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Warda Amalia
State University of Medan
Education Languange, Linguistic, Communication & Media Other

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Analisis Penerapan Prinsip Bioetika dalam Pengelolaan Limbah B3 Cair Hasil Pemeriksaan Laboratorium Biologi di Balai Laboratorium Kesehatan Masyarakat Medan Warda Amalia; Valdo Rekson Nainggolan; Siti Nabila; Nurbaity Situmorang; Khairiza Lubis
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/e1sf2v72

Abstract

Kegiatan pemeriksaan di laboratorium biologi menghasilkan limbah yang berpotensi membahayakan kesehatan manusia dan lingkungan apabila tidak dikelola secara tepat. Limbah tersebut umumnya berupa limbah cair yang mengandung mikroorganisme patogen, bahan kimia, serta sisa media kultur yang termasuk dalam kategori limbah Bahan Berbahaya dan Beracun (B3). Penelitian ini bertujuan untuk mengidentifikasi jenis limbah B3 yang dihasilkan, mengetahui prosedur pengelolaan limbah, serta menganalisis penerapan prinsip bioetika dalam pengelolaan limbah di Balai Laboratorium Kesehatan Masyarakat Medan. Penelitian ini menggunakan metode deskriptif dengan pendekatan kualitatif melalui observasi dan wawancara. Hasil penelitian menunjukkan bahwa limbah yang dihasilkan terdiri dari limbah cair seperti sisa media kultur dan dan reagen. Limbah cair disterilisasi menggunakan autoclave sebelum dialirkan ke Instalasi Pengolahan Air Limbah (IPAL), sedangkan limbah padat disimpan sementara dan diangkut oleh transporter berizin. Prosedur ini mencerminkan penerapan prinsip bioetika, khususnya non-maleficence dan beneficence, untuk meminimalkan risiko terhadap kesehatan manusia
Analisis Cemaran Escherichia coli pada Sampel Makanan Menggunakan Metode Compact Dry Warda Amalia; Endang Sulistyarini Gultom Endang Sulistyarini Gultom
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/3kbf2x84

Abstract

Food safety is a critical aspect of protecting public health from foodborne diseases caused by microbiological contamination, with Escherichia coli serving as a key indicator of food hygiene and sanitation. This study aimed to analyze Escherichia coli contamination in food samples using the Compact Dry method and to evaluate its compliance with food safety standards. A descriptive method with a quantitative approach was employed on 20 food samples, which were tested using Compact Dry EC and incubated at 36 ± 0.5°C for 21 ± 2 hours. The results were expressed in CFU/g and compared with the standards set by SNI 7388:2009. The findings showed that 45% of the samples were positive for Escherichia coli, with contamination levels ranging from <1 to 1.25 × 10³ CFU/g, while 55% were negative. All positive samples did not meet food safety standards. Contamination was likely due to poor hygiene and sanitation practices, including cross-contamination during food processing and handling. The Compact Dry method proved to be effective, practical, and efficient for detecting Escherichia coli. These findings indicate that microbiological contamination in food remains a concern, highlighting the need to improve hygiene and sanitation practices to ensure food safety.
Co-Authors Endang Sulistyarini Gultom Endang Sulistyarini Gultom Khairiza Lubis Nurbaity Situmorang Siti Nabila Valdo Rekson Nainggolan