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All Journal JSMARTech : Journal of Smart Bioprospecting and Technology
Amalia, Nurul Iffah
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Biochemistry, Genetics & Molecular Biology Chemistry Materials Science & Nanotechnology Medicine & Pharmacology Neuroscience

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Preliminary In Vitro Screening of a 70% Ethanol Extract of Matoa (Pometia pinnata) Fruit Peel against Staphylococcus epidermidis and Trichophyton mentagrophytes Using the Disc Diffusion Method Amalia, Nurul Iffah; Rachmawaty, Rachmawaty; Khalidatunnisa, Besse; Hala, Yusminah; Ali, Alimuddin
JSMARTech: Journal of Smart Bioprospecting and Technology Vol. 7 No. 2 (2026): JSMARTech Volume 7, No 2, 2026
Publisher : JSMARTech

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jsmartech.2026.007.02.23

Abstract

Matoa (Pometia pinnata) fruit peel is an agricultural by-product whose activity against skin-associated microorganisms remains insufficiently characterized. This study evaluated a 70% ethanol extract of matoa fruit peel against Staphylococcus epidermidis ATCC 12228 and Trichophyton mentagrophytes ATCC 18748 using the disc diffusion method at 15%, 20%, 25% and 30% (w/v). Chloramphenicol and ketoconazole served as positive controls, while 10% dimethyl sulfoxide served as the negative control. Three technical replicates were performed per treatment using a single extraction batch. Mean widths of the clear zones beyond the discs ranged from 6.33 to 9.33 mm for S. epidermidis and from 14.00 to 18.00 mm for T. mentagrophytes. No clear zone beyond the disc was observed with the negative control. Analysis of variance showed no significant differences among extract concentrations for either organism (p > 0.05). These preliminary findings require cautious interpretation because the replicates were technical rather than biological. Further studies using independent extraction batches and minimum inhibitory concentration testing are needed.
Co-Authors Alimuddin Ali Besse Khalidatunnisa Rachmawaty Rachmawaty, Rachmawaty Yusminah Hala