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Y. Andi Trisyono
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INDONESIA
Jurnal Perlindungan Tanaman Indonesia
Published by Universitas Gadjah Mada
ISSN : 14101637     EISSN : 25484788     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 6, No 2 (2000)" : 7 Documents clear
Response of A European Corn Borer, Ostrinia nubilalis, Population to Selection with An Ecdysone Agonist, Tebufenozide Y. Andi Trisyono
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (8578.46 KB) | DOI: 10.22146/jpti.12389

Abstract

Response of a population of the European corn borer, Ostrinia nubilalis (Hubner), to selection with an ecdysone agonist, tebufenozide (RH-5992), was investigated. The selection was carried out by exposing newly hatched larvae of O. nubilalis on an artificial diet containing 0.285 ppm tebufenozide for four days. Throughout seven generations of selection, a slight decrease in the larval mortality, an increase in the pupal mortality, and a decrease in the number of offspring produced per female were noticed. After four generations of selection, a shift in susceptibility of the selected O. nubilalis larvae to tebufenozide was not observed. The acute and chronic effects of tebufenozide caused the loss of O. nubilalis population after seven generations of selection.
Memahami Prinsip Ambang Penyakit Serangga dalam Konteks Epizootiologi F. X. Susilo
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12368

Abstract

Disease phenomena in insect population can be understood through epizootic modeling. In a pathosystem, disease is generated by transmission (ρ) and is decayed by death (μ). Mass incidence and decay of disease depend on the disease threshold density (St*) which inherently possesses three traits. First, if the density of susceptible insects (St) > St* then disease incidence (It) will increase ( ΔIt > 0 ). Second, if St < St*, then It will decrease ( ΔIt < 0 ). Third, if St = St* then there will be no new infection ( ΔIt = 0 ). Threshold density can be determined monocyclically through laboratory bioassays and polycyclically through field observation. The values of μ and ρ, which are the principal components of monocyclic threshold density, may indicate whether the corresponding entomopathogen is the better candidate for microbial insecticide or, otherwise, even better as the biological control agent (natural enemy). Meanwhile, the concept of polycyclic threshold density highlights the importance of incorporation of sampling of infected insects into the existing agro-ecosystem monitoring scheme.
Tanggapan Helicoverpa armigera terhadap Infeksi Subletal Nuclear Polyhedrosis Virus dan Dampaknya terhadap Laju Reproduksi Gita Pawana; Tjandra Anggraeni; Anna Laksanawati H.D.
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12387

Abstract

The study on responses of Helicoverpa armigera Hbn. to sublethal HaNPV (H. armigera nuclear polyhedrosis virus) infection was carried out in the laboratory. The reproduction rate was scrutinized as their impact of HaNPVs exposures. Fifth instar of H. armigera larval was orally infected on sublethal serial dilution of HaNPV (1,800; 180; 18; 1.8; 0.18; 0.018 ppm from stock concentration of 1.1 X 10^9 PIB per ml suspension). A significantly female pupal weight forms the response of H. armigera to sublethal HaNPV infection on the concentration tested. However larval period, male pupal weight, pupal period, oviposition period, life span of imago, fecundity, fertility and survival did not significantly form the response of H. armigera to sublethal HaNPV infection. All treatment showed lower reproduction rate compared to the untreated, especially reproduction rate based on either debilitating effect and mortality or just based on debilitating effect.
Paraquat Herbicide in Peat Soil: I. Its Effects on the Dynamics of Microbial Population Sebastian Margino; Erni Martani; Bambang Hendro Sunarminto
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12386

Abstract

Paraquat has been used widely and periodically in peat soil. It is stable in acid environments, therefore its application in peat soil which represents an acid environment, might prolong its persistence. Liming treatment has known to reduce peat soil acidity. This research was conducted to study the effect of paraquat and liming treatments on the dynamics of microbial population in peat soil. Unlimed and limed peat soil were treated with paraquat to a final concentration of 20 ppm, and incubated for 2 months. Microbiological analysis, consisting of counting of bacterial, actinomycetes, and fungal population were done weekly. The changes of pH value and paraquat residue were also measured. The results showed that in unlimed peat soil, paraquat treatment did not influence microbial population. However, when paraquat was added into limed peat soil, the number of microbial population decreased; especially the population of bacteria. Liming treatment increased bacterial population and changed the population dynamics of actinomycetes. No significant difference of fungal population in peat soil treated with paraquat and lime. Additionally, there was no significant difference in paraquat resistance between limed and unlimed peat soil.
Tanggapan Planlet Vanili yang Diradiasi dengan Sinar Gamma dan Keragaannya Setelah Diinokulasi dengan Jamur Fusarium oxysporum f.sp. vanillae Woerjono Mangoendidjojo; Bambang Hadisutrisno; Alfi Inayati
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12390

Abstract

Vanilla is one of spice crops and important to world commerce. Indonesia is one of producing and exporting countries for decades. Recently, the planted area tends to decrease due to several factors. One of them is fusarium wilt caused by Fusarium oxysporum fsp. vanillae. The disease ranked among the most devastating disease attacking vanilla plants. The most effective method controlling fusarium wilt is the use of resistant varieties. The study aimed to know responses of vanilla plantlets irradiated by Gamma rays at several doses (0-4 krad). Five months after irradiation, the survival mutant plantlets were inoculated with suspension of the F. oxysporum fsp. vanillae to evaluate their resistance. Data of first concern taken were survival plantlets; number of leave, root, and sucker initiation; and infection intensity. The results indicated that higher the doses, decreased the survival plantlets. The applied doses performed significantly differences on the number of leave, root, and suckerinitiation. The untreated plantlets showed better responses as compared to those treated. Evaluation on their resistance after inoculation indicated that the survival plantlets irradiated at 3 and 1 krad showed lower infection intensity, eventhough variation within a treatment was observed. Evaluation on the individual plantlet basis seemed to be more helpful in order to identify mutant plantlets with better resistance.
Pemanfaatan Antibodi Monoklonal dalam Immunoassay untuk Deteksi Baculovirus oryctes Susamto Somowiyarjo; Hery Haryanto; Sri Wening
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12384

Abstract

The application of non-precoated Indirect Enzyme-Linked Immunosorbent Assay (ELISA) and Dot Immunobinding Assay (DIBA) employing monoclonal antibodies (MCA) against Yogyakarta isolate of Baculovirus oryctes Huger. was described. The MCA-Bv-4 having subclass of IgG2a and titer in vitro of 10^4 - 10^5 proved to be useful antibody for virus detection. The great potential of I-ELISA using MCA-Bv-4 has been it's specificity being able to discriminate between healthy an virus-infected coconut beetle (Oryctes rhinoceros L.). The assay could also differentiate between B. oryctes and Monodon baculovirus, the pathogen of shrimp disease. The best tissue for preparing virus antigen from crude extract was found to be the midgut of the beetle. Head, thorax, abdomen and tibia were not suitable for preparing the test antigen. The crude extract of beetle showed high endogenous enzyme activity to the substrate of DIBA, which precluded the detection of B. oryctes using DIBA. The MCA-Bv-4 could be used to improve the monitoring of the virus to support the program of biological control of coconut beetle using B. oryctes.
Symptoms Induction by Pseudorecombination of RNA Species Between CMV Strains W. S. Wahyuni; Y. Sulyo; I. B. Raharjo; E. B. Trisusilowati
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12370

Abstract

Twenty seven strains of CMV have been collected throughout Java, they have four RNA species and none of them contain satellite RNA. The exchange of RNA species of strains belonging to subgroup I and II for pseudorecombination induced mild to severe symptoms on Nicotiana glutinosa. By aphid transmission, the CMV pseudorecombinant could produce a typical symptom of one of its parental strain or expressed unlikely symptoms as those of either parental strains on N. glutinosa. Some plants with mild symptoms were detected to be positive by Indirect ELISA.

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