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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 38 Documents
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Search results for , issue "Vol 6, No 3 (2002)" : 38 Documents clear
KOMPONEN KIMIA DAN UJIANTIBAKTERIMINYAK ATSIRIDAUN KI CENGKEH (Urophyllum arboreum (Reinw. ex. Bl.) Korth.) Praptiwi Praptiwi; Yuliasri Jamal; Tri Murningsih
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1218

Abstract

This study was done to determine the chemical compounds and antibacterial activity of ki cengkeh (Urophyllum arboreum (Reinw.ex. Bl.) Korth.) essential oil. Ki cengkeh has already known as medicinal plant in some places in Indonesia. This might be correlated with its chemical compounds. The analysis of chemical compounds of ki cengkeh essential oil was done by GC-MS, while antibacterial test was done by paper disc method against 3 bacteria isolates (Staphylococcus aureus, S. epidermidis and Eschericia coli). The result of GC-MS analysis showed that there were 25 compounds with the concentration more than 1% and carryophylen oxide as the highest compound (12.03%). The highest antibacterial activity was found against S. epidermidis.
PERBANDINGAN KOMPONEN KIMIA PENYUSUN MINYAK ATSIRI SIRIH LIAR {Piper ornatum) YANG BERASAL DARI SULAWESI SELATAN DAN PULAU SERAM DENGAN SIRIH BIAS A (Piper betle) Sri Budi Sulianti; Chairul Chairul
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1223

Abstract

Comparison study on the chemical components of P. ornatum (Piperaceae) volatile oil, collected from Enrekang (South Celebes)and Sepa Village (Ceram Island, Moluccas) had been carried out by water distillation and Gas Chromatography-Mass Spectrometry (GC-MS) analysis. Those plants had exotic performance. The sample from South Celebes had original red color leaves and strong aromatic smell, but one from Ceram Island has green color leaves and weak aromatic smell. The results showed that volatile oil content of P. ornatum from Enrekang has higher than Sepa Village (0.94% and 0.81%), while the volatile oil of common piper (P.betle) was around 0.9-1.2%. GC-MS analysis determined that volatile oil of P. ornatum had 22 chemical components. Both volatile oil indicated similar peak fragmentation, in different intensities. The major component of P. betle and P. ornatum was caryophyllene i.e. 30.01% in the red leaf, 29.41% in the green leaf and 31.05% in P. betle. Some of minor components of P.ornatum were different, from P. betle.
SELEKSI DAN KAPASITASI SPERMATOZOA DENGAN METODE PERCOLL GRADIENT VKYUK FERTILISASI OOSIT DAN PRODUKSIEMBRIO IN VITRO PADA SAPI Endang Triwulanningsih; MR Toelihere; TL Yusuf; B Purwantara; K Diwyanto; JJ Rutledge
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1214

Abstract

This research has been conducted at the laboratory of in vitro fertilization of the University ofWisconsin, USA. These embryos may be used for improving genetic value of Indonesian cattle. Ovaries were collected from slaughterhouse in Wisconsin. Oocytes were matured in TCM-199 medium enriched with FSH 10 \i\lm\, estradiol 17 P lul/ml and 10 % FCS for 20 hours. The oocytes were fertilized in vitro with motile sperm selected and capacitated by using the percoll gradient with 2 ml vs 0.5 ml per layer as treatment A and B respectively. Sperm and oocytes were incubated in fertilization medium (mTALP) for 20 hours. All zygotes were cultured in CRlaa medium up to btastocyst stage and were fed with serum 5 \iV 50 )j.l in culture medium on day 6. Percentages of cleavage, morula, blastocyst, expanded blastocyst, unfertilized and degenerated ova in this study were 86.3 vs 91.6 %, 53.3 % vs 75.9 %; 32.6 % vs 63.4 %; 21.1 % vs 33.0 %; 13.7 % vs 8.4 %, 32.9% vs 15.6 % for treatment A (n=1007) vs B (n=1055), respectively. Based on result of this study, it is concluded that the best method for IVP (in vitro production) of cattle embryos is using percoll gradient with 500 ul per layer.
PENGEMBANGAN TEKNOLOGI PANEN HUJAN DAN ALIRAN PERMUKAAN UNTUK MENEKAN RESIKO KEKERINGAN DAN MENINGKATKAN PRODUKTIVITAS LAHAN (Studi kasus di Sub DAS Bunder, Kabupaten Gunungkidul, Provinsi DIY) Gatot Irianto; Nurwindah Pujilestari
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1228

Abstract

Limited water supply on dry season at dry terrain,cause plantation index and plant productivity low. Mean while yearly water volume are potentially enough for twice plant even more if they manage well. Because of that reason using and developing water resource by rainfall and run off harvesting technology, increasing productivity and maintain dry terrain farm operations system at watershed system are approximation strategy needed to put into practice. That strategy can put into practice through hydrologist characteristic modification at river flow area with escalating capacity and capability watershed distribution to minimalist inundation risk and dried out at dry season. Research result at sub watershed Bunder, Wonosari, DIY by way of building two level dam at river bed (hydrology network) can producing 11.6% (channel reservoir 1) and 18.4% (channel reservoir 2) surface flow from existing river-bed and this result can supply water irrigation at least 3 times in planting season rice-secondary crop- secondary crop). If cropping pattern change to rice-rice-secondary crop then run off harvest need to increase become 100% at upper subwatershed (channel reservoir 1) and 19% at lower subwatershed, with condition at MK 1.irrigation area that can be plant with rice only 0.19 ha and the rest of it can be plant with peanut. Rice productivity at rice field are 2.4 ton/ha.
ISOLASI, SELEKSI DAN OPTTMASI PRODUKSI PROTEASE DARIBEBERAPAISOLAT BAKTERI Elidar Naiola; Nunuk Widhyastuti
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1219

Abstract

Thirty-seven out of sixty-one bacterial isolates from various sources of samples were screened for protease production. The isolate of ISO PL3 could produce the highest enzyme activity, and it was used as a standard bacterial strain in this observation. For any reason,we implemented ISO PL2 to study the optimum condition for producing bacterial protease. Result shows that the maximum protease activity was obtained in a medium containing 100 gram of rice brand in a liter tofu liquid waste. The optimum for incubation was 4- 6 days (agitation of 130 rpm at room temperature) and pH 5.0 - 6.0. After cultivating on this liquid medium, the maximum protease activity of the 2 ISO PL3 was 113,52 x 10' U/ml. From the studies on morphological and physiological characterization, it was indicated that ISO PL3 resemble with the species Bacillus macerans.
EKSPLORASI INFORMASI KEANEKARAGAMAN JENIS, POTENSI DAN PEMANFAATAN TUMBUHAN BAHAN PESTISIDA ALAMI DIPROPINSIJAWA BARAT DAN BANTEN Aseng Ramlan; Iin Supartinah Noer
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1209

Abstract

Pests, diseases and weeds are considered as organisms that limiting the agricultural productivity. Losses of agricultural productivity originated from the attack of these organisms may be up to 40% to 60%; sometimes in several cases failure of harvests occurred. So far, chemical (synthetic) pesticides are used to control the attack of these organisms. Meanwhile, the use of synthetic pesticides in fact, creates many negative effects on both biotic and abiotic environments, such as pollution against terrestrial and aquatic environments, even killing the vertebrates and husbandry animals, predatory insects, and some economic insects (insect species that benefit to the life of man). Due to the losses created by utilization of synthetic pesticides, therefore a number of scientists and farmers initiated to turn their attention to use the natural pesticides (directly originated from plants) under the light of back to nature principle, although this step is seems to be less effective.Some natural pesticides practiced by West Javan farmers are likely almost disappeared and difficult to be found in the field. It is important to mention some species here such as cujete/kalabas (Cresentia cujete), bratawali (linospora tuberculata), bitter yam/gadung (Dioscorea hispida), sambiloto {Andrographis paniculata), sarikaya {Annona squamosa) and so many more. Some weed species are known used by farmers as natural pesticide like saliara {Lantana camara), ki pahit (Tithonia diversifolia), alang-alang (Imperata cylindrical) and badotan {Ageratum conyzoides). This research is aimed to inventory plant species used by farmers as natural pesticides as to reduce the reliance on synthetic pesticides. Methods of approach to the problem are by library studies (journals, proceedings of seminars, notes etc), field survey and interview to farmers of several districts (Kabupatens) in West Java and Banten Provinces i.e. Sumedang, Majalengka, Sukabumi, Garut, Pandegelang and Serang. Result shows that there are still 130 plant species used as natural pesticides, and 42 natural (plant based raw materials) pesticide formulations used by farmers.
KAJIAN PENGARUH SEL IMOBIL Arthrobacter NRRL B-3728 TERHADAP AKTIVITAS DAN STABILITAS ENZIM GLUKOSA ISOMERASE Triantarti Triantarti; Hendro Santoso M
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1224

Abstract

This project focused on the immobilization of glucose isomerase (GI) from Arthrobacter B-3728. The whole cells immobilization technique using glutaraldehyde and gelatine type B220 was used in this research. The optimum time for harvesting Arthrobacter cells was determined before immobilization.The Arthrobacter cells were harvested after 56-72 h fermentation when the GI activity reaching 0.515-0.603 U/ml broth. The best treatment for cell immobilization was found using 5% gelatine when the GI activity reaching 0,888 U/ g immobilized cells. The optimum pH was not changed (pH 8) but the sensitivity to the pH was changed for immobilized cells compared to the free cells. However, the stability of the immobilized cells was lower compared to the free cells for long isomerization process. Further research are still needed for the development of immobilization technique for Arthrobacter B-3728.
STATUS MIKORIZA TUMBUHAN DI GRESSWELL NATURE RESERVE, MELBOURNE, VICTORIA, AUSTRALIA K Kramadibrata
BERITA BIOLOGI Vol 6, No 3 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i3.1215

Abstract

A study on the mycorrhizal status of plants growing in an urban forest reserve which has still patches of remnant native vegetation was conducted in the Gresswell Nature Reserve at Bundoora, Victoria, Australia.The results showed that there were 24 families comprising 53 species.Of the 53 species,32 had AM alone, 15 had AM and ectomycorrhiza,one had ectomycorrhiza alone and five were non-mycorrhizal. There were 26 taxa AM fungi associated with plant roots i.e.,four taxa of Acaulospora, one of Entrophospora, 19 of Glomus and two of Scutellospora.

Page 4 of 4 | Total Record : 38

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