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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 30 Documents
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Search results for , issue "Vol 11, No 1 (2012)" : 30 Documents clear
SIFAT ANTIOKSIDAN, KANDUNGAN FENOLAT TOTAL dan FLAVONOID TOTAL EKSTRAK KULIT BATANG MERTAPANG (Terminalia copelandii Elmer) Tri Murningsih
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1884

Abstract

The antioxidant properties of methanolic extract of T. copelandii Elmer bark have been evaluated by using free radical (DPPH) scavenging assay, reduction power and ß-caroten bleaching assay. Ascorbic acid (vitamin C) and “butylated hydroxytoluene” (BHT) were used as positive control or antioxidant standard. Total phenolic content was estimated by using Folin-Ciocalteu reagent and expressed in gallic acid (Gallic Acid Equivalent), while total flavonoid content was estimated by using aluminium chloride reagent and expressed in routine (Routine Equivalent). The result shows antiradical activity of extract on concentration 40 µg/ml was 89.96%, lower than vitamin C (95.61%) but higher than BHT ((52.25%). The reducing power of extract on concentration 50 µg/ml has absorbance value 0.644 lower than vitamin C (0.769), but higher than BHT (0.475). During 2 hours incubation, extract on concentration 50 µg/ml inhibited ß-caroten bleaching 69.46% higher than control negative (35.81%), but lower than BHT (85.99%). Estimation of total phenolic and total flavonoid content showed that extract contained 350.40 µg (GAE)/g extract and 277.50 µg (RE)/g extract respectively. The antioxidant properties may be attributed to the presence of phenolic and flavonoid compounds present in the extract.
KEANEKARAGAMAN JENIS TUMBUHAN PEMAKAN SERANGGA DAN LAJU FOTOSINTESISNYA DI PULAU NATUNA [Diversity of Insectivorous Plants and Its Photosynthetic Rate in Natuna Island] Muhammad Mansur
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.481

Abstract

Studies of insectivorous plants diversity and photosynthesis rate of pitcher plants in Natuna Island was conducted on August 2011, Survey in eleven villages conducted to determine the species found in the visited sites, Bioscientific Portable LCI ADC Photosynthesis System used for measurement of pitcher plants photosynthesic rate, The results shows that there are three families of insectivorous plants founded in study sites, namely Nepenthaceae, Droseraceae and Lentibulariaceae, Six species of Nepenthaceae were found (four species and two natural hybrids) i,e; N. gracilis, N. ampullaria, N. rafflesiana, N. reinwardtiana, N. x hookeriana and N. x trichocarpa. While from family Droseraceae and Lentibulariaceae were found only one species respectively, which is Drosera burmanni and Utricularia bifida. Nepenthes reinwardtiana has the highest rate of photosynthesis namely 21,05 µmol/m /s, followed by N. gracilis (17,66 µmol/m /s), N. rafflesiana (16,6 µmol/m /s), N. x hookeriana (15,84 µmol/m /s) and N. ampullaria (9,96 µmol/m /s).
CEKAMAN OKSIDASI SEL KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARASETAMOL DAN ANTIOKSIDAN (+)-KATEKHIN Heddy Julistiono
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1889

Abstract

In order to more understand similarity of yeast Candida tropicalis with mammalian cells in analgesic drug paracetamol metabolism and toxicity, ability of yeast in the drug metabolism and oxidative response of cells treated with the drug and (+)-catechin was studied. In mammalian cells, paracetamol toxicity is mainly caused by metabolite byproduct of drug metabolism catalyzed by cytochrome P450, a membrane-bound enzyme and peroxidase and a soluble enzyme. Previously it has been shown that paracetamol induced oxidative stress in the yeast cells; and green tea extract protected the cells from oxidation. In this study, it had been shown that paracetamol could be metabolized by yeast cell suspension or cell free extracellular protein, reflecting possibility of role of enzyme that can not be separated from cell and that is soluble, which is common phenomenon in mammalian cell system. Paracetamol of 3 mg/ml increased lipid peroxidation, a marker of oxidative stress. A green tea polyphenol, (+)-catechin of 0.1 mg/ml did not decrease lipid peroxidation content induced by paracetamol. At higher concentration (2 mg/ml), solely (+)-catechin did not increase lipid peroxidation content. Paracetamol or (+)-catechin induced slightly activity of superoxide dismutase enzyme. The data indicated that paracetamol metabolism or toxicity in the yeast may be similar to that of mammalian cells. In this condition, it suggested that (+)-catechin is one of polyphenol green tea that has weak activity of antioxidant and consequently has weak activity of prooxidant. Mechanism of paracetamol toxicity in C. tropicalis is still to be studied with emphasis on the free radical formation and drug metabolism.
KOI HERPES VIRUS SEBAGAI PENYEBAB KEMATIAN MASSAL Cyprinus carpio koi DI INDONESIA [Koi Herpes Virus the Causative Agent of Sporadically Mortality of Cyprinus carpio koi in Indonesia] Sri Oetami Madyowati; Sumaryam Sumaryam; A Kusyairi; Hari Suprapto
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1878

Abstract

Virus was isolated from infected koi Cyprinus carpio koi with KHV and confirmed by polymerase chain reaction (PCR) and transmission electron microscope (TEM). Fish that were naturally living near koi pond such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma did not infected after the cohabitation test. Based on the results KHV was the agent responsible for mortality of million cultured koi in East Java Province Indonesia. Absolute mortality was occurred in koi between 48-72 h post infection, while other cyprinids family was not caused in mortality. Other fresh water fish such as catfish Clarias batrachus, nila Oreochromis niloticus, koki Carrasius auratus, and komet Macropinna microstoma was not infected. Virus-like particles as found in gill ofinfected fish with KHV.
SPATIAL MODEL OF SUMATRAN TIGER (Panthera tigris sumatrae) POTENTIAL HABITAT SUITABILITY IN BUKIT BARISAN SELATAN Suyadi Suyadi; I Nengah Surati Jaya; Antonius B Wijanarto; Haryo Tabah Wibisono
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1885

Abstract

This study describes on development of habitat suitability for Sumatran tiger in Bukit Barisan Selatan National Park (BBSNP). In this study remotely-sensed data set were linked with tiger and it prey survey using camera trap to identify the environmental and human factors that influences the tiger's potential habitat, and to develop a spatial model as well as in BBSNP. All at once, the study showed that the potential model for estimating the tiger's potential habitat suitability could be developed using environmental and human factors. The most significant factors that influence the tiger habitat suitability in Bukit Barisan Selatan National Park are human factors such as distance to road and distance to forest edge and the environmental factor i.e. the number of tiger prey. The verification of the model shows that the model is capable to estimate the probability of the tiger presence having accuracy of approximately 78%. The model shows that there is no significant difference between the predicted data and actual data and having mean deviation less than 10%.
ANALISIS IMUNOGENISITAS PROTEIN GRA1 DARI HASIL KLONING GEN GRA1 TAKIZOIT Toxoplasma gondii Didik T Subekti; WT Artama; SH Poerwanto; E Sulistyaningsih; Yulia Sari
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.482

Abstract

The study was aimed to analyze the immunogenicity of GRA1 protein derived from clone bearing GRA1 genes from local isolate of Toxoplasma gondii. Analysis of GRA1 protein translated from cDNA of GRA1 is very essential in prior to expressed the gene. Analysis of GRA1 protein derived from clone bearing GRA1 genes was performed using several bioinformatics software which are available as standalone or online software such as CLC Bio Workbench series, BioEdit, BESTORF, GENSCAN, FGENES, BepiPred 1.0, CTL Epitope Finder and SignalP. Translation coding sequences of GRA1 gene into GRA1 peptide sequences revealed 190 amino acids with molecular mass of GRA1 approximately 20.159 kD and isoelectric point at 4.43. GRA1 protein also identified several antigenic domains with six domains were known as epitopes for CD8+/cytotoxic lymphocyte and seven domain as epitopes for B lymphocyte. However, GRA1 protein was considered as good antigen but less immunogenic.
ANALISIS PERUBAHAN POLA GENETIK EMPAT GENERASI MANGGIS (Garcinia mangostana L.) BERDASARKAN MARKA ISSR Siti Noorrohmah; Sobir Sobir; D. Efendi
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1880

Abstract

Mangosteen reproduces trough apomixes mechanism, from which the seed develops without fertilization. Mangosteen is an obligate apomicts and it is believed that all of its progenies may have the same genotype as their mother plant. However it was found that genetic variations occured among mangosteen accessions. This research is aimed to study of genetic changing among generations mangosteen. The plant materials used were four generations (P1, P2, P3, and P4) of mangosteen taken from Wanayasa, Purwakarta.The samples were selected according to tree height and taken from each sector (north, east, south, and west). Genetic observations were conducted using Inter Simple Sequence Repeat (ISSR) technique. Ten primers were used to amplify the genomic DNA of mangosteen. It was found that the genetics pattern differed among tree of different age. More variation was observed on the older mangosteen tree.This information may be useful to determine the optimum age of mangosteen for becoming mother plant.
TINJAUAN TENTANG KOPEPODA PARASIT DI INDONESIA [A Review of Parasitic Copepods in Indonesia] Conni Sidabalok
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.456

Abstract

Parasitic copepods are of the most important parasites in the marine environment. They occupy a wide range of hosts ranging from many major groups of invertebrates to the top predators in the sea. Indonesia with vast ocean area has great opportunity to harbour a large numbers of parasitic copepods. However, only few studies of parasitic copepods have been conducted in this region, and most of them are preliminary studies, particularly on mariculture associated copepods. To date, only 70 species of parasitic copepods have been recorded from Indonesian waters. There is a lack of taxonomical approach on these studies, in which some of the specimens were not identified to species level.This highlights the need for taxonomic study and even molecular study of marine parasitic copepods in Indonesia. This paper aims to review the evolution of research on parasitic copepods in Indonesia and to identify areas in need of future research.
ANALISA VEGETASI TEMPAT TUMBUH Hoya purpureofusca Hook.f. DI RESORT SELABINTANA, TAMAN NASIONAL GUNUNG GEDE-PANGRANGO Syamsul Hidayat; Sri Rahayu; Kartika Ningtyas
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1886

Abstract

Research on vegetation, where Hoya purpureofusca Hook.f. grows, has done in the Selabintana Resort, Mount Gede-Pangrango National Park in September 2011. Purposive random sampling was set up for a total of 18 square plots of 10m x 10m, where placed on two different sites. Nine plots were placed in the growing sites of H. purpureofusca, otherwise were placed in other sites. Observation was made in each sampling plots on the number of species at three stages, i.e. seedling, sapling and tree in each sampling plots. The diversity indices (including species richness, heterogeneity, and evenness) were analyzed using Jakcknife index, Margalef index, Menhinick index, Simpson index and Shannon-Wiener index. Species dominance was analyzed by important value index and similarity index whereas species associations was analyzed by chi-square. Based on the analyses, there were differences on vegetation composition and diversity between growing site of H. purpureofusca and non growing site. Schima wallichii (DC.) Korth. is the dominant species that has an important role for the growth of H. purpureofusca.
PENGARUH BEBERAPA PAKET PEMUPUKAN DAN AMELIORASI TERHADAP PERTUMBUHAN DAN HASIL TANAMAN KACANG TANAH (Arachis hypogaea L.) DI KAWASAN PENGEMBANGAN LAHAN GAMBUT Siti Nurzakiah; Koesrini Koesrini; Khairil Anwar
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1882

Abstract

Wetlands in Peatland Development Area has potential for the development of paddy, vegetables and palawija. Development of the commodities are limited or restricted by many limiting production factors such as nutrients, water and plant varieties. The purpose of this study is to know the influence of amelioration and fertilization packages on the growth and yield of peanuts in the swampland peat land development area. The treatments consisted of four amelioration and fertilization package i.e.: (1) Farmer’s package I: 250 kg.ha dolomite, (2) -1 -1 -1 -1 Farmer’s package II: 2.5 t.ha straw compost+18 kg.ha P2O5+300 kg.ha fly ash, (3) Recommendation package: 1.0 t.ha dolomite+22.5 -1 -1 -1 -1 -1 -1 kg.ha N + 36 kg.ha P2O5, and (4) Research package: 2.0 t.ha dolomite+22.5 kg.ha N+22.5 kg.ha P2O5 +30 kg.ha K2O. The result showed that the growth of plants (plant height and number of branches) does not differ between treatments, and the obtained peanut pod yield improvement is 50.45% in the research package treatment compared to Farmer’s package I.

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