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All Journal Physics and Science Education Journal (PSEJ)
Jeni Khusnelia
Universitas Jambi
Education Physics

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ISOLATION AND CHARACTERIZATION OF LACTIC ACID BACTERIA FROM MANDAI (FERMENTED CEMPEDAK PEEL) IN EAST KALIMANTAN Jeni Khusnelia; Ervan Johan Wicaksana; Nazarudin Nazarudin; Ilham Falani; Afreni Hamidah; Evita Anggereini
Physics and Science Education Journal (PSEJ) Physics and Science Education Journal (PSEJ), Volume 5 Number 2, August 2025
Publisher : Physics Education Departement, Islamic State University Sulthan Thaha Saifuddin Jambi - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30631/6m3ygk79

Abstract

Mandai is a traditional fermented food from East Kalimantan made from the peel of cempedak (Artocarpus champeden). To the best of our knowledge, this study is the first to report the isolation and characterization of lactic acid bacteria (LAB) from mandai. The fermentation process was carried out for 14 days using MRS Agar medium, resulting in 17 bacterial isolates, consisting of nine Gram-negative and eight Gram-positive isolates. Among them, eight Gram-positive isolates were dominant and exhibited typical LAB characteristics, including acid and gas production from glucose, negative catalase activity, non-motility, growth at 48°C but not at 16°C, and the ability to hydrolyze starch, fat, and casein. During fermentation, the pH decreased from 6.8 to 3.8, confirming active lactic acid production. These findings demonstrate that LAB play a crucial role in generating the characteristic sour taste and improving the quality of mandai. Importantly, the identified LAB isolates have potential as starter cultures to enhance the safety, consistency, and nutritional value of this traditional food.
DEVELOPMENT AND EVALUATION OF SCIENTIFIC APPROACH-BASED STUDENT WORKSHEETS FOR TEACHING THE CONCEPT OF FORCE IN FOURTH-GRADE ELEMENTARY SCHOOLS Jeni Khusnelia; Ervan Johan Wicaksana; Nazarudin Nazarudin; Ilham Falani; Afreni Hamidah; Evita Anggereini
Physics and Science Education Journal (PSEJ) Physics and Science Education Journal (PSEJ), Volume 6 Number 2, August 2026
Publisher : Physics Education Departement, Islamic State University Sulthan Thaha Saifuddin Jambi - Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30631/psej.6.2.%f

Abstract

This study aimed to develop and evaluate a scientific approach-based Student Worksheet (SW) for teaching the concept of force to fourth-grade elementary school students. A Research and Development (R&D) design using the ADDIE model was employed, comprising analysis, design, development, implementation, and evaluation stages. The study involved 28 fourth-grade students at SDN 156/IX Muhajirin, Jambi City, Indonesia, during the 2025 academic year. Data were collected through observations, interviews, questionnaires, expert validation, and teacher and student responses. The results showed high validity, with scores of 94.64% from the subject-matter expert and 90% from the media expert. The SW was also highly practical, as indicated by teacher responses (95%), small-group responses (93.75%), and large-group responses (95.63%). Its effectiveness was demonstrated by an increase in the mean score from 59.39 on the pretest to 74.71 on the posttest. A paired-samples t-test indicated a statistically significant improvement in learning outcomes (p < 0.001), while the N-gain score of 0.38 indicated moderate improvement. Overall, the developed SW was valid, highly practical, and effective in supporting elementary science learning on the concept of force.
Co-Authors Afreni Hamidah Ervan Johan Wicaksana Evita Anggereini Ilham Falani