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All Journal Fundamental and Applied Research in Medicine and Allied Sciences Indonesia
Husnunnisa
Universitas Muhammadiyah Riau

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Optimization of Dengue-EDIII – Hepatitis B-VLP Fusion Antigen Protein Expression in Mammalian Cell Systems and Preliminary Immunogenicity Testing in Mice Ziza Putri Aisyia Fauzi; Eka Nur Fatmawati; Putri Tri Hartini; Yuneka Saristiana; Husnunnisa
Fundamental and Applied Research in Medicine and Allied Sciences Indonesia Vol. 1 No. 2 (2025): November, 2025
Publisher : CV. Get Press Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.69855/farmasi.v1i2.430

Abstract

The development of a safe and effective dengue vaccine remains challenging due to the risk of antibody-dependent enhancement (ADE) associated with imbalanced immune responses. This study aimed to optimize the expression of a dengue virus envelope domain III (EDIII) fusion antigen displayed on Hepatitis B core antigen virus-like particles (VLPs) and to evaluate its preliminary immunogenicity in a murine model. Optimization of antigen design and expression conditions resulted in a substantial increase in VLP production compared with the non-optimized construct. The purified VLPs showed uniform size, proper particle assembly, and preserved antigenic integrity. Immunization of mice induced high titers of EDIII-specific IgG and strong serotype-specific neutralizing antibodies against dengue virus serotype 2, while no antibody-dependent enhancement was detected in vitro. These findings indicate that the optimized EDIII–HBcAg VLP platform is highly immunogenic and demonstrates a favorable safety profile, supporting its potential as a promising subunit vaccine candidate for dengue virus.
Co-Authors Eka Nur Fatmawati Hartini, Putri Tri Yuneka Saristiana Ziza Putri Aisyia Fauzi