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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Search results for , issue "Vol 10, No 5 (2011)" : 22 Documents clear

KLASIFIKASI VEGETASI GUNUNG ENDUT, TAMAN NASIONAL GUNUNG HALIMUN-SALAK, BANTEN Sambas, EN; Kusmana, C; Prasetyo, LB; Partomihardjo, T
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The research objective is to classify the variety of vegetation types at Mount Endut, Gunung Halimun-Salak National Park. Vegetation sampling was carried out with systematic sampling with random start. Vegetation type at alliance level was determined with vegetation ordination using factor analysis. Four vegetation alliances can be extracted from the ordination. These alliances are Castanopsis acuminatissima-Schima wallichii/Freycinetia javanica (alliance 1); Castanopsis argentea-Dendrocnide stimulans/Schismatoglottis calyptrata (alliance 2); Coffea canephora var. robusta-Quercus lineata/F. javanica( alliance 3) and Paraserianthes falcataria-Coffea canephora var. robusta/Oplismenus compositus (alliance 4). Vegetation alliances form due to their similarity in structure, composition, and physiognomy of vegetation. There are four vegetation associations at alliance 1, i.e. Castanopsis acuminatissima vegetation association, Schima wallichii vegetation association, Garcinia rostrata vegetation association, and Quercus lineata- Eurya acuminata /Freycinetia javanica vegetation association. Alliance 2 has four vegetation associations i.e.Dendrocnide stimulans vegetation association, Coffea canephora var. robusta vegetation association, Castanopsis argentea -Castanopsis acuminatissima vegetation association, and Schismatoglottis calyptrata-Etlingera coccinea vegetation association.There are seven vegetation associations belonging to alliance 3 i.e. Castanopsis acuminatissima vegetation association, Coffea canephora var. robusta vegetation association, Quercus lineata vegetation association, Garcinia rostrata-Schima wallichii vegetation association, Euodia latifolia - Pternandra azurea/Schismatoglottis calyptrata vegetation association, Raphidophora foraminifera vegetation association, and Freycinetia javanica vegetation association. Alliance 4 has five vegetation associations i.e Paraserianthes falcataria vegetation association, Coffea canephora var. robusta vegetation association, Maesopsis eminii/ Erechtites valerianifolia - Clidemia hirta vegetation association, Oplismenus compositus vegetation association, and Clibadium surinamense vegetation association.

PIRAMIDA UMUR DAN PENGELOMPOKAN POPULASI IKAN BONTI-BONTI (Paratherina striata) SECARA SPASIAL DI DANAU TOWUTI, SULAWESI SELATAN Nasution, Syahroma Husni
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Bonti-bonti (Paratherina striata) is an endemiz and vulnerable fish in Lake Towuti and L. Mahalona. The fish population tend to decreasing due to intensive fishing and habitat quality decline. This study was aimed re reveal the age pyramids and the grouping of fish populations as a basis for describing condition and diversty of fish populations. The study was conducted in L.Towuti at five stations. Samples were collected using experimental gillnet with mesh sizedÂ ], Â¾, 1, dan 1Â¼ inches. Meansurement of morphometric characters ofÂ fish consist of 22 characters. Results shows that fish population at each station features a different age pyramid. at station inlent of L. Towuti and Loeha Island, the age pyramid type which is dominated by juveniles a reletively rapid population growth. At station Cape Bakara, outlet of lake Towuti and Beau, the age pyramid type tend to be mederate. This shows the fish population is dominated by medium-size, fish populations in stable condition. Obtained 12 characters that have a strong correlation values. ANCOVA test results showed no difference in morphometric characters between the stations in male and female. Based on ANCOVA test assumed that the Bonti-Bonti at fish station is a population group.Â Â

RESPON PERTUMBUHAN DAN KETERGANTUNGAN Albizzia saponaria (LOUR.) MIQ TERHADAP INOKULASI FUNGI MIKORIZA ARBUSKULA LOKAL SULAWESI TENGGARA PADA MEDIA TANAH PASCA TAMBANG NIKEL Tuheteru, Faisal Danu; Husna, Husna; Arif, Asrianti
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1917

This study that so far has not been conducted yet by others, regarding the effect of a local AMF species from Southeast Sulawesi on the growth of Albizzia saponaria (Lour.) Miq. The effect of AMF to the plant growth depends on the compatibility between host plant and fungi species which was controlled by genotypes of the both symbionts. The aim of this research is to know the growth respons and dependency of A. saponaria to this local AMF grown in post-nickel mining soil. This research was conducted in the Screen House of Forestry Department, Faculty of Agriculture-Haluoleo University since June to September 2010. A completely randomized design with three replications was used. The treatments were no inoculation of AMF (A), inoculated with -1 -1AMF Mycofer inoculum 5 g polybag (B), and inoculated with local AMF inoculum 10 g polybag (C). The seedling parameter observed were height, diameter, number of leaf, total biomass, number of nodule, percentage of root colonization and relative -1 mycorrhizal dependency (RMD). Results showed the increasing growth and biomass with the application of AMF 10 g polybag (C). There was a positive-strongest correlation value (P<0.01) between percentage of root colonization to all of seedling growth parameters, except to the diameter which was only strong correlated (P<0.05). A. saponaria seedling has high dependency level on local AMF for their survival in heavy metal contaminant soil.

KOMPOSISI KIMIA MINYAK ATSIRI PADA BEBERAPA TIPE DAUN TEMBAKAU (Nicotiana tabaccum L.) Nurnasari, Elda; Subiyakto, Subiyakto
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Steam distilation of tobacco (Nicotiana tabaccum L) from 4 different area, Madura (1160 gram), Temanggung (2000 gram),Bondowoso (3528 gram), and Blitar (950 gram) yielded 2,67x10-2 %, 6,93x10-2 %, 0,8428%, and 0,0632 % essensial oil respectively.The oils then analyzed using GC-MS methods. The results identified 30 components from the Maduraâs tobacco, 11 components from the Temanggungâs tobacco, 67 components from the Bondowosoâs tobacco, and 20 components from the Blitarâs tobacco.The major essential oil compounds was neophytadien.

INDUKSI KALUS DAN EMBRIOGENESIS SOMATIK IN VITRO PADA LAMTORO (Leucaena leucocephala) Sapsuha, Yusri; Soetrisno, Djoko; Kustantinah, Kustantinah
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

This research is aimed to determine the effect of various concentrations (0, 1, 2, 3, 4 and 5 mg/l) of 2,4-D (dichloropenoxy acetic acid) on callus induction of Lamtoro (Leucaena leucocephala) and its somatic embryogenesis stimulated using different concentrations of 0, 0.5, 1.0, 1.5 and 2.0 mg/l of growth regulators, namely NAA (Naphthaleneacetic acid) and kinetin. Percentage of callus were measured and somatic embryogenesis from callus were subjected to description analysis. The results showed that callus percentage were varied from 29.34% (1 mg 2,4-D/l) at first week (white color and crumb) to 83.67% (4 mg 2,4-D/l) at fourth week (white yellowish color and compact), and embryo somatic varied from 19.33Â±2.52 (2 mg NAA and 0 mg kinetin/l) to 81.33Â±11.50 (1.5 mg NAA dan 2.0 mg kinetin/l). It can be concluded that optimum callus induction (83.67%) was recorded when concentration of 2,4-D was given at the rate of 4 mg/l. The largest number of embryos in concentration of 1.5 mg/l NAA and 2.0 mg/l kinetin, and embryo somatic 81.33Â±11.50.

KARAKTERISASI DAN STUDI STABILISASI a-AMILASE Bacillus licheniformis TVII.6 MENGGUNAKAN BAHAN ADITIF Lestari, Puji; Richana, Nur; Rosmimik, Rosmimik
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The limited stability of enzyme during long-term storage attributes to its reduced function. In this study, a-amylase from Bacillus licheniformis TVII.6 were formulated with different kind of additives for storage stabilization and better performance. Simultaniously, some minerals and calcium ion were applied to elucidate the inhibition and activation effects to a-amylase. Crude enzyme which was prepared by aceton precipitation was used for this stability test. It demosntrated that 10% of mannitol in citrate phosphate buffer gave the highest residual activity after 3 months of storage (98.5%). Calcium ion influenced the thermal stability of a-amylase and it gave optimum activity at 5 mM CaCl , thus the stability increased from 76.0%/90 C/2 hours to 114.8%/90 C/22 hours in comparison without calcium ions. Calcium ions (5 mM CaCl ) on the stability of a-amylase at 4 C also produced the 2highest residual activity, which remained 100% during 48 hours of incubation. Chemical compounds like FeSO , Na CO and EDTA 4 2 3acted as inhibitors, while (NH ) SO , CuSO , CoSO , MgCl and K HPO did not inhibit activity of a-amylase. NaOH and MnCl 4 2 4 4 4 2 2 4 2 compounds at low concentrations (2 mM) did not inhibit the enzyme but at 10 mM became a-amylase inhibitors. This amylase stability information is very important as a consideration when applying and storing the enzyme, thereby reducing the degradation a-amylase activity.

EKSPLORASI DAN IDENTIFIKASI MIKORIZA INDIGEN ASAL TANAH BEKAS TAMBANG BATUBARA Margarettha, Margarettha
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1923

Attempts to recovery the degraded soils are applied with a friendly environment agricultural concept, such as bioremediation by potential indigenous microorganism like mycorrhyza. The objective of this research is to explore and identify indigenous mycorrhyzas of ex-mining coal soil. Soil samples were obtained as random sampling from top soil and stock pile (depth 0- 30 cm).Furthermore, identifying and trapping of mycorrhyza were also studied. The results obtained 13 types of indigenous mycorrhyzas i.e. Glomus sp. with 9 spore types, Acaulospora sp. with 3 types of and 1 type from Enthrospora sp.

PATOGENESITAS Streptococcus agalactiae DAN Streptococcus iniae PADA IKAN NILA (Oreochromis niloticus) Daenuri, Dudung; Sinaga, Walson Halomoan
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i5.1915

The objectives of this study are to test the pathogenicity of Streptococcus agalactiae and Streptococcus iniae in Nile Tilapia(Oreochromis niloticus) A challenge test was carried out in the Laboratory of Semarang Fish Quarantine. The method used in this study was the Completely Randomize Design(CRD) with three bacterial treatments Streptococcus agalactiae, Streptococcus iniae and control, three dose Â 107 ,108 , 109 with three replications for each treatment. The observed parameters include identification and reisolation of Streptococcus agalactiae, Postulat Koch test, survival rate (SR), and clinical symptoms. The result of this research showed that the survival rate of Nile Tilapia which has challenged by Streptococcus agalactiae was lower than that of Nile Tilapia which has challenged by Streptococcus iniae.

KERAGAAN PERTUMBUHAN HIBRIDISASI EMPAT STRAIN IKAN MAS Ath-thar, MH Fariduddin; Prakoso, Vitas Atmadi; Gustiano, Rudhy
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

One way to support the increase of aquaculture production is to produce superior strains of growth. Carp as one of the potential for these commodities only rely on local strains which have had a decline in quality. For that, the effort to generate superior growth of carp need to be done. One way to do that is by hybridization. We carried out reciprocal crosses of 4 strains of carp Rajadanu, Subang, Majalaya and Kuningan to obtain 16 new strain candidates. Furthermore, growth test was conducted to find out the best candidate of the absolute and specific growth parameters. From these results, it was concluded that the absolute growth in length, weight, and the highest biomass obtained by crosses of SB x RD, the highest length and weight Specific Growth Rate obtained by crosses of RD x MJ. For the highest degree of survival is at the intersection of KN x SB, whereas the highest survival rate in the use of female parent KN. In the dominant male of female survival is the parent SB. The biggest positive heterosis character length growth values obtained by crosses of SB x RD. For the highest weight growth heterosis values obtained by crosses of SB x MJ. On the character of hatching, the highest positive heterosis values obtained by crosses of SB x MJ. The degree of survival of the highest heterosis values obtained by crosses of KN x MJ. On the character of the highest positive heterosis values of biomass obtained by crosses of SB x MJ. As for the character length SGR highest positive heterosis values obtained by crossing two directions of RD and SB. And for the character weight Specific Growth Rate, the highest positive heterosis values obtained by crossing two ways KN and MJ.

HETEROBLASTIC DEVELOPMENT IN SIX SPECIES OF WILD PIPER: Piper baccatum Blume, Piper firmum Blume, Piper majusculum C.DC, Piper miniatum 1 Blume, Piper crocatum Ruiz & Pav. and Piper retrofractum Vahl. IP, Astuti; Munawaroh, E; Rahayu, EMD; Aprilianti, P; Sumanto, Sumanto
BERITA BIOLOGI Vol 10, No 5 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Heteroblastik pada tanaman adalah adanya bentuk karakter morfologi daun yang sangat berbeda pada fase muda dan fase dewasa,yang terus berlangsung dari fase muda sampai dewasa. Telah dilakukan penelitian tentang perubahan morfologi daun pada tiga jenis sirih (Piper spp.) liar yang tumbuh di kawasan Hutan Konservasi Suaka Margasatwa Maninjau Utara-Selatan, Tanjung Raya,Kabupaten Agam, Provinsi Sumatra Barat dan tiga jenis sirih liar koleksi Pusat Konservasi Tumbuhan-Kebun Raya Bogor-LIPI.Dalam penelitian ini, jumlah individu yang diamati dari setiap jenisnya adalah 5 individu muda dan 5 individu dewasa, dengan masing-masing jenis/spesies 5 kali ulangan; sedangkan jumlah spesimen voucher setiap jenisnya adalah 10 spesimen. Data yang dicatat adalah kondisi habitat (di hutan dan di Kebun raya Bogor); lokasi di mana tumbuhan tersebut ditemukan (tepi sungai, tepi jalan, merambat di batu, pohon atau tempat rambatan lainnya). Data lain yang dicatat adalah ukuran daun (lebar dan panjang ),bentuk daun pada fase muda dan dewasa. Selain itu juga dicatat karakteristik morfologi daun lainnya. Enam jenis sirih liar (Piper baccatum, P. firmum, P. majusculum, P. miniatum, P.porphyrophyllum dan P. retrofractum) dari kawasan hutan konservasi Suakamargasatwa Maninjau Utara-Selatan dan Kebun Raya Bogor, memiliki perubahan morforlogi daun pada fase muda dan fase dewasa yang dikenal dengan sebutan perkembangan heteroblastik.

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