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Nurbaity Situmorang
State University of Medan
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Penerapan Prinsip Bioetik dalam SOP Pengendalian Hama Oryctes rhinoceros pada Perkebunan Kelapa Sawit Celine Sitinjak; Farina Justin Sihombing; Nurbaity Situmorang; Mhd Yusuf Nasution
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/x2jxzw46

Abstract

Kelapa sawit merupakan salah satu komoditas perkebunan yang memiliki peranan penting dalam perekonomian Indonesia. Namun, produktivitas tanaman kelapa sawit sering terganggu oleh serangan hama, salah satunya adalah Oryctes rhinoceros yang dikenal sebagai kumbang tanduk. Penelitian ini bertujuan untuk menganalisis penerapan prinsip bioetik dalam Standar Operasional Prosedur (SOP) pengendalian hama Oryctes rhinoceros pada perkebunan kelapa sawit di PT. Socfin Indonesia Kebun Bangun Bandar. Penelitian ini menggunakan pendekatan kualitatif dengan metode observasi langsung dan wawancara terhadap pekerja di lapangan. Hasil penelitian menunjukkan bahwa penerapan SOP pengendalian hama telah dilaksanakan dengan baik melalui penggunaan alat pelindung diri serta prosedur penyemprotan yang terstruktur. Penerapan tersebut mencerminkan prinsip bioetik terutama dalam aspek keselamatan kerja dan tanggung jawab terhadap lingkungan. Namun demikian, pemahaman pekerja terhadap konsep bioetik masih terbatas karena sebagian besar pekerja hanya memahami SOP sebagai aturan kerja. Oleh karena itu, diperlukan peningkatan sosialisasi dan pelatihan terkait bioetik agar penerapan SOP dapat dilakukan secara lebih optimal dan berkelanjutan.
Analisis Penerapan Prinsip Bioetika dalam Pengelolaan Limbah B3 Cair Hasil Pemeriksaan Laboratorium Biologi di Balai Laboratorium Kesehatan Masyarakat Medan Warda Amalia; Valdo Rekson Nainggolan; Siti Nabila; Nurbaity Situmorang; Khairiza Lubis
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/e1sf2v72

Abstract

Kegiatan pemeriksaan di laboratorium biologi menghasilkan limbah yang berpotensi membahayakan kesehatan manusia dan lingkungan apabila tidak dikelola secara tepat. Limbah tersebut umumnya berupa limbah cair yang mengandung mikroorganisme patogen, bahan kimia, serta sisa media kultur yang termasuk dalam kategori limbah Bahan Berbahaya dan Beracun (B3). Penelitian ini bertujuan untuk mengidentifikasi jenis limbah B3 yang dihasilkan, mengetahui prosedur pengelolaan limbah, serta menganalisis penerapan prinsip bioetika dalam pengelolaan limbah di Balai Laboratorium Kesehatan Masyarakat Medan. Penelitian ini menggunakan metode deskriptif dengan pendekatan kualitatif melalui observasi dan wawancara. Hasil penelitian menunjukkan bahwa limbah yang dihasilkan terdiri dari limbah cair seperti sisa media kultur dan dan reagen. Limbah cair disterilisasi menggunakan autoclave sebelum dialirkan ke Instalasi Pengolahan Air Limbah (IPAL), sedangkan limbah padat disimpan sementara dan diangkut oleh transporter berizin. Prosedur ini mencerminkan penerapan prinsip bioetika, khususnya non-maleficence dan beneficence, untuk meminimalkan risiko terhadap kesehatan manusia
Kode Etik Pengelolaan Limbah Bekas Dan Bahan Terkontaminasi Pada Laboratorium Kultur Jaringan Tanaman Najwa Athifah Athifah; Ribi Zuhriah Sani Sani; Mhd Yusuf Nasution; Khairiza Lubis; Nurbaity Situmorang
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/rabwff67

Abstract

This study aims to describe the management of used culture media waste and contaminated materials, and to assess the understanding of laboratory staff and interns regarding ethical principles in plant tissue culture laboratories. A descriptive qualitative method was applied using observation, questionnaires, and literature review. The results indicate that most respondents have a good understanding, reflected in their agreement on the importance of sterilization, waste segregation, use of personal protective equipment, and implementation of standard operating procedures (SOP). Respondents are also aware of the risks of laboratory waste to health and the environment. Ethical practices are recognized as part of professional responsibility, but require improvement through training, supervision, and consistent SOP implementation.  
ANALISIS PENERAPAN PRINSIP BIOETIK PADA PENGELOLAAN LIMBAH B3 DILABORATORIUM KESEHATAN DAERAH KOTA BEKAS NAUFAL MAHENDRA; ELSHA NURISA SIDABUTAR; Nurbaity Situmorang; Khairiza Lubis
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/m7ztf382

Abstract

The management of hazardous and toxic waste (B3) in health laboratories remains a challenge, particularly inensuring regulatory compliance while consistently applying bioethical principles. This issue is important becauseimproper waste management can negatively impact the environment and public health. Therefore, this study aimsto analyze the B3 waste management system, its compliance with regulations, and the implementation of bioethicalprinciples at the Regional Health Laboratory (Labkesda) of Bekasi City. This study employed a descriptivequalitative method with a case study approach. Data were collected through interviews, observations, anddocumentation involving two informants directly engaged in waste management. In addition, the implementationof bioethics was assessed using a Likert scale with ten indicators. The results show that B3 waste management hasbeen carried out in a structured manner, including identification, containment, transportation, temporary storage,and processing by licensed third parties, thus complying with applicable regulations. Furthermore, theimplementation of bioethical principles achieved an average score of 4.8 out of 5, indicating a very good category.However, an obstacle was identified in the form of delays in waste collection by third parties. In conclusion,Labkesda Bekasi City has been able to manage B3 waste effectively and sustainably, although improvements inexternal coordination are still needed
Penerapan Prinsip Bioetik dalam Pengujian Escherichia coli pada Air dan Salmonella pada Ikan Nila (Oreochromis niloticus) Menggunakan Media Compact Dry Berdasarkan Standar ISO/IEC 17025 di Laboratorium BPPMHKP Medan I Andina Ayu Syahputri; Dini Dara Arifia; Nurbaity Situmorang; Mhd Yusuf Nasution; Widya Arwita
Jurnal Biogenerasi Vol. 11 No. 2 (2026): April - Juni 2026
Publisher : Universitas Cokroaminoto Palopo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30605/xk28gj62

Abstract

Food safety and environmental quality are critical aspects in maintaining public health, particularly in fishery products and water sources that are vulnerable to contamination by pathogenic microorganisms. This study aims to identify the presence of Escherichia coli in water samples and Salmonella in tilapia (Oreochromis niloticus), as well as to analyze the use of Compact Dry media based on ISO/IEC 17025 and the implementation of bioethical principles in microbiological testing. This research employed a descriptive laboratory approach conducted at the BPPMHKP Medan I Laboratory using Compact Dry media based on AOAC methods. The samples consisted of lake water and tilapia, which were tested through enrichment, inoculation, incubation, and colony observation stages. The results showed no growth of Escherichia coli colonies on Compact Dry EC media and no Salmonella colonies on Compact Dry SL media, indicating that all samples were negative for the tested bacteria. The use of Compact Dry media in this study complied with ISO/IEC 17025 standards, as indicated by systematic procedures, the use of validated media, and laboratory quality control. Furthermore, bioethical principles, including scientific integrity, responsibility, biosafety, and accountability, were applied throughout all stages of testing. Therefore, this study demonstrates that the use of validated media and the application of bioethical principles play an important role in ensuring valid, reliable, and accountable laboratory results.
Co-Authors Andina Ayu Syahputri Celine Sitinjak Dini Dara Arifia ELSHA NURISA SIDABUTAR Farina Justin Sihombing Khairiza Lubis Mhd Yusuf Nasution Najwa Athifah Athifah NAUFAL MAHENDRA Ribi Zuhriah Sani Sani Siti Nabila Valdo Rekson Nainggolan Warda Amalia Widya Arwita