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All Journal African Journal of Biochemistry and Molecular Biology Research Open Access DRIVERset African Journal of Sciences and Traditional Medicine Open Access DRIVERset Kwaghe International Journal of Sciences and Technology Open Access DRIVERset African Journal of Clinical Medicine and Pharmacy Research African Journal of Medicine, Surgery and Public Health Research
Ahmed, Maryam Usman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Environmental Science Health Professions Immunology & microbiology Law, Crime, Criminology & Criminal Justice Medicine & Pharmacology Nursing Public Health Social Sciences

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Production and Characterisation of Peroxidase from Aspergillus terrens Isolated from Water Sample in Wukari, Taraba State, Nigeria Timothy, Mgbede; Abah, Moses Adondua; Ayo, Victoria Ifeoluwa; Ahmed, Maryam Usman; Oyibo, Okpanachi Nuhu; Ijeomah, Nkechi Gift; Gbadebo, Adetoyi Moses; A, Ajala-Lawal R.; Najeeb, Aliyu Olamilekan; Morolake, Olabanji Martina; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3667

Abstract

The use of biocatalysts like enzymes in the development of environmentally sustainable processes has been reported as an ecofriendly approach for the current bioeconomy. This research aimed at producing and characterizing peroxidase from Aspergillus terrens isolated from water sample in Wukari, Taraba State, Nigeria. Fungi species from surface water sample obtained in Federal University Wukari, Taraba Nigeria were isolated through serial dilution of peptone water and was cultured in a room temperature in different petri dishes using Potato Dextrose Agar (PDA). A portion of the fungi growth chosen from the best growth of cultured water sample were cut and transferred into the 250 Erlenmeyer flask each containing an acceptable proportion of phosphate buffer, pH 6.0. The screening for peroxidase production was aseptically performed on a plate by inoculating the fermented medium with azur B dye. The appearance of a clear zone indicated a positive result for peroxidase activity. The incubation of the peroxidase enzyme was conducted over eleven days, with the highest enzyme yield observed on the eighth day. The results obtained in this study showed that the effect of pH on peroxidase activity was studied, revealing that the optimum pH for enzyme activity was 7.5. Peroxidase activity was lowest at pH 1. Temperature variation studies showed that the optimum temperature for peroxidase activity was 30°C. Peroxidase activity changed as substrate concentration was varied. Peroxidase activity was revealed to be highest at substrate concentration 1.9 mM. The kinetic parameters for peroxidase were investigated to be: Vmax = 10.57082; Km = 0.244186. The evidence presented in this research has shown that peroxidase can be produced in mass by using of white rot fungi from/around our surroundings and can be explored as a cheap source of peroxidase for industrial and biotechnological purposes. In conclusion, producing peroxidase from local isolates presents an eco-friendly and affordable alternative for industrial applications, contributing to environmental sustainability and public health.
Antidiarrhoeal Activity of Methanolic Stem Bark Extract of Sarcocephalus latifolius in Castor Oil-Induced Diarrhoeal Albino Rats Ahmed, Maryam Usman; Titus, Diowato; Isa, Ruqayya
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3708

Abstract

Diarrhoea remains one of the leading causes of death among children in developing countries characterized by frequent passing of watery faeces. This study eval_uates the antidiarrhoeal activities of Sarcocephalus latifolius in castor oil-induced diarrhoeal albino rats. Thirty (30) albino rats were divided into six groups of five rats each. Except group 1 (control group) which received distilled water, all groups received 0.5 mL castor oil to induce diarrhoea. Group 2 received loperamide 3 mg/kg b. wt. Group 3, 4, 5, and 6 received 100, 200, 300, and 400 mg/kg b. wt. respectively of the methanolic stem bark extract of Sarcocephalus latifolius. Total diarrhoeic faeces, stool inhibition, intestinal fluid accumulation, and intestinal transit was determined to eval_uate the antidiarrhoeal effect of the extract. Total diarrhoeal faeces, intestinal content and intestinal transit reflects significant (p<0.05) decrease with increase in the dose of the extract, group 4 which received high dose (400 mg/kg b. wt.) shows no significant difference when compared with group 2 which received loperamide. Percentage inhibition increases with increase in dose. Methanolic stem bark extract of S. latifolius possess antidiarrhoeal effect on castor oil-induced diarrhoea.
Comparative Study of the Effect of Locally Made Beer (Burukutu and Pito) and Star Lager Beer on Liver Function Parameters Ahmed, Maryam Usman; Wahedi, Lumayu Alexander; Abdulfatai, Ayinla Abayomi; Titus, Diowato; Aliyu, Kamaludden
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.6144

Abstract

Alcohol consumption has a long history in human existence and is implicated as one of the major risk factors in the development of liver diseases. The comparative impact of locally made (burukutu and pito) sorghum-based beer and Star Larger beer on the liver biomarkers of albino rats was investigated. 35 albino rats were grouped into 7 groups of 5 rats each. Group one served as the control group and received only distilled water. Group 2 and 3 received 10 and 20 mL/kg b.wt of pito, groups 4 and 5 received 10 and 200 mL/kg b,wt of burukutu, while group 6 and 7 received 10 and 20 mL/kg of Star Larger beers, respectively, for 21 days after which the liver function parameters were assessed. There was a significant decrease (p<0.05) in albumin level of the treatment groups when compared with the control group. Bilirubin and ALP showed no significant difference (p<0.05) from the control group. There was no significant difference (p<0.05) in ALT levels of burukutu and Start Larger when compared with the control group, however, the groups treated with pito showed a significant increase (p<0.05) when compared with the control group. There was a significant increase (p<0.05) in all the treatment groups when compared with the control group, where group 3, which was treated with the higher dose of pito (20 mL/kg b.wt.) had the highest significant increase(p<0.05). albumin levels of the treatment groups showed a significant decrease (p<0.05) when compared with the control group. The three beers compared are toxic to the liver, with pito having the greatest toxicity.
Co-Authors A, Ajala-Lawal R. Abah, Moses Adondua Abdulfatai, Ayinla Abayomi Adamu, Yusuf Muhammad Akem, Ingwu Joseph Aliyu, Kamaludden Anthony, William Asare, Solomon O. Ayinla, Abdulfatai Abayomi Ayo, Victoria Ifeoluwa Danjuma, Tyem Lawal Emmanuel, Comfort Etuk, Idongesit Gbadebo, Adetoyi Moses Ijeomah, Nkechi Gift Ijimari, Thomas Martin Isa, Ruqayya Iseko, Kingsley Iyoko Isreal, Domasun Istifanus, Dafup Katdel Morolake, Olabanji Martina Najeeb, Aliyu Olamilekan Oyibo, Okpanachi Nuhu Salman, Julius Ishaya Samson, Mary Timothy, Mgbede Titus, Diowato Umaru, Hauwa A. Umaru, Isaac John Wahedi, Lumayu Alexander Yeboah, Genevieve A.