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THE EFFECT OF ECO-ENZYME ON BIOMASS PRODUCTION OF Lemna sp. IN GIANT GOURAMI (Osphronemus gouramy) CULTURE SYSTEMS Naufal Arif Budiman; Saberina Hasibuan; Rodhi Firmansyah
Asian Journal of Aquatic Sciences Vol. 8 No. 3 (2025): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.8.3.427-439

Giant gourami (Osphronemus gouramy) is one of Indonesia's most valuable freshwater fish species; however, its aquaculture development is constrained by slow growth and low survival rates. Improving water quality through the use of aquatic plants such as Lemna sp. and the application of eco-enzymes is essential. The development of Lemna sp. depends on nutrient availability, especially nitrogen (N), phosphate (P), and potassium (K), which can be enhanced by adding an eco-enzyme. This study aimed to evaluate the effect of different eco-enzyme concentrations on Lemna sp. biomass production, specific growth rate, and water quality parameters in giant gourami culture systems. The research was conducted using an experimental method with a Completely Randomized Design (CRD) consisting of five treatments and three replications: P0 (0 ml/L), P1 (1.5 ml/L), P2 (2.5 ml/L), P3 (3.5 ml/L), and P4 (4.5 ml/L) of eco-enzyme concentration. The results showed that eco-enzyme administration significantly influenced Lemna sp. biomass. The best results were observed in treatment P4 (4.5 ml/L), which produced the highest Lemna sp. biomass (132.87 g) and specific growth rate (4.47%). Optimal water quality was recorded in treatment P4, with temperature ranging from 26.1–29.8°C, pH 5.5–8.38, dissolved oxygen 3.10–6.42 mg/L, phosphate 0.3097–0.3872 mg/L, nitrate 0.5517–10.2414 mg/L, and CO₂ 17–40 mg/L. These findings demonstrate that eco-enzyme application at 4.5 mL/L effectively enhances Lemna sp. productivity as a natural biofilter while simultaneously improving giant gourami growth performance and survival

THE EFFECTIVENESS OF Cosmos caudatus IN PREVENTING HYPERGLYCEMIA DUE TO Aeromonas hydrophila INFECTION IN Pangasianodon hypophthalmus Ronal Kurniawan; Windarti Windarti; Irwan Effendi; Asmika Harnalin Simarmata; Efawani Efawani; Rodhi Firmansyah; Nur Ikhlas Syuhada; Okta Rizal Karsih
Asian Journal of Aquatic Sciences Vol. 8 No. 3 (2025): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.8.3.319-326

Cosmos caudatus is a herb that benefits overall health. This study aimed to determine the effect of C. caudatus leaf supplementation in feed on blood glucose levels in catfish (Pangasianodon hypophthalmus) challenged with Aeromonas hydrophila. This study was conducted from March to August 2024 at the Marine Microbiology Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. The method used was an experimental design employing a completely randomized design (CRD) with four treatments and three replications: negative control (NC, no supplementation, no infection), positive control (PC, no supplementation, with A. hydrophila bacterial infection), supplementation with C. caudatus at doses of 10 g/kg (T1), 15 g/kg (T2), and 20 g/kg (T3) of feed, all tested against A. hydrophila. The fish fry used weighed 5.00 ± 1.00 g and were reared for 75 days in 100-L tanks integrated with an aquaponics system. The fish were fed the experimental feed for 60 days before challenge with A. hydrophila bacteria (10⁸ CFU/mL, intramuscular injection) and continued for 14 days after challenge. Blood glucose levels were measured at the beginning (day 1), day 30, pre-challenge (day 60), and post-challenge (day 75). The results showed that adding basil leaves to the feed affected blood glucose (P<0.05). The addition of C. Caudatus leaves maintained glucose homeostasis within the range of 68.33±10.59-79.67±7.77 mg/dL. These findings indicate that C. Caudatus supplementation effectively prevents hyperglycemia caused by A. hydrohila infection in striped catfish and suggest its potential as a natural immunostimulant and metabolic modulator in aquaculture

OPTIMIZATION OF NURSERY TECHNIQUES FOR SILVER POMPANO (Trachinotus blochii) IN BPBL BATAM: ANALYSIS OF GROWTH PERFORMANCE, SURVIVAL RATE, AND WATER QUALITY Imanda Khalidah; Indra Lesmana; Rodhi Firmansyah
Asian Journal of Aquatic Sciences Vol. 8 No. 3 (2025): December
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.8.3.419-426

The production of silver pompano (Trachinotus blochii) in Indonesia has declined sharply, highlighting persistent issues in its nursery stage, particularly the inability to promote rapid growth and high survival rates when water quality fluctuates. This research assessed intensive nursery techniques implemented at Balai Perikanan Budidaya Laut (BPBL) in Batam by systematically testing different stocking densities, feeding schedules, and key water-quality metrics. Over a twenty-one-day culture cycle, observers collected data daily and interpreted it using descriptive statistics. Fish length rose from an initial mean of 2.50 cm to 3.73 cm, and mean weight climbed from 0.50 g to 1.30 g, yielding an average specific growth rate of 2.5% per day, a survival rate of 83.7%, and a feed-conversion ratio of 2.1. During the trial, temperature (27.6 °C), pH (7.81), dissolved oxygen (5.7 mg/L), and salinity (30 ppt) consistently fell within national SNI 7901-2013 standards. Most deaths occurred during grading rather than through cannibalism, indicating that handling stress, rather than predatory behavior, was the primary cause of loss. Collectively, these results establish a reference point for nursery management of T. blochii and furnish Indonesian aquaculture planners with data needed to boost fish-seed output while strengthening coastal community livelihoods

EFFECT OF LIQUID ORGANIC FERTILIZER (LOF) FROM CATFISH SOLID WASTE ON PEAT WATER QUALITY PARAMETERS IN THE CULTIVATION OF CLIMBING PERCH (Anabas testudineus) Rahel Meriani Siringo Ringo; Saberina Hasibuan; Rodhi Firmansyah
Asian Journal of Aquatic Sciences Vol. 9 No. 1 (2026): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.9.1.73-81

Liquid organic fertilizer (LOF) derived from the solid waste of catfish processing is a fermented organic product containing macronutrients such as nitrogen (N), phosphorus (P), and potassium (K), as well as micronutrients, amino acids, and decomposer microorganisms that improve water quality. This study aims to analyze the effects of LOF application on peat water quality and the growth performance of climbing perch (Anabas testudineus), and to determine the optimal dosage for cultivation. The research was conducted from June to July 2025 at the Aquaculture Environmental Quality Laboratory, Faculty of Fisheries and Marine, Universitas Riau, with fish rearing carried out in the hatchery using aquarium. The study employed a Completely Randomized Design (CRD) consisting of five treatments: P0 (0 ml/L), P1 (1.5 ml/L), P2 (2 ml/L), P3 (2.5 ml/L), and P4 (3 ml/L), each with three replications. The results showed that water quality parameters remained within acceptable ranges, with temperature of 28.73–28.9°C, pH of 4.96–5.2, TDS of 108.26–124.6 ppm, transparency of 7.66–9.1 cm, DO of 5.23–5.36 mg/L, alkalinity of 17–28 mg/L, nitrate of 4.0045–8.7292 mg/L, and orthophosphate of 1.5397–3.5454 mg/L. The application of POC increased pH, TDS, nitrate, and orthophosphate concentrations. The best growth performance was observed in treatment P2 (2 ml/L), which produced the highest absolute weight gain (2.15 g) and absolute length growth (2.72 cm), while the survival rate reached 100% across all treatments. Therefore, the P2 dosage is considered the most effective treatment for improving peat water quality and enhancing the growth of climbing perch.

INFLUENCE OF BIOMASS Lemna sp. ON THE PHYSICAL PARAMETERS OF PEAT WATER IN THE REARING MEDIUM OF ASIAN REDTAIL CATFISH (Hemibagrus nemurus) Willy Josevin Manihuruk; Saberina Hasibuan; Rodhi Firmansyah
Asian Journal of Aquatic Sciences Vol. 9 No. 1 (2026): April
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.9.1.53-65

Peatland waters hold great potential for aquaculture development; however, their unfavorable characteristics, such as low pH, brownish water color, and high levels of Total Dissolved Solids (TDS) and Total Suspended Solids (TSS), pose major challenges. One approach to improving peat water quality is the use of aquatic plants as phytoremediation agents, such as Lemna sp. This study aims to determine the effect of Lemna sp biomass on the physical parameters of peat water and to identify the optimal biomass for the rearing of Asian redtail catfish (Hemibagrus nemurus). The study was conducted from March to July 2025 at the Integrated Marine Science Laboratory of the Faculty of Fisheries and Marine Sciences at the Universitas Riau. The research method used was an experiment with a one-factor completely randomized design (CRD), consisting of five Lemna sp biomass treatments (0, 25, 37.5, 50, and 62.5 g/m²) and three replicates. The parameters observed included TDS, TSS, turbidity, temperature, pH, water color, absolute weight gain, and survival rate of Asian redtail catfish. The results showed that the addition of Lemna sp biomass significantly improved (P<0.05) the physical parameters of peat water. Increasing the Lemna sp biomass reduced TDS and TSS values and improved water clarity compared to the control without Lemna sp. These improvements in water quality positively affected the absolute weight gain and survival rate of Asian redtail catfish during the rearing period. A Lemna sp. biomass of 62.5 g/m² yielded the best results, with the most optimal physical quality of peat water and the highest growth and survival rates of Asian redtail catfish.

EFFECTS OF APPLYING LIQUID ORGANIC FERTILIZER FROM NONI FRUIT (Morinda citrifolia L.) ON THE BIOMASS OF Lemna sp. IN THE CULTURE MEDIA FOR NILE TILAPIA (Oreochromis niloticus) Yulisa Rika Sari; Saberina Hasibuan; Rodhi Firmansyah
Asian Journal of Aquatic Sciences Vol. 9 No. 2 (2026): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.9.2.164-174

Lemna sp. is an aquatic plant with high nutritional content, especially protein, and can grow rapidly in favorable environmental conditions. In addition to being a food source, Lemna sp. acts as a phytoremediator by absorbing ammonia, nitrate, and phosphate compounds derived from feed residues and fish metabolism. This study aims to investigate the use of Liquid Organic Fertilizer (LOF) derived from noni fruit to support the growth of Lemna sp. in tilapia fish culture media. The study was conducted from September to October 2025 at the Aquaculture Environmental Quality Laboratory, Faculty of Fisheries and Marine Sciences, Universitas Riau. This study used an experimental method. The experimental design used a Completely Randomized Design (CRD) consisting of 5 treatment levels and 3 replications so that there were a total of 15 treatment units, namely P0: Without giving noni fruit POC, P1: Giving noni fruit LOF 1.75 mL/L, P2: Giving noni fruit LOF 2 ml/L, P3: Giving noni fruit LOF 2.25 ml/L, P4: Giving noni fruit LOF 2.5 ml/L. The results of the study showed that the administration of noni fruit LOF at a dose of 2.5 ml/L was the best treatment for the growth of absolute weight in Lemna sp, with an average absolute weight of 145.66 ± 4.81 g and a specific growth rate of 4.70 ± 0.08% / day. Treatment with this dose was able to support the growth of tilapia for 30 days without commercial feed and only using Lemna sp as a single feed by producing an absolute weight of tilapia of 1.71 ± 0.10 g, an absolute length of 2.8 ± 0.11 cm, a specific growth rate of 3.22 ± 0.07 % / day, and a survival rate of 96%. In addition, the presence of Lemna sp in the maintenance medium acts as a phytoremediation agent, helping maintain stable water quality. Water quality parameters during the study remained within a reasonable range, with temperatures ranging from 26.4–30.1℃, pH 6.0–7.9, DO 4.1–7.2 mg/L, nitrate 0.06–19.05 mg/L, phosphate 0.13–4.16 mg/L, and ammonia 0.01–0.02 mg/L, supporting tilapia survival and growth.

UTILIZATION OF LIQUID ORGANIC FERTILIZER (LOF) FROM GOAT MANURE ON Lemna sp. BIOMASS IN THE MAINTENANCE MEDIA FOR SNAKEHEAD FISH (Channa striata) Ayu Mita Jami Yanti; Saberina Hasibuan; Rodhi Firmansyah
Asian Journal of Aquatic Sciences Vol. 9 No. 2 (2026): August
Publisher : Lembaga Penelitian dan Pengabdian Kepada Masyarakat Universitas Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31258/ajoas.9.2.211-223

Snakehead fish (Channa striata) culture produces waste in the form of leftover feed that can reduce water quality. One approach to improving water quality is the use of the aquatic plant Lemna sp. for phytoremediation. However, Lemna sp. growth requires sufficient nutrients; therefore, liquid organic fertilizer (LOF) derived from goat manure was applied. This study aimed to determine the optimal dose of goat manure LOF for Lemna sp. biomass production in snakehead fish culture water. The study was conducted from September to October 2025 at the Integrated Laboratory of Marine Sciences, Universitas Riau. A completely randomized design with five treatments and three replicates was used. Treatments were P0 (control), P1 (0,5 mL/L), P2 (1,0 mL/L), P3 (1,5 mL/L), and P4 (2,0 mL/L). The results showed that goat manure LOF significantly affected Lemna sp. biomass (P < 0.05). The best treatment was P4, producing the highest biomass (362.58 g/m²) and specific growth rate (6.56%). Application of goat manure LOF also supported snakehead fish growth, resulting in a final mean weight of 3.20 g, mean length of 3.88 cm, daily weight gain of 2.60 g/day, and survival rate of 98%. In total organic matter ranged from 16.43–55.72 mg/L, nitrate 0.1813–10.0229 mg/L, phosphate 0.6991–5.1416 mg/L, CO₂ 5–18 mg/L, DO 4.10–5.21 mg/L, pH 6.2–7.4, and temperature 27.1–28.9°C. These findings indicate that goat manure LOF at 2.0 mL/L effectively increases Lemna sp. biomass, promotes phytoremediation, and improves snakehead fish growth and survival.

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