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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2 Documents
 1 
Search results for , issue " Vol 14, No 1 (2015)" : 2 Documents clear
RAGAM GENETIK TIGA POPULASI SEPAT SIAM (Trichopodus pectoralis Regan; Osphronemidae) ASAL KALIMANTAN MENGGUNAKAN ANALISIS RAPD DAN PENGUKURAN MORPHOMETRIC TRUSS [Genetic Diversity of Three Populations of Snakeskin Gourami (Trichopodus pectoralis Regan; Osphronemidae) from Kalimantan Based on RAPD Analysis and Truss Morphometrics Measurements] Iskandariah, Iskandariah; Soelistyowati, Dinar Tri; Gustiano, Rudhy; Kusmini, Irin Iriana; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (557.892 KB) | DOI: 10.14203/beritabiologi.v14i1.1866

Abstract

The aim of this study was to evaluate the genetic diversity of three populations of Snakeskin Gourami (Trichopodus pectoralis;Osphronemidae)from three provinces of Indonesian Borneo:West, Central, and South Kalimantan using Random Amplified Polymorphism DNA (RAPD) and morphometric truss methods.DNA amplification using primer OPC-02,OPC-05,and OPA-09 resulted in 21 to 28 fragments with sizes ranged from 200 to 1600 bp, polymorphism value was of 7.14 to 25.00%, heterozygosity was of 0.02 to 0.11 and the genetic distance between populations was of 0.27 to 0.28.Truss morphometrics analysis showed that the coefficient of variability was ranging between 2.75 to 12.52%.There were 9 characters that can be used as diagnostic characters for Snakeskin Gourami. The intra population similarity index in Snakeskin Gourami populations from West Kalimantan was the highest (80%) followed by populations from Central Kalimantan (16.7%) and South Kalimantan (3.3%).The results of RAPD and truss morphometric analysis suggested that populations from West Kalimantan have higher genetic diversity than populations from Central and South Kalimantan.
ISOLASI DAN AKTIVITAS PLANT GROWTH PROMOTING RHIZOBACTERIA (RHIZOBIUM, AZOSPIRILLUM, AZOTOBACTER, PSEUDOMONAS) DARI TANAH PERKEBUNAN KARET, LAMPUNG [Isolation And Activity Of Plant Growth Promoting Rhizobacteria (Rhizobium, Azospirillum, Azotobacter, Pseudomonas) From Soil Of Rubber Plantation, Lampung] Widawati, Sri
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (840.976 KB) | DOI: 10.14203/beritabiologi.v14i1.1868

Abstract

Analysis of phosphate dissolving activity, P dissolved; PMEase and IAA production by Plant Growth Promoting Rhizobacteria group(PGPR)is a parameter to determine the effectiveness of these bacteria as biological organic fertilizer (BOF).This study was aimed to obtain PGPR (Rhizobium, Azospirillum, Azotobacter, Pseudomonas) that were potential as a BOF to reclamation on degraded plantation area. This study used a random sampling method for 11 sampling points (1999P1, 1999P2, 1999P3, 1999P4, 1999P5, 2007P, 2007P1,2007P2, 2007P3, 2007P4, 2007P5) in the area of rubber plant roots.Isolation and counting of bacterial populations used plate count method on selective media (YEMA, Okon, Caceres, NFB AMA, PAB) whereas phosphate dissolving activity analysis, Dissolution Efficiency Index (DE), PMEase and IAA production following the method of Nguyen, Bray, Seshadri, Tabatabai, and Gravel.The results showed that of the 11 sampling points, 11 isolates were obtained effectively consisting of four groups of bacteria, namely: Rhizobium,5 5 5 5 Azospirillum, Azotobacter and Pseudomonas with the number of population: 3.3x10 , 80x10 , 20x10 , and 14x10 cfu.The results of the analysis of DE, soluble P, PMEase acid-base, and the highest IAA obtained from the bacterium isolated from the sampling point 1999P3 and 1999P1. This potential isolates will be used to the next research on reclamation of degraded land plantation

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