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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 28 Documents
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Search results for , issue "Vol 9, No 3 (2008)" : 28 Documents clear
HUBUNGAN TRANSPIRASI DENGAN HASIL DAN RENDEMEN MINYAK BIJI JARAK PAGAR (Jatropha curcas L.) Bora, Charles Y; Sulistyono, E; Yahya, S; Mahmud, Z
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (525.814 KB) | DOI: 10.14203/beritabiologi.v9i3.779

Abstract

The objective of this research was to evaluate the relationship between transpiration, yield and seed oil content of jatropha/jarak pagar (Jatropha curcas L.). The research was conducted at Cikabayan Research Garden of IPB July 2007-July 2008.The experimental design was Randomized Block Design with two factors and three replications. The first factor was genotype of Jatropha: IP-1A, IP-1M and IP-IP. The second factor was irrigation frequency: 7, 14, 21 and 28 days.The observed variables were evapotranspiration and yield component.The results show that the irrigation frequency of 7 days gave a higher evapotranspiration and transpiration rate than 14, 21 and 28 days irrigation frequencies. The differences affected yield component of each genotipe. The interaction effects between genotype and irrigation frequency were fruit number per plant. The transpiration positively and strongly related to fruits number, seed productions and oil production. Increased transpiration was followed by increasing on fruit number 0.014 (0.112 fruits/1/plant) for IP-1A and 0.024 (0.183 fruits/1/plant) for IP-IP; and increasing by 0.029 g (0.23 g/1/plant) for IP-1A and 0.024 g (0.19 g/1/plant) for IP-IP in seed productions. The transpiration correlated to oil production but not to oil content. Each mm of transpiration increased the oil production 0.0094 g (0.075 g/1/plant) for IP-1A and 0.0073 g (0.058 g/1/plant) for IP-IP. Water use of IP-IP genotype was more efficient than IP-1A in fruits number, but the IP-1A genotype more efficient in seed and oil productions. The differences on growth and yield responses of the jatropha genotypes to transpiration can be used as the model to describe the relationship between transpiration and growth and yield of the plant.
KONSTITUEN KIMIA MINYAK ATSIRI TIGA JENIS TUMBUHAN FAMILI RUTACEAE Jamal, Yuliasri; Sulianti, Sri Budi
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.957 KB) | DOI: 10.14203/beritabiologi.v9i3.784

Abstract

Hydrodistilation process of Feroniella lucida, Clausena harmandiana and Swinglea glutinosa leaves, 0.15%, 0.25 % and 0.20% essential oils were obtained respectively. The GC-MS analysis results shows the essential oil of Feroniella lucida contains w-desil ethanoate (38.23%), 1-decanol (11.01%), 1-decanol acetate (10.82%), and the essential oil of Clausena harmandiana contains a-pinene (12.23%) and copaene (12.40%).On the other hand, the essential oil of Swinglea glutinosa contains isocariophylene (10.09%), 3,3,7,7-tetramethyl-5-(2-methyl-l-prophenyl)-tricyclo 4.1.0.02.4 heptane (21.80%) and nerolidol type 2 (21.34%).O
EKSTRAKSI MINYAK NABATI SECARA FERMENTASI Soeka, Yati Sudaryati
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (417.466 KB) | DOI: 10.14203/beritabiologi.v9i3.789

Abstract

Method of extracting vegetable oil from peanut and soybean cream through enzymatic fermentation was studied. Creams were incubated with respective strains of Bacillus sublilis, Candida rugosa and Pseudomonas aerogenes separately under mild condition that allowed for production of oil at pH 4.5-6.5 and room temperature overnight. A considerably amount of oil could be extracted from media containing coconut and peanut cream, however, none from soybean cream.The oil recovery was about 10 to 30% from approximately 46 to 52% total fat contained in the respective substrates.Those oil were extracted by application of culture filtrate of B. subtilis and C. rugosa exhibited higher linoleic and Iinolenic acid (0.50 to 0.61 % and 0.31 to 0.32% respectively), compared to those were of P. aerogenes lipase (0.20% and 0.13%, respectively).Further investigation was aimed to study the capacity of microbial strains on enzymatic reaction of fatty acid to fatty acid ester by incubating extracted oil in the present of organic solvents.
STUDI ANATOMI DAUN JENIS-JENIS AVERRHOA DI INDONESIA UNTUK MEMPERTEGAS STATUS TAKSONOMINYA Sunarti, Siti; Rugayah, Rugayah; Tihurua, Eka Fatmawati
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (531.526 KB) | DOI: 10.14203/beritabiologi.v9i3.780

Abstract

Leaves anatomy of Averrhoa spp. in Indonesia have been studied for supporting morphological character which used to recognize four species: A. carambola, A. bilimbi, A. leucopetala and A. dolichocarpa. The letter two species have leave crowded terminally or near the top of stem similar to those leave of A. bilimbi, whereas the fruit shape similar to A. carambola.The result of this study indicated that, these four species showed differently in their thickness of lamina and epidermis cell as well.
EKSTRAKSI MINYAK NABATI SECARA FERMENTASI Yati Sudaryati Soeka
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.789

Abstract

Method of extracting vegetable oil from peanut and soybean cream through enzymatic fermentation was studied. Creams were incubated with respective strains of Bacillus sublilis, Candida rugosa and Pseudomonas aerogenes separately under mild condition that allowed for production of oil at pH 4.5-6.5 and room temperature overnight. A considerably amount of oil could be extracted from media containing coconut and peanut cream, however, none from soybean cream.The oil recovery was about 10 to 30% from approximately 46 to 52% total fat contained in the respective substrates.Those oil were extracted by application of culture filtrate of B. subtilis and C. rugosa exhibited higher linoleic and Iinolenic acid (0.50 to 0.61 % and 0.31 to 0.32% respectively), compared to those were of P. aerogenes lipase (0.20% and 0.13%, respectively).Further investigation was aimed to study the capacity of microbial strains on enzymatic reaction of fatty acid to fatty acid ester by incubating extracted oil in the present of organic solvents.
STUDI ANATOMI DAUN JENIS-JENIS AVERRHOA DI INDONESIA UNTUK MEMPERTEGAS STATUS TAKSONOMINYA Siti Sunarti; Rugayah Rugayah; Eka Fatmawati Tihurua
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.780

Abstract

Leaves anatomy of Averrhoa spp. in Indonesia have been studied for supporting morphological character which used to recognize four species: A. carambola, A. bilimbi, A. leucopetala and A. dolichocarpa. The letter two species have leave crowded terminally or near the top of stem similar to those leave of A. bilimbi, whereas the fruit shape similar to A. carambola.The result of this study indicated that, these four species showed differently in their thickness of lamina and epidermis cell as well.
RESVERATROL 10C-GLUKOPIRANOSIDA DAN HEIMIOLA, KARAKTER Hopea gregaria SECARAKEMOTAKSONOMI Sahidin Sahidin; EH Hakim; YM Syah; LD Juliawaty; SA Achmad; LB Din; J Latip
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.785

Abstract

Ten resveratrol oligomers have been isolated from the stem bark of H. gregaria i.e. resveratrol lOC-glucopyranoside (1), heimiol A (2), balanocarpol (3), ampelopsin A (4), hopeaphuran (5), E-viniferin (6), parviflorol (7), a-viniferin (8), hopeaphenol (9), and vaticanoi B (10). The structures of these compounds were determined base on spectroscopic methods including UV, IR, 1-D NMR, 2-D NMR and comparison with the reported data. Implication of the compounds invention is not only strengthen conclusion that Hopea tends to produce resveratrol dimmers but also showed that resveratrol lOC-glucopyranoside (1) and heimiol A (2) are phytochemical markers of. H. gregaria.
INDUKSI KALUS DAN ORGANOGENESIS KULTUR IN VITRO Dendrobium lineale Rolfe Djadja Siti Hazar Hoesen; Witjaksono Witjaksono; LA Sukamto
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.790

Abstract

Callus induction and organogenesis were evaluated in the protocorm like bodies (PLB) culture of Dendrobium lineale Rolfe (Orchidaceae). The globular PLB were cultured in Murashige and Skoog (MS) medium with half strength macronutrients QA MS), supplemented with thidiazuron (TDZ 0,1, 0,5 and 1) mg/1 and 2,4 Dichlorophenoxy acetic acid (2,4-D 0.1, 0.5, 1 and 5) mg/1 as treatments. The media containing 1 mg/1 TDZ and (1 and 5) mg/1 2,4-D was the best treatment for callus initiation (100%). The largest diameter of callus was obtained from 1 mg/1 TDZ (average 3.5 cm). Shoot buds regeneration achieved on 1 mg/1 TDZ (average 41.66 %). However roots regeneration was very low (average 6.66 %) obtained from 1 mg/1 2,4-D. The number of adventitious buds produced from the regenerated shoots on media without plant growth regulator
ANALISIS SENYAWA BIO AKTIF DARI EKSTRAK BUI PICUNG (Pangium edule Reinw.) SEGAR Wibowo Mangunwardoyo; Lily Ismaini; Endang Sri Heruwati
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.781

Abstract

Pangi (Pangium edule Reinw.) seed has long been used traditionally as a preservative agent for fish, especially in remote areas.A study has been conducted on analysis of bioactive compounds of pangi fresh seeds extracts and their fractions.In this study,maceration of fresh seed using water and 50% ethanol was carried out followed by thin layer chromatography (TLC) analysis to see weather the extracts contained tannin. Both extract then separated into their respective fractions using column chromatography.Fractions which had been tested to have highest antibacterial activity were then analysed using gas chromatography mass spectrometry (GC-MS) to assess the active compounds which believe to be a preservative agent.Identification of water and 50% ethanol extract of Pangium edule Reinw.fresh seeds with TLC resulted that tannin were found in those extracts. Furthermore, GC-MS analysis showed that fractions which had been previously tested to have high antibacterial activity contained 9-octadecanoic acid whith similarity index of 89%, and 1,2-benzenedicarboxylic acid whith similarity index of 94-85%.
AKTIVITAS ENZIM PROTEASE DARI Mucorjavanicus YANG DITUMBUHKAN PADA MEDIA TEPUNG SINGKONG {Mannihot utilissima) Abdul Choliq
BERITA BIOLOGI Vol 9, No 3 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i3.786

Abstract

The experiment was conducted to study the activity of protease from Mucor javanicus in cassava flour media. The influence of peptone and incubation time to the production of the enzyme,and the influence of pH and temperature to the activity of protease were studied. The production of protease was conducted in the flask (100 ml) which contain 17.5 g cassava flour and SO ml aqueous media.The species of M.javanicus observed consist of 10 strains. The strain of M.javanicus which has the highest production of protease among the tenth was incubated in cassava flour media with 3 variations of incubation time (4, 7 and 10 days) and 5 variations of peptone concentration (1-5%). The pH and temperature used for protease characterization were pH 4.0-7.0 and 30-70°C, respectively. The results show that the highest protease production with the optimal incubation period 7 days (0.51 U/ml)was M. javanicus M3. The optimal concentration of peptone for protease production was 2 %. The optimal protease activity was reached in pH 5 and temperature 50°C with the value of 4.53 U/ml.

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