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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : - EISSN : - DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 14 Documents

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Search results for , issue " Vol 9, No 3 (2008)" : 14 Documents clear

INDUKSI KALUS DAN ORGANOGENESIS KULTUR IN VITRO Dendrobium lineale Rolfe Hoesen, Djadja Siti Hazar; Witjaksono, Witjaksono; Sukamto, LA
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.790

Callus induction and organogenesis were evaluated in the protocorm like bodies (PLB) culture of Dendrobium lineale Rolfe (Orchidaceae). The globular PLB were cultured in Murashige and Skoog (MS) medium with half strength macronutrients QA MS), supplemented with thidiazuron (TDZ 0,1, 0,5 and 1) mg/1 and 2,4 Dichlorophenoxy acetic acid (2,4-D 0.1, 0.5, 1 and 5) mg/1 as treatments. The media containing 1 mg/1 TDZ and (1 and 5) mg/1 2,4-D was the best treatment for callus initiation (100%). The largest diameter of callus was obtained from 1 mg/1 TDZ (average 3.5 cm). Shoot buds regeneration achieved on 1 mg/1 TDZ (average 41.66 %). However roots regeneration was very low (average 6.66 %) obtained from 1 mg/1 2,4-D. The number of adventitious buds produced from the regenerated shoots on media without plant growth regulator

DETEKSI LOGAM BERAT PADA PERAIRAN, SEDIMEN DAN SIRIP IKAN BADUKANG {Anus caelatus HAN A. maculatus) DIMUARA SUNGAI KAHAYAN DAN SUNGAI KATINGAN, KALIMANTAN TENGAH Sanusi, Harpasis S; Harteman, Edison; Soedharma, Dedi; Winarto, Adi
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.783

The study sites were located in the river mouths of Kahayan and Katingan Rivers of Central Kalimantan. The purpose of this study was to evaluate concentration of heavy metals on the water, sediment, Arius (catfish) fin through concentration and bioconcentration factor (BCF); data were analysed by regression and correlation analysis. Collected samples were water and fish.Water sample and fish bone were analysed by Atomic Absorption Spectrophotometer (AAS). The results indicated that the concentrations of Pb>Cd>Hg were occurred in the water and hard fins, which the bioconcentrations of Hg>Cd>Pb were in the hard fins, while the bioconcentration factors (BCFs) of Pb>Hg>Cd were in the sediment.Moreover, it seems that heavy metals in the waters absorpted and accumulated by sediment and hard fins increased along with heavy metal concentration in the water, whereas heavy metals in the sediment absorpted and accumulated by hard fins increased along with heavy metal concentration .in the sediment.

AKTIVITAS NITROGENASE BINTIL AKAR DAN KADAR KLOROFIL DAUN KACANG HIJAU {Phaseolus radiatus L.) PADA BERBAGAI JENIS DAN KADAR ASAM FENOLAT Rauf, AWahid
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.787

Phenolic acids are one of the many secondary metabolites causing toxic upon the growth and development of plant. Phenolic acids are produced by plant and subsequently released into soil, and appears to be involved in biochemical interaction between plants and other living organisms in soil. The research was conducted to study the effect of various kinds and doses of phenolic acids on nitrogenases activities and cholorophyll content of mungbean. Research was done in the glass house of Soil Science Departemen, Faculty of Agriculture, Gadjah Mada University (GMU) from April to June 2004. The experiment was arranged by randomized block design in three replications. The treatment consist of four kinds phenolic acid i.e. vanilic, p-coumaric, ferulic and p-1hydroxybenzoic with concentration at 250, 500, 1000 and 1500 mg kg-Ã respectively. One treatment was without phenolic acids as control. Result indicated that phenolic acids could reduce nitrogenases activities and chlorophyll content up to 53.35% and 29.98% respectively. The toxicity of p-hydroxybenzoic against both nitrogenases activities and chlorophyll content was higher than other phenolic acids.

RESVERATROL 10C-GLUKOPIRANOSIDA DAN HEIMIOLA, KARAKTER Hopea gregaria SECARAKEMOTAKSONOMI Sahidin, Sahidin; Hakim, EH; Syah, YM; Juliawaty, LD; Achmad, SA; Din, LB; Latip, J
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Ten resveratrol oligomers have been isolated from the stem bark of H. gregaria i.e. resveratrol lOC-glucopyranoside (1), heimiol A (2), balanocarpol (3), ampelopsin A (4), hopeaphuran (5), E-viniferin (6), parviflorol (7), a-viniferin (8), hopeaphenol (9), and vaticanoi B (10). The structures of these compounds were determined base on spectroscopic methods including UV, IR, 1-D NMR, 2-D NMR and comparison with the reported data. Implication of the compounds invention is not only strengthen conclusion that Hopea tends to produce resveratrol dimmers but also showed that resveratrol lOC-glucopyranoside (1) and heimiol A (2) are phytochemical markers of. H. gregaria.

ANALISIS SENYAWA BIO AKTIF DARI EKSTRAK BUI PICUNG (Pangium edule Reinw.) SEGAR Mangunwardoyo, Wibowo; Ismaini, Lily; Heruwati, Endang Sri
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pangi (Pangium edule Reinw.) seed has long been used traditionally as a preservative agent for fish, especially in remote areas.A study has been conducted on analysis of bioactive compounds of pangi fresh seeds extracts and their fractions.In this study,maceration of fresh seed using water and 50% ethanol was carried out followed by thin layer chromatography (TLC) analysis to see weather the extracts contained tannin. Both extract then separated into their respective fractions using column chromatography.Fractions which had been tested to have highest antibacterial activity were then analysed using gas chromatography mass spectrometry (GC-MS) to assess the active compounds which believe to be a preservative agent.Identification of water and 50% ethanol extract of Pangium edule Reinw.fresh seeds with TLC resulted that tannin were found in those extracts. Furthermore, GC-MS analysis showed that fractions which had been previously tested to have high antibacterial activity contained 9-octadecanoic acid whith similarity index of 89%, and 1,2-benzenedicarboxylic acid whith similarity index of 94-85%.

AKTIVITAS ENZIM PROTEASE DARI Mucorjavanicus YANG DITUMBUHKAN PADA MEDIA TEPUNG SINGKONG {Mannihot utilissima) Choliq, Abdul
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The experiment was conducted to study the activity of protease from Mucor javanicus in cassava flour media. The influence of peptone and incubation time to the production of the enzyme,and the influence of pH and temperature to the activity of protease were studied. The production of protease was conducted in the flask (100 ml) which contain 17.5 g cassava flour and SO ml aqueous media.The species of M.javanicus observed consist of 10 strains. The strain of M.javanicus which has the highest production of protease among the tenth was incubated in cassava flour media with 3 variations of incubation time (4, 7 and 10 days) and 5 variations of peptone concentration (1-5%). The pH and temperature used for protease characterization were pH 4.0-7.0 and 30-70Â°C, respectively. The results show that the highest protease production with the optimal incubation period 7 days (0.51 U/ml)was M. javanicus M3. The optimal concentration of peptone for protease production was 2 %. The optimal protease activity was reached in pH 5 and temperature 50ÃÂ°C with the value of 4.53 U/ml.

TINGKAT KELANGSUNGAN HIDUP FRAGMEN KARANG Acroporaformosa YANG DITRANSPLANTASIKAN PADA MEDIA BUATAN YANG TERBUAT DARI PECAHAN KARANG (RUBBLE) Fadli, Nur
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Coral reefs around the world continue to decline.Corals killed by natural or anthropogenic disturbances are often degraded into rubble. This rubble is dynamic, easily shifted by storms and currents which may forms "killing fields" for coral juveniles, hindering coral recovery. In order to rehabilitate the coral reef, artificial substrates are always used as artificial reefs both for coral transplantation and recruitment. Unfortunately, most artificial substrates are expensive and used material from outside of the ocean (for example concrete/cement base). In order to develop a new low-cost artificial substrate that can be replacing the concrete/cement-base as a media for coral transplantation and coral recruitment, the modified coral rubble were tested in Seribu Islands, Jakarta. Two different net (nylon and polyethylene) were used to form or to shape rubble into a compact shape, stable and strong substrate.The stability of the rubble and the complexity of the surface which is created by the net make this substrate suitable for coral transplantation and coral recruitment. In 6 m, the highest survival was recorded for coral fragments that were transplanted on cement-base (58%). The lowest survival was recorded on nylon + rubble (16%). While, in 10 m, the highest survival was found on cement-base (40%) and the lowest was on polyethylene + rubble (3%). The modified coral rubble is a potential method for coral transplantation and coral recruitment. However, this approach requires testing at additional sites to determine the replicability of the results.

STUDI FITOKIMIA Ocimum spp.: KOMPONEN KIMIA MINYAK ATSIRI KEMANGI DAN RUKU-RUKU Sulianti, Sri Budi
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Isolation of essential oils of two species of Ocimum (kemangi and ruku-ruku) were done by hydrodistilation. The chemical composition of those essential oils were analysed by GC-MS. Totaly 38 chemical components of kemangi essential oil were identified, and six of them are the major chemical constituents, ie. ethyl hexadecanoic acid (17.72 %), ethyl octadecanoic acid (14.83 %), ethyl 9-octadecenoic acid (10.62 %), methyl eugenol (4.88 %), Z-citral (7.02 %) and geranial (7.86 %), On the other hand, the essential oil of ruku-ruku contain 31 components with the main five major chemical components, i.e. ethyl hexadecanoic acid (19.33 %), ethyl octadecanoic acid (15.39 %), ethyl 9-octadecenoic acid (11.30 %), methyl eugenol (8.69%) and zerumbona (4.76 %).

ANALISIS BEBAN PENCEMARAN DAN KAPASITAS ASIMILASI DANAU SENTANI, PAPUA SEBAGAIUPAYAKONSERVASI LINGKUNGAN PERAIRAN Walukow, Auldry F; Djokosetiyanto, D; KholiPdan, KholiPdan; Soedharma, Dedi
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.777

The Lake Sentani has problems. Firstly, high of erosion number i.e. 94.52 ton/ha/year of which the value is higher than erosion tolerance at average 25 ton/ha/year.Secondly, high concentration of chemical pollution such as Cu (0.0201- 0.1081 mg/L) and Zn (0.21 - 0.36 mg/L) which these values are above water quality standard approved by the government as ruled in PP 82 Tahun 2001 about the management of water quality and water pollution. Therefore research and management are needed to find solution for the sustainability of this lake. The aims of this research is to be acquainted with carrying capacity of Lake Sentani through analyzing 1) the pollution load, and 2) the assimilation capacity of Lake Sentani aquaculture. The collected information is important for future aquaculture environmental management and conservation. Result shows that the pollution loads from river are obtained as follows (ton/month): TDS (441.806 to 775.287), BOD (3.510 to 7.801) and COD (7.737 to 16.055).The assimilation capacities from lake are obtained as follows (ton/month): TDS (12.18494), BOD (11.31973) and COD (122.4184).The number of pollution loads (TDS, BOD and COD) are under the assimilation capacity.

KEANEKARAGAMAN TUMBUHAN HUTAN "GUNUNG LUMUT" KABUPATEN PASIR, KALIMANTAN TIMUR Wiriadinata, Harry
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.788

The diversity of forest plants found in three locations in Gunung Lumut Protected Forest, East Kalimantan had been studied in November 2005 in which 252 number of herbarium specimen have been collected, encompassing 194 of herbs and shrubs,33 of trees, 21 numbers of climbers and 4 numbers of orchids.Among the collections like some members of Begoniaceae are interesting for their limited distribution and endemicity.

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