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All Journal EKSAKTA: Journal of Sciences and Data Analysis
Maulani, Qisti
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Chemical Engineering, Chemistry & Bioengineering Chemistry Earth & Planetary Sciences Materials Science & Nanotechnology

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Production of Protease Enzymes and Bioactive Compounds by Lactic Acid Bacteria (LAB) From Broccoli (Brassica oleracea L.) Using Lactobacillus plantarum Starter Hidayat, Habibi; Maharani, Najlaa Ayu; Maulani, Qisti; Fajarwati, Febi Indah
EKSAKTA: Journal of Sciences and Data Analysis VOLUME 7, ISSUE 1, April 2026
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20885/EKSAKTA.vol7.iss1.art12

Abstract

This study aimed to isolate and characterize Lactic Acid Bacteria (LAB) from broccoli (Brassica oleracea L.) with potential protease-producing activity and probiotic properties. Fermentation was carried out using Lactobacillus plantarum as a starter culture. The obtained LAB isolates were subsequently evaluated for protease activity and analyzed for bioactive compound content. The results demonstrated that LAB isolates obtained from broccoli were capable of producing proteases with significant activity, indicating their potential for biotechnological applications. In addition, the isolates produced bioactive compounds exhibiting antibacterial activity against several pathogenic bacteria, including S. epidermidis, K. pneumoniae, E. coli, S. aureus, S. typhi, and S. pyogenes, as evidenced by the formation of inhibition zones. The isolate demonstrated strong antibacterial activity against all tested pathogens with inhibition zones of 56 mm for S. epidermidis, 30 mm for S. typhi, 12 mm for both K. pneumoniae and E. coli, and 11 mm for S. pyogenes and S. aureus. Acid tolerance assays demonstrated that the LAB isolates survived at low pH, and protease activity assays showed that the isolate produced an enzyme with proteolytic activity of 23.5 mm and 0.0440 U/mL.
Co-Authors Fajarwati, Febi Indah Habibi Hidayat, Habibi Maharani, Najlaa Ayu