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All Journal Medical Journal of Indonesia Indonesian Journal of Clinical Pathology and Medical Laboratory (IJCPML) Indonesian Journal of Applied Research (IJAR)
Sukartini, Ninik
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Computer Science & IT Health Professions Medicine & Pharmacology Neuroscience

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EVALUATION OF BLOOD GLUCOSE TESTING USING CONTOUR® PLUS GLUCOMETER Beauty, Venny; Sukartini, Ninik
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 24, No 3 (2018)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v24i3.1340

Abstract

The use of glucometers has been widely recommended to help patients in controlling their blood glucose level. This study compared the blood glucose level measured by Contour® Plus glucometer and Cobas c501 chemistry analyzer, as the reference method. The study design was cross-sectional and conducted in the Dr. Cipto Mangunkusumo Hospital in April 2017. Study materials were 120 capillary blood examined by Contour® Plus glucometer and plasma analyzed by Cobas c501 chemistry analyzer. Precision, correlation, accuracy, and clinical accuracy tests were performed based on ISO 15197:2013, using Parkes error grid analysis. Contour® Plus glucometer yielded a CV of 1.56-2.2%, following the recommendation of the American Diabetes Association, of <5%, there was a strong positive correlation between the glucose level of capillary blood and plasma (r=0.997). Accuracy test based on ISO 15197:2013 showed that 100% of capillary blood glucose deviations were within the ±15 mg/dL range for glucose level <100 mg/dL and ±15% range for glucose level ≥100 mg/dL. Clinical accuracy test with Parkes error grid showed 100% of results were in zone A. Contour® Plus glucometer test results met the ISO 15197:2013 criteria, so the results were proportional to the reference method’s results and clinically acceptable. Contour® Plus glucometer is safe to be used in blood glucose monitoring, as long as careful attention is given to the device specifications.
Proportion of Bacillary and Morphological Indices of Untreated and Treated Suspected Leprosy Slit Skin Smear Indriani Silvia; Parwati, Ida; Andriyoko, Basti; Rachmawati, Banundari; Sukartini, Ninik
Indonesian Journal of Applied Research (IJAR) Vol. 6 No. 1 (2025): Indonesian Journal of Applied Research (IJAR)
Publisher : Universitas Djuanda

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.30997/ijar.v6i1.599

Abstract

Indonesia is the third highest number of leprosy in the world with pockets of high endemicity spread across the archipelago. Leprosy diagnosis in control program include finding of acid fast bacilli (AFB) from slit skin smear (SSS) that assessed for the bacillary index (BI). The solid AFB form assessed for morphological index (MI). This study aimed to analyze the proportion of BI and MI of untreated and treated suspected leprosy SSS. This was a retrospective observational study on BI and MI from 117 suspected leprosy SSS secondary data. Of this, being males was the most prevalent in the age group 19-40 years with 91 untreated (84.6% acid fast bacilli, AFB) and 26 treated (80.8% AFB). Treated 2+ BI still high 23.8% from untreated BI 2+ 23.4%. Untreated MI >50 was found in 25.4% and treated MI >50 is still found in 19.1%. Bacillary index and MI support the diagnosis and detect the transmission of leprosy infection.
Atypical Plasmacyte Morphology in Primary Plasma Cell Leukemia Kosasih, Agus Susanto; Sukartini, Ninik
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol. 32 No. 1 (2025)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v32i1.2378

Abstract

Plasma cell leukemia (PCL) is a scarce hematology malignancy with challenging clinical picture and dismal prognosis. The diagnostic procedure is sometimes complicated and difficult due to its scarcity. Patient was a 54-year-old male who presented with generalized weakness 2 months prior to hospital admission. He had anemia, thrombocytopenia and leukocytosis with 96% blasts. Initial peripheral blood smear showed unspecific cells that turned out to be plasmacytes. Flow cytometry showed positive for CD38, CD138, Kappa, CD43 and CD200, with conclusion of myeloma. Confirmation with serum protein electrophoresis showed gamma migrating paraprotein with 35.5% gamma Ig G, reduced albumin fraction and alpha 1 globulin. There was M-spike on gamma globulin. Serum immunofixation electrophoresis (SIFE) on the next day showed oligoclonal gammopathy (bi-clonal IgG Kappa and monoclonal Kappa light chain). Based on those results, patient was diagnosed with primary plasma cell leukemia. Diagnosis of PCL is often challenging and misleading due to the clinical features resembling multiple myeloma and unspecific morphology of plasma cell. Peripheral plasmacyte >5% with M-spike on gamma globulin in SPE and gammopathy oligoclonal in SIFE (bi-clonal IgG Kappa and monoclonal Kappa light chain) were supported the diagnosis of PCL and confirmed by the positive flow cytometry for CD38, CD138, Kappa, CD43 and CD200. Therefore, utilization of modern diagnostic procedures like flow cytometry is crucial to make the diagnosis of this rare disease
Erythrocyte and iron profiles in soil-transmitted helminth-infected children in a rural area in Banten, Indonesia Sungkar, Saleha; Sukartini, Ninik; Wirastuti, Aulia; Tanjungsari, Dian Wahyu
Medical Journal of Indonesia Vol. 33 No. 1 (2024): March
Publisher : Faculty of Medicine Universitas Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13181/mji.oa.247154

Abstract

BACKGROUND Soil-transmitted helminth (STH) infection, frequently caused by Ascaris lumbricoides, Trichuris trichiura, and hookworms (Ancylostoma duodenale and Necator americanus), often gives rise to malabsorption of nutrients that form hemoglobin (Hb) thereby causing anemia. This study aimed to know the difference in erythrocyte profile in children with and without STH infections. METHODS This cross-sectional study involved 205 children from two Panimbang Jaya elementary schools in Pandeglang, Banten, Indonesia. Blood and stool samples were collected from a previous study conducted from November 2021 to May 2022. Erythrocyte parameters were Hb concentration, erythrocyte count, mean corpuscular volume (MCV), mean corpuscular Hb (MCH), and red cell distribution width (RDW). The iron profile included serum iron, total iron-biding capacity (TIBC), and ferritin. Worm infestation was detected by direct stool microscopical examination. Statistical analysis was performed using SPSS software version 20. RESULTS The prevalence of STH infection in Pandeglang was 44.4%, primarily characterized by mild intensity STH infection (79%). The identified STH species were A. lumbricoides, T. trichiura, and combination of both. The median differences between erythrocyte count, MCV, and MCH, and the mean differences of TIBC and serum iron were not statistically significant (p = 0.388, 0.098, and 0.057, and p = 0.304 and 0.455). However, children with STH infection had lower Hb (12.57 versus 12.95 g/dl) and ferritin (19.60 versus 30.57 µg/dl) levels but higher RDW (13.20 versus 13.10%). CONCLUSIONS A high prevalence of STH infection was identified among schoolchildren, but their erythrocyte profiles were similar regardless of STH infection status.
Co-Authors Agus Susanto Kosasih, Agus Susanto Banundari Rachmawati Basti Andriyoko Beauty, Venny Ida Parwati Indriani Silvia Saleha Sungkar Tanjungsari, Dian Wahyu Wirastuti, Aulia