Garuda - Garba Rujukan Digital

Production and Characterisation of Peroxidase from Aspergillus terrens Isolated from Water Sample in Wukari, Taraba State, Nigeria Timothy, Mgbede; Abah, Moses Adondua; Ayo, Victoria Ifeoluwa; Ahmed, Maryam Usman; Oyibo, Okpanachi Nuhu; Ijeomah, Nkechi Gift; Gbadebo, Adetoyi Moses; A, Ajala-Lawal R.; Najeeb, Aliyu Olamilekan; Morolake, Olabanji Martina; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3667

The use of biocatalysts like enzymes in the development of environmentally sustainable processes has been reported as an ecofriendly approach for the current bioeconomy. This research aimed at producing and characterizing peroxidase from Aspergillus terrens isolated from water sample in Wukari, Taraba State, Nigeria. Fungi species from surface water sample obtained in Federal University Wukari, Taraba Nigeria were isolated through serial dilution of peptone water and was cultured in a room temperature in different petri dishes using Potato Dextrose Agar (PDA). A portion of the fungi growth chosen from the best growth of cultured water sample were cut and transferred into the 250 Erlenmeyer flask each containing an acceptable proportion of phosphate buffer, pH 6.0. The screening for peroxidase production was aseptically performed on a plate by inoculating the fermented medium with azur B dye. The appearance of a clear zone indicated a positive result for peroxidase activity. The incubation of the peroxidase enzyme was conducted over eleven days, with the highest enzyme yield observed on the eighth day. The results obtained in this study showed that the effect of pH on peroxidase activity was studied, revealing that the optimum pH for enzyme activity was 7.5. Peroxidase activity was lowest at pH 1. Temperature variation studies showed that the optimum temperature for peroxidase activity was 30°C. Peroxidase activity changed as substrate concentration was varied. Peroxidase activity was revealed to be highest at substrate concentration 1.9 mM. The kinetic parameters for peroxidase were investigated to be: Vmax = 10.57082; Km = 0.244186. The evidence presented in this research has shown that peroxidase can be produced in mass by using of white rot fungi from/around our surroundings and can be explored as a cheap source of peroxidase for industrial and biotechnological purposes. In conclusion, producing peroxidase from local isolates presents an eco-friendly and affordable alternative for industrial applications, contributing to environmental sustainability and public health.

Antidiarrhoeal Activity of Methanolic Stem Bark Extract of Sarcocephalus latifolius in Castor Oil-Induced Diarrhoeal Albino Rats Ahmed, Maryam Usman; Titus, Diowato; Isa, Ruqayya
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3708

Diarrhoea remains one of the leading causes of death among children in developing countries characterized by frequent passing of watery faeces. This study eval_uates the antidiarrhoeal activities of Sarcocephalus latifolius in castor oil-induced diarrhoeal albino rats. Thirty (30) albino rats were divided into six groups of five rats each. Except group 1 (control group) which received distilled water, all groups received 0.5 mL castor oil to induce diarrhoea. Group 2 received loperamide 3 mg/kg b. wt. Group 3, 4, 5, and 6 received 100, 200, 300, and 400 mg/kg b. wt. respectively of the methanolic stem bark extract of Sarcocephalus latifolius. Total diarrhoeic faeces, stool inhibition, intestinal fluid accumulation, and intestinal transit was determined to eval_uate the antidiarrhoeal effect of the extract. Total diarrhoeal faeces, intestinal content and intestinal transit reflects significant (p<0.05) decrease with increase in the dose of the extract, group 4 which received high dose (400 mg/kg b. wt.) shows no significant difference when compared with group 2 which received loperamide. Percentage inhibition increases with increase in dose. Methanolic stem bark extract of S. latifolius possess antidiarrhoeal effect on castor oil-induced diarrhoea.

Comparative Study of the Effect of Locally Made Beer (Burukutu and Pito) and Star Lager Beer on Liver Function Parameters Ahmed, Maryam Usman; Wahedi, Lumayu Alexander; Abdulfatai, Ayinla Abayomi; Titus, Diowato; Aliyu, Kamaludden
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.6144

Alcohol consumption has a long history in human existence and is implicated as one of the major risk factors in the development of liver diseases. The comparative impact of locally made (burukutu and pito) sorghum-based beer and Star Larger beer on the liver biomarkers of albino rats was investigated. 35 albino rats were grouped into 7 groups of 5 rats each. Group one served as the control group and received only distilled water. Group 2 and 3 received 10 and 20 mL/kg b.wt of pito, groups 4 and 5 received 10 and 200 mL/kg b,wt of burukutu, while group 6 and 7 received 10 and 20 mL/kg of Star Larger beers, respectively, for 21 days after which the liver function parameters were assessed. There was a significant decrease (p<0.05) in albumin level of the treatment groups when compared with the control group. Bilirubin and ALP showed no significant difference (p<0.05) from the control group. There was no significant difference (p<0.05) in ALT levels of burukutu and Start Larger when compared with the control group, however, the groups treated with pito showed a significant increase (p<0.05) when compared with the control group. There was a significant increase (p<0.05) in all the treatment groups when compared with the control group, where group 3, which was treated with the higher dose of pito (20 mL/kg b.wt.) had the highest significant increase(p<0.05). albumin levels of the treatment groups showed a significant decrease (p<0.05) when compared with the control group. The three beers compared are toxic to the liver, with pito having the greatest toxicity.

Production and Characterisation of Peroxidase from Aspergillus terrens Isolated from Water Sample in Wukari, Taraba State, Nigeria Timothy, Mgbede; Abah, Moses Adondua; Ayo, Victoria Ifeoluwa; Ahmed, Maryam Usman; Oyibo, Okpanachi Nuhu; Ijeomah, Nkechi Gift; Gbadebo, Adetoyi Moses; A, Ajala-Lawal R.; Najeeb, Aliyu Olamilekan; Morolake, Olabanji Martina; Iseko, Kingsley Iyoko; Istifanus, Dafup Katdel; Umaru, Isaac John
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3667

The use of biocatalysts like enzymes in the development of environmentally sustainable processes has been reported as an ecofriendly approach for the current bioeconomy. This research aimed at producing and characterizing peroxidase from Aspergillus terrens isolated from water sample in Wukari, Taraba State, Nigeria. Fungi species from surface water sample obtained in Federal University Wukari, Taraba Nigeria were isolated through serial dilution of peptone water and was cultured in a room temperature in different petri dishes using Potato Dextrose Agar (PDA). A portion of the fungi growth chosen from the best growth of cultured water sample were cut and transferred into the 250 Erlenmeyer flask each containing an acceptable proportion of phosphate buffer, pH 6.0. The screening for peroxidase production was aseptically performed on a plate by inoculating the fermented medium with azur B dye. The appearance of a clear zone indicated a positive result for peroxidase activity. The incubation of the peroxidase enzyme was conducted over eleven days, with the highest enzyme yield observed on the eighth day. The results obtained in this study showed that the effect of pH on peroxidase activity was studied, revealing that the optimum pH for enzyme activity was 7.5. Peroxidase activity was lowest at pH 1. Temperature variation studies showed that the optimum temperature for peroxidase activity was 30°C. Peroxidase activity changed as substrate concentration was varied. Peroxidase activity was revealed to be highest at substrate concentration 1.9 mM. The kinetic parameters for peroxidase were investigated to be: Vmax = 10.57082; Km = 0.244186. The evidence presented in this research has shown that peroxidase can be produced in mass by using of white rot fungi from/around our surroundings and can be explored as a cheap source of peroxidase for industrial and biotechnological purposes. In conclusion, producing peroxidase from local isolates presents an eco-friendly and affordable alternative for industrial applications, contributing to environmental sustainability and public health.

Antidiarrhoeal Activity of Methanolic Stem Bark Extract of Sarcocephalus latifolius in Castor Oil-Induced Diarrhoeal Albino Rats Ahmed, Maryam Usman; Titus, Diowato; Isa, Ruqayya
African Journal of Biochemistry and Molecular Biology Research Vol 1 No 1 (2024): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v1i1.3708

Diarrhoea remains one of the leading causes of death among children in developing countries characterized by frequent passing of watery faeces. This study eval_uates the antidiarrhoeal activities of Sarcocephalus latifolius in castor oil-induced diarrhoeal albino rats. Thirty (30) albino rats were divided into six groups of five rats each. Except group 1 (control group) which received distilled water, all groups received 0.5 mL castor oil to induce diarrhoea. Group 2 received loperamide 3 mg/kg b. wt. Group 3, 4, 5, and 6 received 100, 200, 300, and 400 mg/kg b. wt. respectively of the methanolic stem bark extract of Sarcocephalus latifolius. Total diarrhoeic faeces, stool inhibition, intestinal fluid accumulation, and intestinal transit was determined to eval_uate the antidiarrhoeal effect of the extract. Total diarrhoeal faeces, intestinal content and intestinal transit reflects significant (p<0.05) decrease with increase in the dose of the extract, group 4 which received high dose (400 mg/kg b. wt.) shows no significant difference when compared with group 2 which received loperamide. Percentage inhibition increases with increase in dose. Methanolic stem bark extract of S. latifolius possess antidiarrhoeal effect on castor oil-induced diarrhoea.

Comparative Study of the Effect of Locally Made Beer (Burukutu and Pito) and Star Lager Beer on Liver Function Parameters Ahmed, Maryam Usman; Wahedi, Lumayu Alexander; Abdulfatai, Ayinla Abayomi; Titus, Diowato; Aliyu, Kamaludden
African Journal of Biochemistry and Molecular Biology Research Vol 2 No 2 (2025): African Journal of Biochemistry and Molecular Biology Research
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajbmbr.v2i2.6144

Alcohol consumption has a long history in human existence and is implicated as one of the major risk factors in the development of liver diseases. The comparative impact of locally made (burukutu and pito) sorghum-based beer and Star Larger beer on the liver biomarkers of albino rats was investigated. 35 albino rats were grouped into 7 groups of 5 rats each. Group one served as the control group and received only distilled water. Group 2 and 3 received 10 and 20 mL/kg b.wt of pito, groups 4 and 5 received 10 and 200 mL/kg b,wt of burukutu, while group 6 and 7 received 10 and 20 mL/kg of Star Larger beers, respectively, for 21 days after which the liver function parameters were assessed. There was a significant decrease (p<0.05) in albumin level of the treatment groups when compared with the control group. Bilirubin and ALP showed no significant difference (p<0.05) from the control group. There was no significant difference (p<0.05) in ALT levels of burukutu and Start Larger when compared with the control group, however, the groups treated with pito showed a significant increase (p<0.05) when compared with the control group. There was a significant increase (p<0.05) in all the treatment groups when compared with the control group, where group 3, which was treated with the higher dose of pito (20 mL/kg b.wt.) had the highest significant increase(p<0.05). albumin levels of the treatment groups showed a significant decrease (p<0.05) when compared with the control group. The three beers compared are toxic to the liver, with pito having the greatest toxicity.

Evaluation of the Antidiarrheal Activity of Aqueous Leaf Extract of Senna surattensis on Castor Oil-Induced Diarrhea in Rats Ahmed, Maryam Usman; Samson, Mary; Adamu, Yusuf Muhammad
African Journal of Sciences and Traditional Medicine Vol 2 No 1 (2025): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v2i1.4535

Diarrheal disease is a major health problem in developing countries. It is the second leading cause of death among children under five globally; accounting for about 9 percent of all deaths. This study was aimed at evaluating the antidiarrheal activity of the aqueous leaf extract of Senna surattensis. The antidiarrheal effect of the extract was evaluated using castor oil-induced diarrhea model. Loperamide was used as the standard drug. Thirty (30) Wistar rats were divided into six (6) groups of five (5) rats each. Groups I and II served as the normal and negative controls respectively, group III as standard treatment and groups IV, V and VI as test groups. The extract was administered orally at three different doses of 100 mg/kg, 200 mg/kg and 400 mg/kg to groups IV, V and VI respectively. The extract exhibited a graded dose-dependent inhibition of castor oil-induced diarrhea. The stool inhibition was highly significant at 100 mg/kg (88.1% inhibition) and maximal at 400 mg/kg (100% inhibition). The maximal effect produced by the extract at 400 mg/kg (100% inhibition) is similar to that of the standard drug (5 mg/kg loperamide) which produced 97.6% inhibition. The extract also exhibited a modest dose-dependent reduction on the distance travelled by the charcoal meal. This extract high doses, significantly decrease the volume of the intestinal content when compared to that of the negative control. The results obtained from this study suggests that the aqueous leaf extract of Senna surattensis have significant antidiarrheal effect on animal models and this finding supports the traditional use of this plant extract for treatment and or management of diarrhea.

Evaluation of the Antidiarrheal Activity of Aqueous Leaf Extract of Senna surattensis on Castor Oil-Induced Diarrhea in Rats Ahmed, Maryam Usman; Samson, Mary; Adamu, Yusuf Muhammad
African Journal of Sciences and Traditional Medicine Vol 2 No 1 (2025): African Journal of Sciences and Traditional Medicine
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/ajstm.v2i1.4535

Diarrheal disease is a major health problem in developing countries. It is the second leading cause of death among children under five globally; accounting for about 9 percent of all deaths. This study was aimed at evaluating the antidiarrheal activity of the aqueous leaf extract of Senna surattensis. The antidiarrheal effect of the extract was evaluated using castor oil-induced diarrhea model. Loperamide was used as the standard drug. Thirty (30) Wistar rats were divided into six (6) groups of five (5) rats each. Groups I and II served as the normal and negative controls respectively, group III as standard treatment and groups IV, V and VI as test groups. The extract was administered orally at three different doses of 100 mg/kg, 200 mg/kg and 400 mg/kg to groups IV, V and VI respectively. The extract exhibited a graded dose-dependent inhibition of castor oil-induced diarrhea. The stool inhibition was highly significant at 100 mg/kg (88.1% inhibition) and maximal at 400 mg/kg (100% inhibition). The maximal effect produced by the extract at 400 mg/kg (100% inhibition) is similar to that of the standard drug (5 mg/kg loperamide) which produced 97.6% inhibition. The extract also exhibited a modest dose-dependent reduction on the distance travelled by the charcoal meal. This extract high doses, significantly decrease the volume of the intestinal content when compared to that of the negative control. The results obtained from this study suggests that the aqueous leaf extract of Senna surattensis have significant antidiarrheal effect on animal models and this finding supports the traditional use of this plant extract for treatment and or management of diarrhea.

Toxicological Evaluation of Aqueous Leaf Extract of Phyllanthus niruri in Wistar Rats Ahmed, Maryam Usman; Ijimari, Thomas Martin; Anthony, William; Etuk, Idongesit; Abdulfatai, Ayinla Abayomi
Kwaghe International Journal of Sciences and Technology Vol 2 No 2 (2025): Kwaghe International Journal of Sciences and Technology
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/kijst.v2i2.6145

Natural products have long been used as therapeutic agents; however, many lack comprehensive scientific evaluation of their toxicity. This study investigates the potential toxicity of Phyllanthus niruri (P. niruri) aqueous leaf extract in Wistar rats. Twenty-five male albino rats were randomly divided into five groups of five animals each. Groups 2 through 5 received daily oral doses of 100, 200, 400, and 800 mg/kg body weight of the extract, respectively, while Group 1 served as the control and received only grower mash and distilled water for 28 days. Toxicological assessment was conducted through hematological profiling and evaluation of liver and kidney function using standard biochemical methods. The results indicated a significant, dose-dependent increase (p<0.05) in the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP), suggesting hepatic stress. In contrast, albumin, creatinine, and urea levels did not differ significantly (p<0.05) from the control group, indicating no observable impairment in renal function. Hematological analysis revealed a significant decrease (p<0.05) in packed cell volume (PCV) and red blood cell (RBC) count, while white blood cell (WBC) count, hemoglobin (Hb) concentration, and mean corpuscular volume (MCV) significantly increased with higher extract doses. These findings suggest that while P. niruri is traditionally regarded as a natural remedy, its aqueous leaf extract may induce hematological and hepatic alterations at elevated doses. Caution is therefore advised in its use, particularly in unregulated or prolonged applications.

The Legalization of Abortion and Its Influence on Youth Sexual Behavior and the Catholic Church: A Case Study of Adamawa State Umaru, Isaac John; Akem, Ingwu Joseph; Asare, Solomon O.; Danjuma, Tyem Lawal; Yeboah, Genevieve A.; Salman, Julius Ishaya; Umaru, Hauwa A.; Ahmed, Maryam Usman
Kwaghe International Journal of Sciences and Technology Vol 3 No 1 (2026): Kwaghe International Journal of Sciences and Technology
Publisher : Darul Yasin Al Sys

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.58578/kijst.v3i1.9401

Although debates on abortion reform have intensified in Nigeria, limited attention has been paid to how partial legalization shapes youth perspectives and sexual and reproductive behaviors within specific sociocultural and religious contexts. This study examines how the limited legalization of abortion influences youth attitudes and behaviors in Adamawa State, with particular attention to demographic, cultural, and institutional factors, as well as the Catholic Church’s response to these changes. A qualitative approach was employed through interviews with health workers, clergy, and youths aged 18–25 from urban and rural areas of Adamawa State. Supplementary data were obtained from community forums, church outreach programs, and health service records, and the data were analyzed thematically to identify patterns in attitudes, behaviors, and institutional responses. The findings indicate a marked urban–rural divide, with urban youths reporting higher sexual activity, greater contraceptive use, and stronger support for reproductive autonomy. The study also found that the Catholic Church’s moral campaigns have had limited influence, particularly among urban youths who perceive religious teachings as less relevant to contemporary realities. In contrast, health workers were viewed as more trusted sources of guidance, while peers and media played a substantial role in shaping youth behavior. The study concludes that the evolving legal and cultural landscape in Adamawa State reflects a broader generational shift toward autonomy and informed decision-making among youths. These findings contribute to understanding the changing relationship between legal reform, religious influence, and youth reproductive behavior, and suggest that more balanced strategies integrating moral guidance, comprehensive education, and supportive policies are needed to promote healthy youth development in a changing society.

Title

Found 14 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 14 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 14 Documents
Search