Article Per Year (5 Year)
p-Index From 2021 - 2026
0.817
P-Index
This Author published in this journals
All Journal Biodiversitas Journal of Biological Diversity Nusantara Bioscience
AHMAD YUNUS
Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret. Jl. Ir. Sutami 36 A, Surakarta 57126, Central Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Other

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Construction of CRISPR/Cas9_gRNA-OsCKX2 module cassette and its introduction into rice cv. Mentik Wangi mediated by Agrobacterium tumefaciens MARINA RUZYATI; ATMITRI SISHARMINI; ANIVERSARI APRIANA; TRI JOKO SANTOSO; EDI PURWANTO; SAMANHUDI SAMANHUDI; AHMAD YUNUS
Biodiversitas Journal of Biological Diversity Vol. 23 No. 5 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230552

Abstract

Abstract. Ruzyati M, Sisharmini A, Apriana A, Santoso TJ, Purwanto E, Samanhudi, Yunus A. 2022. Construction of CRISPR/Cas9_gRNA-OsCKX2 module cassette and its introduction into rice cv. Mentik Wangi mediated by Agrobacterium tumefaciens. Biodiversitas 23: 2679-2689. Mentik Wangi is an aromatic superior rice variety from the tropical japonica group with high posture and low productivity. The tall plant posture causes Mentik Wangi rice to be prone to lodging, resulting in yield loss. Therefore, improvement of plant height and productivity of Mentik Wangi is still required. The sd-1 (OsGA20ox-2) and CKX2 genes are responsible for the semi-dwarf character and high productivity. The study aimed to construct a CRISPR/Cas9 cassette module carrying a gRNA of OsCKX2 gene and introduce this construct to Mentik Wangi rice mediated by an Agrobacterium tumefaciens vector strain LBA4404. The introduction of the CRISPR/Cas9_gRNA-GA20ox-2 cassette plasmid that was constructed in previous research into Mentik Wangi rice was also carried out. The results showed that the CRISPR/Cas9_gRNA-CKX2 cassette module had been successfully constructed using the Golden gate cloning method. The introduction of the CRISPR/Cas9_gRNA-CKX2 and CRISPR/Cas9_gRNA-GA20ox-2 cassette modules into Mentik Wangi rice resulted in 30 putative transformant lines that passed the hygromycin selection. PCR analysis showed that from the 30 transformant lines, 15 lines were positive for the hygromycin resistance gene. Further analysis is necessary to be conducted to identify the occurrence of mutagenesis in the OsCKX2 and GA20ox-2 target genes.
Morphological characters variation of Indonesian accession Echinacea purpurea in response to gamma-ray irradiation Agustina Putri Cahyaningsih; ‪Nita Etikawati‬; Ahmad Yunus
Biodiversitas Journal of Biological Diversity Vol. 23 No. 10 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d231045

Abstract

Abstract. Cahyaningsih AP, Etikawati N, Yunus A. 2022. Morphological characters variation of Indonesian accession Echinacea purpurea in response to gamma-ray irradiation. Biodiversitas 23: 5351-5359. Indonesia was one of the countries that introduced E. purpurea as a medicinal plant. Accessions of E. purpurea that have been successfully cultivated in Indonesia have narrow genetic diversity, lack accession variation, and have almost uniform tillers. This study was conducted to determine the effect of different doses of gamma-ray irradiation on morphological characteristics of E. purpurea accession B2P2TOOT. The experimental design was a Randomized Block Design with three replications, and six doses of gamma-ray irradiation (0, 15 Gy, 20 Gy, 25 Gy, 40 Gy, and 60 Gy) were used. The qualitative morphological data were presented descriptively; quantitative data were analyzed using ANOVA followed by a DMRT test at a 5% level with SPSS 16.0 application. The Dice similarity algorithm analyzed the similarity index, group analysis, and dendrogram construction using the UPGMA method with the NTSYS 2.02 application. Gamma-ray irradiation treatment increased the survival rate of E. purpurea plants grown in tropical lowlands. A dose of 15-60 Gy gamma irradiation did not affect the qualitative morphology of E. purpurea roots, stems, and leaves. Irradiation at doses of 40 Gy and 60 Gy resulted in flowers with more variation in color, overall flower shape, and arrangement of ray floret. Gamma irradiation significantly affected plant height, leaf length, leaf area, flower angle, and the first day of flowering. The 40 and 60 Gy doses resulted in longer leaves with wider leaf surfaces. The dendrogram revealed that E. purpurea irradiation resulted in two main groups, with doses of 40 and 60 Gy forming their groups and increasing morphological variation by 30% compared to controls.
Evaluating the phenotypic responses of CRISPR/Cas9-edited Mentik Wangi rice mutants T1 focusing on GA20ox2 and OsCKX2 gene knockout TILMIIDZAH SALMA FATHIN; ATMITRI SISHARMINI; ANIVERASRI APRIANA; TRI JOKO SANTOSO; AHMAD YUNUS
Biodiversitas Journal of Biological Diversity Vol. 25 No. 8 (2024)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d250852

Abstract

Abstract. Fathin TS, Sisharmini A, Apriana A, Santoso TJ, Yunus A. 2024. Evaluating the phenotypic responses of CRISPR/Cas9-edited Mentik Wangi rice mutants T1 focusing on GA20ox2 and OsCKX2 gene knockout. Biodiversitas 25: 2783-2790. Mentik Wangi is a local Indonesian rice variety with tall plant stature, making it susceptible to lodging and yield loss, necessitating trait improvements to overcome these weaknesses. Gene editing of GA20ox2 and OsCKX2 using CRISPR/Cas9 was conducted to improve the characteristics of Mentik Wangi. This study aimed to evaluate the phenotypic responses of T1 Mentik Wangi mutant lines generated through gene editing using the CRISPR/Cas9 system. Eighty-five mutant lines and wild-type plants were grown and observed in a greenhouse. Phenotypic traits were observed as quantitative traits, such as plant height, panicle length, total number of tillers, number of productive tillers, total number of grains, 1000-grain weight, heading date, and harvesting age; and qualitative traits, such as panicle type and panicle exsertion type. The results showed a decrease in plant height and panicle length in Mentik Wangi rice mutant lines. Several lines exhibited improvements in total tiller number, productive tiller number, total grain number, and 1000-grain weight. Mutant lines required longer to reach the generative phase and displayed differences in panicle type and panicle exsertion type. This study reported that the T1 mutant lines demonstrated distinct phenotypic responses indicating improvements in the desired traits.
Gamma-ray irradiation induced polymorphism in Echinacea purpurea revealed by RAPD markers AGUSTINA PUTRI CAHYANINGSIH; NITA ETIKAWATI; AHMAD YUNUS
Nusantara Bioscience Vol. 17 No. 2 (2025)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n170211

Abstract

Abstract. Cahyaningsih AP, Etikawati N, Yunus A. 2025. Gamma-ray irradiation induced polymorphism in Echinacea purpurea revealed by RAPD markers. Nusantara Bioscience 17: 289-297. One of the major challenges in the development and cultivation of Echinacea purpurea in Indonesia is the narrow genetic diversity and the lack variation of local accessions, which restricts breeding potential to produce superior varieties with improved traits in terms of morphological characteristics and phytochemical content as medicinal ingredients. To overcome this challenge, gamma-ray irradiation methods can be used as an effective tool to induce mutations and increase genetic variability. This study investigated the impact of gamma irradiation on the genetic diversity of E. purpurea using Random Amplified Polymorphic DNA (RAPD) markers. The seeds of E. purpurea were irradiated using gamma-ray with irradiation doses of 0 Gy (control), 20 Gy, 40 Gy, and 60 Gy. Nine RAPD primers, including OPA-10, OPA-16, OPA-18, and OPA-19, were used to amplify DNA segments. The binary data obtained from the electrophoresis visualization for each treatment were analyzed using the Dice similarity index to calculate the similarity index. Dendrogram construction were performed using the NTSYS. The analysis revealed a significant increase in genetic diversity at doses of 40 Gy and 60 Gy, with 58.04% of the total bands showing polymorphism across all treatments. The 60 Gy treatment, in particular, resulted in the highest genetic dissimilarity compared to the control, indicating a dose-dependent response. The similarity coefficients between control and irradiated plants ranged from 63.7% (20 Gy) to 84.4% (0 Gy), with a noticeable trend toward greater genetic differentiation as the irradiation dose increased. These findings suggest that gamma irradiation effectively induces genetic variation in E. purpurea, which could be harnessed for mutation breeding programs aimed at improving desirable traits such as phytochemical production. However, further studies with larger sample sizes and long-term evaluation are needed to assess the stability of these mutations and their potential for incorporation into breeding programs. This study provides preliminary evidence supporting the use of gamma irradiation in enhancing genetic diversity and its potential application in breeding superior E. purpurea varieties with improved agronomic or medicinal traits.
Co-Authors Agustina Putri Cahyaningsih AGUSTINA PUTRI CAHYANINGSIH ANIVERASRI APRIANA ANIVERSARI APRIANA ATMITRI SISHARMINI ATMITRI SISHARMINI EDI PURWANTO MARINA RUZYATI NITA ETIKAWATI SAMANHUDI SAMANHUDI TILMIIDZAH SALMA FATHIN TRI JOKO SANTOSO TRI JOKO SANTOSO ‪Nita Etikawati‬