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INDONESIA
BERITA BIOLOGI
Published by Lembaga Ilmu Pengetahuan Indonesia
ISSN : -     EISSN : -     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Arjuna Subject : -
Articles 2,125 Documents
 186   187   188   189   190 
PENDETEKSIAN BAKTERI Raistonia solanacearum, Yabuuchi et al 1995 MENGGUNAKAN TEKNIK REAKSI POLIMERASE BERANTAI DAN PEMBEDAAN STRAIN MENGGUNAKAN TEKNIK HIBRIDISASI DNA Yadi Suryadi; M Machmud; MA Suhendar
BERITA BIOLOGI Vol 5, No 1 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v5i1.1090

Abstract

Raistonia solanacearum,the bacterial wilt pathogen, has a wide host range and genetic variability.Rapid and sensitive molecular techniques need to be developed for eariy detection and strain differentiation of the pathogen.Molecular techniques such as PCR and DNA hybridization have been succesfully used to detect and identify bacterial plant pathogens including R.solanacearum.These techniques were adopted under Indonesian condition, using purified and crude DNA from infected plant samples.An R.solanacearum specific DNA primer (OH/Y2) was used in the PCR test,and a DNA probe 5a67 were used in the non-radioactive hybridization test.The PCR techniqe could be used to detect R.solanacearum from infected plant samples in less than 5 hours.The DNA hybridization technique was applicable to differentiate strains ofR.solanacearum into three groups based on their DNA profiles.
ISOLASI GEN SITRAT SINTASE BAKTERI Pseudomonas aerugenosa PS2 DARI RIZOSFER POHON KRUING (Dipterocarpus sp.) UNTUK MODEL KONSTRUKSI METABOLISME SEL MIKROALGA BERKARBOHIDRAT RENDAH Dwi Susilaningsih; Asahedi Umoro; Fredrick Onyango Ochieng; Dian Noverita Widyaningrum; Hani Susanti; Hadi Susilo; I Nengah Swastika; Utut Widyastuti
BERITA BIOLOGI Vol 18, No 2 (2019)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v18i2.2967

Abstract

Pseudomonas has the potential ability for production of citrate synthase synthesis. Pseudomonas aeruginosa could synthesize the enzyme of citrate synthase which is most likely compatible with microalgae cell. Pseudomonas aerugenosa can be found in the rhizosphere of Kruing (Dipterocarpus sp., Dipterocarpaceae). This bacteria is commonly used in agriculture purposes because it is able to synthesize organic acid such as citric acid. These organic acids are synthesized from a reaction between oxaloacetate and acetyl CoA, catalyzed by citrate synthase (CS) in the tricarboxylic acid cycle (TCA). Rhizosphere as microbial sources was obtained from Kruing (Dipterocarpus sp.), which was collected from ‘Carita’ Research Forest, Pandeglang, Banten, West Java. Citrate synthase gene-specific primers were designed based on citrate synthase gene sequences as depicted in Genbank. The isolation and amplification showed that citrate synthase can be detected and purified from Pseudomonas aeruginosa target and it consists of 1600 bp and encodes 509 amino acids. Based on BLAST (Basic Local Alignment Search Tool) analysis, CS genes that were successfully isolated had 92 % similarity with Pseudomonas aeruginosa type II citrate synthase. This CS gene is expected to be expressed in microalgae metabolism to divert the metabolism of carbohydrate formation into fatty acids. 
PENELITIAN EKOLOGI HUTAN DI PULAU NATUNA: STATUS HARA DAUN DAN SERASAH Edi Mirmanto
BERITA BIOLOGI Vol 14, No 1 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v14i1.1869

Abstract

Forest ecological study with special attention to leaf and litterfall nutrient status has been conducted in several forest types at Natuna Island, Riau Archipelago. Ten plots have been setup which distributed in several forest type areas. Fresh leaf was collected from some dominant tree within the plots, whereas fallen leaves collected from litter traps.Nutrient content especially for N, P, K and Ca analyzed from all combined species of both fresh and fallen leaves.Results of chemical analyses showed that all fallen leaves mineral element concentration, except for Ca,were lower than that in fresh leaf. Presentage of retranslocation of both nitrogen and phosphorus tended to be similar to some earlier studies in several tropical forests. Nitrogen and phosphorus were suspected to be limiting factors of forest growth in this area.
AKTIVITAS ENZIM 2,4-D MONOOKSIGENASE DARI BERBAGAI MIKROBA Nunik Sulistinah
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v4i5.1242

Abstract

Nine cultures both from fungi and bacteria have been selected for testing their 2,4-D monooxygenase activity to degrade 2,4-Dtohlorophenoxyacetic acid (2,4-D). The results showed that all the cultures which were tested grows at 1000 ppm 2,4-D. Three cultures (Trichoderma viride. Asperoillus niqer and Isolat E (unidentified)) of the nine cultures are able to grow at 4000 ppm. T. viride grows well on Minimal Basal Media which contained glucose and 2000 ppm 2,4-D and produced the highest biomass (0.8660 g/l) than the others. The biomass of T. viride grew on MBM (without glucose) and added with 2000 ppm 2,4-D is 0,6520g/l. This indicated that the culture is tolerant to 2,4-D and able to use 2,4-D compound as energy and carbon sources for its growth. But we failed to prove the 2,4-D monooxygenase activity of supernatant of T. viride by measuring the changing of pH-value in the 2,4-D breakdown reaction.
PENINGKATAN KADAR LOVASTATIN ANGKAK OLEH Monascuspurpureus KO-KULTUR DENGAN Endomycopsis burtontt Danik D Asadayanti; B Sri Laksmi Jenie; Harsi D Kusu aningrum; Novik Nurhidayaf
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i3.746

Abstract

Lovastatin is a bioactive material of statin groups and has been used to reduce cholesterol through inhibiting HMG Co-A reductase enzyme activities. Three indigenous strains of Monascus purpureus and three mutans were used in this study produced lovastatin at the range of 0,1 - 1,42%. The objectives of this study were to increase lovastatin productions by co-cultured with several concentrations of Endomycopsis burtonii. M. purpureus (10 cfu/ml) was co-cultured with various concentrations of E. burtonii (10M0 ) cfu/ral at three different feeding times (day 2, 4, and 6). Feeding times and concentrations of E. burtonii significantly increased production of lovastatin by four strains of M. purpureus (JMBA5K, AID, JMBA and TOS). The highest production of lovastatin was achieved by M. purpureus TOS co-cultured by E. burtonii at 10 cfu/ml added at day 6. Expression of the genes that responsible for lovastatin production were analyzed by PCR and RT-PCR method. The phenotypic character of M. purpureus TOS with high lovastatin production was conformed by the high intensity of its gene expression.
KEANEKARAGAMAN Begonia (BEGONIACEAE) DARI KAWASAN GUNUNG WATUWILA DAN GUNUNG MEKONGGA, SULAWESI TENGGARA Deden Girmansyah
BERITA BIOLOGI Vol 10, No 1 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i1.2048

Abstract

A taxonomic study of the genus Begonia Blume (Begoniaceae) in South East Sulawesi (Mt. Mekongga and Mt. Watuwila) were conducted based on morphological characters. Six species of Begonia were discovered. Three previously known species (Begonia aptera Blume, B. flacca Irmschr. and B. hispidissima Warburgh.), two new species and one variety were proposed and on going for publication. Some colour pictures were displayed and potential uses of each species are presented.
PERBANDINGAN VEGETASI TUMBUHAN BAWAH DI BAWAH EMPAT JENIS TEGAKAN HUTAN TANAMAN DI CANDIKUNING, BALI MUSTAID SIREGAR
BERITA BIOLOGI Vol 3, No 7 (1987): (Supplement)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v3i7.1378

Abstract

MUSTAID SIREGAR. 1987.The comparison of ground vegetation under four forest plantations in Candikuning, Bali. Suppl. BeritaBiologi. 3: 45-50.The ground vegetation under four forest plantations of different species in Candikuning (Altingia excelsa, Manglietia glauca, Agathis dammara, Pinus merkusiij have been investigated by using quadrat method. The results showed that the highest index of similarity was 53,06% between A. dammara and P. merkusii plantations, and the lowest index of similarity was 18,21% between A. excelsa and P. merkusii plantations. The species composition and community stability of these ground vegetations under "each plantation differed. The community of ground vegetation under A.excelsa plantation was more diverse and stable than the others. The ground vegetation under M. glauca, A.dammara and P.merkusii plantations were dominated by herbs, while under A. excelsa plantation were dominated by seedling of trees, that originated from A. excelsa stand and others surrounding tree species.
POLA PERSARANGAN CURIK BALI (Leucopsar rothscliildiStresemann, 1912) DAN KERAB ATNYA DI TAMAN NASIONAL BALI BARAT Mas Noerdjito
BERITA BIOLOGI Vol 7, No 4 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v7i4.1050

Abstract

Bali myna (Leucopsar rothschildi Stresemann, 1912) was reported as an endemic bird from western part of Bali Island, and also as endangered species.The species lived in the lowland forest and used tree holes made by other animals as their nests.Besides L.rothschildi, there were two other species of birds found using the holes as their nest in the study site, namely Black-winged starling(Sturnus melanopterus (Daudin, 1800)) and Javan myna (Acridotheres fitscus (Wagler, 1827)).This paper deals with result of research on the competition of those three species of birds in obtaining tree hole for nesting, was carried out in West Bali National Park, Bali. Data demonstrated that there were no differences in the size of nest for the three species of birds, Javan myna lived in the habitat of coconut garden where Bali myna did not occupy it, and the habitat of Black-winged starling was overlapped with Bali myna, but Bali myna preferred living in denser vegetation. The reproduction of the three species of birds were dependent on the occurrence of other birds species belonging to Picidae and Capitonidae who were able to make holes on trees.
KERAGAMAN BEBERAPA TUMBUHAN CIPLUKAN (Physalis spp.) DI LERENG GUNUNG KELUD, JAWA TIMUR Nugraheni Hadiyanti; Supriyadi Supriyadi; Pardono Pardono
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v17i2.3238

Abstract

Ciplukan (Physalis spp.) is a wild plant which is that potentially used as a medicinal plant. Information related to the diversity of Physalis spp. in Indonesia is limited. The objectives of the research was to identify the diversity of wild Physalis sp. on Mt. Kelud, East Java based on morphological characters, total protein profiles, and flavonoid compounds. The research was conducted by survey on some gradients viz. 200–400, 400–600, 600–800, 800–1,000 and >1,000 m above sea level. Identification of morphological characters was focused on root, stem, leaf, flower and fruit covering 16 characters for scoring. Identification of total protein profile was performed by SDS-PAGE method and the flavonoid compound was analyzed using UV spectrophotometric method based on the colorimetric principle. The degree of diversity of Physalis spp. was determined based on morphological similarity and total protein profiles through cluster analysis. The results showed that Physalis spp. on Kelud was found up to 1,000 m above sea level. Altitude affected to the morphological diversity, total protein profile and the flavonoid compound. Morphological features that play a role in the diversity of Physalis sp. were the direction of growing stems, leaf shape, leaf margin, leaf surface, leaf stalk color, stain color on the inner neck of thr flower, and fruiting calyx color. Expression of total protein P. minima. shows similarity to P. angulata L. Physalis sp. in the low gradient have a higher flavonoid compound as compared to the middle and high gradients. There were two species of Physalis found on the gradient of Mt. Kelud, East Java, namely Physalis minima L. and Physalis angulata L.
LINNAEUS'S LEGACY AND BIODIVERSITY RESEARCH IN SWEDEN - PAST AND PRESENT Per Sundberg
BERITA BIOLOGI Vol 8, No 4(a) (2007)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v8i4(a).1537

Abstract

Tulisan ini menyampaikan empat hal yaitu ringkasan riwayat hidup Linnaeus, sistem klasifikasi menurut Linnaeus dan Darwin,rangkaian DNA dan ilmu sistematik serta ilmu sistematik mutakhir dan penelitian keanekaragaman hayati di Swedia. Linnaeus yang dilahirkan 300 tahun lalu di Swedia selatan, sangat terkenal karena mengenalkan sistem klasifikasi dan penggunaan dua nama atau binomial untuk penamaan spesies. Sistem penamaan binomial sampai saat inf masih digunakan. Penggunaan sistem binomial untuk penamaan tumbuhan dimulai dengan diterbitkannya buku 'Species Plantarum* pada tahun 1753. Pada binatang, sistem ini pertama kali dikenalkan dalam edisi kesepuluh 'Systema Naturae' pada tahun 1758, yang menjadi titik awal untuk tatanama zoology 'the Code of Zoological Nomenclature'.Sistem klasifikasi menurut Linnaeus bertujuan untuk menyusun keragaman tanpa ada unsur evolusi. Sekitar 100 tahun kemudian Charles Darwin dalam bukunya berjudul 'the Origin of Species'memberi penjelasan lain tentang keragaman hayati yaitu 'evolusi'. Juga Darwin memberi ilustrasi yang sekarang kita sebut pohon filogenetik. dan menyampaikan pemikirannya tentang sistem klasifikasi alami yang merefleksikan 'silsilah' yaitu klasifikasi dan urutan organisme yang menunjukkan adanya hubungan evolusi. Pergeseran dalam ilmu biologi ini kita kenal sebagai filogenetik atau klasifikasi 'cladistic'. Pada tahun 1950an terjadi revolusi dalam ilmu biologi termasuk sistematika, yaitu dengan ditemukannya molekul DNA seperti yang dijelaskan oleh Crick dan Watson. Untuk ilmu sistematik, hal ini merupakan suatu perubahan dramatis yang memungkinkan merekonstruksi filogeni. Rangkaian DNA menyediakan lebih banyak karakter terutama variasi genetik yang tersembunyi dan juga dapat membandingkan jarak antar takson, yang tidak mungkin dilakukan bila hanya berdasarkan karakter morfologi. Perkembangan yang pesat dalam ilmu biologi molekuler tersebut sangat berpengaruh pada ilmu taksonomi dan sistematik mutakhir. Tehnik ini sangat membantu pekerjaan kita dalam memberi nama organisme di seluruh dunia yang sejak zaman Linnaeus sampai sekarang masih belum selesai. Sampai saat ini baru sekitar 1,7 jutajenis yang sudah diberi nama dan dideskripsi, padahal jumlah jenis seluruhnya diduga mencapai 5 sampai 100 juta. Sebagai upaya, pada tahun 2001 Pemerintah Swedia mendirikan The Swedish Species Information Centre dengan programnya yang disebut Swedish Taxonomy Initiative dan sejak tahun 2005 menyediakan anggaran sebesar US$ 9,5 juta per tahun.

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