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Antibacterial potency of fresh extract leaves of jamaican cherry (Muntingia calabura L.) in Inhibiting the growth of Shigella dysenteriae. Ikel Fitri Yanis; Feskaharny Alamsjah; Anthoni Agustien; Tesri Maideliza
Jurnal Biologi Universitas Andalas Vol 8, No 1 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.8.1.14-19.2020

Abstract

Research on the antibacterial potency of fresh extract from leaves of Jamaican cherry (Muntingia calabura L.) in inhibiting the growth of Shigella dysenteriae had been conducted in the Microbiology Laboratory, Department of Biology, Faculty of Mathematics and Natural Sciences, Andalas University. The study aimed to determine the potential of fresh extract from  Jamaican cherry leaves in inhibiting the growth of S. dysenteriae and to determine its Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) against S. dysenteriae. The results showed that the fresh extract of Jamaican cherry leaves was potent as an antimicrobial agent against pathogenic bacteria S. dysenteriae. It was shown through 12 - 14 mm diameter of inhibition zone which was classified as strong inhibition. The MIC was measured at 3.125% while MBC was undetermined. According to these findings, it can be concluded that the fresh extract from Jamaican cherry leaves was potent to inhibit the growth of S.dysentriae at 3.125% concentration, yet unable to kill it.
Antimicrobial Activity of Skin Secretion of Rana hosii Frog (Anura: Ranidae) against several pathogenic microbes Feskaharny Alamsjah; Djong Hon Tjong; Zil Fadhillah Rahma
Jurnal Biologi Universitas Andalas Vol 8, No 2 (2020)
Publisher : Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jbioua.8.2.48-53.2020

Abstract

The research on antimicrobial activity of skin secretion derived from Rana hosii (Anura: Ranidae) against several pathogenic microbes had been conducted at Microbiology Laboratory and at Genetics and Biomolecular Laboratory, both in the Biology Department, Universitas Andalas. The research used to survey and experimental methods. The study aimed to determine the effectivity of Rana hosii skin secretion to inhibit microbial activity, hence it tested onto some pathogenic microbes such as Escherichia coli, Staphylococcus aureus and Candida albicans. The results showed that the biggest inhibition zone formed against E. coli was 10.96 + 0.63 mm, on S. aureus was 11.74 + 1.00 mm, while on C. albicans was 7.42 + 0.80 mm. The results indicated that the skin secretions from Rana hosii frog could be a potential broad-spectrum antibacterial, and antifungal activity against C. albicans.
Pengaruh pH dan Suhu terhadap Produksi Antibiotika dari Isolat Bakteri Endofitik pada Tumbuhan Andalas (Morus macroura Miq.) Riski Budi Yani; Anthoni Agustien; Feskaharny Alamsjah
Metamorfosa: Journal of Biological Sciences Vol 6 No 1 (2019)
Publisher : Prodi Magister Ilmu Biologi, Fakultas MIPA, Universitas Udayana

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24843/metamorfosa.2019.v06.i01.p14

Abstract

The study used survey method and the data were analysed descriptively. The selection of the bacteria which produce antibiotic had been with paper disk method and used Escherichia coli and Staphylococcus aureus as the sample bacteria. This result showed pH 7,0 was succesful optimum pH antibiotic produced for Bacillus sp.1 and Bacillus sp.2. and 370C was the optimum temperature to antibiotic produced from Bacillus sp.1 and Bacillus sp.2.
Antibacterial Potential of Actinomycetes Isolates from Imperata (Imperata cylindrica L.) Rhizosfer Against Eschericia coli and Staphylococcus aureus Feskaharny Alamsjah; Apriliana Wahyuni; Kawther Ameen Muhammed Saeed Aledresi
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 22 No. 3 (2021): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (907.281 KB) | DOI: 10.24036/eksakta/vol22-iss3/279

Abstract

Research on the Antibacterial Potential of Actinomycetes Isolates from the Rhizosphere of imperata (Imperata cylindrica L.) Against Eschericia coli and Staphylococcus aureus has been conducted at the Biota Laboratory of Sumatera, Andalas University from July to September 2020. This study aims to obtain actinomycetes isolates from plant rhizosphere reeds (Imperata cylindrica L.) which has the potential as an antibacterial against Escherichia coli and Staphylococcus aureus. This study used a survey method. The results showed that 4 isolated actinomycetes were found, all of which have antibacterial potential, which could inhibit the growth of Escherichia coli and Staphylococcus aureus.
Antibacterial Test of Ziziphus spina-christi (L.) Desf. Leaves Extract Againstgram-Positive Bacteria Feskaharny Alamsjah; Anthoni Agustien; Fadhila Cantika
EKSAKTA: Berkala Ilmiah Bidang MIPA Vol. 24 No. 02 (2023): Eksakta : Berkala Ilmiah Bidang MIPA (E-ISSN : 2549-7464)
Publisher : Faculty of Mathematics and Natural Sciences (FMIPA), Universitas Negeri Padang, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24036/eksakta/vol24-iss02/278

Abstract

Ziziphus spina-christi (L.) Desf. is a member of the Rhamnaceae family, which has efficacy values as herbal plants used in traditional medicines. The spread of infectious diseases in humans has increased in recent years, raising public awareness of the importance of using traditional medicine as the first line of prevention against infectious diseases. This research aimed to determine the antibacterial activity of fresh extract, infusion, and ethanol extract and to determine the greatest inhibition zone of Z. spina-christi leaves against Gram-positive bacteria (B. cereus, B. subtilis, S. aureus, and S. epidermidis). This research used the survey method with a purposive sampling technique. The results showed that fresh extract, infusion, and ethanol extract of the Z. spina-christi leaves had antibacterial activity against Gram-positive bacteria (B. cereus, B. subtilis, S. aureus, and S. epidermidis) and the greatest inhibition zone was obtained from the ethanol extract with a strong inhibition category.
Uji Antibakteri Ekstrak Rimpang Koenih Rimbo (Curcuma sumatrana Miq.) Tumbuhan Endemik Sumatra Barat terhadap Bakteri Gram Positif Feskaharny Alamsjah; Anthoni Agustien; Tri Widia Ningsih Alam
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 1 (2023): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i1.7697

Abstract

Koenih rimbo (Curcuma sumatrana Miq.) is a medicinal plant endemic to Sumatra from the Zingiberaceae family. Research on the antibacterial test of koenih rimbo rhizome extracts against Gram-positive bacteria has been conducted at the Basic Biology Laboratory, UPT. Basic and Central Laboratory, Chemical Laboratory of Natural Materials, UPT. Biological Resources, Andalas University,Padang. This study aims to determine the antibacterial activity of fresh extracts, infusions, and ethanol extracts and determine the largest inhibition zone of koenih rimbo rhizomes against several Gram-positive bacteria. The research used a survey method with a purposive sampling technique. The results showed that the fresh extract, infusa, and ethanol extract had antibacterial activity against Gram-positive bacteria (Staphylococcus aureus, S. epidermidis, Bacillus subtilis, and B. cereus. The most significant inhibition zone was obtained from the extract with ethanol solvent with a strong inhibition category.
Uji Antagonis Bacillus subtilis ATTC 6633 dan Trichoderma harzianum terhadap Pertumbuhan Magnaphorte oryzae pada Benih Padi Anak Daro dengan Variasi Lama Perendaman Feskaharny Alamsjah; Zozy Aneloi Noli; Riesca Salsabilah Rahmayati; Suwirmen Suwirmen; Anthoni Agustien; Kurniadi Ilham
Bioscientist : Jurnal Ilmiah Biologi Vol 11, No 2 (2023): December
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v11i2.9468

Abstract

Magnaphorte oryzae is a pathogenic fungus that causes blast disease in rice plants. This disease can cause a decrease in rice production. Therefore, it is necessary to control the disease, one of which is by administering microbes that are antagonistic to Magnaphorte oryzae. This research aims to determine the percentage of inhibitory power of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the growth of Magnaphorte oryzae with varying soaking times of 24, 48 and 72 hours on the Anak Daro rice variety. This research carried out viability tests for Bacillus subtilis ATTC 6633 and Trichoderma harzianum, as well as antagonist tests using the dual culture method. The results showed that the growth inhibition of Magnaphorte oryzae by Bacillus subtilis ATTC 6633 for a soaking period of 24 hours was 28.48% in the weak category, 48 hours was 32.27% and 72 hours was 31.01% in the medium category. Inhibition of Magnaphorte oryzae by Trichoderma harzianum for a soaking period of 24 hours was 50.63%, 48 hours was 57.59%, and 72 hours was 56.32% in the high category.
Pengaruh Lama Perendaman pada Biopriming Padi Anak Daro Menggunakan Bacillus subtilis dan Trichoderma harzianum Zozy Aneloi Noli; Feskaharny Alamsjah; Riesca Salsabilah Rahmayati
Bioscientist : Jurnal Ilmiah Biologi Vol 12, No 1 (2024): June
Publisher : Department of Biology Education, FSTT, Mandalika University of Education, Indonesia.

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33394/bioscientist.v12i1.10987

Abstract

The majority of Indonesian people consume rice as a staple food. According to data from the Central Statistics Agency (2022), rice production in West Sumatra reached 1,482,996.01 tons in 2019 and continued to decline until 2021, total rice production in West Sumatra was only 1,317,209.38 tons. This is inversely proportional to the population of West Sumatra which increases every year, so efforts need to be made to increase rice production. One of them is the biopriming technique to increase seed vigor. Priming is hydration carried out slowly which aims to ensure that the seed's water potential reaches balance and activates metabolism in the seed before the seed germinates. One of them is biopriming, which is a seed priming technique using biological agents in the form of beneficial microorganisms. Several factors influence the success of the priming technique, one of which is the soaking time. The right soaking time provides a deeper opportunity to absorb water to stimulate the seeds to germinate. This research aims to determine the effect of soaking time on biopriming of the local West Sumatran rice variety "Anak Daro" using Bacillus subtilis and Trichoderma harzianum on the germination of the Anak Daro rice variety. Completely Randomized Design (CRD) factorial pattern with 2 factors with 3 replications. Factor A is a biopriming agent, namely A1 (no microbes), A2 (Bacillus subtilis), and A3 (Trichoderma harzianum). Factor B is the length of soaking, namely B1 (24 hours), B2 (48 hours), and B3 (72 hours). The results showed that Bacillus subtilis and Trichoderma harzianum had a significant effect on maximum growth potential and germination, while soaking time had a significant effect on maximum growth potential and vigor index.
Socialization of BOTAFA: Fauna Hand Puppets as Educational Media for Indonesian Fauna at SDN 22 Kuranji Rafia Rafia; Fajar Irianto; Alivia Zulkarnain; Feskaharny Alamsjah
Warta Pengabdian Andalas Vol 31 No 3 (2024)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat (LPPM) Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25077/jwa.31.3.484-491.2024

Abstract

Human activities pose significant threats to the biodiversity of Indonesia, leading to a decline in the population of endemic animals. Insufficient awareness among the public regarding the importance of conservation exacerbates wildlife trafficking and decreases animal populations. To enhance awareness, early-age education is crucial. Hand puppets, as educational tools, offer an engaging and practical approach to teaching children about Indonesian fauna. The implementation of Fauna Hand Puppetry (FHP) was conducted at SDN 22 Kuranji, and through a series of hand puppets, illustrated storybooks, and QR-coded tags linked to a website, the socialization aimed to capture children's interest and provide easy understanding of the protected fauna. FHP products are designed with child-safe materials and attractively packaged, empowering children to take an active role in conservation. Socialization methods involve PowerPoint (PPT) presentations to explain Indonesian wildlife and interactive Q&A sessions to allow students to inquire and discuss the presented materials. Through a combination of presentations and Q&A sessions, this socialization effort is anticipated to foster a better understanding among students regarding the importance of protecting Indonesian fauna. The quiz and Q&A session results conducted after the socialization showed that students' understanding increased, and they could answer an average of 9 out of 10 questions correctly related to Indonesian fauna.
Antagonist Test of Bacillus subtilis ATTC 6633 and Trichoderma harzianum on the Growth of Magnaphorte oryzae on Several Varieties of Priming Rice Seeds Feskaharny Alamsjah; Zozy Aneloi Noli; Hesti Dwi Marcellinna; Anthoni Agustien; Suwirmen; Kurniadi Ilham
Jurnal Biologi Tropis Vol. 23 No. 1 (2023): Special Issue
Publisher : Biology Education Study Program, Faculty of Teacher Training and Education, University of Mataram, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jbt.v23i1.6112

Abstract

Priming is a seed-soaking technique to increase seed viability and also suppress pathogens' growth. Magnaporthe oryzae is one of the pathogens in rice plants that causes leaf blast disease. The high rice consumption each year in Indonesia is not proportional to the amount of rice plant production, which is affected by the growth of pathogenic fungi. This study aims to determine the viability and percentage inhibition of Bacillus subtilis and Trichoderma harzianum against the growth of the pathogenic fungus Magnaporthe oryzae on local West Sumatra rice varieties Ceredek, Pandan Pulau, and Batang Sungkai. The research was conducted by testing the viability of microbes by counting the number of colonies and testing microbial antagonists with the Dual Culture method on seven days of observation. The results showed that Bacillus subtilis and Trichoderma harzianum could inhibit the growth of the pathogen M. oryzae, which causes leaf blast disease. The viability of Bacillus subtilis after biopriming for 48 hours was most significant on Ceredek variety, which was 15.9x106 cfu/g, and for Trichoderma harzianum on Pandan Pulau variety, which was 0.4x106 cfu/g. The most significant inhibition percentage of Bacillus subtilis and Trichoderma harzianum was obtained in Ceredek rice with a value of 30.13% (medium category) and 63.04% (high category).