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All Journal BioEksakta : Jurnal Ilmiah Biologi Unsoed Agroradix : Jurnal Ilmu Pertanian
Mohsin Rafique
Unknown Affiliation
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Veterinary

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Identification and Characterization of A Fungal Isolates Associated with Anthracnose Symptoms on Avocado (Persea americana Mill.) from West Sumatra Anisa Rahman Rusiati; Riska Riska; Feskaharny Alamsjah; Mohsin Rafique; Ayumi Rizci Puspita
AGRORADIX : Jurnal Ilmu Pertanian Vol. 9 No. 2 (2026): Januari-Juli 2026
Publisher : Agroteknologi Fakultas Pertanian Universitas Islam Darul 'Ulum (UNISDA)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.52166/agroteknologi.v9i2.13235

Abstract

Anthracnose is one of the major postharvest diseases affecting avocado (Persea americana Mill.) and is commonly associated with fungi from the genus Colletotrichum. However, other fungal genera may also be associated with anthracnose-like symptoms in avocado fruits. This study aimed to identify fungal isolates associated with anthracnose symptoms on avocado fruits collected from West Sumatra using morphological and molecular approaches. Symptomatic fruits were incubated to induce lesion development, followed by fungal isolation and purification on Potato Dextrose Agar (PDA). Morphological characterization included macroscopic and microscopic observations. Molecular identification was performed using LSU rDNA amplification, sequencing, BLAST analysis, and phylogenetic reconstruction. A single fungal isolate, DTTDL318, was successfully obtained from avocado fruit showing anthracnose symptoms. Morphologically, the isolate exhibited characteristics consistent with the genus Fusarium, including white cottony colonies with orange reverse pigmentation, septate hyphae, fusiform conidia with pointed ends, and thick-walled chlamydospores. LSU sequence analysis indicated that the isolate belongs to the Fusarium solani species complex (FSSC) Phylogenetic analysis demonstrated that isolate DTTDL318 clustered in the same clade with Fusarium cf. solani and Fusarium waltergamsii with a bootstrap value of 88%, indicating a close phylogenetic relationship with members of the Fusarium solani species complex (FSSC). These findings indicate the presence of a Fusarium isolate associated with anthracnose-like lesions on avocado fruits.
Skrining Jamur dari Sonneratia caseolaris di Mangrove Mandeh: Sumber Potensial Kandidat Antioksidan Anthoni Agustien; Mohsin Rafique; Mildawati Mildawati; Rita Maliza; Mifthahul Jannah
BioEksakta : Jurnal Ilmiah Biologi Unsoed Vol 7 No 4 (2025): BioEksakta
Publisher : Fakultas Biologi Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.bioe.2025.7.4.17933

Abstract

Jamur endofitik yang berasosiasi dengan mangrove Sonneratia sp. diketahui menghasilkan metabolit sekunder yang berpotensi sebagai antioksidan. Kawasan pesisir Mandeh merupakan habitat alami yang kaya akan Sonneratia sp., sehingga menjadi lokasi strategis untuk eksplorasi jamur endofitik.Tujuan dari penelitian ini adalah untuk mengeksplorasi jamur endofitik yang berasosiasi dengan mangrove Sonneratia sp., melakukan skrining aktivitas antioksidan secara in vitro, serta mengidentifikasi isolat potensial berdasarkan karakteristik makroskopis dan mikroskopis.Penelitian ini menggunakan metode eksperimen dengan tahapan sebagai berikut: isolasi jamur dilakukan melalui teknik direct planting, skrining aktivitas antioksidan dilakukan secara in vitro menggunakan metode DPPH, dan identifikasi isolat dilakukan berdasarkan karakteristik morfologi makroskopis dan mikroskopis. Hasil penelitian menunjukkan bahwa sebanyak 20 isolat jamur endofitik berhasil diperoleh. Berdasarkan hasil skrining aktivitas antioksidan, ditemukan empat isolat yang menunjukkan aktivitas antioksidan dengan kategori kuat, sedang, dan lemah. Isolat dengan kode T4L1C4 dan T3L2C3 menunjukkan aktivitas antioksidan kuat, sedangkan T3L2C2 menunjukkan aktivitas sedang. Berdasarkan karakteristik mikroskopis, ketiga isolat tersebut teridentifikasi sebagai anggota genus Fusarium sp. Kata kunci: Antioksidan; Endofitik; Jamur; Mandeh; Skrining
Co-Authors Anisa Rahman Rusiati Anthoni Agustien Ayumi Rizci Puspita Feskaharny Alamsjah Maliza, Rita Mifthahul Jannah Mildawati Mildawati Riska Riska