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All Journal Equilibrium Journal of Chemical Engineering
Lestari, Maghfira Arum
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Chemical Engineering, Chemistry & Bioengineering Chemistry Energy Engineering Materials Science & Nanotechnology

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ANALYSIS OF AMMONIA ASSIMILATION KINETICS AND DETERMINATION OF SUBSTRATE INHIBITION CONDITIONS IN VARIATIONS OF MOLASSES DOSE IN A BATCH REACTOR SYSTEM Putra, Muhammad Iqbal; Arvianto, Rizky Ibnufaatih; Suhirman, Suhirman; Ardian, Adna Ivan; Puteri, Denanda Clarasati; Nury, Dennis Farina; Luthfi, Muhammad Zulfikar; Lestari, Maghfira Arum
Equilibrium Journal of Chemical Engineering Vol 10, No 1 (2026): Volume 10, No 1 July 2026 (First Online)
Publisher : Program studi Teknik Kimia UNS

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20961/equilibrium.v10i1.115734

Abstract

The purpose of this study is to find the inhibitory phenomena caused by an excessive organic load and to estimate the ideal molasses dosage for ammonia assimilation by Saccharomyces spt. The experiment was carried out utilizing a batch reactor system with different molasses doses of 0, 5, 10, 15, and 20 mL at a constant starting ammonia content of 50 mg/L. Ammonia assimilation followed a pseudo-first-order model with a significant coefficient of determination R2 > 0.90 in the active dose range, according to the kinetic studies. The results confirmed that a dose of 10 mL was the optimum condition, producing the highest reaction rate constant (k) of 0.5107 day-1 and an ammonia reduction efficiency of 93.58%. On the other hand, raising dosage 20 mL caused a substrate inhibition phenomenon, which was marked by a drop in the k value to 0.2268 day-1 and a low ammonia reduction efficiency of 58.42% because of the initial acidification. The ammonia removal rate and biomass concentration (MLSS) had a very strong positive linear connection (r=0.98) according to Pearson correlation analysis, indicating that nitrogen assimilation with biomass growth-rather than physical volatilization-is the primary mechanism of removal. In order to optimize ammonia absorption performance without causing environmental toxicity, this study suggests a dose of 10 mL.
Co-Authors Ardian, Adna Ivan Arvianto, Rizky Ibnufaatih Dennis Farina Nury Muhammad Zulfikar Luthfi Puteri, Denanda Clarasati Putra, Muhammad Iqbal SUHIRMAN SUHIRMAN