Claim Missing Document
Check
Articles

Found 24 Documents
Search

PENGARUH VARIASI KONDISI FERMENTASI TERHADAP PRODUKSI METABOLIT ANTIBAKTERI EKSTRAK ISOLAT I5 FUNGI ENDOFIT Anredera cordifolia (Ten.) Steenis Ismail, Ismail; Megawati, Megawati; Ali, Alimuddin; Ningsih, Fuji Asmiati
Jurnal Ilmiah Manuntung Vol 5 No 2 (2019): Jurnal Ilmiah Manuntung
Publisher : jurnal ilmiah manuntung akademi farmasi samarinda

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (373.545 KB)

Abstract

Endophytic fungi are one of the microorganisms that live in plants and symbiotic with their host plant produce secondary metabolites. This study aims to determine the optimum growth profile of endophytic fungus isolated from the leaf of Anredera cordifolia (Ten.) Steenis namely isolate I5 as antibacterial. The fungus growth profile optimization analyzed by a correlation between fermentation time and mycelium weight, which collected every 24 hours for 15 days. The media were varied at pH conditions of 4, 7, and 8 in each PDYB medium (glucose and fructose substrates). The results showed that isolate I5 grew optimally on the 13th day on the PDYB medium pH 8. Ethyl acetate extract 5% of isolate I5 could inhibit bacteria growth with the largest inhibition zone diameter against Staphylococcus aureus of 9.96 mm (± 0.60) and Escherichia coli of 9.03 mm (± 0.46).
Purifikasi dan Karakterisasi Krom Reduktase Bacillus sp LKA9 Ali, Alimuddin; Soetarto, Endang Sutariningsih; Widada, J. Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (338.069 KB) | DOI: 10.24002/biota.v11i2.2625

Abstract

Chromate reductase is one of the potential enzymes for hexavalent chrom detoxification. Most of the enzyme is produced by bacteria, especially Bacillus. The aim of this research was to study chromate reductase activity isolated from Bacillus sp LKA9. Bacillus sp LKA9 was isolated from leather tannery liquid waste and used as a model in the experiment. Bacillus sp LKA9 was isolated through enrichment culture using Salt Base Solution containing 3 mM K2CrO4. Chromate reductase was isolated from bacteria by growing on a liquid medium containing chrom hexavalen (Cr VI) through several steps. The first step of the isolation process was to use the precipitated process using ammonium sulphate (30-80%). The next step, crude enzymes from the first step was partially purified through DEAE-Cellulose of Ion Exchange Chromatography Column. Diphenylcarbazide methods was used to examine the activity of enzyme fractions. The result of the experiment revealed that all protein could be precipitated by ammonium sulphate, and the cytoplasmic fraction at saturation of 50-70% showed high enzyme activity. Purified enzymes showed an increase activity 69,385 times to that of crude enzymes. The enzyme optimal had temperature and pH were 350C and 5; respectively. KM of enzyme was 0,0075 mM, and Vmax was 2500 mol/minute/mg protein. Enzyme activity was not inhibited by Cu2+, but an ion Ag2+ and Hg2+ inhibited the enzyme activity un-competitive. The activity of enzyme was demonstrated on SDS-PAGE by appearing typically band with molecular weight 29,26 kDa, it was assumed as chromate reductase.
PENINGKATAN AKTIVITAS, HASIL BELAJAR AFEKTIF DAN KOGNITIF PADA MATA PELAJARAN BIOLOGI MELALUI PENERAPAN MODEL BRAIN BASED LEARNING PESERTA DIDIK KELAS VII-A SMP NEGERI 4 SUNGGUMINASA KABUPATEN GOWA Hamid, Hamsinar; B., Nurhayati; Ali, Alimuddin
Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam Vol 3, No 1 (2014): Maret
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (643.947 KB) | DOI: 10.35580/sainsmat3110122014

Abstract

Penelitian ini bertujuan untuk mengetahui peningkatan aktivitas, hasil belajar afektif dan kognitif biologi peserta didik melalui penerapan model pembelajaran Brain Based Learning (BBL) pada materi ?Keseimbangan Ekosistem dan Pengelolaan Lingkungan? di kelas VIIA SMP Negeri 4 Sungguminasa Kabupaten Gowa. Penelitian ini merupakan penelitian tindakan kelas (Classroom Action Research). Penelitian dilaksanakan pada Semester Genap Tahun Pelajaran 2013/2014, pada bulan Desember 2014 yang bertempat di SMP Negeri 4 Sungguminasa Jl. Lapangan Syekh Yusuf No 03 Kabupaten Gowa. Instrumen yang digunakan dalam penelitian ini adalah lembar observasi aktivitas peserta didik, tes hasil belajar kognitif dan lembar hasil kegiatan afektif. Teknik pengumpulan data meliputi lembar observasi aktivitas peserta didik dan tes hasil belajar kognitif yang dianalisis secara kuantitatif dengan persentase. Hasil penelitian menunjukkan bahwa penerapan model pembelajaran Brain Based Learning dapat meningkatkan aktivitas belajar peserta didik dengan nilai rata-rata siklus I sebesar 48,74% dan pada siklus II meningkat menjadi 75,89%, meningkatkan hasil belajar kognitif peserta didik dengan presentase ketuntasan pada siklus I sebesar 52,78%, pada siklus II meningkat menjadi 94,44%. Demikian juga dengan hasil belajar afektif yang setiap indikatornya mengalami peningkatan dari siklus I ke siklus II.Kesimpulan dari hasil penelitian ini adalah penerapan model pembelajaran Brain Based Learning meningkatkan aktivitas, dan hasil belajar pada materi ?Keseimbangan Ekosistem dan Pengelolaan Lingkungan, peserta didik kelas VIIA SMP Negeri 4 Sungguminasa Kabupaten Gowa.Kata kunci: Model Brain Based Learning, Aktivitas Belajar, Hasil Belajar Afektif dan Kognitif.
DETEKSI ANTIBAKTERI PADA EKSTRAK DAUN MURBEI (MORUS ALBA L.) DARI BEBERAPA LOKASI PENGAMBILAN SAMPEL TANAMAN DI SULAWESI SELATAN Ali, Alimuddin; Kursia, Sukriani; Nadia, Nadia
bionature Vol 17, No 2 (2016): Oktober
Publisher : Fakultas MIPA UNM

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.224 KB) | DOI: 10.35580/bionature.v17i2.2833

Abstract

Murbei (Morus albaL.) merupakan salah satu jenis tanaman yang telah digunakan oleh masyarakat sebagai sumber bahan obat seperti antioksidan, antidiabetes, antihipertensi, hipolipidemik, demam, flu, batuk, antiemetik dan gangguan saluran pencernaan. Penelitian ini bertujuan untuk menentukan potensi aktivitas antibakteri daun murbei dari daerah Soppeng, Gowa dan Enrekang terhadap bakteri Escherichia coli. Daun murbei diekstraksi menggunakan larutan penyari etanol 70% dan etil asetat. Metode pengujian aktivitas antibakteri yang dilakukan adalah metode difusi agar dan analisis KLT bioautografi. Hasil penelitian menunjukkan bahwa ekstrak daun murbei asal kab.Soppeng, Gowa dan Enrekang memiliki potensi aktivitas antibakteri terhadap bakteri Escherichia coli dan yang memiliki potensi baik sebagai aktivitas antibakteri ialah ekstrak etanol dari daerah soppeng pada konsentrasi 10% dengan diameter zona hambatan yaitu 9,89 mm. Hasil pengujian analisis KLT bioautografi ekstrak etil asetat menunjukkan adanya aktivitas antibakteri dengan spot noda dari daerah Soppeng 5 noda, Gowa 6 noda dan Enrekang 6 noda sedangkan ekstrak etanol tidak menunjukkan adanya aktivitas antibakteri terhadap bakteri Escherichia coli. Kata kunci: Daun Murbei, Aktivitas Antibakteri, Difusi Agar, dan KLT Bioautografi.
Isolasi Senyawa Metabolit Sekunder Utama Ekstrak Etanol Kulit Batang Tumbuhan Mangrove (Avicennia spp.) Darminto, .; Ali, Alimuddin; Dini, Iwan
Sainsmat : Jurnal Ilmiah Ilmu Pengetahuan Alam Vol 1, No 1 (2012): Maret
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/sainsmat114602012

Abstract

DOWNLOAD PDFIn vitro examine of fractionated ethanol extract of bark Mangrove (Avicennia spp). showed high antibaterial activity. Repression test of extract formula showed that fish has a higher viability. Recent study has conducted to find the main compound as candidate prior to standardized product of phytopharmaca for future commercial purposes. The chromatography methods was  applied and  identification of  major compounds by analysis based on chemical test as well as spectral data of spectroscopic measurements. Study showed that main secondary metabolites in the form of alkaloid class of polar compounds. The white crystalline powder form characteristics has a melting point 172oC with molecular weight 232, and molecular formula is C12H12N2O3.   Key words: Main secondary metabolites, mangrove, Avicennia spp.      
Peningkatan Aktivitas Belajar dan Hasil Belajar Biologi Siswa melalui Strategi Pembelajaran Everyone Is a Teacher Here pada Kelas X SMA Negeri 3 Sengkang Amir, Suharyanti; Ali, Alimuddin; Azis, Andi Asmawati
Biology Teaching and Learning Vol 1, No 2 (2018): Desember
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/btl.v1i2.8179

Abstract

Abstract.  This research is a classroom action research. The purpose of this study improves the activity and student learning outcomes through application of learning strategy Everyone is a Teacher Here. Research subjects are students of class X.I SMA Negeri 3 Sengkang. This research carried out during two cycles. Data collection implemented in the second semester of academic year 2016/2017. This research measure learning activity and learning outcomes. Learning activity measure with observation sheet and learning outcomes measure with written test consist of multiple-choice question as much as 20 items. Student learning activities collected during the learning process observed by 3 observers using observation sheet, then learning outcomes data obtained through evaluation when teaching’s time first and second meeting was done and on third meeting was evaluation.  This matter occurs on each cycle. All the data obtained were analyzed through three ways: qualitative analysis for student learning activities, quantitative for student learning outcomes. Results of research on student learning activity that has been analyzed shows the average in the first cycle of 55.6%, while in the second cycle of 67.2%, so the difference in the increase of 11.6%. Student learning outcomes also increased where the average value on observation data of 69.6, 64.4 the first cycle, and the second cycle 74.8. It can be concluded that the application of learning strategy Everyone is a Teacher Here can increase the activity and student learning outcomes. Keywords: everyone is a teacher here learning strategy, learning activities, learning outcomes.
Profil Kemampuan Argumentasi Siswa SMA Pada Pembelajaran Biologi Bahri, Husnaini; Pallenari, Muhiddin; Ali, Alimuddin
Biology Teaching and Learning Vol 4, No 1 (2021): Juni
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/btl.v4i1.22072

Abstract

Abstract. The ability to argue is one of the 21st century skills that students need to have. The purpose of this study was to determine the scientific argumentation skills of students class XII at the Biringkanaya District High School in answering description questions, especially in the digestive system material. This type of research is a descriptive survey research using purposive sampling technique. The population of this study were students of class XII MIA at State Senior High Schools in Biringkanaya District, Makassar City, South Sulawesi. The sample used in this study is a study group in which students have heterogeneous cognitive abilities. The argumentation ability data is obtained through giving tests. Based on the test results, the overall argumentation skills of students were in the very poor category with a score of 19.74%. The results of the students' arguments showed that in the aspect of making claims with a percentage of 91.4% (very good); aspects include ground with a percentage of 21.64% (less); aspects include backing with a percentage of  9.88% (very low); aspects include a warranty with a percentage of 5.71% (very low); aspects include qualifier with a percentage of 5% (very low);  and aspects include rebuttals or reservations with a percentage of 7.3% (very low).Keywords: ability to argument, making claims, rebuttal, reservation, high schools Makassar 
Level of Practicality and Effectiveness of Practicum Guidelines in The Molecular Genetics Course for Students of Biology Undergraduate Study Programs Novira, Aulya; Jumadi, Oslan; Ali, Alimuddin; Hartono, Hartono
Biology Teaching and Learning Vol 6, No 2 (2023): Desember
Publisher : Universitas Negeri Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35580/btl.v6i2.54176

Abstract

Abstract. This type of research is Research and Development (R&D) which aims to determine the level of practicality, and effectiveness of guided inquiry-based molecular genetics guides. The research design uses the ADDIE development model, namely Analyze, Design, Develop, Implement, and Evaluate. The subjects of this research were Lecturers of Biology Department of Makassar State University, and Students. The instrument to test the validity uses a questionnaire sheet validated by an expert validator, to test the practicality of giving a student response questionnaire, lecturer response, and product implementation sheet, and test the effectiveness of multiple choice questions to test student learning outcomes. The data from this study showed that the development of this practicum guide was categorized as practical because the data obtained were in the interval 4 ≤ IO ≤ 5. The effectiveness test used T-Test analysis (Paired Sample Test). From the data analysis, it is obtained that the use of molecular genetics practicum guides is effective in increasing knowledge related to DNA isolation, PCR, and electrophoresis material in practicum activities after being given molecular genetics guides. It can be seen that the significance of p = 0.000 <0.01, meaning that the results obtained are very significant, it can be concluded that the developed guide shows effective on improving student cognitive learning outcomes.Keywords: practicum guide, molecular genetics, guided inquiry, practicality test, effectiveness test
Pengaruh Efikasi Diri, Kecerdasan Emosional, dan Motivasi Belajar Terhadap Hasil Belajar Biologi Peserta Didik SMA Negeri Kelas XI Se-Kecamatan Tanete Riattang, Kabupaten Bone: The Influence Of Self Efficacy, Emotional Intelligence, And Learning Motivation, On The Biology Learning Outcomes Of Class XI SMAN In The Tanete Riattang Sub District, Bone District Rahmi, Rahmi; Daud, Firdaus; Ali, Alimuddin
Anterior Jurnal Vol. 23 No. 3 (2024): Anterior Jurnal
Publisher : ​Institute for Research and Community Services Universitas Muhammadiyah Palangkaraya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33084/anterior.v23i3.7558

Abstract

Penelitian ini bertujuan untuk mengetahui pengaruh langsung dan tidak langsung efikasi diri dan kecerdasan emosional terhadap motivasi belajar biologi peserta didik kelas XI SMA Negeri se-Kecamatan Tanete Riattang Kabupaten Bone. Penelitian ini adalah penelitian kuantitatif jenis ex-post facto yang bersifat korelasional. Variabel dalam penelitian ini yaitu dua variabel bebas meliputi efikasi diri (X1), kecerdasan emosional (X2), satu variabel intervening yaitu motivasi belajar (Y) dan satu variabel terikat yaitu hasil belajar biologi (Z) peserta didik pada pembelajaran biologi. Populasi penelitian ini adalah seluruh peserta didik kelas XI SMA Negeri di Kecamatan Tanete Riattang, Kabupaten Bone, yang terdiri dari 4 sekolah. Sampel penelitian ini adalah peserta didik kelas XI SMA Negeri se-Kecamatan Tanete Riattang Kabupaten Bone. Pengambilan sampel penelitian ini yaitu Probability Sampling dengan teknik simple random sampling. Instrumen yang digunakan adalah kuesioner atau angket dan wawancara. Teknik analisis data yang digunakan yaitu teknik analisis statistik deskriptif, analisis statistik inferensial dan pengujian hipotesis. Efikasi diri dan kecerdasan emosional berpengaruh langsung terhadap motivasi belajar biologi peserta didik kelas XI SMA Negeri se-Kecamatan Tanete Riattang Kabupaten Bone. Efikasi diri, kecerdasan emosional dan motivasi belajar berpengaruh langsung terhadap hasil belajar biologi peserta didik kelas XI SMA Negeri se-Kecamatan Tanete Riattang Kabupaten Bone. Efikasi diri dan kecerdasan emosional berpengaruh tidak langsung terhadap hasil belajar biologi melalui motivasi belajar peserta didik kelas XI SMA Negeri se-Kecamatan Tanete Riattang Kabupaten Bone.
Peningkatan Kompetensi Guru MGMP Biologi SMA dan MA di Kabupaten Takalar Sulawesi Selatan Melalui Pelatihan Mikrobiologi Ali, Alimuddin; Hala, Yusminah; Muis, Abd; Palennari, Muhiddin; Rante, Herlina
Nanggroe: Jurnal Pengabdian Cendikia Vol 3, No 8 (2024): November
Publisher : Yayasan Daarul Huda Kruengmane

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

Program Pengabdian Masyarakat ini dilaksanakan pada bulan September 2024 di Laboratorium Sekolah SMA 1 Kabupaten Takalar, Sulawesi Selatan, dengan sasaran utama Guru MGMP Biologi dan IPA. Metode yang digunakan mencakup penyampaian teori, demonstrasi, latihan pembuatan media tumbuh, sterilisasi media, isolasi, serta diskusi dan sesi tanya jawab. Penyampaian teori bertujuan untuk memberikan pemahaman dasar mengenai praktikum Mikrobiologi. Demonstrasi dilakukan agar peserta dapat mengamati langsung prosedur dan tahapan sterilisasi serta pembuatan medium tumbuh menggunakan bahan dan alat yang murah dan mudah didapatkan disekitar sekolah. Praktik dimaksudkan agar peserta memperoleh keterampilan dalam melaksanakan proses kerja pembuatan media tumbuh mikroba. Diskusi dan sesi tanya jawab dirancang untuk mengatasi masalah yang muncul selama penyampaian teori dan kegiatan praktik. Berdasarkan kegiatan ini, dapat disimpulkan bahwa pelatihan ini berhasil meningkatkan pemahaman dan keterampilan guru dalam melaksanakan praktikum pembuatan media kultur mikroba menggunakan bahan dan peralatan sederhana dengan biaya rendah. Program ini membekali guru-guru dengan keterampilan untuk melaksanakan praktikum dengan mudah di laboratorium sekolah. Hasil pelatihan menunjukkan bahwa guru-guru peserta pelatihan mampu membuat media tumbuh mikroba serta memahami prinsip dasar pembuatan media dan isolasi mikroba. Kegiatan ini mampu meningkatkan minat dan pemahaman guru dalam merancang praktikum mikrobiologi menggunakan peralatan dan bahan yang ada di laboratorium sekolah dengan biaya yang sangat murah.