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INDONESIA
International Journal of Biosciences and Biotechnology
Published by Universitas Udayana
ISSN : 23033371     EISSN : 26559994     DOI : -
Core Subject : Science, Social,
Biochemistry, Genetics & Molecular Biology Environmental Science
International Journal of Biosciences and Biotechnology provides a unique venue for publishing original researches in biosciences and biotechnology, and ensures that authors could reach the widest possible audience. It publishes both full-length articles and short communications on all aspects of biotechnology and biosciences
Arjuna Subject : -
Articles 7 Documents
 1 
Search results for , issue "Vol 3 No 2 (2016)" : 7 Documents clear
ELECTRIC STUNNING OF CATTLE FOR SLAUGHTERING AND SECURING THE BEEF FROM MICROORGANISM Wayan Sayang Yupardi; I Made Nuryasa; Ni Luh Putu Sriyani; Eny Puspani; I Gede Suranjaya
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Stunning electricity current of 1.0 A, 200 – 300 V were caused the destruction of cattle’s centralnerve. This is very important to drain blood as much as possible from the whole body. Thiscauses its beef not become a media of unexpected microorganism to develop theirself.In themodern age right now, electricity is needed much for living on the earth or outer space. In otherwords, live is much depend on it. Indonesia is a tropical and agricultural country with highhumidity. These are good media for microorganism to develop theirself i.e. in aerobic conditionsuch as Pseudomonas, Achromobacter (induce rancidity in), Strepcococcus, Leuconostoc,Bacillus (causes mucus on beef surface), Lactobaccillus (causes the changes of beef colour fromred to greenish), Photobacterium (causes phosphorescent). anaerobic codition Achromobacterand Proteus cause sour taste. The beef or other food stuff become spoiled fast if they are notpreserved in low temperatureIt is necessary to control the room temperature. The objectives ofthis study were to know and open insight about physiological works of electricity in order toprovide healthy beef as a source of animal protein to form a bright nation. The beef can bepreserved more than 30 days without decreasing its hygiene level in the temperature of 5 – 7oC.At these low temperatures the microorganism mentioned above can delayed their growth anddevelopment.
Guide for Authors Guide for Authors
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Guide for Authors
DISTRIBUTION OF CVPDr GENE AMONG SOME CITRUS PLANTS IN BALI I Gede Putu Wirawan
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Citrus Vein Phloem Degeneration (CVPD) is the main disease of citrus plant caused by a Gram negativebacteria, Candidatus Liberobacter asiaticus. The disease is also called citrus greening disease or recentlycalled citrus huanglongbing. The CVPDr gene was firstly found in Triphacia trifoliata a citrus relativeplant which considered to be resistant to CVPD disease. The distribution of the gene among the citrusrelative plants in Bali were studied. The result of this study so far, CVPDr gene were found in theseedless lime (Citrus aurantiifolia) and Triphacia trifoliata which are known as a resistant or tolerant toCVPD disease, however, Citrus maxima, Citrus nobilis, Citrus reticulate, and Citrus amblycarpa are allsensitive plants but their harboring the CVPDr gene. This result indicated that CVPDr gene was not givea resistant to the disease in these plants. This results suggested that other/s gene is needed to give aresistant or tolerant in these plants.
APPLICATION OF NATURAL COLOR MIXED AND NaCl AS MORDANT ON WOOD FIBER USING SIMULTANEOUS MORDANTING Ni Wayan Bogoriani
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Wood is one of the few materials that is highly exploited by humans, resulting in increasing usage of low quality wood. High demand and usage on high-quality wood causing heavy reduction of its availability. To increase artistic value of low quality wood, staining on the surface of the wood need to be done. This research aims to apply a mix of natural pigments (Gambier- Piper betle leaves- Areca seeds) and NaCl salt as the mordant on the surface of Albesia wood fiber and test the physical and chemical dye properties with 1% detergent. This study uses a mixture of natural dyes (Gambier- Piper betle leaves- Areca nut) with various concentrations and salt as auxiliaries, carried out with the simultaneous method of mordanting. This study was divided into 7 groups. Differences in mean between groups were tested using One Way ANOVA followed by Post Hoc LSD, which revealed statistically significant test when p value < >0.05. The results showed that there were significant differences of natural dyes mixed applications (Gambier- Piper betle leaves- Areca seeds) in the ratio of 5.0: 3.0: 3.0 to 0.5 g of salt as auxiliaries and 10 minutes of immersion with the simultaneous method of mordanting. Ratio of the dye mixture, on the surface of the wood causes brown color and has the highest absorption average of 0.19 gram and the durability of the dye showed the highest seen from the appearance of color and mix of the missing mass. It can be concluded that the best application is with a mixture of natural dyes and the addition of 0.5 g of NaCl salt on the surface of Albisia wood fiber with the simultaneous method of mordanting and also based on the durability of the dye test is a ratio of 5.0: 3.0: 3.0.
Content Articles Content Articles
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Content Articles
EFFECT OF RESTING TIME ON PERIPHERAL BLOOD MONONUCLEAR CELL YIELDS Inna Narayani; Rasmaya Niruri; Nyoman Mantik Astawa
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Cryopreservation of PBMC (peripheral blood mononuclear cells) was done to preserve and analyze the number of PBMC derived from blood samples which come in at different time. The batch analysis wasperformed at the same time in order to reduce variations in results. The analysis on the cells numbers carried out after 1, 3, 6, 12, and 24 hours. Heparinized whole blood was collected from healthy subject by venipuncture, and stored at room temperature. Blood is processed by centrifugation in Ficoll density gradient following the established method of Balai Besar Veteriner Denpasar. Buffy coat layer was collected and washed twice with HBSS (Hank's balanced salt solution) and was counted in Turk’s solution. The cells were then dissolved in 1 ml of cold freezing medium containing 10% DMSO and 50% FBS (fetal bovine serum) and stored overnight at -80°C before storage in liquid nitrogen vessel for few weeks. The samples rapidly thawed in a water bath at 37°C and washed twice with PBS (phosphate buffered saline). The cells were stored in 4°C PBS and counted in Turk’s solution after 1, 3, 6, 12, and 24 hours. The results obtained were varied with a declining trend.
POTENTIAL UTILIZATION IN DYE DECOLORIZATION OF SPENT MUSHROOM SUBSTRATES Hee-Wan Kang
International Journal of Biosciences and Biotechnology Vol 3 No 2 (2016)
Publisher : Central Laboratory for Genetic Resource and Molecular Biology, Faculty of Agriculture, Udayana University in cooperation with Asia-Oceania Bioscience and Biotechnology Consortium (AOBBC)

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Abstract

Pleurotus ostreatus, P. eryngii, and Flammulina velutipes are main edible mushrooms that account forover 89% of total mushroom production in Korea. Of them, cultivation of P. eryngii is 25%. About 2.5million tons of spent mushroom substrate (SMS) is produced each year in Korea. It is either used asgarden fertilizer or deposited in landfills, which pollutes the environment. This study was carried out toextract the lignocellulolytic enzymes amylase (EC 3.2.1.1), cellulase (EC 3.2.1.4), laccase (EC 1.10.3.2),and xylanase (EC 3.2.1.8) were efficiently extracted from spent mushroom substrate (SMS) of ediblemushrooms. laccase activity were highest in extracts from the P. eryngii SMS, with values of 8.01 U/g.The synthetic dyes remazol brilliant blue R and Congo red were decolorized over 88 and 93% by thewater extract of P. eryngii SMS within 120 min, and the extract’s decolorization ability was compared tocommercial laccase. Furthermore, the SMS extract was applied to decolorize textile mill wastewater dyefor its industrial application.

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