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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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STUDI PERTUMBUHAN ANAKAN POHON PADA PETAK PERMANEN DI HUTAN DATARAN RENDAH TAMAN NASIONAL GUNUNG GEDE PANGRANGO [Study of seedling growth at permanent plots in lowland forest of Gunung Gede Pangrango National Park] Sundari, Siti
BERITA BIOLOGI Vol 15, No 1 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Study of seedling growth was conducted at permanent plot in lowland forest of Gunung Gede Pangrango. The aim of this research was to determine the relationship between growth parameters such as number of seedling leaves, diameter, the depth of soil organic material and tree height of each seedling at the permanent subplot 5 x 5 m.The results showed that basal area, diameter, and the depth of soil organic material did not significantly affect on the number of seedling leaves, whereas basal area and the depth of soil organic material significantly affected on seedling diameter at each permanent subplot 5 x 5 m. For the trees height at the permanent subplot, it did not significantly affected on the number of seedling leaves, diameter, basal area and the depth of soil organic material. Diameter seedling and the depth of soil organic material were suspected as the main factors for the growth parameters of seedling growth in this area.

KERAGAMAN DAN KELIMPAHAN JENIS KODOK SERTA HUBUNGANNYA DENGAN VEGETASI PADA LAHAN BASAH "ECOLOGY PARK", KAMPUS LIPICIBINONG Kurniati, Hellen
BERITA BIOLOGI Vol 10, No 3 (2010)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Previous ecological studies have revealed the types of non-forest frog commonly occupying habitats that have been modified by humans are still severely limited. For that purpose the research was conducted in the wetland area of Ecology Park in LIPI Campus Cibinong which is located at S 06" 29 40.2"; E 106Â° 51 06.3" with 165 meters altitude above sea level (asl) over seven months (May-November 2009) by monitoring 14 times during the study period (July-November).The transect method was used to determine the effect of habitat on the diversity and abundance of frog species in the wetland area. Transect was set for 100 meters on one side of the lake where the area has a variety of habitat types. Length of 100 meters transect was divided into 10 sections with 10 meters length for each section, (length of each section was 10 meters). Every 10 meters, the type of habitat, frog species encountered and the abundance of the species were recorded. Environmental data recorded were air humidity, air temperature, water temperature and moon phase (full moon, crescent moon or the dark moon). Ten frog species which belong to 4 families, the Bufonidae (Bufo biporcatus and B. melanostictus), Microhylidae (Kaloula baleata), Ranidae (Fejervarya cancrivora, F. limnocharis, Rana chalconota, R. erythraea, R. nicobariensis and Occidozyga lima) and Rhacophoridae (Polypedates leucomystax) were found.R. erythraea, R. nicobariensis and O. lima were the dominant species and found on every visit. Furthermore, the most dominant species was R. erythraea. The results showed that environmental factors (air temperature, water temperature, humidity and conditions of the moon) did not have an impact on the presence and number of species. Abundance of three dominant species (R.erythraea, R. nicobariensis and O. lima) was strongly linked to vegetation type. Moreover, environmental factors (water temperature, humidity and the condition of moon) affected the abundance of R. nicobariensis in sub transect 2 and 5 where dominated by Eleocharis dutcis ans Leerxia hexandra. On the other hand air temperature affected the abundance of O.lima in sub transect 7, dominated by floating lotus plant Nymphaea lotus.

ANALISIS VARIASI GENETIK Saccharomyces cerevisiae Dl TAHAN ETANOL DENGAN RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) [Genetic Variation Analysis of Ethanol Tolerance Yeast, Saccharomyces cerevisiae Dl by Using RAPD] Julistiono, Heddy; Yulineri, Titin; Hanjono, Sukamto
BERITA BIOLOGI Vol 4, No 5 (1999)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Genetic variations among 3 cultures, which were treated with or without Mn of Saccharomvces cerevisiae D1, were analyzed using RAPD (Random Amplified Polymorphic DNA ) technique. The Mn-treatment of three cultures were as follows: the culture KMn was D1 strain, the culture Mn+ was D1 strain colony survived in ethanol 20%, which was previously treated with 0,5 mU MnSOt and the culture Mn- was a D1 colony survived in ethanol 20% without MnSOi treatment. Polymorphism of total DNA of the three cultures may indicate that mutation may occur in cells which were tolerant to ethanol. The locus or base change was not identified. However, since the oxygen uptake rate of the three cultures at catabolite derepression state were identical,the results suggest that the locus may not be in mitochondrial DNA encoding respiratory chain proteins. The relation between DNA polimorphic and ethanol tolerant cell is still to be clarified.

TINJAUAN TENTANG KOPEPODA PARASIT DI INDONESIA [A Review of Parasitic Copepods in Indonesia] Sidabalok, Conni
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Parasitic copepods are of the most important parasites in the marine environment. They occupy a wide range of hosts ranging from many major groups of invertebrates to the top predators in the sea. Indonesia with vast ocean area has great opportunity to harbour a large numbers of parasitic copepods. However, only few studies of parasitic copepods have been conducted in this region, and most of them are preliminary studies, particularly on mariculture associated copepods. To date, only 70 species of parasitic copepods have been recorded from Indonesian waters. There is a lack of taxonomical approach on these studies, in which some of the specimens were not identified to species level.This highlights the need for taxonomic study and even molecular study of marine parasitic copepods in Indonesia. This paper aims to review the evolution of research on parasitic copepods in Indonesia and to identify areas in need of future research.

MIKROBA ENDOFIT DARI TANAMAN SRIKAYA (Annona squamosa L.) SEBAGAI PENGHASIL ANTIMIKROBA Staphylococcus aureus DAN Candida albicans [Antimicrobial activity of endophytic microbes from sugar-apple (Annona squamosa l.) plant againts Staphylococcus aureus and Candida albicans] Melliawati, Ruth; Sunifah, Sunifah
BERITA BIOLOGI Vol 16, No 1 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Various studies indicated that endophytic microbes lived in the plant tissues and produced antimicrobial compounds. Sugar-apple plant Â (Annona squamosa L) contained alkaloids, cyanogenic glycosides, and flavonoids. The purpose of this reasearch were (1) to determine the endophytic microbes isolated from sugar-apple plant (2) to study inhibiting capabillity of endophytic isolate against Staphylococcus aureus and Candida albicans, (3) to analyze antimicrobial compounds produced by the potential endophytic isolate. Diffusion agar plate methode was used to assessed antimicrobial activity. Antimicrobial compounds were analyzed by Thin Layer Chormatography (TLC) and High Performance Liquid Chormatography (HPLC), compared with erythromycin, metronidazole and tetracycline. Twelve bacterial isolates and 24 fungus were isolated. Selected bacteria, BMC 1.1, showed the biggest clear zone on C. albicans culture on agar medium, meanwhile selected fungi, BTCK 1.1T, formed the biggest colony on S. aureus culture on agar medium. TLC and HPLC analysis showed that the Rf value of BMC 1.1 and BTCK 1.1T chloroform phase fractions was similiar to metronidazole. Metronidazole concentration in C1, C2, Ck1 and Ck2 fraction were 170.98 ppm, 18.27 ppm, 1.51 ppm and 4.14 ppm respectively.

ANALISIS FENETIK JAGUNG RAS LOKAL NUSA TENGGARA TIMUR UMUR GENJAH BERDASARKAN KARAKTER AGRONOMI DAN INTER SHORT SEQUENCE REPEATS [Phenetic analysis of Local Landraces of Early Maturity Maize from East Nusa Tenggara based on Agronomic Traits and Inter Short Sequence Repeats] Yulita, Kusumadewi Sri; Bora, Charles Y.; Arsa, IGB Adwita; Murniningsih, Tri
BERITA BIOLOGI Vol 14, No 3 (2015)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Maize having maturity age less than 80 HST (days after planting) has played an important roles as part of food security system for the people of NTT since they can be harvested earlier than common maize and usually consumed by children. Local landraces of maize are usually heterogenous homozygote due to traditional farming system where the farmers used their own harvested maize for generations resulted in low level of heterogeinity. This study was aimed to assess phenotypic and genetic variability among 12 accessions of local maize on the basis of their agronomic perfomance and Inter Short Sequence Repeats profiles. Two accessions of non- early maturity maize were included as control. Ten agronomic traits and eight ISSRs âs primers were used to perform a phenetic analysis using unweighted pair group method with aritmethical average(UPGMA).Clustering dendrogram based on agronomic characters suggested that all accessions were forming three groups on the basis of their maturity (very early mature, early mature, and intermediate mature).On the other hand, analysis using ISSR profiles resulted on random grouping across the accesions.All accessions of early mature age having taxonomic distance of 0.83 and genetic similarity of 57%. This indicated that even though the landraces were open pollinated which allowed cross pollinations during the flowering time, phenotipic and genotipic variations observed to be considerably low.

INFEKTIFITAS Spodoptera exigua NUCLEOPOLYHEDROVIRUS (SeNPV) YANG DIPERKAYA DENGAN BAHAN PENGAKTIF TERHADAP LARVA Spodoptera exigua Huebner [The Infectivity of Spodoptera exigua Nucleopolyhedrovirus (SeNPV) Enriched with the Enhancer to the Spodoptera exigua Huebner Larvae] Samsudin, Samsudin; Santoso, Teguh
BERITA BIOLOGI Vol 11, No 3 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Spodoptera exigua Huebner nucleopolyhedrovirus (SeNPV) is an entomophathogenic virus of onion caterpillar S. exigua larvae commonly used as bioinsecticide. The major limitations of SeNPV for biocontrol of onion caterpillar is it requires long time for the virus to kill the insect host. This research was aimed to find out the material as phagostimulant to increase the infectivity of SeNPV and to determine the optimum boric acid concentration as an enhancer for SeNPV activities. This research was conducted at laboratory using Cipanas isolate of SeNPV and third instar of S. exigua larvae. Phagostimulant used are 5% ofsucrose, soybean sauces, molases, sugar and turmeric filtrate,and the enhancers used are 0,1%, 1%, 5% and 10% concentration of boric acid. This result indicated that soybean sauces and sucrose increased S. exigua consumption and enhanced the infectivity of SeNPV. When mixed with polyhedra of SeNPV, 1% to 5% sucrose significantly increased S. exigua consumption and increased the infectivity of SeNPV, while 10% sucrose tended to decrease the feeding of S. exigua. Boric acid enhanced the infectivity of SeNPV. However, the increase of boric acid concentration should be restricted maximum at 5% for avoiding the negative impacts on the environment.

SINOPSIS Begonia LIAR DI SUMATERA BARAT [Synopsis of Wild Begonia in West Sumatra] Girmansyah, Deden
BERITA BIOLOGI Vol 16, No 3 (2017)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The diversity of wild Begonia in West Sumatra is not well understood, while the habitat of Begonia has continuedly decreased by human activity or natural disaster. Therefore, some species are seriously undert hreat. Examinations of Begonia spp. were carried out using herbarium specimens from Andalas University Herbarium (ANDA), Herbarium Bogoriense (BO) and some field works in West Sumatra were conducted. A total of 33 species of Begonia were collected and identified as species level and could be used for identied Begonia species from West Sumatra.

PROSES AWAL PEMULIHAN HUTAN GAMBUT KELAMPANGAN-KALIMANTAN TENGAH PASCA KEBAKARAN HUTAN DESEMBER 1997 DAN SEPTEMBER 2002 Simbolon, Herwint
BERITA BIOLOGI Vol 7, No 3 (2004)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Two 1-ha (100m by 100m) plots were established at a peat swamp forest of Kelampangan, Central Kalimantan on May 2002. One plot was established at a forest that has been started to regenerate after forest fire December 1997 (PK) and another one at the unburnt natural forest (HAG). Both plots were separated by artificial canal, about 300m away to southwest (HAG) wards and to northeast wards (PK) of the canal. All trees with stem girth at 130cm height of more than 15cm (or about 4.8 cm in diameter) within the plots were enumerated and measured in May 2002 and re-measured again in May 2003. HAG plot was consisted of 3074 tree individuals grouped into 80 species (Fishersa = 15.02) with total basal areas of 33.19 mTha, dominated by (top five in BA, from higher to lower): Callophyllum canum, Combretocarpus rotundatus, Campnosperma squamatum, Ctenolophon parvifolius and Cratoxylum glaucum. Species with higher number of individual were: C. canum (515 individuals), Cp. squamatum (355), Ct. parvifolius (350), Elaeocarpus petiolatus (183) and Cr. glaucum (125). PK plot consisted of 1158 individuals, 103 species (Fishersa = 27.3) and total basal areas were 7.43 mVha, dominated by: Co. rotundatus, Cratoxylum arborescens, Palaquium gutta, Shorea teysmaniana and Syzygium ochneocarpum. Species with higher number of individuals were C. arborescens (256 individuals), S. teysmaniana (104), Sy.ochneocarpum (50), Horsjieldia crassifolia (47) and Cp. squamatum (46). Based on its tree diameter and growth rate, most of the trees (1102 individuals) within PK plot were grown after forest fire December 1997, while the rest 56 trees with higher stem diameter were escaped from forest fire December 1997, mostly belong to: C. canum, Co. rotundatus, Dyera lowii, P. gutta. Based on the species number and total basal areas, the recovery rate of peat swamp forest at PK plot after first forest fire December 1997 were categorized as very high. In September 2002, PK plot was burnt again while HAG remain unburnt, and in May 2003, only 2 individuals of Dyera lowii were found to produce new leaves after escaped from the fire, one laid stem of Co.rotundatus produced new shoot and one standing dead tree of Cr. arborescens produced sprouts from the base of the stem. Growth rate relative,mortality rate and recruitment rate of trees at natural un-burnt forest of the HAG plot were also discussed.

KAJIAN OZONISASI (O3) TERHADAP KARAKTERISTIK KUBIS BUNGA {Brassica oleracea var. botrytis) SEGAR SELAMA PENYIMPANAN PADA SUHU DINGIN Asgar, Ali; Sugiarto, A T; Sumartini, Sumartini; Ariani, D
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The aims and objectives of this study are to determine the effect of ozonization on the characteristics of cauliflower (Brassica oleraceae var.botrytis) during cold storage.The benefit of this research is to prolong the storage period of cabbage flowers with characteristic and quality be appropriate to consumers. The design used is the Randomized Block Design with 6 replications. The treatments consisted of 0 ppm ozone concentration bear leaves, 1.5 ppm ozone concentration without leaves, 1.5 ppm ozone concentration bearing leaves, and 1.5 ppm ozone concentrations without leaves.The parameters observed were weight loss, hardness, moisture content, vitamin C, heavy metals Hg and Ag,number of microorganisms, organoleptic color, freshness and appearance. The results showed that the best treatment was 1.5 ppm ozoneconcentrations with cabbage flowers and leaves. This was caused by ozone acts in sterilization (ozone can kill a variety of pathogenic microorganisms such as bacteria, viruses and fungi), deodorant (ozone eliminating odors caused by a variety of organic compounds and microorganisms), decoloration (ozone can eliminate organic color), and degradation (ozone can break down various organic compounds and heavy metals).

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