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Efektivitas Isolat-Isolat Bacillus sebagai Pengendali Penyakit Bulai dan Pemacu Pertumbuhan Tanaman Jagung pada Kondisi Terkontrol Khoiri, Syaiful; Badami, Kaswan; Pawana, Gita; Utami, Ciwuk Sri
Rekayasa Vol 14, No 2: Agustus 2021
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v14i2.10270

Abstract

The main disease in maize is downy mildew caused by the fungus Peronosclerospora spp.. This pathogen can cause yield losses of up to 100%. Therefore, efforts to control this disease are continuously carried out, including technical culture, assembly of resistant plants, and use of synthetic fungicides. At the farm level, the use of metalaxyl, synthetic fungicides is the most common practice. On the other hand, it has been reported that some Peronosclerospora groups are starting to become resistant to metalaxyl. These problems lead to the need for alternative controls, for example with biological agents. Biological agents from bacterial groups have been developed to control plant disease, but for downy mildew is still limited. The purpose of this study is to screen and test Bacillus spp. ability to suppress downy mildew and promote the growth of maize. The assay was carried out on seeds by invitro to investigate growth-promoting reactions and also testing under controlled conditions in greenhouses to investigate the suppression ability of downy mildew disease development. The results showed B. polymyxa strain BP18, Bacillus subtilis strain BS41, Bacillus sp. strain BT1, and Bacillus sp. strains can stimulate the growth of corn seedlings and suppress downy mildew. The best isolate in suppressing downy mildew was Bacillus sp. strain BT1 with the smallest AUDPC value (3.94) and the highest protection index (82.71%). It is hoped that these results will find potential isolates and have the potential to be developed into biopesticides and biofertilizers.
VALIDASI BAKTERI PELARUT FOSFAT INDIGENUS MADURA PADA RHIZOSFER Gita Pawana
Agrovigor Vol 7, No 2 (2014): September
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.974 KB) | DOI: 10.21107/agrovigor.v7i2.1444

Abstract

ABSTRACTOften generalized that the determination effectiveness of biological agents depends on environment. The purpose of this study is to obtain an explanation of the role of growth limiting factors, chemical or physical on the effectiveness Madura indigenous Pseudomonas aeruginosa as a phosphate solubilizing. The method used in achieving the goal of research is, perform in vitro studies on the type of carbon source (sugar) and the level of salinity as the growth limiting factors of bacteria on the effectiveness of phosphate dissolution and study the influence of the the rice rhizosphere type of phosphate available. Found that in vitro, the type of carbon source and salinity levels affect phosphate concentrations and abundance of bacterial cells. Increased levels of salinity decreases the abundance of bacterial cells. Average cell population fluorescent pseudomonads in fields rice rhizosphere is higher than the lowland rice rhizosphere, the cell population fluorescent pseudomonads higher level provide higher available phosphate and vegetative growth of rice better.Key words:  Phosphate solubilizing, limiting growth, carbon source, salinity, the rhizosphere Madura indigenous.
Studi Distribusi Aktif dan Penularan Nematoda Entomopathogen Heterorhabditis spp Isolat Lokal dibawah Tanah Gita Pawana; Achmad Amzeri; Ahmad Djunaidy
Agrovigor Vol 10, No 2 (2017): September
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v10i2.4316

Abstract

Sehubungan dengan terbatasnya informasi dasar dari aktivitas nematoda entomopatogen (NEP) isolat lokal (Bangkalan Madura) sebagai entomopathogen bagi serangga tanah yang sebenernya merupakan inang dan habitat aslinya, selanjutnya tujuan dari penelitian ini adalah untuk mengetahui dan menganalisa distribusi aktif Heterorhabditis spp isolat lokal pada kondisi tanah dengan struktur lepas terkait dengan keberadaan bahan organik dan mikrob, serta potensi penularannya. Pemdekatan metode yang digunakan adalah dengan meletakkan inang NEP pada berbagai kedalaman  dan jarak horizontal, serta menambahkan berbagai kandungan bahan organik dan mikrob pada media terdistribusi dan analisa mono siklus penyakit dan populasi inang. Selanjutnya mortalitas inang dianalisa 4 hari setelah suspensi NEP diberikan pada media. Hasil dari penelitian menunjukkan bahwa distribusi vertikal keatas atau kebawah juga horizontal dari Heterorhabditis spp isolat lokal dalam waktu 4 hari optimum sampai pada kedalaman 24 – 26 cm dari permukaan tanah. Kandungan bahan organik dan mikrob pendekomposer tanah tidak mempengaruhi kemampuan distribusi. Kedua isolat juga dapat bertindak sebagai sumber inokulum untuk penularan dalam populasi inangnya dengan jumlah invidu lebih dari 7 – 8  ekor, dengan periode letalnya 13 hari.
Kompatibilitas dan Efektifitas Fungi Mikorisa Arbuskula (FMA) terhadap Kacang Komak (Dolichos lablab L) Sidqi Zaed Z.M.; Gita Pawana; Slamet Supriyadi
Agrovigor Vol 1, No 1 (2008): SEPTEMBER
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (74.2 KB) | DOI: 10.21107/agrovigor.v1i1.231

Abstract

Besides, low nutrient availability  the problem in  dry land of Madura  is  the conflict  of land use  for food crops and  forage  production. Overcoming the problem  a program that  unite agriculture and husbandry activities is needed.  The objective of this research was to find out the most compatible and effective isolate of AMF on hyachinth bean (Dolichos lablab L)  as a forage legume.  The AMF which were   Gigaspora sp, Glomus sp, Glomus manihotis, Acaulospora sp dan Entrophosopra sp  were  planted beneath the bean seeds in polybag and   the plants were managed in a green house in optimal condition. The result showed that among isolates of arbuscular micorrhizal fungi, Gigaspora sp. greatly influenced growth and yield of biomass of hyachinth bean (Dolichos lablab L) indicating dependency of the plant to the fungus.  It is concluded that Gigaspora sp is the most compatible isolate for legume of hyachinth bean (Dolichos lablab L).
INTERAKSI PSEUDOMONAD PENDARFLUOR INDIGENUS DENGAN Glomus aggregatum TERHADAP SERANGAN PENYAKIT BATANG BERLUBANG DAN PERTUMBUHAN TANAMAN TEMBAKAU Gita Pawana; - Syekhfani; Tini Surtiningsih; Wiwiek Sri Wahyuni
Agrovigor Vol 5, No 2 (2012): SEPTEMBER
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v5i2.312

Abstract

Properties are favorable for the growth of plants from mycorrhizal arbuscular fungi and pseudomonads fluorescenct still need the wider examine,  the positive interaction that may be obtained from their association. The purpose of this study was to examine the interaction of Pseudomonas fluorescens Pfim20 with Glomus aggregatum in the rhizosphere of tobacco and evaluate whether the interaction can increase of growth and suppress hollow stalk disease. The method of research is evaluation of antagonistic P. fluorescens Pfim20 towards Pectobacterium carotovorum, followed by green house experiment. The results obtained,  the association of P. fluorescens Pfim20 with G. aggregatum positive interaction, on the presence or the absence of association between of P. fluorescens Pfim20 with G. aggregatum there are not level of disease severity and progression of hollow stalk disease, positive interaction between P. fluorescens Pfim20 with G. aggregatum can increase the uptake of phosphate, but can not give a higher biomass.
Pertumbuhan dan Produksi Tanaman Kacang Hijau (Vigna radiata L.) Pada Berbagai Konsentrasi Osmolit Sorbitol dan Intensitas Cekaman Kekeringan Suhartono Suhartono; Gita Pawana; Sulistri Sulistri
Agrovigor Vol 13, No 2 (2020): September
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrovigor.v13i2.8414

Abstract

Kemampuan tanaman untuk tumbuh normal pada kondisi tercekam kekeringan disebut sebagai daya adaptasi. Salah satu teknologi budidaya yang dapat diterapkan untuk meningkatkan daya adaptasinya pada kondisi tercekam kekeringan adalah dengan penyemprotan osmolit pada daun tanaman. Tujuan dari penelitian ini adalah untuk mengetahui respon pertumbuhan dan produksi tanaman kacang hijau akibat pemberian osmolit sorbitol pada intensitas cekaman kekeringan yang berbeda. Penelitian dilakukan di dalam rumah kaca dengan menggunakan RAL faktorial yang terdiri dari 2 faktor, yaitu konsentrasi sorbitol dengan 4 taraf (0,10,20, dan 30 ml/l) dan intensitas cekaman kekeringan dengan 4 taraf (tidak tercekam, tercekam kekeringan mulai stadia vegetatif, pembungaan, dan pengisian polong sampai panen). Hasil penelitian menunjukkan pemberian osmolit sorbitol 10, 20, dan 30 ml/l belum mampu meningkatkan produksi kacang hijau pada kondisi tercekam kekeringan seperti tanaman yang dibudidayakan pada kondisi normal (tidak tercekam kekeringan), namun untuk mendapatkan hasil aplikasi osmolit sorbitol yang efesien yaitu dengan menggunakan konsentrasi 30 ml/l yang dilakukan pada tanaman yang mendapat cekaman kekeringan paling lama yaitu mulai stadia vegetatif.
Respon Bibit Tebu Akibat Komposisi Media Tanam yang Diaplikasikan Formulasi Dobel Inokulan Pseudomonad Pendarflor dan Azospirillum Gita Pawana; Achmad Djunaedy; Syaiful Khoiri
Agrovigor Vol 12, No 1 (2019): Maret
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (284.139 KB) | DOI: 10.21107/agrovigor.v12i1.5287

Abstract

Untuk mendapatkan bibit berkualitas baik toleran terhadap cekaman biotik dan abiotik dengan perakaran baik dan efisien terhadap pemupukan pada media tanam diaplikasikan formulasi dobel inokulan pseudomonad pendarflor dan Azospirillum, namun demikian pada pembibitan tebu informasi tentang hal ini masih terbatas. Tujuan penelitian ini adalah untuk mengetahui pengaruh komposisi media tanam yang diaplikasikan formulasi dobel inokulan pseudomonad pendarflor dan Azospirillum terhadap pertumbuhan bibit budchip tebu. Budchip ditumbuhkan pada nampan pembibitan dengan komposisi media tanam sebagai perlakuan yaitu M1: top soil dan bahan organik dengan komposisi 1:1, M2: top soil dan formulasi dobel inokulan pseudomonad pendarflor dan Azospirillum dengan komposisi 1:1, M3: top soil dan formulasi dobel inokulan pseudomonad pendarflor dan Azospirillum dengan komposisi 2:1, M4: top soil dan formulasi dobel inokulan pseudomonad pendarflor dan Azospirillum dengan komposisi 1:2. Digunakan rancangan acak lengkap. Pada parameter data dilakukan analisa varian, jika terdapat perbedaan yang siknifikan dilanjutkan dengan uji jarak berganda Duncan dengan taraf nyata (α) 5%. Parameter data yang diamati meliputi: tinggi tanaman, diameter batang, jumlah daun, bibit yang hidup, jumlah akar primer dan sekunder, dan biomassa tanaman. Kesimpulan yang diperoleh adalah  formulasi konsursium pseudomonad pendarfluor dan Azospirilum dapat meningkatkan pertumbuhan pembibitan tebu, dapat menstimulasi pertumbuhan akar sekunder.  Adapun media tanam dengan komposisi top soil dan formulasi dobel inokulan pseudomonad pendarflor dan Azospirillum 1:1 merupakan komposisi yang paling ideal.
INOKULASI Azospirillium sp DARI LAHAN KERING MADURA TERHADAP PERTUMBUHAN TANAMAN JAGUNG Ach. Khoirul Ikhwan; Ahmad Waqik; Mohammad Anwar; Ummu Fitrothul; Dwi Rahmawati; Gita Pawana
Agrovigor 2015: Vol 8, No 2 (2015) SEPTEMBER
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (314.069 KB) | DOI: 10.21107/agrovigor.v0i0.985

Abstract

Penelitian ini bertujuan untuk memperoleh isolat bakteri Azospirillium sp dari Madura, serta mengkaji kehandalannya dalam menggantikan pupuk N sintetik. Samel tanah diambil dari Kecamatan Galis, Geger dan Tanahmerah, isolasi dilakukan dengan menggunakan media semi solid Nfb, selanjutnya diuji kehandalannya berdasarkan pengaruhnya terhadap pertumbuhan tanaman jagung. Diperoleh 1 isolat yang terbaik yaitu isolat yang diisolasi dari rhizosper tanaman Graminae di Kecamatan Galis, namun demikian berdasarkan pertumbuhan tanaman jagung belum bisa menggantikan pupun N sintetik.Kata kunci : Azospirillum sp, jagung, nitrogen, isolat madura
Tanggapan Helicoverpa armigera terhadap Infeksi Subletal Nuclear Polyhedrosis Virus dan Dampaknya terhadap Laju Reproduksi Gita Pawana; Tjandra Anggraeni; Anna Laksanawati H.D.
Jurnal Perlindungan Tanaman Indonesia Vol 6, No 2 (2000)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jpti.12387

Abstract

The study on responses of Helicoverpa armigera Hbn. to sublethal HaNPV (H. armigera nuclear polyhedrosis virus) infection was carried out in the laboratory. The reproduction rate was scrutinized as their impact of HaNPVs exposures. Fifth instar of H. armigera larval was orally infected on sublethal serial dilution of HaNPV (1,800; 180; 18; 1.8; 0.18; 0.018 ppm from stock concentration of 1.1 X 10^9 PIB per ml suspension). A significantly female pupal weight forms the response of H. armigera to sublethal HaNPV infection on the concentration tested. However larval period, male pupal weight, pupal period, oviposition period, life span of imago, fecundity, fertility and survival did not significantly form the response of H. armigera to sublethal HaNPV infection. All treatment showed lower reproduction rate compared to the untreated, especially reproduction rate based on either debilitating effect and mortality or just based on debilitating effect.
Karakter Kuantitatif Melon Generasi S0-S4 Siti Rohmatin; Kaswan Badami; Gita Pawana
Rekayasa Vol 15, No 2: Agustus 2022
Publisher : Universitas Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/rekayasa.v15i2.15109

Abstract

One of the obstacles to melon cultivation is the availability of seeds and the high price of melon seeds. This condition causes the need to assemble superior melon varieties with the expected characters. This study aims to form a pure line of melon with certain superior characteristics and can be proposed as a new high-yielding variety in the open-pollinated category. The research was conducted at the Experimental Garden of the Agrotechnology Study Program, Faculty of Agriculture, Trunojoyo University, Madura, from September-December 2022. This study used a randomized block design method. The treatments used were nine pure lines of melon (PK-669, D-162, PK-361, PK-165, PK-114, D-029, D-5089, PK-211, and comparison). Each treatment was repeated three times, and each experimental unit consisted of 12 plants. Research data were analyzed using analysis of variance (ANOVA) and HSD test α = 0.05. The selection advancement was analyzed by comparing character data for each generation. The research showed that the results of the characterization of ten melon lines S0-S4 generation on the characters of fruit length, fruit diameter, flesh thickness, and melon fruit weight showed a decreasing trend in each generation. The S4 generation (9.17 °brix) in the character of the average sugar content there was an increase that was not significantly different from the S3 generation (9.03 °brix), while the number of seeds in each generation had an average value that was not significantly different but occurred an increase in the number of seeds in the S3 (372) and S4 (381) generations. An increase in one generation of the population in the linear regression equation is estimated to reduce the character of fruit length by 0.50 cm, fruit diameter by 0.465 cm, flesh thickness by 0.140 cm, fruit weight by 0.199 Kg, sugar content by 0.752 °brix, and a number of seeds by 5.4.