Article Per Year (5 Year)
p-Index From 2021 - 2026
0.61
P-Index
This Author published in this journals
All Journal Biodiversitas Journal of Biological Diversity
ADIANA MUTAMSARI WITANINGRUM
Department of Veterinary Public Health, Faculty of Veterinary Medicine, Universitas Airlangga. Jl. Mulyorejo, Kampus C FKH-UNAIR, Surabaya 60115, East Java, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia SHEILA MARTY YANESTRIA; FIDI NUR AINI EKA PUJI DAMEANTI; BETI GISTAWATI MUSAYANNAH; JUNIANTO WIKA ADI PRATAMA; ADIANA MUTAMSARI WITANINGRUM; MUSTOFA HELMI EFFENDI; EMMANUEL NNABUIKE UGBO
Biodiversitas Journal of Biological Diversity Vol. 23 No. 9 (2022)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d230911

Abstract

Abstract. Yanestria SM, Dameanti FNAEP, Musayannah BG, Pratama JWA, Witaningrum AM, Effendi MH, Ugbo EN. 2022. Antibiotic resistance pattern of Extended-Spectrum ?-Lactamase (ESBL) producing Escherichia coli isolated from broiler farm environment in Pasuruan district, Indonesia. Biodiversitas 23: 4460-4465. Escherichia coli is one of the bacteria that can be used as an indicator of environmental pollution. This bacterium has the ability to become an antimicrobial-resistant bacterium that impacts public health. The antimicrobial ability can develop into a bacterium that produces extended-spectrum ?-lactamase (ESBL). The purpose of this study was to reveal the occurrence of extended-spectrum ?-lactamase (ESBL) producing E. coli from a broiler farm environment in Pasuruan district and to describe the phenotypic pattern of E. coli producing ESBL that has been detected. A total of 175 samples were used in this study consisting of 115 samples of coop swabs and 65 samples of wastewater around the farm. The samples were isolated and identified to find E. coli by using different culture media viz. McConkey agar (MCA), eosin methylene blue agar (EMBA), Gram staining, indole test, methyl red Voges Proskauer (MR-VP), citrate, and triple sugar iron agar (TSIA). Detection of ESBL using the double disc synergy test (DDST) according to standard Clinical and Laboratory Standards Institute procedures and the VITEK®2 compact apparatus. The results of ESBL confirmation with DDST and VITEK®2 showed that 16 (9.14%) of the 175 environmental samples confirmed E. coli produced ESBL. The results of the VITEK®2 test also produced a phenotypic pattern of resistance properties of ESBL-producing E. coli and found 12 types of resistance patterns. The combination of “AM AMP ATM KZ CTX CRO CIP” and “AM AMP ATM KZ CTX CRO GM CIP SXT” are the 2 most common resistance patterns (18.75%), while the other 10 resistance patterns occur at the same level (6.25%). The data presented here confirmed the presence of ESBL-producing E. coli in the farm environment, which can contribute to the dissemination of MDR bacteria in the environment if not monitored. Therefore, the presence of ESBL-producing E. coli in Pasuruan is worrisome since it can lead to an impact on human health.
Poultry slaughterhouse wastewater as reservoirs for spreading extended-spectrum beta-lactamase-producing Escherichia coli in Abakaliki, Nigeria EMMANUEL NNABUIKE UGBO; JENNIFER INIOBONG JACOB; MUSTOFA HELMI EFFENDI; ADIANA MUTAMSARI WITANINGRUM; BERNARD NNABUIFE AGUMAH; AGATHA IFUNANYA UGBO; BENJAMIN IKECHUKWU MOSES
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240939

Abstract

Abstract. Ugbo EN, Jacob JI, Effendi MH, Witaningrum AM, Agumah BN, Ugbo AI, Moses BI. 2023. Poultry slaughterhouse wastewater as reservoirs for spreading extended-spectrum beta-lactamase-producing Escherichia coli in Abakaliki, Nigeria. Biodiversitas 24: 4960-4966. The emergence of antimicrobial and multi-resistance extended-spectrum beta-lactamase-producing organisms (ESBL) from the environment and hospitals is a serious global health concern. This research focused on the role of poultry slaughterhouse wastewater as a reservoir for spreading extended-spectrum beta-lactamase-producing Escherichia coli. Exactly 110 poultry slaughterhouses wastewater was collected using sterile universal containers from abattoirs in Abakaliki. The samples were analyzed microbiological for the presence of E. coli using standard techniques. The E. coli isolates found were subjected to a sensitivity test to antibiotics using the Kirby-Bauer disc diffusion method and further tested for ESBL-producing E. coli using a double disc synergy test and chromogenic media Brilliance ESBL agar. Of 110 wastewater samples analyzed, 55 (50.0%) were positive for E. coli contamination. The E. coli isolates showed antibiotic resistance ranging between 21.8% (amoxicillin clavulanic acid) to 69.1% (tetracycline), and good susceptibility was observed on cefepime (72.7%). Thus, among 55 E. coli isolates, 11(20.0%) were ESBL-producing E. coli with resistance patterns as follows: TET-STX-CAZ-CTX-FEP (four isolates); TET-STX-CAZ-CTX (four isolates); TET-STX-CAZ-CTX-FEP-CRO (three isolates), and average multidrug resistance index of 0.7. This study revealed that poultry slaughterhouse wastewater was a reservoir for multidrug resistance ESBL-producing E. coli. Therefore, properly treating wastewater from poultry production or farms before disposing into the drainage channel or water bodies is highly recommended to avoid spreading ESBL-producing organisms from animal waste products to humans and their environment.
Antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria EMMANUEL NNABUIKE UGBO; MUSTOFA HELMI EFFENDI; ADIANA MUTAMSARI WITANINGRUM; WIWIEK TYASNINGSIH; BERNARD NNABUIFE AGUMAH; AGATHA IFUNANYA UGBO; CHARITY CHINYERE NNABUGWU; DIVINEGIFT OLUCHI OKATA-NWALI
Biodiversitas Journal of Biological Diversity Vol. 24 No. 9 (2023)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d240965

Abstract

Abstract. Ugbo EN, Effendi MH, Witaningrum AM, Tyasningsih W, Agumah BN, Ugbo AI, Nnabugwu CC, Okata-Nwali DO. 2023. Antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria. Biodiversitas 24: 5207-5214. Salmonella is a zoonotic foodborne pathogen that can cause serious illness in humans and animals worldwide. Antimicrobial resistance caused by microorganisms has greatly challenged veterinary and human medicine regarding disease treatments. The objective of this study was to evaluate the antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria. One hundred eighty samples (90 broiler feces; 90 broiler cloacal swabs) were collected from three (3) poultry farms in the Abakaliki metropolis. Samples were analyzed and identified for the presence of Salmonella isolates using standard microbiological and biochemical analysis. The antimicrobial sensitivity test of Salmonella isolates against the selected antibiotics was done using the Kirby-Bauer disk diffusion method on Mueller Hinton agar. The results showed 61 samples (33.9 %) were positive for Salmonella. Feces and cloacal swab samples positive for Salmonella were 46.7% and 21.1%, respectively. Multidrug resistance Salmonella (MDR) strains had an overall prevalence of samples (16.2%), i.e., 7 samples of broiler feces had 7 (16.7%) and 3 samples of cloacal swab (15.8%). Thus, all the MDR Salmonella isolates were 100% resistant to tetracycline and ampicillin. The multiple antibiotic-resistant indexes of the MDR were 0.51. This study indicated that broilers chicken and their products were potential sources of human salmonellosis and sources for transmitting MDR Salmonella in Abakaliki, Nigeria. Poultry farmers should use antibiotics in appropriate dosages in their poultry farms.
Co-Authors AGATHA IFUNANYA UGBO AGATHA IFUNANYA UGBO BENJAMIN IKECHUKWU MOSES BERNARD NNABUIFE AGUMAH BERNARD NNABUIFE AGUMAH BETI GISTAWATI MUSAYANNAH CHARITY CHINYERE NNABUGWU DIVINEGIFT OLUCHI OKATA-NWALI EMMANUEL NNABUIKE UGBO EMMANUEL NNABUIKE UGBO EMMANUEL NNABUIKE UGBO FIDI NUR AINI EKA PUJI DAMEANTI JENNIFER INIOBONG JACOB JUNIANTO WIKA ADI PRATAMA MUSTOFA HELMI EFFENDI SHEILA MARTY YANESTRIA WIWIEK TYASNINGSIH