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All Journal Biodiversitas Journal of Biological Diversity
NURUL KUSUMA DEWI
Department of Biology, Faculty of Teacher Training and Education, Universitas PGRI Madiun. Jl. Setia Budi No. 85, Madiun 63118, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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Short Communication: Bacterial diversity of mangrove ecosystem in Klawalu Sorong, West Papua, Indonesia SUKMAWATI SUKMAWATI; FEBRIANTI ROSALINA; SIPRIYADI SIPRIYADI; NURUL KUSUMA DEWI; MELDA YUNITA; ABDUL RIDHA TAHA SARHAN; YENI RAHAYU; EKO KUSUMAWATI
Biodiversitas Journal of Biological Diversity Vol. 23 No. 3 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230329

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Abstract. Sukmawati S, Rosalina F, Sipriyadi, Dewi NK, Yunita M, Sarhan ART, Rahayu Y, Kusumawati E. 2022. Short Communication: Bacterial diversity of mangrove ecosystem in Klawalu Sorong, West Papua, Indonesia. Biodiversitas 23: 1427-1432. The mangrove ecosystem is a producer of detritus and a source of nutrients and organic matter. Some of the ecological functions of mangrove forests are coastline protector, preventing seawater intrusion, as a habitat for various living creatures, a microclimate regulator, a nursery ground, spawning ground, as well as a feeding ground for various aquatic biota. The mangrove forest ecosystem cannot be separated from the role of microbes in helping the process of soil biochemical cycles. In the biochemical cycle, microbes are able to maintain the availability of macronutrients in the soil. The objective of this study was to identify the diversity of bacteria in the mangrove ecosystem in Klawalu, Sorong City. The research method descriptively described the diversity of bacterial species found in the mangrove ecosystem in Klawalu, Sorong City, West Papua Province. The results indicated that the DNA fragments of the four isolates obtained from this study were around 1300 bp. Meanwhile, the bacterial species obtained were isolated SA3, identified as Bacillus safensis strain C251, isolate SA8 identified as Bacillus amyloliquefaciens strain NO10, isolate SL8 was identified as Clostridium sp. JC336, and isolate SL1 was identified as Bacillus australimaris strain IIHR GAPB01.
Identification of 16S rRNA genes in bacteriocin-producing bacteria SUKMAWATI SUKMAWATI; SIPRIYADI SIPRIYADI; FEBRIANTI ROSALINA; RATNA RATNA; NURUL KUSUMA DEWI; MOHD IHWAN BIN ZAKARIAH
Biodiversitas Journal of Biological Diversity Vol. 25 No. 10 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d251034

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Abstract. Sukmawati S, Sipriyadi, Rosalina F, Ratna, Dewi NK, Zakariah MIB. 2024. Identification of 16S rRNA genes in bacteriocin-producing bacteria. Biodiversitas 25: 3720-3727. The use of safe and effective biopreservatives in the food industry is highly important to extend the product shelf life and maintain the product quality without posing health risks. Bacteriocins, antimicrobial compounds produced by bacteria, offer biopreservatives potential that can inhibit the growth of pathogens and spoilage microorganisms in food products. Bacteriocins as biopreservatives in the food industry due to their antimicrobial properties and potential to enhance food safety and shelf life. Bacteriocins as biopreservatives are stable at high temperatures, which makes them suitable for various food processes. They are also stable across a broad pH range, ensuring their effectiveness in different food environments. This stability under various conditions instills confidence in their effectiveness. This study aimed to identify the 16S rRNA gene of bacteriocin-producing bacteria as an initial step in developing biopreservative candidates. This research method is a descriptive method that describes the types of bacterial species that are capable of producing bacteriocins. Bacteria were isolated from shrimp paste (cincalok), and genetic analysis was performed using PCR and sequencing techniques to identify the 16S rRNA gene. The bacterial samples derived from shrimp paste were identified as Bacillus paramycoides strain MCCC 1A04098 BSH 1, Bacillus albus strain MCCC 1A02146 BSH 2, and Bacillus cereus strain IAM 12605 BSH. All three samples are Gram-positive with bacilli-shaped cells, leading to the conclusion that these bacteria have the potential as biopreservatives.
Co-Authors ABDUL RIDHA TAHA SARHAN EKO KUSUMAWATI FEBRIANTI ROSALINA MELDA YUNITA MOHD IHWAN BIN ZAKARIAH RATNA RATNA SIPRIYADI SIPRIYADI SIPRIYADI SIPRIYADI SUKMAWATI SUKMAWATI SUKMAWATI SUKMAWATI YENI RAHAYU