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IDENTIFIKASI ISOLAT KULIT BATANG WARU (Hibiscus tiliaceus L.) MENGGUNAKAN SPEKTROSKOPI INFRAMERAH Taupik, M.Sc, Muhammad; Adam Mustapa, Muhammad
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

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Waru (Hibiscustiliaceus L.) bark is a plant that is believed and used by community as a traditional medicine in treatment, especiallytotreat fever. This study aims to analyze the level of a secondary metabolite compound in the bark of waru (Hibiscustiliaceus L.) plant calculated using UV-Vis spectrophotometry and infrared methods. The method used to identify the flavonoid content is Thin Layer Chromatography (TLC) using eluent n-hexane and ethyl acetate at the best comparison (7:3). The result obtained from this study is waru bark extract containing flavonoid compound which is shown from the Rf value of the waru bark methanol extract of 0.82, which the value is close to the Rf value of quarcetine 0.83. Analysis of the flavonoid content of waru bark methanol extract has carried out on The Spectrophotometric UV-Vis at a wavelength of 382 nm and the total flavonoid content obtained is of 10 mg of waru bark methanol extract containing135.2166µg/mL flavonoid compound with percentage of 13.521% and on the Spetroscopy Infrared, based on the obtained peak shows the existence of functional groups of OH, CH aliphatic, C=C aromatic and C-O indicates that this isolat is a flavonoid compound.

ANALISIS KADAR FLAVANOID TOTAL MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS DALAM KULIT BUAH SALAK (Salacca zalacca V.) Adam Mustapa,M.Sc, Mohammad; Taupik, Muhammad; Ramadhan L, Aditya
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

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The Snake Fruit (Salacca zalacca (Gaertn.) Voss) skin is considered as a waste, but there are a small number of people who use this fruit skin as an medicine. A compound that plays a role in snake fruit skin for the treatment is flavonoid. The purpose of this study was to determine the level of flavonoid contained in the extract of snake fruit skin. The method used to determine the amount of flavonoid compound contained in it is the UV-Vis Spectrophotometry method. The result of reading the absorbance value of each snake fruit fraction obtained from the methanol fraction was 0.568 and ethyl acetate fraction of 0.319, which were then calculated by using the quercetin linear equation standard, y = 0.0608 x -0.0188 with the correlation coefficient (R2) = 0.997. The result obtained for each snake fruit fraction for flavonoid level in the methanol fraction was 96.51 µg/mL and in ethyl acetate fraction was 55.56 µg/mL.

ANALISIS KADAR FLAVANOID TOTAL MENGGUNAKAN SPEKTROFOTOMETRI UV-VIS DALAM KULIT BUAH SALAK (Salacca zalacca V.) Adam Mustapa,M.Sc, Mohammad; Taupik, Muhammad; Ramadhan L, Aditya
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (547.013 KB)

The Snake Fruit (Salacca zalacca (Gaertn.) Voss) skin is considered as a waste, but there are a small number of people who use this fruit skin as an medicine. A compound that plays a role in snake fruit skin for the treatment is flavonoid. The purpose of this study was to determine the level of flavonoid contained in the extract of snake fruit skin. The method used to determine the amount of flavonoid compound contained in it is the UV-Vis Spectrophotometry method. The result of reading the absorbance value of each snake fruit fraction obtained from the methanol fraction was 0.568 and ethyl acetate fraction of 0.319, which were then calculated by using the quercetin linear equation standard, y = 0.0608 x -0.0188 with the correlation coefficient (R2) = 0.997. The result obtained for each snake fruit fraction for flavonoid level in the methanol fraction was 96.51 g/mL and in ethyl acetate fraction was 55.56 g/mL.

IDENTIFIKASI ISOLAT KULIT BATANG WARU (Hibiscus tiliaceus L.) MENGGUNAKAN SPEKTROSKOPI INFRAMERAH Taupik, M.Sc, Muhammad; Adam Mustapa, Muhammad
Journal Syifa Sciences and Clinical Research Vol 1, No 1 (2019): March
Publisher : State University of Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (663.495 KB)

Waru (Hibiscustiliaceus L.) bark is a plant that is believed and used by community as a traditional medicine in treatment, especiallytotreat fever. This study aims to analyze the level of a secondary metabolite compound in the bark of waru (Hibiscustiliaceus L.) plant calculated using UV-Vis spectrophotometry and infrared methods. The method used to identify the flavonoid content is Thin Layer Chromatography (TLC) using eluent n-hexane and ethyl acetate at the best comparison (7:3). The result obtained from this study is waru bark extract containing flavonoid compound which is shown from the Rf value of the waru bark methanol extract of 0.82, which the value is close to the Rf value of quarcetine 0.83. Analysis of the flavonoid content of waru bark methanol extract has carried out on The Spectrophotometric UV-Vis at a wavelength of 382 nm and the total flavonoid content obtained is of 10 mg of waru bark methanol extract containing135.2166g/mL flavonoid compound with percentage of 13.521% and on the Spetroscopy Infrared, based on the obtained peak shows the existence of functional groups of OH, CH aliphatic, C=C aromatic and C-O indicates that this isolat is a flavonoid compound.

Kajian Fitokimia dan Identifikasi Senyawa Metaboli Sekunder Daun Pare (Momordica Charantia L.) Muhammad Taupik; Endah Nurrohwinta Djuwarno; Moh Adam Mustapa
Al-Kimia Vol 9 No 2 (2021): DESEMBER
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/al-kimia.v9i2.23633

Pare plant is a plant that is found in almost every region in the country Indonesia. Plant is used as a traditional medicine to eliminate some diseases. The aim of this study was to isolate and identify the compounds terpenoids methanol extract fraction of n-hexane pare leaf (Momordica charantia L.) using UV-VIS spectrophotometry. Insulation done by maceration method using methanol to produce condensed methanol extract as much as 36.25 grams. Extracts condensed methanol produced is tested phytochemical. Phytochemical test results shows that it contains alkaloids, saponins and steroid / terpenoids. Condensed methanol extract then fractionated using liquid-liquid partition with solvent n-hexane and methanol with a ratio of 4: 2 ie 133 ml of n-hexane and 67 mL of methanol. Non-polar phase (phase n-hexane) results of further partitioning is evaporated using a rotary evaporator to produce a thick n-hexane extract as much as 1.25 grams hereinafter in the TLC test ratio of solvent to produce the best. Solvents are chosen that n-hexane: ethyl acetate (3: 1). Separation and purification was performed using Vacuum Liquid Chromatography (KCV) using silica gel GF254 as stationary phase and solvent n-hexane: methanol as mobile phase and eluted gradually. Isolates were then tested using Thin Layer Chromatography (TLC). Isolates were identified using UV-VIS spectrophotometry. Analysis of physicochemical data qualitatively using UV-VIS spectrophotometry on samples of the methanol extract of N-Hexane fraction pare leaf (Momordica charantia L.) class of compounds containing triterpenoids. This is because the absorption band at a wavelength of 274.2 nm and 432.8 nm with absorbance respectively 0.601 and 0.177 were suspected because of the transition of an electron from n à π*.

The Type Fragmentation Patterns Confirmed Acetaminophen By Using Liquid Chromatography-Mass Spectroscopy (LCMS) From Herbal Medicine (Jamu) Muhammad Taupik; Endah Nurrohwinta Djuwarno; Moh Adam Mustapa; Wiwin R Kunusa; Jafar La Kilo; Muhamad Handoyo Sahumena
Elkawnie: Journal of Islamic Science and Technology Vol 7, No 2 (2021)
Publisher : Universitas Islam Negeri Ar-Raniry Banda Aceh

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22373/ekw.v7i2.7492

Abstract: The Jamu or herbal medicine were adulterated with chemical drugs in the last twenty years. One of the chemical drugs that are commonly added to herbal medicine is acetaminophen. Acetaminophen is generally considered an analgesic-antipyretic drug. The research aims to identify the content of paracetamol and its amount added to the jamu. This study consisted of 6 jamu or herbal medicine samples with different brands. The TLC method conducted the qualitative analysis using the mobile phase n-hexane: ethyl acetate (1:1). Three positive samples containing acetaminophen were obtained: D, E, and F. The three positive samples containing acetaminophen were calculated using the LCMS method. Quantitative analysis was conducted with LCMS using a reversed-phase with a mobile phase of acetonitrile: water (15%>: 85%> v/v) at a flow rate of 0.2 mL/min and an injection volume of 5 µL, it was obtained the levels of acetaminophen in sample D as much as 0.16 g/2 g of sample, in sample E of 0.63 g/7 g of sample, and sample F of 0.56 g/7 g of sample. Although the acetaminophen content in herbal medicine is relatively small, according to the Regulation of the Minister of Health of Indonesia No. 007 of 2012, traditional medicine or herbal medicine should not contain synthetic chemicals.Abstrak: Dalam dua puluh tahun terakhir, banyak jamu yang dipalsukan dengan obat-obatan kimia. Salah satu obat kimia yang biasa ditambahkan pada jamu adalah asetaminofen. Acetaminophen umumnya dianggap sebagai obat analgesik-antipiretik. Penelitian ini bertujuan untuk mengetahui kandungan parasetamol dan jumlah yang ditambahkan pada jamu. Sampel penelitian ini terdiri dari 6 sampel jamu dengan merek yang berbeda. Analisis kualitatif dilakukan dengan metode KLT menggunakan fase gerak n-heksana: etil asetat (1:1). Diperoleh tiga sampel positif mengandung asetaminofen, yaitu sampel D, E, dan F. Tiga sampel positif mengandung asetaminofen dihitung menggunakan metode LCMS. Analisis kuantitatif dilakukan dengan LCMS menggunakan fase terbalik dengan fase gerak asetonitril: air (15%>:85%> v/v) pada laju alir 0,2 mL/menit dan volume injeksi 5 µL. Diperoleh kadar asetaminofen pada sampel D sebanyak 0,16 g/2 g sampel, pada sampel E sebesar 0,63 g/7 g sampel, dan pada sampel F sebesar 0,56 g/7 g sampel. Meskipun kandungan asetaminofen dalam jamu relatif sedikit, namun menurut Peraturan Menteri Kesehatan RI No. 007 Tahun 2012, obat tradisional atau jamu tidak boleh mengandung bahan kimia sintetik.

UJI AKTIVITAS ANTIBAKTERI EKSTRAK METANOL DAUN KECUBUNG (Datura metel L.) TERHADAP BAKTERI Streptococcus pneumonia DAN Klabsiella pneumonia A. Mu’thi Andy Suryadi; Moh Adam Mustapa; Faramita Hiola; Sintiya Basiru
Indonesian Journal of Pharmaceutical Education Vol 1, No 3 (2021): September-Desember 2021
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v1i3.11776

Infectious disease is a disease caused by microbes, including bacteria. One of the microorganisms that often causes infectious disease is Streptococcus pneumoniae and Klabsiella pneumoniae. Based on empirical data, plant that has antimicrobial potential is amethyst leaves (Datura metel L.). This study aims to know the antibacterial activity and concertration of amethyst leaves (Datura metel L.) against Streptococcus pneumoniae and Klabsiella pneumonia. This is an experimental study which includes antibacterial activity test, MIC (Minimum Inhibitor Concertration) test, MFC (Minimum Fungicidal Concertration) test, and bacterial potency test. The finding shows that the antibacterial activity test of amethyst leaves (Datura metel L.) methanol extract is able to inhibit bacterial growth of Streptococcus pneumonia at a minimum inhibitor concertration of 15% and an optimum concertration of 50% with an average of 16.33 mm and 19.30 mm. Meanwhile, for Klabsiella pneumoniae, the minimum inhibitor concertration is 20% and the optimum concertration is 50%, with an average of 13.82 mm and 17.73 mm. this is based on the results of One Way Anova data (a 0.01) with a 99% confidance level.

Identifikasi Senyawa Alkaloid Pada Daun Bidara Arab (Ziziphus spina-christi L.) Menggunakan Metode Spektrofotometri UV-Vis A. Mu’thi Andy Suryadi; Mohammad Adam Mustapa; Nur Khofifah Zahrah
Indonesian Journal of Pharmaceutical Education Vol 2, No 1 (2022): Januari-April 2022
Publisher : Jurusan Farmasi Universitas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37311/ijpe.v2i1.11687

Christ's torn jujube (Ziziphus spina-christi L.) is a medicinal plant that has many benefits for curing or treating diarrhea, fever, and cancer. One of the compounds that play role is an alkaloid. This study was generated to identify the alkaloid compounds in the leaves of the Christ’s jujube torn using the UV-Vis spectrophotometry method. Christ's torn jujube leaves were macerated using 3 types of solvents with different polarity levels i.e., n-hexane, ethyl acetate, and methanol. Furthermore, phytochemical screening of alkaloid compounds was carried out. A Christ jujube leaf sample that was positive for alkaloids was found in the ethyl acetate extract; then identified using thin layer chromatography with eluent n-hexane: ethyl acetate (8:2), yielding 4 points with an Rf value of 0.25; 0.4 ; 0.52 ; and 0.62. Furthermore, phytochemical screening of alkaloid compounds was carried out. Christ' torn jujube which were positive for alkaloids present in the ethyl acetate extract were identified using thin layer chromatography with n-hexane eluent: ethyl acetate (8:2), yielding four spots with an Rf value of 0.25; 0.4 ; 0.52 ; and 0.62. The four spots were identified using UV-Vis spectrophotometry, obtained a wavelength of 248 ; 268 ; 268 ; and 255. Based on the identification results on the leaf extract, each stain was suspected to contain cantin-6-one alkaloid compounds.

ISOLASI SENYAWA ALKALOID DARI TUMBUHAN MAHONI (Switenia mahagoni Jacq) Muhammad Taupik; Endah Nurrohwinta Djuwarno; Moh Adam Mustapa
Pharmasipha: Pharmaceutical Journal of Islamic Pharmacy Vol 5, No 2 (2021): September
Publisher : University Of Darussalam Gontor

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21111/pharmasipha.v5i2.6663

Penelitian ini bertujuan untuk mengidentifikasi senyawa Alkaloid yang terkandung didalam biji buah mahoni (Switenia mahagoni Jacq). Senyawa target hasil isolasi diidentifikasi menggunakan spektrofotometri UV-Vis. Proses pengambilan senyawa menggunakan metode refluks hingga diperoleh ekstrak kental berwarna hijau gelap sebanyak 27 gram. Identifikasi awal dilakukan dengan skrining fitokimia pada ekstrak kental methanol, meliputi skrining senyawa alakaloid, flavonoid, dan saponin. Proses fraksinasi dilakukan dengan sistem partisis cair-cair menggunakan n-heksanaa dan metanol dan selanjutnya hasil partisi dipekatkan dengan rotary evaporator. Fraksi metanol dielusi pada plat KLT (Kromatografi Lapis Tipis) guna mendapatkan formulasi eluen terbaik untuk dilanjutkan pada Kromatografi Cair Vakum (KCV) dan diperoleh formula eluen terbaik n-heksanaa:metanol (4:1). Proses pemisahan menggunakan KCV dengan silica gel 60 PF254, dengan system eluen n-heksana:metanol bergradien. Isolat dievaluasi kemurniannya menggunakan KLT. Isolat diidentifikasi menggunakan Spektrofotometri UV-Vis dan serapan diperoleh pada panjang gelombang gelombang 208, 240, 286 nm. Nilai absorbansi berturutturut 0,318; 0,852; dan 0,443 A. Hasil interpretasi data mengkonfirmasi bahwa terdapat ikatan rangkap terkonjugasi dikarenakan serapan terbentuk pada panjang gelombang 250-800 nm. Diduga terjadi transisi nπ* yang mengindikasikan gugus N-H dengan abrosbansi pada rentang ultraviolet kuarsa (200-400 nm).

Peningkatan Potensi Ekonomi Lokal Melalui Teknologi Pengembangan Produk Minyak Balur Dari Kelapa Moh Adam Mustapa; Endah Nurrhohwinta Djuwarno
Jurnal Sibermas (Sinergi Pemberdayaan Masyarakat) Vol 8, No 3 (2019): Jurnal Sbermas (Sinergi Bersama Masyarakat)
Publisher : Univeristas Negeri Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.37905/sibermas.v8i3.7983

Potensi Kelapa di Desa Didingga sangat berlimpah, untuk pengelolaannya saat ini belum menjadi perhatian masyarakat dalam pemanfaatan Kelapa, saat ini masih kurang Produktif, karena hasil kelapa yang sangat banyak maka perlu adanya teknologi dalam pengembangan kelapa menjadi minyak balur serta produk farmasi dan kosmetik. Untuk dapat meningkatkan nilai jual kelapa minyak balur dimasa pandemic covid 19 perlu adanya teknologi pengembangan kelapa minyak balur dan dilakukan penganekaragaman pengelohan produk yang bernilai tinggi,misalnya minyak gosok, aromaterapi dan bedak lulur. selain itu produk tersebut diedarkan keswalayan, Toko dan Apotik di Kota dan kabupaten Gorontalo Utara. Produk-produk tersebut sangat bermanfaat bagi masyarakat contonya Minyak Balur untuk pengelinu, patah tulang dan antiseranga, Aromaterapi khasiatnya untuk masuk angin, Bedak Lulur untuk perawatan kulit dan kosmetik lainya . Permasalahan berantai yang sangat menganjal adalah keterbatasan teknologi yang bisa diakses dalam hal teknologi pembuatan alat pembuatan minyak balur dan penampungannya, alat dan bahan dalam pembuatan produk kelapa serta pengemasan. Ini semua sangat mempengaruhi ekonomi lokal masyarakat. Aspek inilah yang menjadi permasalahan mendasar oleh kelompok masyarakat tentang teknologi pengembangan dan peningkatan potensi ekonomi lokal dalam hal mengembangkan Kelapa Bentuk program yang akan dilaksanakan oleh peserta KKN Tematik adalah program melestarikan desa Didingga, program pengolahan produk, program pengemasan, serta program pemasaran, program tersebut akan didistribusikan mahasiswa sesuai kebutuhan. Metode yang digunakan dalam melakukan pemberdayaan pendampingan kelompok sasaran adalah teknik pembelajaran kelompok disertai praktek yang akan nantinya bersinergi untuk mengembangkan produk farmasi dan kosmetik yang sudah terkemas dengan baik sesuai standart mutu dan kesukaan masyarakat nanti sehingga dihasilkan produk yang berkualitas dan memiliki daya saing serta pemasaran secara aktif untuk memperkenalkan lebih luas kemasyarakat dan didapatkan potensi pangsa pasar secara berkelanjutan pada program kelompok Petani dan PKK nantinya.

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