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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

ISSN : - EISSN : - DOI : -

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 30 Documents

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Search results for , issue "Vol 11, No 2 (2012)" : 30 Documents clear

POTENSI TRANSMISI VIRUS AVIAN INFLUENZA DARI BABI DAN UNGGAS PADA PETERNAKAN BABI DI WILAYAH TANGERANG, PROVINSI BANTEN [The potential of Transmission of aAvian Influenza Virus from Pig and Bird at the Pig Farm in Tangerang District, Banten Province] Dharmayanti, NLP Indi; Ratnawati, Atik
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pigs have an important role in the ecology of influenza virus since they are sensitive to influenza viruses from human and avian origin. Influenza A virus has a host specificity, although not absolute, so most of the AI virus circulating in various species is only limited to the species, but sometimes there are interactions between different AI virus species or strain. Farming systems that combine a variety of animal species together in the same or around the sites have an important role in the spread of disease and transmission between species. This study is aimed to investigate the cycle of AI virus in order to determine the potential occurrence of viral transmission among species pig and bird at the pig farm that also raising poultry. Influenza virus was identified by methods of RT-PCR and qRT-PCR. The results showed that the novel H1N1 pandemic virus was detected in one pig farm in Tangerang (Banten Province). The AI/H5 virus is also detected in the pig farm that also raises poultry or poultry/pig farmers and located adjacent each other. The AI virus / influence A is also detected in most of the pigs. Detection of AI viruses that infected in pig farm which kept birds or poultry farm around the pigs farm had potential of AI virus transmission from birds species to pig or vise versa. The pigs could serve as a mixing vessel, thus providing opportunities likelihood of reassortant viruses.

RESPON PADI TRANSGENIK CV. NIPPONBARE GENERASI T1 YANG MENGANDUNG GEN Oryza sativa DEHYDRATION-RESPONSE ELEMENT BINDING 1A (OsDREB1A) TERHADAP CEKAMAN SALINITAS [Response of T1 Generation Transgenic Rice cv. Nipponbare Containing an Oryza sativa Dehydration-response Element Binding 1A (OsDREB1A) Gene to Salinity Stress] Santoso, Tri Joko; Apriana, Aniversari; Sisharmini, Atmitri; Trijatmiko, Kurniawan Rudi
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Salinity is one of the abiotic constraints in the cultivation of rice crop. One of the reasons agricultural land becomes saline is due to the intrusion of seawater into the mainland as a result of global climate change. Dehydration-responsive element binding (DREB) gene is a plant -specific transcription factor gene that have important role in regulating plant responses to abiotic stresses, including high salinity. Transgenic rice plants cv. Nipponbare carrying OsDREB1A gene have been generated. However, study of the response of putative transgenic plants to salinity has not been done. The research objective is to study the response of T1 generation Nipponbare-OsDREB1A transgenic rice plants to salinity stress. The result showed that the response of putative transgenic rice Nipponbare-OsDREB1A to salinity stress 25 mM and 150 mM NaCl indicated a level of tolerance varies from highly sensitive to highly tolerance. These variations were possibly occurred because of the segregation state of the T1 generation transgenic rice. Based on damage symptom scoring and PCR analysis provided information that transgenic rice plant cv. Nipponbare-OsDREB1A which showed positive PCR had a very high tolerance to salinity stress 150 mM compared with non-transgenic rice cv. Nipponbare.

OPTIMALISASI KEPADATAN BENIH IKAN MAS (Cyprinus carpio) STRAIN RAJADANU PADA PENDEDERAN DI KOLAM AIR TENANG [Density Optimization of Carp Seed (Cyprinus carpio) Strain Rajadanu in the Nursery in Calm Pool Water] Radona, Deni; Asih, Sidi; Huwoyon, Gleni Hasan
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

National fisheries production is not proportional to the increase of the population now, so that the consumption of animal protein needs are not met.For the sake of national fisheries production there is a need for intensive cultivation technology breakthroughs to spur the density in nursery phase ârajadanuâ carp (Cyprinus carpio) strain. This experiment aim to determine the appropriate density of rajadanu carp growth in the pond at the nursery phase. The average length of tested fishes is 2.02 cm and the average initial weight is 0.25 g maintained in the out door plot pool of Research Installation for Germ Plasm of the Research and Development Division of Freshwater Fisheries, Cijeruk, Bogor measuring 1x1x1 m with density 100, 150 and 200 fishes/m3 .Each treatment was repeated 3 times. Feed used was a commercial feed containing 28% protein given in 2 times a day as much as 5% of the total weight of fish. The results showed that after 40 days, each treatment showed no significant difference (P> 0.05) with absolute length and weight growth of the absolute highest in nursery density of 200 fishes/m3 of 1.30 Â± 0.10 cm and 1.14 Â± 0, 18 cm with a daily growth rate of 1.30 Â± 0.10% and 4.08 Â± 0.55%, and the highest survival at densities 200 fishes/m3 of 92.5 Â± 6.55%.

JAMUR ARBUSKULA DI TAMAN NASIONAL UJUNG KULON [Arbuscular Fungi in Ujung Kulon National Park] Kramadibrata, Kartini
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Study on diversity of arbuscular fungi (AF) of several rizosphere of forest trees and shrub in Karang Ranjang Post, Karang Ranjang Resort,Ujung Kulon National Park (TNUK) was done. Ninety-six soil samples from 23 forest trees were collected for examining AF. Each soil sample was placed into a plastic bag and record the host plant then air dried. Part of the samples were wet sieved by using four type of soil sieves, another part were kept as pot culture in the green house for about six to twelve months, then wet sieved. There were three species of Acaulospora, six species of Glomus, one species of Sclerocytis and one species of Paraglomus associated with forest trees and shrub in TNUK. Most of species recorded from this area are common, except Glomus multicaulis.

KARAKTERISASI GEN PENYANDI PEDIOSIN PAF-11 PADA Pediococcus acidilactici F-11 [Characterization of the Pediocin PaF-11 Encoding Gene in Pediococcus acidilactici F-11] Marwati, Tri; Richana, Nur; Harmayani, Eni; Rahayu, Endang S
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pediocin PaF-11 is a ribosomally synthesized antimicrobial peptide produced by Pediococcus acidilactici F-11. The objectives of this research is to find out the location and the nucleotide sequence of gene, which is involved in the production of pediocin PaF-11. Results showed that the pediocin PaF-11 from the cured cell of P. acidilactici F-11 loss the activity, suggested that the pediocin PaF-11 gene was carried in the plasmid. Agarose gel electrophoresis of P. acidilactici F-11 plasmid DNA with marker ÃÂ»DNA/HindIII showed that pediocin PaF-11 gene was carried in 12 kb plasmid. Amplification pediocin PaF-11 gene from P. acidilactici F-11 showed that uncured P.acidilactici F-11 culture contain plasmid DNA, indicated by amplification of the papA gene (256 bp). Cured P. acidilactici F-11 culture, plasmid eliminated, indicted by no aplicon DNA detected. This result also suggested that pediocin PaF-11 gene in P. acidilactici F-11 was carried in plasmid. Nucleotide of pediocin PaF-11 encoding gene was sequenced The alignment of that nucleotide sequence showed that pediocin PaF-11 encoding gene have the same sequence with pediocin PA.1 encoding gene in P. acidilactici PAC1.0 and P. acidilactici K10 and pediocin AcH encoding gene in P. acidilactici LB 42-923 and P .parvulus ATO77, and pediocin CP2 in P. acidilactici MTCC 5101.

EFEK FERRI SITRAT TERHADAP KEMAMPUAN KHAMIR Candida tropicalis DALAM MEREDUKSI 3-(4,5-DIMETHYLTHIAZOL-2-YL)- 2,5-DIPHENYLTETRAZOLIUM BROMIDE (MTT) Heddy Julistiono; Resti Siti Muthmainah; Adam Adam
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.488

Effect of iron (ferric citrate) on 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyltetrazolium bromide (MTT) reduction in yeast Candida tropicalis was investigated. Reduction of MTT in yeast grown in YMB media containing 5 mM of ferric citrate decreased significantly compared to that of yeast grown containing 0, or 1.25 or 2.5 mM ferric citrate after 24 h incubation. However, there was no difference in cell density among cultures treated with 0 mM, 1.25 mM, and 5 mM ferric citrate. Ferric citrate of 5 mM caused smaller colony when cells were grown on YPDG media. Reduction of MTT in smaller colony cells was weaker than that of with normal size colony. An antioxidant, Epigallocatechin Gallate (EGCG) of 0.01 % could not reverse MTT reduction caused by 5 mM ferric citrate. Since enzymes responsible in MTT reduction are usually located in mitochondrion, the data suggested that, in the condition of 5 mM ferric citrate might cause mitochondrion disorder without killing the yeast cells. The data was in concordance with other studies on other yeast or human cells. However, this study does not show role of free radicals provoked by high level of iron concentration.

KOMPOSISI JENIS DAN POTENSI ANCAMAN TUMBUHAN ASING INVASIF DI TAMAN NASIONAL GUNUNG HALIMUN-SALAK, JAWA BARAT Sunaryo Sunaryo; Tahan Uji; Eka Fatmawati Tihurua
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.493

A study on species composition and threat potential of invasive plant species was conducted in the Gunung Halimun-Salak National Park,Cidahu Resort, West Java. We developed two plots of different altitudes. The result showed that there were four invasive alien species possessing potential threat to the ecosystem and native species in the National Park, i.e. Piper aduncum (Piperaceae, with an Important Value of 20,70); Calliandra calothyrsus (Mimosaceae, IV = 9,11), Austroeupatorium inulaefolium (Asteraceae, IV = 18,77), and Clidemia hirta (Melastomataceae) as shrub. The threats of invasive plants happen in open forest area and could occur from the residential or public places.

KEANEKARAGAMAN FLORA DI TAMAN NASIONAL BUKIT BARISAN SELATAN, PROVINSI LAMPUNG Deby Arifiani; Ridha Mahyuni
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.484

Study on plant diversity by exploration and collection have been carried out at the Bukit Barisan Selatan National Park, Province of Lampung.The study sites was focused on secondary lowland forests of Way Canguk and Sukaraja Atas. The research was aimed to obtain floristic data from such areas that has never been recorded. Flora diversity in these areas is relatively high as represented by 183 plant species from 124 genera and 60 families. It is noted that 3 species are newly for Sumatran flora.

POTENSI TRANSMISI VIRUS AVIAN INFLUENZA DARI BABI DAN UNGGAS PADA PETERNAKAN BABI DI WILAYAH TANGERANG, PROVINSI BANTEN NLP Indi Dharmayanti; Atik Ratnawati
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.489

RESPON PADI TRANSGENIK CV. NIPPONBARE GENERASI T1 YANG MENGANDUNG GEN Oryza sativa DEHYDRATION-RESPONSE ELEMENT BINDING 1A (OsDREB1A) TERHADAP CEKAMAN SALINITAS Tri Joko Santoso; Aniversari Apriana; Atmitri Sisharmini; Kurniawan Rudi Trijatmiko
BERITA BIOLOGI Vol 11, No 2 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i2.494

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