based on their pathogenicity and coat protein genes. Six strains of PStV causing different types of symptoms on peanut leaves were used to study biological and molecular variability among these strains. Biological variability of the PStV strains was evaluated based on their pathogenicity on peanut cultivars, Gajah and Landak. While analysis of the coat protein gene was used to study the molecular variability of PStV strains. Based on their pathogenicity, six PStV strains could be grouped into three groups, medium, strong, and very strong strains. Analysis of a coat protein gene showed the variability of the PStV strains were 0-2.1percent based on nucleotide sequence and 0-1.4percent based on predicted amino acid sequence. There was no correlation between biological and molecular variability.

Variabilitas Strain-Strain PStV pada Tingkat Molekul Berdasarkan Gen Protein Selubung dan 3' UTR (Untranslated Region) Genom RNA PStV Hasriadi Mat Akin; Sudarsono Sudarsono
Jurnal Perlindungan Tanaman Indonesia Vol 8, No 2 (2002)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12318

Variability of various peanut stripe virus (PStV) strains at the molecular level was studied based on nucleotide sequence of coat protein (CP) cistron and 3' UTR (untranslated region). Six PStV strains causing different types of symptoms on peanut leaves (Arachis hypogaea var. Landak) were used in this study. Fourteen point mutations on the coat protein gene have been identified among the PStV strains, however, only two point mutations at three positions (9309 of PStV-lsb and PStV-lbs and 9885 of PStV-Ibs) resulted in amino acid residue changed of the coat protein. Pair comparison among six 3' UTR sequences of the strains revealed a 97.9 - 100% sequence similarity. The results provided evidence that all the strains were of the same virus and there is no correlation between typical symptoms of the PStV infection and CP sequence variability among the strains.

Analisis Runutan Nukleotida Gen Protein Selubung Dua Strain Peanut Stripe Virus Hasriadi Mat Akin
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 1 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12406

Nucleotide sequence analysis of coat protein gene from two biological strains of peanut stripe virus (PStV), severe blotch (PStV-Isb) and stripe (PStV-Is), has been done to study molecular variation of these strains. Comparison of predicted amino acid sequence of PStV coat protein with other members of bean common mosaic virus (BCMV) subgroup was also carried out to study the taxonomic: relationship between PStV and BCMV subgroup. The CP gene from these strains contained 861 nucleotides (nts) and encoded 287 amino acids. There were seven nucleotides of the CP gene and one nucleotide of NIb cistron that were different between PStV-Is and PStV-Isb. However, only one nucleotide at position 9309 of the CP gene caused differential amino acid residue at position 49 of the coat protein, methionine (M) for PStV-Isb and isoleucine (I) for PStV-Is. Comparison of the ammo acid sequences revealed a sequence similarity of 89,9-90,9% of PStV and other BCMV subgroup. These results provide evidence that PStV and other BCMV subgroup are in the same virus with different strain.

HERITABILITAS, NISBAH POTENSI, DAN HETEROSIS KETAHANAN KEDELAI (Glycine max [L.] Merrill) TERHADAP SOYBEAN MOSAIC VIRUS Nyimas Saâ€™diyah; Hasriadi Mat Akin; Ria Putri; Risa Jamil; Maimun Barmawi
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 16 No. 1 (2016): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Heritability, potential ratio, and heterosis of soybean (Glycine max [L.] Merrill) resistance to soybean mosaic virus. The use of soybean cultivars with resistance to SMV is a way for controlling soybean mosaic disease. The objective of this research was to estimate the disease severity, the narrow sense heritability, potential ratio and heterosis of resistance character and number of pithy pods, number of healthy seeds, and healthy seeds weight per plant of ten F1 populations of soybean crossing result to SMV infection. The experiment was arranged in a randomized complete block design in two replications. Observed characters were disease severity, number of pithy pods, number of healthy seeds, and healthy seeds weight per plant. The result of this research showed that 1) the crossing combinations those which were resistant to SMV (lower disease severity) were Yellow Bean x Tanggamus, Tanggamus x Orba, and Tanggamus x Taichung, 2) the narrow sense heritability of disease severity was included in medium criteria, 3) number of pithy pods belonged to high criteria, and 4) number of healthy seeds and healthy seeds weight per plant were included in low criteria. The crossing combinations that had low estimationÂ value of heterosis and heterobeltiosis of resistance to SMV infection were Yellow Bean x Taichung, Bean x Tanggamus and Tanggamus x B3570. Disease severity or resistance to SMV is influenced by genetic and environmental factors.

EFEKTIVITAS SATELIT RNA YANG BERASOSIASI DENGAN CUCUMBER MOSAIC VIRUS (CARNA-5) UNTUK MENGENDALIKAN PENYAKIT VIRUS PADA TANAMAN TOMAT Hasriadi Mat Akin; Muhammad Nurdin; Patrisius Binsar Simamora; Marlaba Sitorus
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 12 No. 2 (2012): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Two strains of CMV associated satellite RNA (CARNA 5.1 and CARNA 5.2) were evaluated to control severe strain of Cucumber mosaic virus (CMV-G), Tobacco mosaic virus (TMV), and Potato virus Y (PVY) on tomato plants. Tomato plants were mechanically inoculated with CARNA 5.1 and CARNA 5.2 to protect the plants from super infection of CMV-G, TMV, and PVY. The result revealed that inoculation of CARNA 5.1 and CARNA 5.2 to tomato plants effectively controlled the plants from CMV-G super infection. The effectiveness of CARNA 5.1 and CARNA 5.2 to unrelated virus, TMV and PVY, was evaluated in this experiment. The result showed that CARNA 5.1 and CARNA 5.2 inhibited TMV and PVY infection. CMV-G super infection on protected tomato plants showed mild disease symptoms, significant redution of plant growth, height, and yield; however, nonprotected plants showed severe disease symptoms and significant inhibition of plant growth and tomato yield.

KETAHANAN TERHADAP PENYAKIT BERCAK DAUN LAMBAT (CERCOSPORIDIUM PERSONATUM) DAN KARAKTER AGRONOMI KACANG TANAH FAMILI F5 KETURUNAN PERSILANGAN KELINCI x SOUTHERN RUNNER Setyo Dwi Utomo, Ety Setiowati, dan Hasriadi Mat Akin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 5 No. 2 (2005): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

The resistance to late leaf spot (Cercosporidium personatum) and agronomic characters of peanut F5 families derived from crosses between Kelinci x Southern Runner. Late leafspot disease in peanut can be controlled by planting resistant cultivars. The objective of this study was to evaluate the resistance of nine F5 families derived from crosses between Kelinci and Southern Runner. Gajah, Kelinci, Panter, and Southern Runner were used as controls. Experiments was arranged in a randomized complete block design with three replications. The resistance was evaluated based on the score of disease severity. The results indicated that F5 families of K/SR-2 and K/SR-9 were more resistant to late leafspot and yielded higher than Gajah, Panter, and Kelinci. Families K/SR-7 and K/SR-8 were tolerant to late leafspot.

KEPATOGENAN SATELIT RNA YANG BERASOSIASI DENGAN CUCUMBER MOSAIC VIRUS (CMV-satRNA) PADA TANAMAN CABAI Hasriadi Mat Akin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 5 No. 1 (2005): MARET, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

Pathogenicity of RNA satellite associated with cucumber mosaic virus (CMV-satRNA) on hot pepper plant. The objective of this experiment was to determine the pathogenicity of RNA satellite associated with CMV (CMV-satRNA) on hot pepper plant and effects of mix infection of CMV-satRNA and other viruses naturally infecting hot pepper, PVY (potato virus Y) and TMV (tobacco mosaic virus). Two green house experiments were conducted in Gedong Meneng Bandar Lampung during June—November 2004. The treatments of the the first experiment were arranged in a completely randomized design with five replications. The treatments were hot pepper plant inoculated with CMV-satRNA, severe strain CMV-G, and control uninoculated plant. The second treatment was arranged in a complete block randomized design in a factorial experiment with four replications. The first factor was single inoculation of CMV-satRNA, PVY, TMV, double inoculatoin of CMV-satRNA and PVY, CMV-satRNA and TMV, PVY and TMV, and control uninoculated plant. The second factor was two hot varieties, Taro and Laris. The results of the first experiment showed that infection of CMV-satRNA did not significantly reduce the yield of hot pepper plant compared to severe strain CMV-G. The result of the second experiment indicated that mix infection of CMV-satRNA and TMV or CMV-satRNA and PVY did not induced a synergetic reaction on hot pepper plants.

KETAHANAN TIGA SPESIES ARACHIS TERHADAP BERCAK DAUN AKHIR (CERCOSPORIDIUM PERSONATUM BERK. ET CURT.) PADA PEMBERIAN DAN TANPA PEMBERIAN MANKOZEB Setyo Dwi Utomo dan Hasriadi Mat Akin .
Jurnal Hama dan Penyakit Tumbuhan Tropika Vol. 4 No. 2 (2004): SEPTEMBER, JURNAL HAMA DAN PENYAKIT TUMBUHAN TROPIKA
Publisher : Universitas Lampung

The resistance of three species of Arachis to late leaf spot (Cercosporidium personatum) with and without application of mankozeb. The use of resistant cultivars and application of fungicide are effective means to control late leafspot. Resistant genotypes of peanut is required as a parent for hibridization to develop high-yielding cultivars resistant to late leaf spot. The objective of this study were to evaluate the resistance of 11 genotypes from three species of Arachis to late leaf spot (C. personatum) with and without application of mankozeb. The experiment was arangged ia a split splot design with three replications. The main plots were fungicide treatment (treated and untreated). Fungicide mankozeb ( 4 g/l, 500 l/ha) was applied at 28, 40, 52, 64 and 76 days after planting. The sub-plots were genotypes of species Arachis, i.e., 7 lines of cultivated peanut (Archis hypogaea) from ICRISAT, 2 national varieties (Gajah and Kelinci), and two wild species (A. cardenasii, and A. pintoi). The two wild species were imune to late leaf spot so that they can be utilized as resistant parent in wide hybridization to develop a superior cultivars. ICGV 88262 was more resistant than Gajah and Kelinci. Because its yield tend to be lower than Gajah and Kelinci, ICGV 88262 also can be utilized as resistant parent in hibridization. Mankozeb was efective and substantially reduced yield losses of cv. Gajah. Mankozeb should be aplied in cultivation of cv. Gajah to control late leafspot.

Co-Authors Agnes Ratnasari Anggraini, Desma Bambang Irawan /E21207042 Cipta Ginting Cipta Ginting, Cipta Denny Marini Sihite Dwi Endarwati Dwi Yara Zutta Olivia Efri Efri Efri Efri Ermawati Ermawati Galih Prasetyo Geraldo Sandy Hamim Sudarsono . Helina, Selvi Herry Susanto Heru Pranata I Gede Swibawa Ichwan Surya Nugraha Ivayani Ivayani Ivayani Ivayani Ivayani Ivayani Jefry Fernando Purba Joko Prasetyo Joko Prasetyo Joko Prasetyo Lestari Wibowo Mahfut Maimun Barmawi Maimun Barmawi Maimun Barmawi Marlaba Sitorus Maya Gusmarini Muhamad Idola Hartas Muhammad Nurdin Muhammad Nurdin Muhammad Nurdin Muhammad Nurdin Muhammad Nurdin Muhammad Syamsoel Hadi Nidya Wanda Nidya Wanda Nyimas Saâ€™diyah Nyimas Saâ€™diyah Patrisius Binsar Simamora Pramesti, Elisa Amelia Radix Suharjo Radix Suharjo Ramli, Sulastri Reny Destya Angrum Ria Putri Risa Jamil Riski Mardiana Rusdi Evizal RUSMILAH SUSENO Sa?diyah, Nyimas Saefudin Saefudin Safitri, Annisa Sari, Mai Sembiring, Rinawati Setyo Dwi Utomo Solikhin Solikhin Sudarsono Sudarsono Sudarsono Sudi Pramono Sudi Pramono Suskandini Ratih Dirmawati Titiek Nur Aeny Titik Nur Aeny Tivanny Sherrin Wanda Tri Maryono Yusticia, Stenia Ruski Yuyun Fitriana

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