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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
 108   109   110   111   112 
PENGUJIAN FERTILITAS PATIN PASUPATI SECARA INTERNAL DAN EKSTERNAL MENGGUNAKAN PATIN SIAM Pangasianodon hypophthalmus (Sauvage, 1878) DAN PATIN JAMBAL Pangasius djambal Bleeker, 1846 Evi Tahapari; Bambang Iswanto
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v16i3.2588

Abstract

Pasupati is a pangasiid catfish resulted from hybridization between female striped pangasiid (Pangasianodon hypophthalmus) and male jambal (Pangasius djamba). Pasupati is known as a superior white-fleshy pangasiid catfish promoted for an export commodity. However, its reproductive characteristic in relation to its fertility has not been examined yet. The present study was conducted to investigate the successful of fertilization between female and male pasupati, reciprocal cross between pasupati with striped catfish, and reciprocal cross between pasupati with jambal catfish.Two to three females and males pasupati were used in the present study. All of breeders were hormonally induced for artificial. The result showed that there is no embryogenesis occurred when sperm from pasupati, striped and jambal catfish were used to fertilize eggs of pasupati catfish. On the other side, sperm enabled to fertilize eggs of striped cat fish producing low percentage of larvae with maternaly similar morphological appearances at juvenile stage. This phenomena showed that spontaneous gynogenesis occurred. In conclusion, female and male of pasupati cat fish are sterile.
KAJIAN KONSERVASI EBONI Amran Achmad
BERITA BIOLOGI Vol 6, No 2 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i2.1489

Abstract

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CELLULOLYTIC YEAST ISOLATED FROM SOIL GUNUNG HALIMUN NATIONAL PARK* [Khamir Selulotik yang Diisolasi dari Tanah Taman Nasional Gunung Halimun] Atit Kanti; I Made Sudiana
BERITA BIOLOGI Vol 6, No 1 (2002)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v6i1.1173

Abstract

Beberapa khamir tanah yang lermasuk dalam marga Debaryomyces dan Candida diisolasi dari tanah Taman Nasional Gunung Halimun.Kemampuan selulotiknya diuji dcngan menumbuhkannya pada media yang mengandung carboxymethyl cellulose (CMC) sebagai sumber karbon utama. Pertumbuhan biomassa dan pH diikuti selama kultivasi. Pengamh penambahan glukosa terhadap aktivitas selulolitik juga dipelajari.Dari 23 isolat yang diuji, 19 termasuk khamir selulolitik, dan 1 isolat yang mempunyai kemampuan tertinggi dipelajari karakterisktik ezymatik sclulasenya. Penambahan glukosa menstimulasi pertumbuhan sel dan menyebabkan kebutuhan sel akan glukosa bertambah, dan sclanjutnya memacu sintesa ensim. Dari isolat-isolat yang diuji, isolat Debaryomyces S-6 mempunyai aktifitas paling tinggi yaitu sebesar 12 UI pada 96 jam inkubasi. Selama waktu kultivasi pH meningkat dari 6,5 menjadi 7. Adanya khamir yang menghidrolisa selulosa mengindikasikan khamir memegang peranan penting dalam transformasi bahan organik dalam tanah.
IDENTIFIKASI VIRUS PENYAKIT JEMBRANA PADA SAPI BALI MENGGUNAKAN PENANDA MOLEKULER GEN env SU Indriawati Indriawati; Endang Tri Margawati; Muhammad Ridwan
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v12i2.534

Abstract

Up to present, detection of Jembrana disease virus has been identified through serological test. Advances in molecular biology has enabled to detect Jembrana disease virus earlier, quicker and more accurate by application of molecular markers.The aim of this study was to identify Jembrana disease by using molecular marker of env SU gene in Bali cattle.Total RNA of Jembrana disease virus (7732bp) was collected from spleen of Bali cattle suspected Jembrana disease by using RNEasy Protect Mini Kit (QIAGEN). A pair of specific primers was designed from Jembrana viral genome (env SU) that accessed through a GenBank with Accession Number of U21603.A kit of Access Quick RT-PCR System (PROMEGA) was used for Reverse-Transcriptase-PCR (RT-PCR). The RT-PCR products were visualized on 2% agarose gel.The result showed a single band with the size of ± 900bp in all samples. This size indicated that env SU gene was existed in the examined spleen samples. This finding suggests that a molecular marker could be used accurately to identify the env SU gene in JDV of Bali cattle.
KOMPOSISI JENIS KEPITING {Decapoda: Brachyura) DALAM EKOSISTEM MANGROVE DAN ESTUARI, TAMAN NASIONAL BALI BARAT Dewi Citra Murniati
BERITA BIOLOGI Vol 10, No 2 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i2.1980

Abstract

Mangrove and estuary ecosystem has highly biodiversity of benthic fauna. Crabs are the common benthic fauna found in this ecosystem. West Bali National Park is one area in Indonesia that has a good condition of mangrove and estuary ecosystem.The present study was done to provide data of the species composition of crabs in West Bali National Park. Sampling was undertaken on June 2009 in Teluk Gilimanuk, Teluk Terima and Teluk Banyuwedang. From a total 411 of crabs specimens, the highest species number was found in Teluk Terima.
BEBERAPA JENIS KERANG KIJING (SUKU: UNIONIDAE) DAN KEONG DI TEPI PERAIRAN SUNGAI RENGAS DAN SUNGKAI, PROPINSI JAMBI RISTIYANTI M. MARWOTO
BERITA BIOLOGI Vol 3, No 7 (1987)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i7.1314

Abstract

RISTIYANTI M.MARWOTO. 1987.Some fresh water mussels family L'nionidae and gastropods from Rengas and Sungkai Rivers in Jambi province. Berita Biologi 3(7): 306 -309.Four species of unionids were found in Rengas river; of these Pseudodon vondenbuschianus,Contradens ascia dimotus,Ctenodesma sp.and Rectidens gracilis,while Physunio superbus and C. ascia dimotus were found in Sungkai river.The density of p.superbus was higher than the other species (7 ind/0.09 m ),it seems was affected by the organic detritus and the differences of the substratum sediment.Some of P.superbus were mature with many eggs and glochidia larvae inside the outer gills.The length of this mature mussels is 28 - 56 mm, while the average is 52 mm.The difficulties in finding young Union idae may be due to their size is relatively small it was or caused by the specific biology of these animals.The occurance of some gastropods in Rengas and Sungkai river were also discussed.
GENETIC ANALYSIS OF SOME SPECIES OFPinanga (PALMAE) BY USING ISSR MARKERS Joko Ridho Witono; Katsuhiko Rondo
BERITA BIOLOGI Vol 8, No 1 (2006)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i1.812

Abstract

A genetic analysis of 13 species of Pinanga (Palmae) was conducted by using Inter Simple Sequence Repeat (ISSR) markers. The markers were used in this study belonged to UBC primer set #9 (UBC 801-900) and each primer contains 15 to 22 mer nucleotides.Based on primer screening, nine UBC primers had clear and reproducible polymorphism bands. According to Dice's and Jaccard's similarity coefficients, cluster analysis by UPGMA among the 13 Pinanga species showed two clusters. Cluster A consisted of nine species: P. javana, P. arinasae, P. patula, P. salicifolia, P. coronata, P. scortechini, P. disticha, P. grandis and P. densiflora; and cluster B consisted of four species from five accessions: P. caesia, P. copelandi, P. rumphiana-1, P. rumphiana-2 and P. insignis. The genetic similarity among the 13 species of Pinanga had a correlation with their geographical distribution. In cluster A, all of the accessions are from Sundaland and the adjacent region of Thailand, whereas all of the accessions in cluster B were distributed in the Philippines, Wallacea, and the New Guinea regions. Possibly this genetic similarity was caused by their geographical history and the natural barriers between them. This is the early conclusion was conducted using genetic markers on Pinanga. Further studies such as sequencing (plastid and nuclear ribosomal DNA) and applying more accessions of Pinanga species from broader geographic distributions may provide a better understanding of the relationships. The 1SSR markers application is a simple and quick way to analyze genetic relationships because no prior sequence data is needed, a large number of markers can be generated, and the supplies and equipment required are minimal.
POTENSI ANTIBAKTERI TIGA SPESIES BAKTERI ASAM LAKTAT ASLI ENGGANO TERHADAP BAKTERI PATOGEN DAN PEMBUSUK MAKANAN Sulistiani Sulistiani; Tatik Khusniati
BERITA BIOLOGI Vol 15, No 3 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v15i3.2410

Abstract

Lactic acid bacteria (LAB) strains have been known for their antimicrobial compounds that are important for food industry,including extension of expired time for food products. In this study, LAB isolates were isolated anaerobically from coconut sap using MRSA medium. Antibacterial analysis was carried out using microdilution method on microplate. The antibacterial assay showed that 85 isolates of LAB exhibit antibacterial activities against Escherichia coli, Salmonella enterica, Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogenes, and Bacillus cereus. Molecular identification of 13 physiologically characterized LAB strains based on 16S rDNA sequence determined three species, namely, Leuconostoc mesenteroides (strain EN17-1, EN17-8, EN17-12, EN17-15, EN17-34, EN17-41, EN17-43, EN17-45, EN17-46, EN38-34), Lactobacillus fermentum (strain EN17-2, EN38-44) and L. satsumensis strain EN38-32. The strong and wide broad spectrum antibacterial activity was produced by L. satsumensis strain EN38-32. 
PENGELOLAAN DAN PEMBUDIDAYAAN CENDANA Cornells Tapatab
BERITA BIOLOGI Vol 5, No 5 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i5.1455

Abstract

Masa lalu cendana dihiasi dengan perannya dalam kisah raja-raja, perekonomian antarbangsa, panggung religi maupun PAD Propinsi.Namun peran ini hampir punah oleh berbagai kebijakan yang kurang tepat,yang tidak jarang dilakukan oleh pemerintah sendiri. Pemikiran dan saran untuk mengembalikan cendana berperan kembali seperti di 'zaman keemasannya' dikemukakan.
PERBANYAKAN TANAMAN KRISAN (Chrysanthemum morifolium) MELALUI KULTUR JARINGAN Ita Dwimahyani; S Gandanegara
BERITA BIOLOGI Vol 5, No 4 (2001)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i4.1127

Abstract

Vegetatively propagated Chrysanthemum morifolium has been carried out by tissue culture.Explants derived from shoot-tip of three chrysant genotypes were used for the experiment. Shoot-tips taken from terminal branch and sterilized with 0.05% HgCb for 30 minutes and rinsed with distilled water three times.Shoot-tips were cultured on MS regeneration medium containing 1 mg/1 BAP and 0.02 mg/1 NAA.Shoot regeneration occurred 20 days after cultured.A number of tiny shoots were then transferred to fresh regeneration medium for 3 weeks and regenerated shoots were calculated.Results showed that plant genotypes had different response into plant regeneration. Genotype Ku-Ch had better response compared to other two genotypes which shown from number of plantlets produced by this genotype on the third sub-culture.

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