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INDONESIAN JOURNAL OF PHARMACY

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Published by Universitas Gadjah Mada

ISSN : 23389427 EISSN : 23389486 DOI : -

Core Subject : Health,

Medicine & Pharmacology

Indonesian Journal of Pharmacy (ISSN-e: 2338-9486, ISSN-p: 2338-9427), formerly Majalah Farmasi Indonesia (ISSN: 0126-1037). The journal had been established in 1972, and online publication was begun in 2008. Since 2012, the journal has been published in English by Faculty of Pharmacy Universitas Gadjah Mada (UGM) Yogyakarta Indonesia in collaboration with IAI (Ikatan Apoteker Indonesia or Indonesian Pharmacist Association) and only receives manuscripts in English. Indonesian Journal of Pharmacy is Accredited by Directorate General of Higher Education (DGHE) DIKTI No. 58/DIKTI/Kep/2013.

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Articles 8 Documents

Search results for , issue "Vol 26 No 2, 2015" : 8 Documents clear

ANTIPROLIFERATIVE ACTIVITY OF RECOMBINANT HUMAN INTERFERON ALPHA2B ON ESTROGEN POSITIVE HUMAN BREAST CANCER MCF-7 CELL LINE Ratih Asmana Ningrum; Popi Hadi Wisnuwardhani; Adi Santoso; Neng Herawati
Indonesian Journal of Pharmacy Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Indonesia is the top three countries with hepatitis and moreover has 40.000 cancer mortalities per year. Interferon alpha 2b (IFNα-2b) is a therapeutic standard for cancer and hepatitis B/C treatments. We developed recombinant human interferon alpha 2b (rhIFNα-2b) in methilotropic yeast Pichia pastoris X-33. The protein was produced as extracellular protein with 24 kDa in size. This research was aimed to characterize the protein based on its amino acid sequence and to study its antiproliferative activity on MCF-7 cell line. Amino acid sequencing by using MALDI TOF TOF mass spectrometry with trypsin as proteolytic enzyme identified protein as hIFNα-2b with 33% of amino acid coverage. The antiproliferative activity was determined by 3-[4.5-dimethyltiazol-2il]-2.5-diphenylltetrazolium bromide (MTT) assay. Based on several studies about the synergistic activity of rhIFNα-2b with other anticancer drugs, we combined our rhIFNα-2b with tamoxifen (tmx). The growth percentage of the cells after being treated with 1µM of tmx at various concentrations of rhIFNα-2b was compared with that of untreated cell. This study showed that the antiproliferative activity was dose-dependently. Cell viability assay with calcein and ethidium bromide-based staining by fluorescence microscope confirmed that the rhIFNα-2b had ability to inhibit proliferation of human breast cancer cell line MCF-7. Keywords: human interferon alpha 2b, Pichia pastoris, antiproliferative and MCF-7

OPTIMIZATION OF POLYSACCHARIDE-RICH FRACTIONATION FROM MORINDA CITRIFOLIA L. FRUIT BASED ON IMMUNOSTIMULATORY EFFECT IN VITRO Sasmito, Ediati; Hertiani, Triana; Kartika, Senda; Putri, Faradhyta Maharani; Setiawan, Vania; Narastika, Longina
Indonesian Journal of Pharmacy Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Morinda citrifolia L. fruits are rich in polysaccharides of which are non toxic and possess hospes-mediated-antitumor potential. In Indonesia, noni fruit has been used empirically to enhance immune system. Previous research revealed that noni polysaccharide-rich fraction showed macrophage activity enhance-ment. This research aimed to optimize the method of noni-polysaccharides-rich fractionation using four different methods for polysaccharide isolation (method I, II, III, IV) and to evaluate the immunostimulatory effects of the isolated polysaccharides on macrophages and lymphocytes activities. Immunostimulatory effects were examined on male Balb/c mice in vitro. The polysaccharides were evaluated by phenol sulfuric acid test. Qualitative methods were used to detect the phytochemical characteristic. Total phenolic content was determined by using Folin-Ciocalteau method. The study showed different methods resulted in different yields, phytochemical characteristics and immunostimulatory activities of polysaccharides-rich fraction. The phytochemical test from method I, III and IVb revealed the presence of alkaloid. Method II resulted fractions with the presence of saponin. There were no protein and phenolic substances detected from all methods. The highest result of polysaccharide content was found in method IVb (32.58%). Method III showed the highest activity on phagocytic activity of the macrophage, while method IVb exhibited the highest stimulation on lymphocyte proliferation.Key words: Isolation method, polysaccharide, Morinda citrifolia L. immunostimulatory effect

FAST DISSOLVING TABLETS OF PIMOZIDE: DESIGN, OPTIMIZATION AND INVITRO CHARACTERIZATION Raja Rajeswari Kamisetti; Moses Mekala; Sudhakar Muvvala; Durga Bhavani Penmatsa
Indonesian Journal of Pharmacy Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

As precision of dosing and patient compliance become an important prerequisite for a long-term treatment of Tourette’s syndrome there is a need to develop formulation for this drug, which overcomes problems such as difficulty in swallowing, inconvenience in administration while travelling and patient’s acceptability. The present work was undertaken with a view to develop a fast dissolving tablets of Pimozide using Kyron T-314 as super-disintegrant along with Avicel PH 102 as diluent by response surface method using direct compression. Drug-excipient compatibility studies were confirmed by FTIR Spectroscopy. The tablets were evaluated for hardness, friability, weight variation, wetting time, disintegration time and uniformity of content and invitro dissolution. Based on evaluating parameters, formulation prepared by using 4.5% Kyron T-314 with 11.5% Avicel PH-102 was selected as optimized formulation and Formulation (F3) had disintegration time of 7.63±0.25s. and percentage cumulative drug release of 81.60 after 10min. The formulations were further studied and confirmed for their stability. Hence it was concluded that direct compression using Kyron T-314 superdisintegrant and Avicel PH 102 was simple and economic technique which can be used for formulation of fast dissolving tablets of Pimozide.Key word: Pimozide, Tourette’s syndrome, fast dissolving tablets, Kyron T 314, direct compression

PREPERATION AND CHARACTERIZATION OF β-CYCLODEXTRIN INCLUSION COMPLEXES ORAL TABLETS CONTAINING POORLY WATER SOLUBLE GLIMIPIRIDE USING FREEZE DRYING METHOD Yandi Syukri; Laryssa Fernenda; Fissy Rizki Utami; Isna Qiftayati; Aris Perdana Kusuma; Rochmy Istikaharah
Indonesian Journal of Pharmacy Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Glimepiride is an oral antidiabetic drugs which is practically insoluble in water. The formation of β-cyclodextrin inclusion complex was able to increase the solubility of glimepiride. This study aim to prepare, characterize and formulation of inclusion complex tablets in order to meet the requirement in Pharmacopeia. The inclusion complex were prepared in a molar ratio of 1:1 and 1:2 by freeze drying method, afterthat characterized include FTIR spectroscopy and scanning electro microscope (SEM). Further, it was formulated into tablets by direct compression technique using primogel and crospovidone as superdisintegrants. The tablets were evaluated include weight uniformity, hardness, friability, disintegration, and dissolution. The dissolution studies of inclusion complex were performed by using USP II apparatus. The result of FTIR and SEM provided evidence of the formation of complexes after utilizing freeze-drying methods. The tablet evaluation containing inclusion complex glimepiride-β cyclodextrin with primogel and cropovidone as disintegrant showed that increased concentration of disintegrant will increase disintegration time of the tablets. All of formulas meet the requirements in the Pharmacopoeia. The inclusion complex of glimepiride–β cyclodextrin successfully used for enhancing the solubility of glimepiride and the tablets meet the requirement in Pharmacopeia.Keywords: Glimepirid, β-cyclodextrin, primogel, crospovidone

PROTECTIVE EFFECT ETHANOLIC EXTRACT OF Boesenbergia pandurata (ROXB.) Schlecht. AGAINST UVB-INDUCED DNA DAMAGES IN BALB/C MICE Shanti Listyawati; Sismindari Sismindari; Sofia Mubarika; Yosi Bayu Murti
Indonesian Journal of Pharmacy Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Boesenbergia pandurata (Roxb.) Schlecht. contains bioactive compounds that have a number healthy effect including anti-oxidant and anti-carcinogenic activity. This research was carried out to examine the protective effect of B. pandurata extract against expression of cyclobutane pyrimidine dimers (CPDs) as marker of UVB-induced DNA damage in Balb/c mice. Dried powder of B. pandurata rhizomes was extracted by maceration method using 96% ethanol. The extract was quantified with pinostrobin as active marker using TLC scanner. Ethanolic extract of B. pandurata (EEBP) was given orally at 14 days before UV exposure with a variety doses, 0 (vehicle), 20, 40 and 60mg/kgBW/day and continuing until termination of the experiment. Following the UVB irradiation (1.4J/m2), mice were sacrificed at different time points (2, 24, 48, and 72h after UVB exposure). The back skin samples were collected to analyze CPDs expression by immunohistochemical method. The result showed that EEBP (contained 5% pinostrobin) dose was 40 and 60mg/kgBW/day had protective activity against UV-induced DNA damage as indicated by the decrease of CPDs expression. Key words: Boesenbergia pandurata (Roxb.) Schlecht., UVB, DNA damage, CPDs.

SOLUBLE EXPRESSION OF SYNTHETIC CSF3syn GENE FUSED WITH THIOREDOXIN IN Escherichia coli BL21(DE3) THROUGH AUTOINDUCTION METHOD AND PURIFICATION Riyona Desvy Pratiwi; Asrul Muhamad Fuad
Indonesian Journal of Pharmacy Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

A synthetic human gene of CSF3 (CSF3syn.Ec3), coding for hG-CSF was succesfully subcloned into pET32a(+) expression vector and fused with thioredoxin (Trx) at its N-terminal as fusion partner. The obtained fusion gene of Trx-CSF3syn within the recombinant plasmid pET32a(+)_CSF3yn.Ec3 was verified by PCR, plasmid restriction, and DNA sequencing analysis. In order to investigate the fusion gene expression, we transformed Escherichia coli BL21(DE3) as the host with the recombinant plasmid. The gene was succesfully expressed within the cytosol as fusion protein of Trx·tag, His·tag, S·tag, EK-site, and hG-CSF moities. By the autoinduction method, it was obtained 49% from the soluble fraction and 51% from the insoluble fraction. The soluble fraction was subsequently purified by IMAC method (Ni-NTA) and characterized.Key words : hG-CSF, thioredoxin, autoinduction, IMAC, E.coli.

SYNTHESIS OF TETRAHYDROHEXAGAMAVUNON-5 AND TETRAHYDROHEXAGAMAVUNON-7 Ritmaleni Ritmaleni; Ian Praditya; Haryono Wibowo; Sardjiman Sardjiman
Indonesian Journal of Pharmacy Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Synthesis of Tetrahydrohexagamavunon-5 (THHGV-5) and Tetrahydrohexagamavunone-7 (THHGV-7) were prepared by catalytic hydrogenation reaction on Hexagamvunon-5 (HGV-5) and Hexagamavunone-7 (HGV-7) by using gas H2 as source of hydrogen gas, Pd/C 10 % as metal catalyst and methanol as solvent at room temperature. The products were characterized by IR Spectroscopy, Gas Chromatography-Mass Spectroscopy (GCMS), 1D-NMR (1H-NMR and 13C-NMR) and 2D-NMR (1H-13C HMQC) Spectroscopy to determine the product structure molecules. According to the data of IR, GC-MS, 1H-NMR, 13C-NMR and 1H-13C HMQC spectra, the products are THHGV-5 and THHGV-7 as white crystalline powders. Key words:Tetrahydrohexagamavunon-5, Tetrahydrohexagamavunone-7, Hexagamvunon-5, Hexagamavunone-7

IN VITRO RELEASE MODELING OF ASPIRIN FLOATING TABLETS USING DDSOLVER Agus Siswanto; Achmad Fudholi; Akhmad Kharis Nugroho; Sudibyo Martono
Indonesian Journal of Pharmacy Vol 26 No 2, 2015
Publisher : Faculty of Pharmacy Universitas Gadjah Mada, Yogyakarta, Skip Utara, 55281, Indonesia

Aspirin has low solubility in water therefore, dissolution is a rate limiting step for absorption. Floating tablet formulation is designed to improve the bioavailability of aspirin. The objective of this study was to determine in vitro dissolution study of aspirin floating tablet release kinetics model. The floating tablets were prepared by a direct compression method using Methocel K4M CR, NaHCO3, Ethocel, Aerosil, and dicalcium phospate anhydrous as excipients. Tablets were evaluated by different parameters such as physicochemical properties, floating lag time (Flag time), total floating time, and dissolution. The result showed that the tablet mass has good flow properties of 13.54 g/sec. Aspirin floating tablets had a weight uniformity (CV=1.45%), good hardness (6.42kg), and low friability (0.158%). The tablet has a short Flag time of 25.16 sec and long floating time of 8 hours. Dissolution data were evaluated using DDSolver conducted by (1) Statistical parameters: R2adjusted, AIC, MSC; (2) Visual goodness of fit (GOF). The results showed that aspirin floating tablets release kinetics followed the Korsmeyer-Peppas model. Aspirin release occurs through the mechanism of anomalous transport which combines Fickian diffusion and polymer relaxation.Key words: aspirin floating tablet, DDSolver, modeling of drug release

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