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BERITA BIOLOGI

berita_biologi
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Published by Lembaga Ilmu Pengetahuan Indonesia

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Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Articles 2,125 Documents

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PERAN GAMBUT TERHADAP NITROGEN TOTAL TANAH DI LAHAN RAWA [The Role of Peat on Total Nitrogen in The Wetland Soils] Fahmi, Arifin; Radjagukguk, Bostang
BERITA BIOLOGI Vol 12, No 2 (2013)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Peatland has important role in wetland ecosystem stability. Depletion and disappearance of peat layer lead to loss of potential source of nutrient and disruption of wetland ecosystem stability. The research aimed to study the influence of peat thickness and land hydrological condition on the total nitrogen (N) content in soil, the influence of rapidly or naturally the depletion and disappear of peat layer on total N content in soil, and the influence of hydrological condition on total N content in soil. Total N was observed in June of 2009 and 2010 (transition from wet to dry season), September of 2009 and 2010 (peak of dry season), and January of 2010 and 2011 (peak of wet season). The research was carried out on potential acid sulphate soil (A), peaty acid sulphate soil (B),shallow peat which all of peat layers wereremoved (C), shallow peat which peat layer was partially removed (D), shallow peat (E), moderate peat (F) and deep peat (G). The results showed that total N content increased with increasing of peat thickness, the depletion and disappearance of peat layer reduced total N content in soil, and declining in the groundwater level increased total N content in soil.

POTENSIAL AIR PADA TURGOR LOSS POINT TUMBUHAN HUTAN GAMBUT DALAM KONDISI STRES GENANGAN DIKAWASAN SUNGAI SEBANGAU, KALIMANTAN TENGAH Naiola, B. P
BERITA BIOLOGI Vol 5, No 3 (2000)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Water is one of the main phenomenons dominating the life cycle of biodiversity components in tropical peat land area in Central Kalimantan. However, the exist of water is no longer as a determinant positive factor in the life of plants in that area; instead, water has became a factor creating other problems i.e. flooding stress. This report deals with study on the physiological aspects of tropical peat land plant species. Three species were used namely tumih (Combretocarpus rotundatus),belangeran (Shorea balangeran) dan ramin (Gonystylus bancatus).Employed Pressure-Volume Curve generated from Pressure Chamber, this study were concentrated on the water potential at turgor loss points(?total ??po)in flooding conditions. It is assumed that (?total Â ??po)) is important in explaining the adaptation ability of these plants to flooding stress. Results shows that ( ?total ??po)in tumih was (-1,06 MPa), belangeran (-1,53 MPa) and ramin (-1,51 MPa) were not significant different between the three species; thus they had similar ability in responding to flooding stress. However, turgor pressure of these species were significantly different; thus the ratio of each MPa decrease of turgor pressure of each species against their own (?total Â ??po)were different where tumih (0.73 MPa), balangeran (0,78 MPa) and ramin (0,81 MPa). It is suggested that tumih would reach early turgor loss point than the other two species due to its smaller turgor pressure.Comparisons of (?total ??po)among some tropical species were also presented. Due to the relationship between the three water potential components (total water potential, osmotic potential and turgor pressure),thus (?total ??po)maybe genetically controlled, thus it is worth to encourage for further study on this topic.

FLORA GUNUNG KELIMUTU DAN GUNUNG KELIBARA TAMAN NASIONAL KELIMUTU, PULAU FLORES, NUSA TENGGARA TIMUR Wiriadinata, Harry; Wawo, Albert H
BERITA BIOLOGI Vol 9, No 2 (2008)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v9i2.2027

Flora of Mt Kelimutu and Mt Kelibara, Kelimutu National Park, Flores, Lesser Sunda Islands had been studied in July-August 2007.About 70 species belongs to 67 families of plants were collected. Vegetation is dominated by Casuarina junghuhniana Eucalyptus urophylla forest and mixed mountain forest composed of e.q. Ficus variegata, Prunus arborea, Actinodaphne glomerata, Litsea resinosa, Celtis tetrandra and Engelhardtia spicata. It is very interesting due it has many endemic species such as Agalmyla elongata, Calamus heteracanthus, Saurauia schmutzii and S. verheijernii those are endemic to Flores island and Rhododendron renschianum which is endemic in the Kelimutu National Park. During the exploration a new species of Begonia kelimutuensis (in preparation) was recorded.

KULTUR SEL OTAK DAN MATA IKAN KERAPU (Chromileptes altivelis) UNTUK REPLIKASI VIRAL NERVOUS NECROSIS (VNN) Sumaryam, Sumaryam; Kusyairi, Kusyairi; Oetami, Sri; Suprapto, Hari; de Vries, Garry Cores
BERITA BIOLOGI Vol 10, No 4 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The experiment was deal with propagat cell line from encephalon and retina of grouper Chromileptes altivelis.The cell was isolate propagation of method described following by Chen et al.(1982) from carp and crussian carp fin. The cell line from enchepalon and retina of grouper grew very fast and heavy in medium L-1S.The cell line were then used for viral replication giving,isolated virus with high pathogenicity indicated by mortal effect in short time after administration. The preservation of cell line is in 10% glycerol under -80Â°C for one year. Thawing will be done every 3 months to ensure the viability of the cell.

ISOLASI DAN IDENTIFIKASI Pseudomonas DARI TANAH KEBUN BIOLOGI WAMENA DAN UJI AWAL SEBAGAI AGEN BIOKONTROL Fusarium Latupapua, HJD; Nurhidayat, N
BERITA BIOLOGI Vol 6, No 5 (2003)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Pseudomonas bacteria plays essential role in soil ecology such as decomposer and biological control. The bacteria were isolated on selective media and identified from five soil samples taken within area of Wamena Biological Gardens.There are six species Pseudomonas were indentified based on morphological characters and biochemical reaction.P. striata was found to be common in soil of the area.No pathogen Pseudomonas was indentified in all soil samples. Preliminary study on biological control for fungal pathogen Fusarium indicated that P. fluorescens, P. striata and P. cepacia have ability to inhibit Fusarium growth in vitro.

OXYGEN CONSUMPTION OF ROCK BREAM Oplegnathus fasciatus IN DIFFERENT SALINITY LEVELS AND TEMPERATURE DEGREES [Konsumsi oksigen Ikan Rock Bream Oplegnathus fasciatus pada tingkat salinitas dan suhu yang berbeda] Prakoso, Vitas Atmadi; Ryu, Jun Hyung; Min, Byung Hwa; Gustiano, Rudhy; Chang, Young Jin
BERITA BIOLOGI Vol 15, No 2 (2016)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Rock bream Oplegnathus fasciatus is one of marine fish species with high commercial value in the region of East Asia. However, studies on the metabolism related to environmental factors for this species is still lacking. This study was therefore aimed to assess the effects of salinity and temperature on oxygen consumption (OC) of rock bream (TL: 26.9Â±0.6 cm, BW: 477.3Â±61.9 g) was observed by using respiratory chamber to understand the optimal salinity and temperature for culture of rock bream. Research was conducted in Pukyong National University, Busan, South Korea. Four experimental groups were conducted to measure oxygen consumption (OC) according to salinity (35, 25, 15, and 35?5 psu) and temperature changes (15?20?25oC). The results showed that low salinity exposures tend to decrease OC of rock bream (87.1, 78.3, 66.3, and 58.5 mg O2/kg/h at 35, 25, 15, and 5 psu, respectively). Meanwhile, the oxygen consumption of rock bream increased with increasing water temperatures (35 psu: 64.7, 104.0, and 175.9 mg O2/kg/h at 15, 20, and 25oC, respectively; 25 psu: 45.8, 101.7, and 185.9 mg O2/kg/h at 15, 20, and 25oC, respectively; 15 psu: 29.8, 103.3, and 155.5 mg O2/kg/h at 15, 20, and 25oC, respectively).

PENENTUAN FREKUENSI PEMBERIAN PAKAN UNTUK MENDUKUNG PERTUMBUHAN BENIH IKAN PATIN PASUPATI [Determination of Different Feeding Frequency on The Growth of Patin Pasupati Fingerlings] Tahapari, Evi; Suhenda, Ningrum
BERITA BIOLOGI Vol 9, No 6 (2009)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

The feeding rate and the feeding frequency influencing the growth and survival rate of fish. The objective of this research was to know the proper level of feeding frequency for supporting the growth of patin pasupati (hybride between Pangasius hyphophthalmus and P. djambal).The experiment was conducted in fibre glass tanks located at Research Instalation for Genetic and Freshwater Aquaculture Technology, Sukamandi. Patin pasupati fingerling with average body weight of 2.23 ÃÂ± 0.48 g were stocked in 9 fibre glass tanks, each filled with 30 litres of water.The experimental design was Completely Randomized Design with 3 treatments and 3 replications. The treatments were feeding frequency as follows 1, 3 and 5 times per day. The feeding rate was 8% of total body weight per day.The parameters were specific growth rate, average body weight gain, feed conversion ratio, protein retention,lipid retention, protein efficiency ratio and survival rate. The results of experiment indicated that there were significant different(P<0.05) among treatments for specific growth rate, individual body weight gain, feed convertion ratio,protein retention, lipid retention and protein efficiency ratio.The highest value for specific growth rate (7.03%) and protein retention (54.59%) were found for feeding frequency 5 times per day.The values of feed conversion ratio and survival rate for feeding frequency 5 times per day were 0.83 and 100%, respectively.

BIOLOGI PERBUNGAAN JAHE MERAH ( ZINGIBER OFFICINALE ROSC. VAR. RUBRA [ Floral Biology of Red Ginger (Zingiber officinale Rose. var. rubra) Rachman, Erlin
BERITA BIOLOGI Vol 4, No 4 (1998)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

A study on floral biology of red ginger (Zingiber officinale var. rubra) was conducted to approach the floral characters and natural obstacles affecting their sexual reproduction. In vivo observation was directly carried out at Treub Laboratory, The R & D Center for Biology, LIPI, mainly on final development aspects of flower: flowering process, anthesis periodicity and pattern,and pollen fertility. The results showed that commonly there was one of more than 20 spikelets in a spike anthesized perday. Final development stage began to be distinguishable from 6.00 or 7.00 am of anthesis day. Anthesis was always begin after 02.00 pm where the earliest was occurred at 02.04 while the latest was 02.55 pm and consist at least of three stages as is initiation, half and full anthesis.The initiation stage was remarkable by calyx breakage,divided into two types of breakage: -type A (directly break, 58.33%) and -type B (indirectly break, 41.67%).The type B group tend to haa shorter duration of anthesis than type A.Generally this variety of ginger has some negative floral characters base on fertility and fruit or seed setting ability such as very low in anthesis frequency, relatively snort duration of anthesis and inefficient stigma anther locality.In other side, this variety also has positive characters of flower such as enough pollen fertility (32 - 45%), low level in flower abortion and never closing flower even at the of the anthesis process

REGENERASI TANAMAN PEPAYA HASIL TRANSFORMASI DENGAN GEN ACC OKSIDASE ANTISENSE [Regeneration of Transforman Papaya Plant with ACC Oxidase Antisense Gene] Purnamaningsih, Ragapadmi; Mariska, Ika; Hutami, Sri
BERITA BIOLOGI Vol 7, No 5 (2005)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Papaya is climacteric fruit. As the other climacteric fruit, papaya has hight speed ripening, so papaya fruit can not stored in long period. Genetic enginering is one alternative technology to solve the problem by introducing antisense oxidase ACC gen to the papaya plant genome to get delay ripening characteristic. Success of genetic enginering technology depend on plant regeneration system.There were two ways of plant regeneration: organogenesis and somatic embryogenesis. The aim of this experiment was to induce root formation of papaya planlet which trasformated by ACC oxidase antisense gene.The former experiment showed that explant which transformated by ACC oxidase antisense gene can regenerated to be shoot/planlet with P6 medium.But when the shoot transferred to root induction medium the root was difficult to formed, callus was formed at the base of shoot, the leaves turn to yellow and fall down.Many media formulations were tried in this experiment with different basic medium for root induction and development.MS (1, Vi) DKW (1, A) and WPM (1, Vi) were used as basic media combined with sucrose (2 % and 3 %) and plant growth regulators (kinetin, IAA, and paclobutrazol) adding with some organic compound. Result of the experiment showed that MS Vi + paclobutrazol 0.5 mg/1 induced root formation 80 %, inhibited callus formation and decreased yellowing and falling of the leaves.

CEKAMAN OKSIDASI SEL KHAMIR Candida tropicalis YANG DIPERLAKUKAN DENGAN PARASETAMOL DAN ANTIOKSIDAN (+)-KATEKHIN [Oxidative Stress in Candida tropicalis Treated with Paracetamol and Antioxidant (+)-catechin] Julistiono, Heddy
BERITA BIOLOGI Vol 11, No 1 (2012)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v11i1.1889

In order to more understand similarity of yeast Candida tropicalis with mammalian cells in analgesic drug paracetamol metabolism and toxicity, ability of yeast in the drug metabolism and oxidative response of cells treated with the drug and (+)-catechin was studied. In mammalian cells, paracetamol toxicity is mainly caused by metabolite byproduct of drug metabolism catalyzed by cytochrome P450, a membrane-bound enzyme and peroxidase and a soluble enzyme. Previously it has been shown that paracetamol induced oxidative stress in the yeast cells; and green tea extract protected the cells from oxidation. In this study, it had been shown that paracetamol could be metabolized by yeast cell suspension or cell free extracellular protein, reflecting possibility of role of enzyme that can not be separated from cell and that is soluble, which is common phenomenon in mammalian cell system. Paracetamol of 3 mg/ml increased lipid peroxidation, a marker of oxidative stress. A green tea polyphenol, (+)-catechin of 0.1 mg/ml did not decrease lipid peroxidation content induced by paracetamol. At higher concentration (2 mg/ml), solely (+)-catechin did not increase lipid peroxidation content. Paracetamol or (+)-catechin induced slightly activity of superoxide dismutase enzyme. The data indicated that paracetamol metabolism or toxicity in the yeast may be similar to that of mammalian cells. In this condition, it suggested that (+)-catechin is one of polyphenol green tea that has weak activity of antioxidant and consequently has weak activity of prooxidant. Mechanism of paracetamol toxicity in C. tropicalis is still to be studied with emphasis on the free radical formation and drug metabolism.

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